Home Journals Articles Updates About
CN
image
收藏切换
Optimization of the culture medium and screening of freeze-drying protectants for Pediococcus acidilactici TC-4
收藏切换
Zi-Yao ZHANG1, 2, Yu HAO1, 2, Ling CHEN2, 3, Ying LI2, 3, Xin-Qiang XIE2, Ting-Ting LIANG2, Chao-Xin MAN1, *, Qing-Ping WU1, 2, *
Journal of Food Safety & Quality | 2026, 17(10) : 231 - 243
Less
收藏切换
Journal of Food Safety & Quality | 2026, 17(10): 231-243
• Food Analysis and Detection •
Optimization of the culture medium and screening of freeze-drying protectants for Pediococcus acidilactici TC-4
Full
Zi-Yao ZHANG1, 2, Yu HAO1, 2, Ling CHEN2, 3, Ying LI2, 3, Xin-Qiang XIE2, Ting-Ting LIANG2, Chao-Xin MAN1, *, Qing-Ping WU1, 2, *
Affiliations
  • 1.College of Food Science, Northeast Agricultural University, Harbin 150030, China
  • 2.State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Safety and Health, National Health Commission Science and Technology Innovation Platform for Nutrition and Safety of Microbial Food, Key Laboratory of Big Data Technologies for Food Microbiological Safety, State Administration for Market Regulation, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China
  • 3.Guangdong Microbial Analysis and Detection Center,Guangzhou 510070, China
doi: 10.19812/j.cnki.jfsq11-5956/ts.20260319004
Outline
收藏切换
Objective

To optimize the composition of the culture medium for Pediococcus acidilactici TC-4 and the formula of freeze-drying protectants.

Methods

The composition and mass concentration of the culture medium for Pediococcus acidilactici TC-4 and the formula of the freeze-drying protectant were screened by combining single-factor experiments, Plackett-Burman experiments and response surface methodology.

Results

The optimal composition of the culture medium was: Glucose 26 g/L, yeast extract 30 g/L, sodium acetate 8 g/L, diammonium hydrogen citrate 2 g/L, dipotassium phosphate 2 g/L, magnesium sulfate 0.4 g/L, manganese sulfate 0.075 g/L and Tween 80 1 g/L. The viable count increased from 3.50×109 CFU/mL to 1.09×1010 CFU/mL, which was 3.11 times that before optimization. The optimal formula of the freeze-drying protectant was: Sodium L-glutamate 8%, maltitol 14%,skim milk powder 18%, and the survival rate reached 90.77%.

Conclusion

The optimization of the culture medium for Pediococcus acidilactici TC-4 is completed, and a high-viable probiotic preparation is successfully prepared, lays a solid theoretical foundation for the industrialization of this strain.

Pediococcus acidilactici  /  hyperuricemia  /  culture medium  /  freeze-drying protectant
张仔瑶, 郝宇, 陈玲, 李滢, 谢新强, 梁婷婷, 满朝新, 吴清平. 乳酸片球菌TC-4的培养基优化及冻干保护剂筛选. 食品安全质量检测学报, 2026 , 17 (10) : 231 -243 . DOI: 10.19812/j.cnki.jfsq11-5956/ts.20260319004
Zi-Yao ZHANG, Yu HAO, Ling CHEN, Ying LI, Xin-Qiang XIE, Ting-Ting LIANG, Chao-Xin MAN, Qing-Ping WU. Optimization of the culture medium and screening of freeze-drying protectants for Pediococcus acidilactici TC-4[J]. Journal of Food Safety & Quality, 2026 , 17 (10) : 231 -243 . DOI: 10.19812/j.cnki.jfsq11-5956/ts.20260319004
Appendix
Less
Year 2026 volume 17 Issue 10
11
2
Cite this Article
BibTeX
Article Info
doi: 10.19812/j.cnki.jfsq11-5956/ts.20260319004
  • Receive Date：2026-03-19
  • Online Date：2026-11-05
Article Data
Affiliations
History
  • Received：2026-03-19
Funding
Affiliations
    1.College of Food Science, Northeast Agricultural University, Harbin 150030, China
    2.State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Safety and Health, National Health Commission Science and Technology Innovation Platform for Nutrition and Safety of Microbial Food, Key Laboratory of Big Data Technologies for Food Microbiological Safety, State Administration for Market Regulation, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China
    3.Guangdong Microbial Analysis and Detection Center,Guangzhou 510070, China
References
Share
https://castjournals.cast.org.cn/joweb/spaq/EN/10.19812/j.cnki.jfsq11-5956/ts.20260319004
Share to
QR

Scan QR to access full text

Cite this article
BibTeX
Citations
表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
  • BibTeX
  • EndNote
  • RefWorks
  • TxT
Links
Articles
Latest Articles
Most Read
Collections
Updates
Events
News
Multimedia
About
About Us
Contact
No. 86 Xueyuan South Road, Haidian District, Beijing
100081
010-62662699(Operation and Technical Support)
qkjq@cast.org.cn

Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House