Article(id=1322922779296793256, tenantId=1146029695717560320, journalId=1255847919539208197, issueId=1322922680609014711, articleNumber=null, orderNo=null, doi=10.13193/j.issn.1673-7717.2025.02.025, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=null, receivedDateStr=null, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1793338969300, onlineDateStr=2026-10-30, pubDate=1739116800000, pubDateStr=2025-02-10, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1793338969300, onlineIssueDateStr=2026-10-30, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1793338969300, creator=13701087609, updateTime=1793338969300, updator=13701087609, issue=Issue{id=1322922680609014711, tenantId=1146029695717560320, journalId=1255847919539208197, year='2025', volume='43', issue='2', pageStart='1', pageEnd='258', issueExtLink='null', onlineDate='null', pubDate='1739116800000', pubDateStr='2025-02-10', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=0, createTime=1793338945772, creator='13701087609', updateTime=1793340618065, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1322929694785536732, tenantId=1146029695717560320, journalId=1255847919539208197, issueId=1322922680609014711, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1322929694785536733, tenantId=1146029695717560320, journalId=1255847919539208197, issueId=1322922680609014711, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=108, endPage=111, ext={EN=ArticleExt(id=1322922779493925545, articleId=1322922779296793256, tenantId=1146029695717560320, journalId=1255847919539208197, language=EN, title=Rapid Screening of Antioxidants of Oil Extracted from Bangmaoluoxinfu(Trichonephila clavata)Based on GC-MS Coupled with DPPH Assay, columnId=1256183361521967297, journalTitle=Chinese Archives of Traditional Chinese Medicine, columnName=Target on National Project, runingTitle=null, highlight=null, articleAbstract=
Objective

To establish a gas chromatography mass spectrometry(GC-MS)technique using 1,1-Diphenyl-2-picrylhydrazyl(DPPH)as a free radical for rapid screening of antioxidant active components in the oil extracted from Bangmaoluoxinfu(Trichonephila clavata).

Methods

The method combining GC-MSchromatographic analysis with DPPH radical chemical reaction was used to screen antioxidant components by analyzing the changes in the chromatographic of the oil extracted from Bangmaoluoxinfu(Trichonephila clavata)before and after reaction with DPPH. Furthermore,the antioxidant activity of each compound was evaluated by the rate of reduction of the peak area of the chromatograms. At the same time,to verify the screening results of antioxidants by the DPPH-GC-MSmethod,a methodological study was carried out,and the free radical scavenging ability of the control product was determined by a microplate reader.

Result

A total of 22 compounds were identified from the oil extracted from Bangmaoluoxinfu(Trichonephila clavata),accounting for 89.67% of the total,mainly consisting of fatty acids and esters,with relatively high levels of methyl oleate and methyl linoleate. Sixteen antioxidants were rapidly screened from them by the DPPH-GC-MSmethod,among which 2,4-Di-tert-butylphenol,ethyl stearate,linoleic acid,ethyl linoleate,ethyl oleate and palmitic acid ethyl ester showed strong antioxidant capacity. The results of activity verification showed that the DPPH-GC-MS method was reliable and can be used for rapid screening and identification of antioxidants in natural products.

Conclusion

The DPPH-GC-MSmethod can simply and efficiently screen out the antioxidants in the oil extracted from Bangmaoluoxinfu(Trichonephila clavata),and provided the experimental basis for further development and utilization of Bangmaoluoxinfu(Trichonephila clavata)resources.

