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Preparation of edible flavorings using Maillard reaction by combining acid keratin hydrolysate with caramelized feed solution
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Lei GUO1, Li-Peng LI2, Dong-Yue ZONG3, Fu-Shen WANG2, Ji-Bao CAI1, Da XU1, Yao-Wei TIAN1, Li-Li QU2, Chun-Ping XU2, *
Journal of Food Safety & Quality | 2025, 16(10) : 61 - 71
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Journal of Food Safety & Quality | 2025, 16(10): 61-71
Special Topic: Research of Food Flavor
Preparation of edible flavorings using Maillard reaction by combining acid keratin hydrolysate with caramelized feed solution
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Lei GUO1, Li-Peng LI2, Dong-Yue ZONG3, Fu-Shen WANG2, Ji-Bao CAI1, Da XU1, Yao-Wei TIAN1, Li-Li QU2, Chun-Ping XU2, *
Affiliations
  • 1. Research Center, Jiangxi Tobacco Industry Co., Ltd., Nanchang 330096, China
  • 2. College of Tobacco Science and Engineering, Zhengzhou University of Light Industry, Zhengzhou 450000, China
  • 3. Research Center, Jiangsu Tobacco Industry Co., Ltd., Nanjing 210019, China
Published: 2025-05-25 doi: 10.19812/j.cnki.jfsq11-5956/ts.20250207007
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Objective To develop Maillard reaction flavors combined with caramelized syrup using natural plants as raw materials. Methods Using tamarind as a raw material, it was treated with composite enzymes and mixed with caramelized syrup for Maillard reaction. The optimal preparation process was determined according to the comprehensive sensory score standard for Maillard flavors. The aroma differences between the Maillard reaction flavors with and without caramelized syrup were analyzed, and volatile aroma compounds were detected using headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS). The reasons for the aroma differences were analyzed, and its antioxidant activity was studied using 1,1-diphenyl-2-picrylhydrazyl (DPPH). Results The optimal conditions for tamarind enzymatic hydrolysis were: Temperature 45 ℃, time 4.0 hours, and a composite enzyme ratio of 1:1:1 (m:m:m) for pectinase, cellulase, and papain, with an enzyme dosage of 0.5% of the tamarind amount. At a temperature of 120 ℃, reaction time of 80 minutes, initial pH 8.0, and a material-to-liquid ratio of 5:1 (m:V), the Maillard reaction flavor mixed with caramelized syrup showed the highest sensory score. Under these conditions, 68 volatile compounds were detected in the flavor, with ester, heterocyclic, and ketone volatile compounds accounting for a higher proportion compared to the Maillard flavor without caramelized syrup, imparting a sweet-caramel, roasted-sweet, and rich aroma. Adding caramelized syrup enhanced the antioxidant activity of the flavor, and at a concentration of 10 mg/mL, the DPPH scavenging rate reached 70.8%. Conclusion This study provides a new method for preparing edible flavors through the Maillard reaction with tamarind as the raw material combined with caramelized syrup.

acid horn  /  Maillard reaction  /  headspace solid-phase microextraction-gas chromatography-mass spectrometry  /  antioxidant activity
Lei GUO, Li-Peng LI, Dong-Yue ZONG, Fu-Shen WANG, Ji-Bao CAI, Da XU, Yao-Wei TIAN, Li-Li QU, Chun-Ping XU. Preparation of edible flavorings using Maillard reaction by combining acid keratin hydrolysate with caramelized feed solution[J]. Journal of Food Safety & Quality, 2025 , 16 (10) : 61 -71 . DOI: 10.19812/j.cnki.jfsq11-5956/ts.20250207007
Year 2025 volume 16 Issue 10
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Article Info
doi: 10.19812/j.cnki.jfsq11-5956/ts.20250207007
  • Receive Date:2025-02-07
  • Online Date:2025-07-15
  • Published:2025-05-25
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History
  • Received:2025-02-07
Funding
Affiliations
    1. Research Center, Jiangxi Tobacco Industry Co., Ltd., Nanchang 330096, China
    2. College of Tobacco Science and Engineering, Zhengzhou University of Light Industry, Zhengzhou 450000, China
    3. Research Center, Jiangsu Tobacco Industry Co., Ltd., Nanjing 210019, China
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表12种不同金属材料的力学参数

Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)

Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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