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To investigate the effect of three different strains on the chemical composition of Polygonatum in the fermentation process of Polygonatum.
the crushed Rhizoma Polygonati was prepared into a suspension according to the 30 ∶ 70 ratio of care solution, and the three activated bacterial solutions of Lactobacillus casei, Lactobacillus rhamnosus and Bacillus subtilis were separately added according to the 5% inoculum. The sample morphology and the number of viable bacteria were observed. Ultra performance liquid chromatography quadrupole time-of-flight mass spectrometry (uplc-q-tof-ms) was used to analyze the differential chemical components of Polygonatum sibiricum before and after fermentation.
Rhizoma Polygonati was thickened after fermentation, with fluidity and sour taste. Milky white folds appeared after fermentation by Lactobacillus rhamnosus and Bacillus subtilis. On the 6th day of fermentation, Lactobacillus casei and Bacillus subtilis had the highest viable counts, which were 3.40 × 108 CFU/g and 2.62 × 109 CFU/g, respectively; The number of viable bacteria was the highest when Lactobacillus rhamnosus was fermented for 7 days, which was 2.59 × 108 CFU/g; Three triterpenoid saponins, 2 flavonoids, 1 organic acid.
Lactobacillus rhamnosus and Lactobacillus casei can convert triterpenoid steroidal saponins into aglycones during the fermentation of Polygonatum sibiricum, and the utilization effect of saponins is better. Bacillus subtilis can convert flavonoid glycosides into aglycones, and the utilization effect of flavonoid glycosides is better.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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