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Establishment of detection methods for 12 kinds of animal-derived ingredients and development of reference materials
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Qing-Zi DUAN1, 2, 3, Ke SHANG1, 2, 3, Yuan REN1, 2, 3, Wei WANG1, 2, 3, Hong ZHU1, 2, 3, Lei-Lei CHEN4, Min ZHANG1, 2, 3, Dai-Wei CHEN1, 2, 3, Yi WANG1, 2, 3, Hong-Yang ZHAO1, 2, 3, *
Journal of Food Safety & Quality | 2026, 17(14) : 51 - 59
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Journal of Food Safety & Quality | 2026, 17(14): 51-59
• Special Topic: Processing and Quality Safety of Animal Food •
Establishment of detection methods for 12 kinds of animal-derived ingredients and development of reference materials
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Qing-Zi DUAN1, 2, 3, Ke SHANG1, 2, 3, Yuan REN1, 2, 3, Wei WANG1, 2, 3, Hong ZHU1, 2, 3, Lei-Lei CHEN4, Min ZHANG1, 2, 3, Dai-Wei CHEN1, 2, 3, Yi WANG1, 2, 3, Hong-Yang ZHAO1, 2, 3, *
Affiliations
  • 1.Chengdu Institute of Food Inspection, Chengdu 611130, China
  • 2.Key Laboratory of Monitoring and Assessment on Novel Food Raw Materials, State Administration for Market Regulation, Chengdu 611130, China
  • 3.Key Laboratory of Chemical Metrology and Applications on Nutrition and Health, State Administration for Market Regulation, Beijing 100029, China
  • 4.Institute of Food & Nutrition Science and Technology, Shandong Academy of Agricultural Science, Ji’nan 250100, China
doi: 10.19812/j.cnki.jfsq11-5956/ts.20260311001
Outline
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Objective

To establish a method for the simultaneous detection of 12 kinds of animal-derived ingredients in food, including Sus scrofa, Bos taurus, Bubalus bubalis, Bos grunniens, Capra hircus, Ovis aries, Gallus gallus, Anas platyrhynchos, Anser cygnoides, Equus asinus, Equus caballus, Oryctolagus cuniculus by high-throughput quantitative real-time polymerase chain reaction (qPCR), and develop corresponding reference materials.

Methods

Primers, probes and reaction system were designed based on specific target genes of each animal-derived ingredient. The sensitivity and specificity of the method were evaluated. Concurrently, corresponding reference materials were developed.

Results

A qPCR system for the simultaneous detection of 12 kinds of animal-derived ingredients was successfully established. The sensitivity for each ingredient was 0.01 ng/μL, and no amplification was observed in non-target species,demonstrating good specificity. The limit of detection of this method for different animal-derived components in fresh meat was 0.1%. The developed reference materials exhibited good homogeneity and stability, with the assigned value being positive for the corresponding animal-derived ingredients. The shelf life under frozen conditions at –18 °C is up to 36 months.

Conclusion

The established method exhibits good specificity and high sensitivity, effectively improving detection efficiency and reducing costs. The prepared reference materials meet the requirements for reference materials and can be used for intended purposes such as quality assurance throughout the detection process.

meat and meat products  /  animal-derived ingredients  /  quantitative real-time polymerase chain reaction  /  reference material
段庆梓, 尚柯, 任缘, 王巍, 朱宏, 陈蕾蕾, 张敏, 陈代伟, 王义, 赵红阳. 12种动物源性成分检测方法的建立及标准样品研制. 食品安全质量检测学报, 2026 , 17 (14) : 51 -59 . DOI: 10.19812/j.cnki.jfsq11-5956/ts.20260311001
Qing-Zi DUAN, Ke SHANG, Yuan REN, Wei WANG, Hong ZHU, Lei-Lei CHEN, Min ZHANG, Dai-Wei CHEN, Yi WANG, Hong-Yang ZHAO. Establishment of detection methods for 12 kinds of animal-derived ingredients and development of reference materials[J]. Journal of Food Safety & Quality, 2026 , 17 (14) : 51 -59 . DOI: 10.19812/j.cnki.jfsq11-5956/ts.20260311001
Appendix
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Year 2026 volume 17 Issue 14
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Article Info
doi: 10.19812/j.cnki.jfsq11-5956/ts.20260311001
  • Receive Date：2026-03-11
  • Online Date：2026-11-05
Article Data
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  • Received：2026-03-11
Funding
Affiliations
    1.Chengdu Institute of Food Inspection, Chengdu 611130, China
    2.Key Laboratory of Monitoring and Assessment on Novel Food Raw Materials, State Administration for Market Regulation, Chengdu 611130, China
    3.Key Laboratory of Chemical Metrology and Applications on Nutrition and Health, State Administration for Market Regulation, Beijing 100029, China
    4.Institute of Food & Nutrition Science and Technology, Shandong Academy of Agricultural Science, Ji’nan 250100, China
References
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https://castjournals.cast.org.cn/joweb/spaq/EN/10.19812/j.cnki.jfsq11-5956/ts.20260311001
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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