, authors=Shiyu WANG, Heng LYU, Meng GAO, Tongtong CHEN, Chenxi ZHAO, Jianrong LUO, authorsList=Shiyu WANG, Heng LYU, Meng GAO, Tongtong CHEN, Chenxi ZHAO, Jianrong LUO, authorCompany=null, correspAuthors=Jianrong LUO, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1322922780555084466, articleId=1322922779296793256, tenantId=1146029695717560320, journalId=1255847919539208197, language=CN, title=基于DPPH联合GC-MS技术快速筛选棒毛络新妇油脂的抗氧化成分, columnId=1256183362172142382, journalTitle=中华中医药学刊, columnName=国家项目点击, runingTitle=null, highlight=null, articleAbstract=
目的

建立1,1-二苯基-2-三硝基苯肼自由基(1,1-Diphenyl-2-picrylhydrazyl,DPPH)联合气相色谱和质谱联用技术(Gas Chromatography-Mass Spectrometry,GC-MS)快速筛选棒毛络新妇油脂中的抗氧化活性成分。

方法

采用DPPH自由基化学反应与GC-MS色谱分析相结合的方法进行研究,通过分析棒毛络新妇油脂与自由基反应前后色谱图的变化来筛选抗氧化成分,并以各化合物的色谱峰面积降低率评价其抗氧化活性。同时进行方法学考察,并采用酶标仪法测定对照品的自由基清除能力,以验证DPPH-GC-MS方法的抗氧化成分筛选结果。

结果

从棒毛络新妇油脂中共鉴定出22个化合物,占总量的89.67%,主要为脂肪酸和脂肪酸酯,其中油酸甲酯和亚油酸甲酯相对含量较高。通过DPPH-GC-MS方法从中快速筛选到16个抗氧化成分,其中2,4-二叔丁基苯酚、硬脂酸乙酯、亚油酸、亚油酸乙酯、油酸乙酯和棕榈酸乙酯的抗氧化能力较强。活性验证结果表明DPPH-GC-MS方法可靠,可对天然产物中的抗氧化活性物质进行快速筛选和鉴定。

结论

DPPH-GC-MS方法可简便高效地筛选出棒毛络新妇油脂中的抗氧化成分,为进一步开发利用棒毛络新妇资源提供实验依据。

, authors=王诗雨, 吕恒, 高萌, 陈彤彤, 赵晨曦, 罗建蓉, authorsList=王诗雨, 吕恒, 高萌, 陈彤彤, 赵晨曦, 罗建蓉, authorCompany=null, correspAuthors=罗建蓉, authorNote=

王诗雨(1998-),女,黑龙江齐齐哈尔人,硕士在读,研究方向:药效物质基础。

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罗建蓉(1979-),女(傣族),云南玉溪人,副教授,硕士研究生导师,研究方向:药效物质基础及质量标准。E-mail:。
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王诗雨(1998-),女,黑龙江齐齐哈尔人,硕士在读,研究方向:药效物质基础。

