In the era of new media, short videos has grown into one of the popular video forms among Chinese netizens. Popular science short videos, as an important branch of short videos, play a pivotal role in the popularization of science, eliminating the barriers of time and space in knowledge dissemination. Taking "Zhang Chenliang's popular science daily" account as the research subject and utilizing content analysis, this study reveals the techniques and Tao of creating popular science short videos in the field of life sciences. The techniques mainly include the popular science themes, release time, types of titles, presence of advertisements, language style, and keeping up with current trends. The Tao refers to scientific spirit, mission commitment, cultural cultivation, and educational sentiment. By extracting the key elements possessed by excellent popular science short videos, this study aims to provide valuable insights for content producers in the field of popular science short videos.