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Rapid detection of acetamiprid in vegetables using colloidal gold immunochromatography assay
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Dong-Sheng FENG1, Yan XU2, Rou-Han CHEN1, Ying-Qing MA1, Meng-Feng GAO1, Xiao-Jun YANG1, Wei WU3, Bo DENG1, *, Na LIU2, *
Journal of Food Safety & Quality | 2025, 16(5) : 179 - 186
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Journal of Food Safety & Quality | 2025, 16(5): 179-186
• Special Topic: Research and Detection of Pesticide and Veterinary Drug Residue •
Rapid detection of acetamiprid in vegetables using colloidal gold immunochromatography assay
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Dong-Sheng FENG1, Yan XU2, Rou-Han CHEN1, Ying-Qing MA1, Meng-Feng GAO1, Xiao-Jun YANG1, Wei WU3, Bo DENG1, *, Na LIU2, *
Affiliations
  • 1. Shanghai Agricultural Products Quality and Safety Center, Shanghai 201708, China
  • 2. Shanghai Institute of Nutrition and Health, University of Chinese Academy of Science, Shanghai 200031, China
  • 3. Shanghai Youlong Biotechnology Co., Ltd., Shanghai 201114, China
Published: 2025-03-15 doi: 10.19812/j.cnki.jfsq11-5956/ts.20241115003
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Objective To prepare colloidal gold immunochromatographic test strips for the rapid detection of acetamiprid residues in vegetables. Methods The immunogen was obtained through hapten synthesis, and a highly sensitive and specific monoclonal antibody against acetamiprid was developed using animal immunization and hybridoma technology. Based on this antibody, parameters such as membrane-coating conditions were optimized to prepare immunocolloidal gold test strips. These test strips, combined with colorimetric analysis, were applied for the quantitative detection of acetamiprid residues in various vegetables. Results Under optimal working conditions, the established method achieved a limit of detection of 0.23 μg/kg for acetamiprid, with a linear range of 0.42-18.38 μg/kg. The recovery rates for actual sample detection ranged from 70.0% to 88.3%, and the coefficients of variation (CV) for intra-batch and inter-batch experiments were below 12.00% and 11.03%, respectively. Conclusion The rapid test strips can be directly applied for high-throughput on-site screening of acetamiprid residues in vegetables. Additionally, with the assistance of a colorimetric analyzer, quantitative acetamiprid detection can also be achieved.

acetamiprid  /  monoclonal antibody  /  gold immunochromatography assay  /  colorimetric analyzer  /  quantitative determination
Dong-Sheng FENG, Yan XU, Rou-Han CHEN, Ying-Qing MA, Meng-Feng GAO, Xiao-Jun YANG, Wei WU, Bo DENG, Na LIU. Rapid detection of acetamiprid in vegetables using colloidal gold immunochromatography assay[J]. Journal of Food Safety & Quality, 2025 , 16 (5) : 179 -186 . DOI: 10.19812/j.cnki.jfsq11-5956/ts.20241115003
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Year 2025 volume 16 Issue 5
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doi: 10.19812/j.cnki.jfsq11-5956/ts.20241115003
  • Receive Date：2024-11-15
  • Online Date：2025-07-19
  • Published：2025-03-15
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  • Received：2024-11-15
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Affiliations
    1. Shanghai Agricultural Products Quality and Safety Center, Shanghai 201708, China
    2. Shanghai Institute of Nutrition and Health, University of Chinese Academy of Science, Shanghai 200031, China
    3. Shanghai Youlong Biotechnology Co., Ltd., Shanghai 201114, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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