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pageStart=555, pageEnd=572, url=null, language=null, rfNumber=[1], rfOrder=0, authorNames=KUNTNER M, HAMILTON C A, CHENG R C, journalName=Syst Biol, refType=null, unstructuredReference=KUNTNER M,HAMILTON C A,CHENG R C,et al. Golden orbweavers ignore biological rules:phylogenomic and comparative analyses unravel a complex evolution of sexual size dimorphism[J]. Syst Biol,2019,68(4):555-572., articleTitle=Golden orbweavers ignore biological rules:phylogenomic and comparative analyses unravel a complex evolution of sexual size dimorphism, refAbstract=null), Reference(id=1322922788230660850, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922779296793256, doi=null, pmid=null, pmcid=null, year=2021, volume=11, issue=1, pageStart=10680, pageEnd=null, url=null, language=null, rfNumber=[2], rfOrder=1, authorNames=YONG H S, SONGSL, CHUA K O, journalName=Sci Rep, refType=null, unstructuredReference=YONG H S,SONGSL,CHUA K O,et al. Complete mitochondrial genomes and phylogenetic relationships of the Genera Nephila and Trichonephila(Araneae,Araneoidea)[J]. Sci Rep,2021,11(1):10680., articleTitle=Complete mitochondrial genomes and phylogenetic relationships of the Genera Nephila and Trichonephila(Araneae,Araneoidea), refAbstract=null), Reference(id=1322922788314546931, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922779296793256, doi=null, pmid=null, pmcid=null, year=2013, volume=28, issue=3, pageStart=56, pageEnd=59, url=null, language=null, rfNumber=[3], rfOrder=2, authorNames=万晓瑜, 廖绍波, journalName=楚雄师范学院学报, refType=null, unstructuredReference=万晓瑜,廖绍波. 云南民间食用蜘蛛初报[J]. 楚雄师范学院学报,2013,28(3):56-59., articleTitle=云南民间食用蜘蛛初报, refAbstract=null), Reference(id=1322922788385850100, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922779296793256, doi=null, pmid=null, pmcid=null, year=2013, volume=null, issue=null, pageStart=432, pageEnd=433, url=null, language=null, rfNumber=[4], rfOrder=3, authorNames=李军德, 黄璐琦, 曲晓波, journalName=中国药用动物志, refType=null, unstructuredReference=李军德,黄璐琦,曲晓波. 中国药用动物志[M]. 2版. 福州:福建科学技术出版社,2013:432-433., articleTitle=null, refAbstract=null), Reference(id=1322922788452958965, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922779296793256, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[5], rfOrder=4, authorNames=高鹏飞, 刘正文, 郑园园, journalName=null, refType=null, unstructuredReference=高鹏飞,刘正文,郑园园,等. 络新妇属蜘蛛提取物抗肿瘤部位的制备方法及医药用途:CN103638057A[P]. 2014-03-19., articleTitle=络新妇属蜘蛛提取物抗肿瘤部位的制备方法及医药用途, refAbstract=null), Reference(id=1322922788536845046, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922779296793256, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[6], rfOrder=5, authorNames=巫秀美, 郭华, 冯萍, journalName=null, refType=null, unstructuredReference=巫秀美,郭华,冯萍,等. 抑制真菌生长的络新妇属蜘蛛有效部位的制备及其用途:CN103655631A[P]. 2014-03-26., articleTitle=抑制真菌生长的络新妇属蜘蛛有效部位的制备及其用途, refAbstract=null), Reference(id=1322922788629119735, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922779296793256, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[7], rfOrder=6, authorNames=陈俊雅, 李启艳, 方春生, journalName=null, refType=null, unstructuredReference=陈俊雅,李启艳,方春生,等. 一种络新妇属蜘蛛有效部位的制备及抗革兰氏菌医药用途:CN103638058A[P]. 2014-03-19., articleTitle=一种络新妇属蜘蛛有效部位的制备及抗革兰氏菌医药用途, refAbstract=null), Reference(id=1322922788692034296, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922779296793256, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[8], rfOrder=7, authorNames=杨自忠, 朱振东, 马艳滟, journalName=null, refType=null, unstructuredReference=杨自忠,朱振东,马艳滟,等. 