Full
Outline
To establish a method for the determination of vitamin B1 in fresh fruit matrix by ultra performance liquid chromatography and optimize the derivatization and extraction conditions.
Vitamin B1 in fruit samples was derivatized with alkaline potassium ferricyanide and then extracted using n-butanol. Chromatographic separation was performed on an ACQUITY UPLC BEH C18 column (2.1 mm×100 mm, 1.7 µm). The mobile phase consisted of 50 mmol/L sodium acetate solution and acetonitrile with gradient elution. The flow rate was set at 0.25 mL/min, and the column temperature was maintained at 40 ℃. The excitation and emission wavelengths were 375 nm and 435 nm, respectively, and the injection volume was 1 μL. Qualitative analysis was conducted based on chromatographic retention time, and quantitative detection was carried out via external standard method with standard curve.
Under the best conditions, vitamin B1 showed a good linear relationship with peak area within the mass concentration range of 0.05–1.00 μg/mL, the correlation coefficient was greater than 0.9998, and the limit of quantitation and limit of detection were 0.80 mg/kg and 0.30 mg/kg, respectively. The accuracy and precision of the method were investigated by using different kinds of fruits as the substrate with low, medium and high concentration levels. The average recovery of vitamin B1 was 79.6%–112.0%, and the relative standard deviation was less than 4.7%.
The established method is applied to the determination of vitamin B1 in various fruit samples. This method features simple and rapid sample pretreatment, high accuracy and favorable precision, and is suitable for the rapid determination of vitamin B1 in large batches of fruit samples.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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