PDF
Full
Outline
To observe the effect of Bear bile and UDCA on circulating blood Ly6Chigh monocyte chemotaxis receptor CCR2 and its ligand CCL2 (MCP-1) in model mice with Immune Thrombocytopenia (ITP), and explore the possible mechanism of regulating Ly6Chigh monocyte migration and reference studies on bear bile and its replacements in ITP management.
Following established methods, an ITP model was induced via immunization in 50 BALB/c mice, randomLy allocated into five groups: normal control, model control, prednisone treatment, bear bile treatment, and UDCA treatment, from the 6th day of making model, mice in the each group was administered 0.4 mL/d volume of corresponding drug by irrigation treatment. From the 6 h, 7th day, 15th day, automatic blood cytometer was used to dynamic detect platelet count. after treatment 10 day, Flow cytometry was used to detect the changes of the Ly6Chigh monocyte in circulating blood, Enzyme-linked immunosorbent Assay was used to detect the levels of serum MCP-1 (monocyte chemotactic protein-1).
After the 6 hour of making model, the counts of platelet decreased obviously in all group, reaching the lowest point on the 7th day, and remained at a relatively low level until the 15th day. There was a statistically significant difference compared to the normal group (P < 0.05). After treatment, the platelet counts of bear bile, prednisone and UDCA group increased obviously (P < 0.01). Flow cytometry results showed that compared with the model group, the positive rate of circulating Ly6Chigh monocytes and its surface chemokine receptor CCR2 in bear bile, UDCA and prednesone groups decreased (P < 0.01, P < 0.001), while CX3CR1 expression increased (P < 0.001). Enzyme-linked immunosorbent Assay results showed that compared with the model group, the levels of MCP-1 in bear bile, UDCA and prednesone groups decreased(P < 0.001).
The Bear bile and UDCA can downregulate the expression of CCR2 by reducing the level of MCP-1 in circulating blood, thus governs CCL2-CCR2 mediated Ly6Chigh monocyte emigration, reduce the Ly6Chigh monocytes in the circulating blood, this represents a crucial mechanism driving Ly6Chigh monocyte accumulation during ITP progression and worsening.
PDF
5
2
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62199257
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
