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Study on the Protective Effect and Mechanism of Anmei Dan on Astrocyte and Neuron Oxygen-Glucose Deprivation/Reoxygenation Injury Based on the EphA4/ephrinA3 Signaling Pathway
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Yixuan WU1, Yaqi LI1, Zhujiang LI1, Jiecheng JIANG1, Zhuo ZHANG1, Ping WANG1, Guangjing XIE1, 2, *, Panpan HUANG1, 2
Acta Chinese Medicine and Pharmacology | 2026, 54(10) : 13 - 20
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Acta Chinese Medicine and Pharmacology | 2026, 54(10): 13-20
Study on the Protective Effect and Mechanism of Anmei Dan on Astrocyte and Neuron Oxygen-Glucose Deprivation/Reoxygenation Injury Based on the EphA4/ephrinA3 Signaling Pathway
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Yixuan WU1, Yaqi LI1, Zhujiang LI1, Jiecheng JIANG1, Zhuo ZHANG1, Ping WANG1, Guangjing XIE1, 2, *, Panpan HUANG1, 2
Affiliations
  • 1.Engineering Research Center of Ministry of Education for Traditional Chinese Medicine Prevention and Protection Technologies and New Product Development for Senile Brain Health, Hubei University of Chinese Medicine, Wuhan 430065, China
  • 2.Hubei Shizhen Laboratory, Wuhan 430065, China
Published: 2026-10-20 doi: 10.19664/j.cnki.1002-2392.260196
Outline
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Objective:

To investigate the protective effects and underlying mechanisms of Anmeidan (AMD) against oxygen-glucose deprivation/reoxygenation (OGD/R)-induced injury in astrocytes and neurons by regulating the EphA4/ephrinA3 signaling pathway.

Methods:

An invitro OGD/R model was established using mouse astrocytes (C8-D1A) and hippocampal neurons (HT22 cells). Control serum and AMD-containing serum were prepared. Cells were divided into control, OGD/R, OGD/R+AMD, and OGD/R+melatonin groups. Cell proliferation and viability were assessed via CCK-8 assay to determine the optimal AMD serum concentration. Apoptosis rates were measured by flow cytometry. Immunofluorescence staining was performed to evaluate fluorescence intensity of neuronal marker NeuN, astrocytic marker GFAP, and neurogenesis marker BrdU. Protein expression of ephrinA3, EphA4, EAAT1, EAAT2, and SYN was analyzed by Western blot. Cellular ultrastructure was observed via transmission electron microscopy (TEM).

Results:

Compared with the control group, the OGD/R group showed reduced fluorescence intensity of NeuN and GFAP (P<0.01), abnormal neuronal morphology, upregulated EphA4/ephrinA3 protein expression (P<0.01), and decreased EAAT1/EAAT2 levels (P<0.05, P<0.01). AMD treatment significantly enhanced GFAP and NeuN fluorescence intensity, improved neuronal morphology, downregulated EphA4/ephrinA3 expression (P<0.01), upregulated EAAT1, EAAT2, SYN (P<0.05), and reduced apoptosis rates (P<0.01).

Conclusion:

Anmeidan alleviates OGD/R-induced astrocyte and neuronal injury by downregulating the EphA4/ephrinA3 signaling pathway and modulating glutamate transport, which may underlie its therapeutic potential in sleep deprivation-related neural damage.

Sleep deprivation  /  Oxygen-glucose deprivation/reoxygenation  /  Anmei Dan  /  Astrocytes  /  Tyrosine kinase adrenergic receptor 4/tyrosine protein kinase A3 receptor signaling pathway
吴艺璇, 李娅琪, 黎柱江, 姜杰程, 张卓, 王平, 谢光璟, 黄攀攀. 基于EphA4/ephrinA3信号通路研究安寐丹对星形胶质细胞和神经元氧糖剥夺/复氧损伤的保护作用及机制. 中医药学报, 2026 , 54 (10) : 13 -20 . DOI: 10.19664/j.cnki.1002-2392.260196
Yixuan WU, Yaqi LI, Zhujiang LI, Jiecheng JIANG, Zhuo ZHANG, Ping WANG, Guangjing XIE, Panpan HUANG. Study on the Protective Effect and Mechanism of Anmei Dan on Astrocyte and Neuron Oxygen-Glucose Deprivation/Reoxygenation Injury Based on the EphA4/ephrinA3 Signaling Pathway[J]. Acta Chinese Medicine and Pharmacology, 2026 , 54 (10) : 13 -20 . DOI: 10.19664/j.cnki.1002-2392.260196
Appendix
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Year 2026 volume 54 Issue 10
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Article Info
doi: 10.19664/j.cnki.1002-2392.260196
  • Receive Date：2025-07-10
  • Online Date：2026-10-14
  • Published：2026-10-20
Article Data
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History
  • Received：2025-07-10
  • Revised：2026-05-11
Funding
Affiliations
    1.Engineering Research Center of Ministry of Education for Traditional Chinese Medicine Prevention and Protection Technologies and New Product Development for Senile Brain Health, Hubei University of Chinese Medicine, Wuhan 430065, China
    2.Hubei Shizhen Laboratory, Wuhan 430065, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
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鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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