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Objective To develop a highly sensitive detection method suitable for accurate qualitative and quantitative analysis of monosaccharide composition in edible fungi polysaccharides. Methods Four kinds of edible fungus polysaccharides samples: Lentinus edodes, Pleurotus eryngii, Hypsizygus marmoreus and Auricularia auricula were selected, after hydrolyzing with trifluoroacetic acid, and deriving with acetic anhydride, the sample was analyzed by gas chromatography-mass spectrometry (GC-MS/SIM). The specificity, precision, linear range, limits of detection (LOD) and limits of quantification (LOQ) of this method were investigated with mixed standards of 7 kinds of monosaccharides. At different m/z, the best linear relationship was used as the standard to select the most suitable quantitative ion for each monosaccharide. The stabilities and recoveries of the method under selected ion monitoring (SIM) mode were investigated using edible fungus polysaccharide samples. Results The results showed that m/z 115, m/z 103, m/z 115, m/z 115, m/z 115, m/z 115 and m/z 103 were selected as the characteristic quantitative ions of the corresponding derivatives of ribose, rhamnose, arabinose, xylose, mannose, glucose and galactose, respectively. The separation of each monosaccharide was greater than 1.2, and the linear relationship between each monosaccharide in SIM mode was good between 10.00-50.00 μg/mL, and the correlation coefficient (r2) was greater than 0.998. The LOD and LOQ were 5.75-22.23 ng/mL and 17.26-66.67 ng/mL, respectively. Using monosaccharide mixed standard and lentinan as sample matrix, it was proved that this method had good precision and stability, and the average recovery of each monosaccharide was between 94.4% and 101.3% under different standard adding levels. And the rationality of selecting the above characteristic ions was discussed by mass spectrometry analysis. Conclusion This method has good separation, precision and stability, and its sensitivity is higher than most similar detection methods reported so far. It is suitable for the detection of monosaccharide composition in various edible fungi polysaccharides.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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