Objective:To investigate the therapeutic effects and mechanism of Diyu Qingbi Ointment on imiquimod cream（IMQ）-in duced psoriasis model mice.Methods:Fifty BALB/c mice were randomly divided into five groups:blank group，model group，modified Jiawei Qingbi group（treated with modified Diyu Qingbi Ointment），Diyu group（treated with Diyu Qingbi Ointment），and Calcipotriol ointment group.Each group contained 10 mice.The blank group received daily application of Vaseline ointment，while the remaining groups were subjected to daily 5% IMQ application to induce the model，followed 6 hours later by the corresponding drug intervention，continuously for 7 days.On day 7，psoriasis lesion area and severity index（PASI）scores were recorded；after the intervention，mice were sacrificed，psoriatic lesions were collected for HE staining to observe pathological morphological changes.ELISA was used to detect serum expression levels of interleukin-6（IL-6），interleukin-22（IL-22），and tumor necrosis factor-alpha（TNF-α）.qPCR was used to detect mRNA expression levels of Janus kinase 1（JAK1）and signal transducer and activator of transcription 3（STAT3）in lesion tissues；Western blot analysis was used to detect the expression levels of JAK1，p-STAT3/STAT3，and Survivin proteins in psoriatic lesions.Results:HE staining results showed that mice in the model group exhibited erythema，scaling，infiltrative lesions，as well as abnormal keratinization，hyperkeratosis，and Munro microabscesses，indicating psoriasis-like pathological changes. Compared with the model group，the pathological manifestations of each treatment group were alleviated to varying degrees，and PASI scores were significantly reduced，with the modified formula group showing lower PASI scores than the calcipotriol group（P＜0.05）.ELISA results indicated that compared with the blank group，the model group had significantly elevated levels of IL-6，IL-22，and TNF-α（P＜0.05）；compared with the model group，levels of IL-6，IL-22，and TNF-αin each treatment group were significantly reduced（P＜0.05）；IL-6 levels in the modified formula and Sanguisorba officinalis groups were significantly lower than those in the calcipotriol group（P＜0.05），and IL-22 levels in the modified formula group were significantly lower than those in both the Sanguisorba officinalis and calcipotriol groups（P＜0.05）.qPCR and Western blot results showed that compared with the blank group，the model group had significantly higher protein expression levels of JAK1，p-STAT3/STAT3，and Survivin，as well as elevated mRNA levels of JAK1 and STAT3（P＜0.05）；compared with the model group，expression levels of JAK1，p-STAT3/STAT3，Survivin proteins，and JAK1 and STAT3 mRNA were significantly reduced in the treatment groups（P＜0.05）；p-STAT3/STAT3 in the modified formula group was significantly lower than in the Sanguisorba officinalis group（P＜0.05），and JAK1 mRNA expression in the modified formula group was significantly lower than in the calcipotriol group（P＜0.05）. Conclusion:Modified Diyu Qingbi Ointment can reduce the severity of skin lesions in psoriatic model mice and improve their pathological manifestations，performing overall better than Sanguisorba officinalis Qingbi ointment.Its mechanism may be related to reducing the expression of inflammatory cytokines such as IL-6，IL-22，TNF-α，and inhibiting theJAK1/STAT3 signaling pathway as well as the expression of the apoptosis-inhibiting protein Survivin.