一种具抗病毒功效的络新妇属蜘蛛有效部位及其制备方法:CN104042632B[P]. 2018-05-01., articleTitle=一种具抗病毒功效的络新妇属蜘蛛有效部位及其制备方法, refAbstract=null), Reference(id=1322922788754948857, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922779296793256, doi=null, pmid=null, pmcid=null, year=2005, volume=1048, issue=1-2, pageStart=170, pageEnd=176, url=null, language=null, rfNumber=[9], rfOrder=8, authorNames=SALAMONI SD, COSTA DA COSTA J, PALMA M S, journalName=Brain Res, refType=null, unstructuredReference=SALAMONI SD,COSTA DA COSTA J,PALMA M S,et al. Antiepileptic effect of acylpolyaminetoxin JSTX-3 on rat hippocampal CA1 neurons in vitro[J]. Brain Res,2005,1048(1-2):170-176., articleTitle=Antiepileptic effect of acylpolyaminetoxin JSTX-3 on rat hippocampal CA1 neurons in vitro, refAbstract=null), Reference(id=1322922788817863418, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922779296793256, doi=null, pmid=null, pmcid=null, year=2017, volume=49, issue=7, pageStart=1237, pageEnd=1245, url=null, language=null, rfNumber=[10], rfOrder=9, authorNames=JIN L, FANG M Q, CHEN M R, journalName=Amino Acids, refType=null, unstructuredReference=JIN L,FANG M Q,CHEN M R,et al. An insecticidal toxin from Nephila clavata spider venom[J]. Amino Acids,2017,49(7):1237-1245., articleTitle=An insecticidal toxin from Nephila clavata spider venom, refAbstract=null), Reference(id=1322922788889166587, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922779296793256, doi=null, pmid=null, pmcid=null, year=2021, volume=11, issue=4, pageStart=563, pageEnd=null, url=null, language=null, rfNumber=[11], rfOrder=10, authorNames=FUJIWARA M, KONO N, HIRAYAMA A, journalName=Biomolecules, refType=null, unstructuredReference=FUJIWARA M,KONO N,HIRAYAMA A,et al. Xanthurenic acid is the main pigment of Trichonephila clavata gold dragline silk[J]. Biomolecules,2021,11(4):563., articleTitle=Xanthurenic acid is the main pigment of Trichonephila clavata gold dragline silk, refAbstract=null), Reference(id=1322922788977246972, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922779296793256, doi=null, pmid=null, pmcid=null, year=2018, volume=30, issue=11, pageStart=1913, pageEnd=1917, url=null, language=null, rfNumber=[12], rfOrder=11, authorNames=施树云, 郭柯柯, 彭胜, journalName=天然产物研究与开发, refType=null, unstructuredReference=施树云,郭柯柯,彭胜,等. DPPH-HPLC-QTOF-MS/MS快速筛选和鉴定杜仲黑茶中抗氧化活性成分[J]. 天然产物研究与开发,2018,30(11):1913-1917., articleTitle=DPPH-HPLC-QTOF-MS/MS快速筛选和鉴定杜仲黑茶中抗氧化活性成分, refAbstract=null), Reference(id=1322922789065327357, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922779296793256, doi=null, pmid=null, pmcid=null, year=2021, volume=24, issue=8, pageStart=550, pageEnd=552,557, url=null, language=null, rfNumber=[13], rfOrder=12, authorNames=袁永雷, 王飞飞, 曲丽萍, journalName=中国药师, refType=null, unstructuredReference=袁永雷,王飞飞,曲丽萍. HPLC-DPPH快速发现重楼果壳总黄酮中抗氧化成分及其活性初步验证[J]. 中国药师,2021,24(8):550-552,557., articleTitle=HPLC-DPPH快速发现重楼果壳总黄酮中抗氧化成分及其活性初步验证, refAbstract=null), Reference(id=1322922789136630526, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922779296793256, doi=null, pmid=null, pmcid=null, year=2022, volume=53, issue=2, pageStart=185, pageEnd=191, url=null, language=null, rfNumber=[14], rfOrder=13, authorNames=钱金珠, 梁明, 余意, journalName=中国药科大学学报, refType=null, unstructuredReference=钱金珠,梁明,余意,等. HPLC-DPPH在线联用技术评价制首乌的抗氧化活性[J]. 中国药科大学学报,2022,53(2):185-191., articleTitle=HPLC-DPPH在线联用技术评价制首乌的抗氧化活性, refAbstract=null), Reference(id=1322922789203739391, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922779296793256, doi=null, pmid=null, pmcid=null, year=2020, volume=9, issue=8, pageStart=702, pageEnd=null, url=null, language=null, rfNumber=[15], rfOrder=14, authorNames=WANG X, ZUO G L, WANG C Y, journalName=Antioxidants, refType=null, unstructuredReference=WANG X,ZUO G L,WANG C Y,et al. An off-line DPPHGC-MScoupling countercurrent chromatography method for screening,identification,and separation of antioxidant compounds in essential oil[J]. Antioxidants,2020,9(8):702., articleTitle=An off-line DPPHGC-MScoupling countercurrent chromatography method for screening,identification,and separation of antioxidant compounds in essential oil, refAbstract=null), Reference(id=1322922789270848256, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922779296793256, doi=null, pmid=null, pmcid=null, year=2020, volume=144, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[16], rfOrder=15, authorNames=NIE JY, LIR, JIANGZ T, journalName=Ind Crops Prod, refType=null, unstructuredReference=NIE JY,LIR,JIANGZ T,et al. Screening and evaluation of radical scavenging active compounds in the essential oil from Magnolia biondii Pamp by electronic nose coupled with chemical methodology[J]. Ind Crops Prod,2020,144:112060., articleTitle=Screening and evaluation of radical scavenging active compounds in the essential oil from Magnolia biondii Pamp by electronic nose coupled with chemical methodology, refAbstract=null), Reference(id=1322922789333762817, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922779296793256, doi=null, pmid=null, pmcid=null, year=2022, volume=42, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[17], rfOrder=16, authorNames=DING L J, WU X M, ZHANG C G, journalName=Comp Biochem Physiol Part D Genomics Proteomics, refType=null, unstructuredReference=DING L J,WU X M,ZHANG C G,et al. Toxin diversity revealed by de novo transcriptome assembly for venom gland in two species of spiders(Trichonephila clavata and Sinopoda pengi)[J]. Comp Biochem Physiol Part D Genomics Proteomics,2022,42:100984., articleTitle=Toxin diversity revealed by de novo transcriptome assembly for venom gland in two species of spiders(Trichonephila clavata and Sinopoda pengi), refAbstract=null), Reference(id=1322922789413454594, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922779296793256, doi=null, pmid=null, pmcid=null, year=2023, volume=41, issue=4, pageStart=239, pageEnd=243,后插41-后插42, url=null, language=null, rfNumber=[18], rfOrder=17, authorNames=顾小亮, 杨月娥, 杨志斌, journalName=中华中医药学刊, refType=null, unstructuredReference=顾小亮,杨月娥,杨志斌,等. 不同产地棒毛络新妇蜘蛛的脂溶性成分GC-MS分析[J]. 中华中医药学刊,2023,41(4):239-243,后插41-后插42., articleTitle=不同产地棒毛络新妇蜘蛛的脂溶性成分GC-MS分析, refAbstract=null), Reference(id=1322922789488952067, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922779296793256, doi=null, pmid=null, pmcid=null, year=2021, volume=43, issue=4, pageStart=1103, pageEnd=1107, url=null, language=null, rfNumber=[19], rfOrder=18, authorNames=赵彤, 邵瑾, 杨颖, journalName=中成药, refType=null, unstructuredReference=赵彤,邵瑾,杨颖,等. 藏紫菀不同溶剂提取物的体外抗氧化活性[J]. 中成药,2021,43(4):1103-1107., articleTitle=藏紫菀不同溶剂提取物的体外抗氧化活性, refAbstract=null), Reference(id=1322922789551866628, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922779296793256, doi=null, pmid=null, pmcid=null, year=2022, volume=40, issue=3, pageStart=139, pageEnd=142, url=null, language=null, rfNumber=[20], rfOrder=19, authorNames=王立抗, 陈鸿庚, 黄智霖, journalName=中华中医药学刊, refType=null, unstructuredReference=王立抗,陈鸿庚,黄智霖,等. 牛大力不同部位总黄酮、多酚含量及其抗氧化活性研究[J]. 中华中医药学刊,2022,40(3):139-142., articleTitle=牛大力不同部位总黄酮、多酚含量及其抗氧化活性研究, refAbstract=null), Reference(id=1322922789614781189, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922779296793256, doi=null, pmid=null, pmcid=null, year=2015, volume=144, issue=null, pageStart=830, pageEnd=835, url=null, language=null, rfNumber=[21], rfOrder=20, authorNames=SHI Q Y, CHEN JL, ZHOU Q F, journalName=Talanta, refType=null, unstructuredReference=SHI Q Y,CHEN JL,ZHOU Q F,et al. Indirect identification of antioxidants in Polygalae Radix through their reaction with 2,2-diphenyl-1-picrylhydrazyl and subsequent HPLC-ESI-QTOF-MS/MS[J]. Talanta,2015,144:830-835., articleTitle=Indirect identification of antioxidants in Polygalae Radix through their reaction with 2,2-diphenyl-1-picrylhydrazyl and subsequent HPLC-ESI-QTOF-MS/MS, refAbstract=null), Reference(id=1322922789669307142, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922779296793256, doi=null, pmid=null, pmcid=null, year=2013, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[22], rfOrder=21, authorNames=赵育卉, journalName=null, refType=null, unstructuredReference=赵育卉. 盐生海芦笋及其内生真菌Salicorn-5次级代谢产物研究[D]. 南京:南京农业大学,2013., articleTitle=盐生海芦笋及其内生真菌Salicorn-5次级代谢产物研究, refAbstract=null), Reference(id=1322922789732221703, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922779296793256, doi=null, pmid=null, pmcid=null, year=2011, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[23], rfOrder=22, authorNames=任永娜, journalName=null, refType=null, unstructuredReference=任永娜. SLPE的抗氧化、抗肿瘤成分分析及活性检测[D]. 石家庄:河北师范大学,2011., articleTitle=SLPE的抗氧化、抗肿瘤成分分析及活性检测, refAbstract=null), Reference(id=1322922789790941960, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922779296793256, doi=null, pmid=null, pmcid=null, year=2015, volume=31, issue=5, pageStart=930, pageEnd=932, url=null, language=null, rfNumber=[24], rfOrder=23, authorNames=袁子民, 刘欢, 贾天柱, journalName=长春中医药大学学报, refType=null, unstructuredReference=袁子民,刘欢,贾天柱,等. 肉豆蔻及炮制品醇提取物的抗氧化作用[J]. 长春中医药大学学报,2015,31(5):930-932., articleTitle=肉豆蔻及炮制品醇提取物的抗氧化作用, refAbstract=null), Reference(id=1322922789887410953, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922779296793256, doi=null, pmid=null, pmcid=null, year=2022, volume=51, issue=4, pageStart=1077, pageEnd=1082, url=null, language=null, rfNumber=[25], rfOrder=24, authorNames=周军伟, 陈建发, 万代红, journalName=应用化工, refType=null, unstructuredReference=周军伟,陈建发,万代红,等. 酚类抗氧剂BHT的应用和降解路径的研究进展[J]. 应用化工,2022,51(4):1077-1082., articleTitle=酚类抗氧剂BHT的应用和降解路径的研究进展, refAbstract=null), Reference(id=1322922789962908426, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922779296793256, doi=null, pmid=null, pmcid=null, year=2022, volume=30, issue=3, pageStart=23, pageEnd=32, url=null, language=null, rfNumber=[26], rfOrder=25, authorNames=SINCLAIR A J, journalName=粮油食品科技, refType=null, unstructuredReference=SINCLAIR A J. 世界食物供应中亚油酸含量高对人体健康的影响[J]. 粮油食品科技,2022,30(3):23-32., articleTitle=世界食物供应中亚油酸含量高对人体健康的影响, refAbstract=null), Reference(id=1322922791628047115, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922779296793256, doi=null, pmid=null, pmcid=null, year=2022, volume=47, issue=19, pageStart=5158, pageEnd=5170, url=null, language=null, rfNumber=[27], rfOrder=26, authorNames=徐水宇, 庄怡雪, 詹志来, journalName=中国中药杂志, refType=null, unstructuredReference=徐水宇,庄怡雪,詹志来,等. 鸦胆子的化学成分、药理作用研究进展及其质量标志物的预测分析[J]. 