Objective To develop a colorimetric analysis method based on nanozymes, rapidly evaluate the total antioxidant capacity of wild edible mushrooms (Auricularia cornea, Flammulina filiformis, and Pleurotus ostreatus) in Yangzhou. Methods Nano silver modified manganous oxide (Mn/Ag NPs) nanozyme was prepared by the hydrothermal method, and its microstructure was confirmed by various characterization methods. The total antioxidant capacity of 3 kinds of wild edible mushrooms were extracted using different solvents, and their total antioxidant capacity were evaluated by the colorimetric analysis method based on Mn/Ag NPs nanozyme. In addition, the evaluation results were verified using the commercial 2,2’-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) kit. Results The colorimetric analysis method based on Mn/Ag NPs nanozyme achieved the rapid evaluation of the total antioxidant capacity of wild edible mushrooms in Yangzhou. Total antioxidant activity was Pleurotus ostreatus>Flammulina filiformis>Auricularia cornea. Extraction efficiency of solvent was ultrapure water>mixed solvent>ethanol>ethyl acetate. Conclusion The colorimetric analysis method based on Mn/Ag NPs nanozyme can rapidly evaluate the total antioxidant capacity of wild edible mushrooms in Yangzhou.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |