Full
Outline
To establish a method for determination of the content of novel illegal additive dicyclohexylformylphentine in food by high performance liquid chromatography.
Acetonitrile was used as the extraction solvent, and the extraction solution was separated with a Waters Xbridge C18 (250 mm×4.6 mm, 5 μm) chromatographic column. Isocratic elution was carried out with acetonitrile+water as the mobile phase, and quantified by the external standard method. The flow rate was 1.0 mL/min and the detection wavelength was 230 nm.
The dicyclohexylformylphentine had a good linear relationship within the mass concentration range of 0.5–50.0 μg/mL （r2>0.9999）. The limit of detection and limit of quantitation were 1 mg/kg and 5 mg/kg，respectively.At 3 quality control levels of 1 times，2 times and 10 times the limit of quantitation in 3 kinds of different matrices（plums，solid beverages and pressed candies），the recoveries ranged from 85.6% to 99.2%，with relative standard deviations less than or equal to 3.54%. This method was used to evaluate the illegal addition content of a batch of positive samples collected from online platforms，and the results were highly consistent with those determined by high performance liquid chromatography-mass spectrometry.
This method is simple，efficient and accurate，and is suitable for the rapid inspection of illegal additions of dicyclohexylformylphentine in food.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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