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Objective To establish a method for simultaneous determination of 23 kinds of per-and polyfluoroalkyl substances (PFASs) in food by high efficiency reinforced fat removal bilayer column-ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Methods The sample was extracted and centrifuged, high efficiency reinforced fat removal bilayer column method was adopted for the purification, the sample solution was separated on an Shim-pack GISS Hp 3 μm C18 column using 2 mmol/L ammonium formate and methanol as mobile phase for gradient elution, detected by tandem mass spectrometry with multiple reactions monitoring (MRM), and quantified by internal standard method. Results The 23 kinds of target compounds of PFASs were well retained on the chromatographic column with good linearity in the range of 0.2-50.0 µg/L (r2>0.995). The limits of detection (LODs) and the limits of quantitation (LOQs) about perfluorobutanoic acid (PFBA) and perfluoropentanoic acid (PFPeA) were 0.06 μg/kg and 0.18 μg/kg respectively. LODs and LOQs about the remaining 21 kinds of perfluorinated compounds were 0.03 μg/kg and 0.09 μg/kg, respectively. The recoveries of the 23 kinds of target compounds of 2.0 μg/kg and 10.0 μg/kg spiked levels in the meat, fish and milk matrix ranged from 70.3%-120.0% with relative standard deviations of 1.56%-9.65%. Conclusion This method is easy, fast, accurate and sensitive, which is suitable for the determination of 23 kinds of PFASs in food.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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