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Enzyme-linked immunosorbent assay for detecting soybean agglutinin associated with kidney bean poisoning
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Feng CHEN1, En-De ZHONG2, Xiao-Tian MING1, Ying-Qian ZHANG1, *, Jing-Jing ZHANG3
Journal of Food Safety & Quality | 2025, 16(10) : 186 - 191
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Journal of Food Safety & Quality | 2025, 16(10): 186-191
• Special Topic: Analysis and Monitoring of Toxic and Harmful Substances in Food •
Enzyme-linked immunosorbent assay for detecting soybean agglutinin associated with kidney bean poisoning
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Feng CHEN1, En-De ZHONG2, Xiao-Tian MING1, Ying-Qian ZHANG1, *, Jing-Jing ZHANG3
Affiliations
  • 1. Nantong Center for Disease Control and Prevention, Nantong 226007, China
  • 2. Qingdao Center for Disease Control and Prevention, Qingdao 266071, China
  • 3. Jiangsu Provincial Center for Disease Control and Prevention, Nanjing 210009, China
Published: 2025-05-25 doi: 10.19812/j.cnki.jfsq11-5956/ts.20250226001
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Objective To establish an enzyme-linked immunosorbent assay method for detecting soybean agglutinin in legume foods. Methods After washed the sample, added phosphate buffer solution and homogenize it thoroughly. After centrifugation and purification, collected the supernatant and determine it by enzyme-linked immunosorbent assay. Quantify it used an enzyme-linked immunosorbent assay reader. Result In the linear range of 0-400 μg/mL, the linear equation of soybean agglutinin obtained was Y=0.0021X+0.0528, r=0.9998. The detection limit of this method was 125 mg/kg, the recovery rate was between 99.17% and 101.20%, and the relative standard deviation was between 1.27%-2.24%. Conclusion This method is easy to operate, has good specificity, and the antigen concentration is directly proportional to the OD450 value. Applicable to the detection of soybean agglutinin poisoning incidents by grassroots disease control centers.

enzyme-linked immunosorbent assay method  /  kidney bean  /  poisoning  /  soybean agglutinin
Feng CHEN, En-De ZHONG, Xiao-Tian MING, Ying-Qian ZHANG, Jing-Jing ZHANG. Enzyme-linked immunosorbent assay for detecting soybean agglutinin associated with kidney bean poisoning[J]. Journal of Food Safety & Quality, 2025 , 16 (10) : 186 -191 . DOI: 10.19812/j.cnki.jfsq11-5956/ts.20250226001
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Year 2025 volume 16 Issue 10
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Article Info
doi: 10.19812/j.cnki.jfsq11-5956/ts.20250226001
  • Receive Date：2025-02-26
  • Online Date：2025-07-15
  • Published：2025-05-25
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  • Received：2025-02-26
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Affiliations
    1. Nantong Center for Disease Control and Prevention, Nantong 226007, China
    2. Qingdao Center for Disease Control and Prevention, Qingdao 266071, China
    3. Jiangsu Provincial Center for Disease Control and Prevention, Nanjing 210009, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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