Light spectrum regulation is an effective way to promote carbon fixation of haematococcus pluvialis. The carbon fixation capacity of haematococcus pluvialis was compared under different light conditions, and the effects of the mixed red and blue light with different spectral ratios on the carbon fixation performance of haematococcus pluvialis were studied. Gene expressions at different vegetative stages were compared to figure out the dynamic changes of haematococcus pluvialis metabolism on the time scale. The results show that, the carbon fixation rate in red light was 40% higher than that in blue light. By comparing the whole-genome transcriptome of the haematococcus pluvialis, the metabolic responses of the haematococcus pluvialis to different light qualities, including biomass accumulation, energy transfer, photosynthesis, stress and transcription, were analyzed at the molecular level. The metabolism and energy transfer of the haematococcus pluvialis were more active in red light than that in blue light. At the early growth stage, cells under the red light dominant condition tended to accumulate regulatory substances rather than energy storage substances, and genes involved in glycolysis/ gluconeogenesis pathway, tricarboxylic acid cycle (TCA) and AMP-activated protein kinase (AMPK) pathway were up-regulated. Cells under the blue light dominant condition performed better in stress response.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |