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Rapid detection of nicarbazine in chicken by colloidal gold immunochromatography
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Yun-Ting YI1, Tao HOU1, *, Qiu-Xiong YE1, Jun-Fa LIANG1, Jia-Jian LIN1, Zi-Hong HUANG1, Liang-Yu ZHU1, Liang-Liang CHEN1, Ding-Feng LUO1, Ya-Qing LIU2, Ke LIANG3
Journal of Food Safety & Quality | 2026, 17(7) : 251 - 256
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Journal of Food Safety & Quality | 2026, 17(7): 251-256
• Special Topic: Rapid Detection Technology for Food Safety •
Rapid detection of nicarbazine in chicken by colloidal gold immunochromatography
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Yun-Ting YI1, Tao HOU1, *, Qiu-Xiong YE1, Jun-Fa LIANG1, Jia-Jian LIN1, Zi-Hong HUANG1, Liang-Yu ZHU1, Liang-Liang CHEN1, Ding-Feng LUO1, Ya-Qing LIU2, Ke LIANG3
Affiliations
  • 1.Guangzhou Institute of Food Inspection, Guangzhou 511405, China
  • 2.School of Life and Health Technology,Dongguan University of Technology, Dongguan 523808, China
  • 3.Guangdong Dayuan Oasis Food Safety Technology Co., Ltd., Guangzhou 510700, China
doi: 10.19812/j.cnki.jfsq11-5956/ts.20251009003
Outline
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Objective

To establish a method for the rapid detection of the nicarbazine residue marker 4, 4’-dinitrogendibenzuron in chicken by colloidal gold immunochromatography.

Methods

Key parameters including extraction solvent, volume and incubation time were systematically optimized to determine the optimal detection conditions: 10.0 mL of methanol as the extraction solvent, an extraction time of 2 min and an incubation time of 3 min.

Results

Experimental validation demonstrated that the method exhibited cross-reactivity rates of less than 0.1% with 15 kinds of common anticoccidial drugs (e.g., diclazuril, salinomycin) and 5 kinds of structural analogs, indicating excellent specificity. When applied to real sample analysis, the results showed good agreement with those obtained by the reference method, with a false negative rate, false positive rate and relative accuracy of 0%, 0% and 100%, respectively.

Conclusion

The developed method is simple, rapid, sensitive and specific, making it suitable for on-site rapid screening of nicarbazin residues in chicken.

nicarbazine  /  4,4’-dinitrocarbanilide  /  colloidal gold immunochromatographic  /  rapid detection
易云婷, 侯韬, 叶秋雄, 梁俊发, 林嘉健, 黄子泓, 朱亮宇, 陈亮亮, 罗鼎峰, 刘亚青, 梁科. 胶体金免疫层析法快速检测鸡肉中尼卡巴嗪. 食品安全质量检测学报, 2026 , 17 (7) : 251 -256 . DOI: 10.19812/j.cnki.jfsq11-5956/ts.20251009003
Yun-Ting YI, Tao HOU, Qiu-Xiong YE, Jun-Fa LIANG, Jia-Jian LIN, Zi-Hong HUANG, Liang-Yu ZHU, Liang-Liang CHEN, Ding-Feng LUO, Ya-Qing LIU, Ke LIANG. Rapid detection of nicarbazine in chicken by colloidal gold immunochromatography[J]. Journal of Food Safety & Quality, 2026 , 17 (7) : 251 -256 . DOI: 10.19812/j.cnki.jfsq11-5956/ts.20251009003
Appendix
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Year 2026 volume 17 Issue 7
13
3
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Article Info
doi: 10.19812/j.cnki.jfsq11-5956/ts.20251009003
  • Receive Date：2025-10-09
  • Online Date：2026-11-05
Article Data
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History
  • Received：2025-10-09
Funding
Affiliations
    1.Guangzhou Institute of Food Inspection, Guangzhou 511405, China
    2.School of Life and Health Technology,Dongguan University of Technology, Dongguan 523808, China
    3.Guangdong Dayuan Oasis Food Safety Technology Co., Ltd., Guangzhou 510700, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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