Objective To investigate the relationship of longitudinal trajectory of systolic blood pressure (SBP) to new-onset chronic kidney disease (CKD). Methods A prospective cohort study was performed with a total of 13 985 on job and retired Kailuan Group employees who met the standard and participated in health examination. SAS Proc Traj program was used to identify the longitudinal trajectories of SBP of the first three times health examination, and 5 different SBP trajectory groups formed: low-stability trajectory group (106.42-109.43 mmHg, n=2927), medium-stability trajectory group (120.63-124.46 mmHg, n=6916), medium-high trajectory group (132.22-142.97 mmHg, n=2716), high-medium trajectory group (156.77-134.15 mmHg, n=668) and high-stability trajectory group (154.65-158.98 mmHg, n=758). Log-rank test was performed to compare the differences of new-onset CKD cumulative incidence among different SBP trajectory groups during follow-up; Multi-Cox regression model was used to analyze the effect of different SBP longitudinal trajectories on new-onset CKD. Results The mean age of 13 985 subjects was (49.5±11.0)years, including 10 712 males (76.6%) and 3273 females (23.4%), the mean follow-up time was (6.8±1.0) years. The cumulative incidence of CKD was 13.9% (1187/13 985). The cumulative incidences of CKD in low-stability trajectory group, medium-stability trajectory group, medium-high trajectory group, high-medium trajectory group and high-stability trajectory group were 7.7% (147/2927), 12.2% (514/6916), 17.8% (291/2716), 21.9% (93/668), and 35.4% (142/758), respectively, the incidence of CKD increased with elevated stability trajectory (log-rank χ²=177.561, P<0.001). Multi-Cox regression model analysis showed that with adjusted confounding factors, compared with the subjects in low-stability trajectory group, SBP longitudinal trajectories were the risk factors for new-onset CKD in medium-high trajectory group, high-medium trajectory group and high-stability trajectory group, and the HR and 95%CI were 1.36 (1.04-1.77), 1.62 (1.08-2.43) and 2.06 (1.40-3.02), respectively. And the influence of SBP longitudinal trajectory on new-onset CKD was better than that of baseline SBP level. Conclusion Elevated longitudinal trajectory of SBP is the risk factor for new-onset CKD in Kailuan group population.

Diabetic retinopathy and diabetic nephropathy are two chronic microvascular complications of diabetes. Some studies have shown that the both microvascular complications possess similar pathological mechanisms and some differences. The gold standard for clinical diagnosis of diabetic nephropathy is the pathology of renal biopsy. Of which, however, the acceptance of patients and the popularity of the technology have been limited for its invasiveness and technical difficulty. Therefore, the key to solve this problem is to seek a reliable noninvasive diagnosis method. The diabetic retinopathy diagnosed by color fundus photography opens the possibility of noninvasive diagnosis of diabetic nephropathy. A growing number of studies have shown that it has a high degree of credibility and feasibility to combine diabetic retinopathy and other clinical indicators to assist in diagnosing diabetic nephropathy. The effects and diagnostic value of diabetic retinopathy on diabetic nephropathy, therefore, have been reviewed in present article.

Objective To evaluate the efficiency and safety of single ProGlide pre-suture technique for hemostasis of vein puncture point after removal of the sheath tube in patients undergoing percutaneous intervention with large-caliber femoral vein sheath. Methods A total of 26 patients were enrolled in present retrospective analysis, including 18 patients receiving extracorporeal membrane oxygenation and 8 patients undergoing radiofrequency ablation operation for arrhythmia. Clinical evaluation and vascular ultrasound examination were performed for all the subjects after operation to observe, record and evaluate the success rate of single ProGlide pre-suture technique and the incidence of complications at venipuncture points during hospitalization. Results Twenty males and 6 females with average age of (66.5±11.7) years were recruited in present study, including 10 cases of hypertension (38.5%),7 cases of diabetes (26.9%), 4 cases of chronic renal insufficiency (15.4%), 2 cases of chronic obstructive pulmonary disease (7.7%)and 17 cases of peripheral arterial disease (65.4%). The achievement ratio of single ProGlide pre-suture technique was 96.2%, only one patient required hemostasis by manual compression after removal of the sheath and suture. No complications related to venipuncture occurred during hospitalization period. Conclusion In percutaneous intervention, rapid, safe and effective hemostasis of vein puncture point can be achieved with single ProGlide pre-suture technique after removal of the large-caliber vein sheath.

Objective To investigate the effects of silencing axon guide protein 4D (Sema4D) on the growth, autophagy and epithelial-mesenchymal transformation of gastric cancer cell SGC-7901. Methods The cells were randomly divided into control group, shRNA-NC group, Sema4D-shRNA1 group, Sema4D-shRNA2 group and Sema4D-shRNA3 group, and transfected into SGC-7901 with shRNA Sema4D vector. The expression of Sema4D mRNA was detected by RT-PCR. The clone formation rate was determined by colony formation assay. Flow cytometry was used to detect apoptosis. Morphological changes of epithelial-mesenchymal transformation were observed microscopically. Western blotting was used to detect the expression levels of Ki67, proliferating cell nuclear antigen (PCNA), Bax, Bcl-2, caspase-3, cleaved caspase-3, Beclin1, p62, LC3Ⅰ, LC3Ⅱ, E-cadherin, N-cadherin, and vimentin proteins. Results The results of RT-PCR showed that compared with the control group, the mRNA levels of Sema4D-shRNA1 group, Sema4D-shRNA2 group and Sema4D-shRNA3 group were significantly lower, and the mRNA level of Sema4D-shRNA3 group was significantly lower than that of Sema4D-shRNA1 group and Sema4D-shRNA2 group (P<0.05).Sema4D-shRNA3 was selected for subsequent experiments. The results of colony forming assay showed that the colony forming efficiency of Sema4D-shRNA3 group was significantly lower than that of the control group (P<0.05). Flow cytometry results showed that compared with the control group, the apoptosis rate of Sema4D-shRNA3 group was significantly increased (P<0.05).Western blotting results showed that compared with the control group, the expression levels of Ki67, PCNA, p62, N-cadherin and vimentin proteins in the Sema4D-shRNA3 group were significantly lower, Beclin1 and E-cadherin proteins were significantly higher, and the ratios of Bax/Bcl-2, cleaved caspase-3/caspase-3 and LC3Ⅱ/LC3Ⅰ were significantly higher (P<0.05). Microscopically, the epithelial-mesenchymal transformation was inhibited in the Sema4D-shRNA3 group. Conclusion shRNA Sema4D can inhibit proliferation, autophagy and epithelial-mesenchymal transformation of SGC-7901 cells and promote apoptosis.