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Objective To establish an analytical method for the rapid determination of 5 kinds of peptide toxins in samples from wild mushroom poisoning incidents by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Methods The remaining mushrooms and patient urine samples were extracted by methanol, purified by 250 mg N-propyl ethylenediamine (PSA), and separated by Waters CORTECS C18+ liquid chromatography column. The mobile phase consisted of methanol and 2 mmol/L ammonium acetate (containing 0.1% formic acid) under gradient elution. They were determined by HPLC-MS/MS. Results The 5 kinds of peptide toxins had a good linear relationship (r>0.997) at the concentration 10.0-500.0 µg/L. The limits of detection (LODs) of 5 kinds of peptide toxins in the mushroom samples were 2.0-3.0 µg/kg, the limits of quantification (LOQs) were 5.0-10.0 µg/kg. In the urine samples, the LODs of 5 kinds of peptide toxins were 0.8-1.2 µg/L, the LOQs were 2.0-4.0 µg/L. The recoveries of method were 60.1%-94.9%, and the relative standard deviations were 2.0%-17.7% (n=6). Conclusion This method is accurate and rapid, and suitable for determination of peptide toxins in residual mushroom and patient urine samples from wild mushroom poisoning incidents.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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