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Tissue clearing is a fast-developing new histological technology. It treats large tissues, single organs, the whole body of rodents and large human specimens through a series of physicochemical principles and methods to rapidly achieve a high degree of optical transparency while maintaining their integrity, providing an important tool for obtaining three-dimensional structural information of biological tissues with high resolution. Combining the modern optical imaging and fluorescent labeling technology can significantly improve the imaging depth and image contrast, and help accelerate the process of acquiring fine structural and molecular function information such as neurons, synapses, and heterogeneous pathological components throughout the brain. It has become a promising alternative to classical histological techniques and is widely used in life science fields. The main methodological principles, advantages and disadvantages of brain tissue clearing are reviewed in present paper, and combs the application of tissue clearing in neurodegenerative diseases, observes the degenerative changes of fine structures such as neurons, axons and myelin at a holistic level, and the evolution of heterogeneous pathological components between cells at a whole brain scale, so for providing the possibility to eventually turn to the study of human neurodegenerative brain tissues.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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