中国中药杂志,2022,47(19):5158-5170., articleTitle=鸦胆子的化学成分、药理作用研究进展及其质量标志物的预测分析, refAbstract=null), Reference(id=1322922791749681932, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922779296793256, doi=null, pmid=null, pmcid=null, year=2023, volume=36, issue=3, pageStart=1, pageEnd=3,8, url=null, language=null, rfNumber=[28], rfOrder=27, authorNames=金青哲, 王月, 方靖凯, journalName=粮食与油脂, refType=null, unstructuredReference=金青哲,王月,方靖凯,等. 油酸和高油酸油脂对人体健康的影响研究进展[J]. 粮食与油脂,2023,36(3):1-3,8., articleTitle=油酸和高油酸油脂对人体健康的影响研究进展, refAbstract=null), Reference(id=1322922791820985101, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922779296793256, doi=null, pmid=null, pmcid=null, year=2020, volume=12, issue=1, pageStart=35, pageEnd=null, url=null, language=null, rfNumber=[29], rfOrder=28, authorNames=ZHAO F Q, WANG P, LUCARDI R D, journalName=Toxins, refType=null, unstructuredReference=ZHAO F Q,WANG P,LUCARDI R D,et al. Natural sources and bioactivities of 2,4-di-tert-butylphenol and its analogs[J]. Toxins,2020,12(1):35., articleTitle=Natural sources and bioactivities of 2,4-di-tert-butylphenol and its analogs, refAbstract=null), Reference(id=1322922791883899662, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922779296793256, doi=null, pmid=null, pmcid=null, year=2013, volume=16, issue=11, pageStart=977, pageEnd=983, url=null, language=null, rfNumber=[30], rfOrder=29, authorNames=CHOI SJ, KIM J K, KIM H K, journalName=JMed Food, refType=null, unstructuredReference=CHOI SJ,KIM J K,KIM H K,et al. 2,4-Di-tert-butylphenol from sweet potato protects against oxidative stress in PC12 cells and in mice[J]. JMed Food,2013,16(11):977-983., articleTitle=2,4-Di-tert-butylphenol from sweet potato protects against oxidative stress in PC12 cells and in mice, refAbstract=null), Reference(id=1322922791946814223, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922779296793256, doi=null, pmid=null, pmcid=null, year=2018, volume=1865, issue=5, pageStart=675, pageEnd=683, url=null, language=null, rfNumber=[31], rfOrder=30, authorNames=SONG Y W, LIM Y, CHO S K, journalName=Biochim Biophys Acta Mol Cell Res, refType=null, unstructuredReference=SONG Y W,LIM Y,CHO S K. 2,4-Di-tert-butylphenol,a potential HDAC6 inhibitor,induces senescence and mitotic catastrophe in human gastric adenocarcinoma AGS cells[J]. Biochim Biophys Acta Mol Cell Res,2018,1865(5):675-683., articleTitle=2,4-Di-tert-butylphenol,a potential HDAC6 inhibitor,induces senescence and mitotic catastrophe in human gastric adenocarcinoma AGS cells, refAbstract=null), Reference(id=1322922792026506000, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922779296793256, doi=null, pmid=null, pmcid=null, year=2023, volume=195, issue=11, pageStart=6572, pageEnd=6585, url=null, language=null, rfNumber=[32], rfOrder=31, authorNames=KAARI M, JOSEPH J, MANIKKAM R, journalName=Appl Biochem Biotechnol, refType=null, unstructuredReference=KAARI M,JOSEPH J,MANIKKAM R,et al. A novel finding:2,4-Di-tert-butylphenol from Streptomyces bacillaris ans2 effective against Mycobacterium tuberculosis and cancer cell lines[J]. Appl Biochem Biotechnol,2023,195(11):6572-6585., articleTitle=A novel finding:2,4-Di-tert-butylphenol from Streptomyces bacillaris ans2 effective against Mycobacterium tuberculosis and cancer cell lines, refAbstract=null), Reference(id=1322922792097809169, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922779296793256, doi=null, pmid=null, pmcid=null, year=2012, volume=135, issue=4, pageStart=2366, pageEnd=2371, url=null, language=null, rfNumber=[33], rfOrder=32, authorNames=QIU J Y, CHEN L L, ZHU QJ, journalName=Food Chem, refType=null, unstructuredReference=QIU J Y,CHEN L L,ZHU QJ,et al. Screening natural antioxidants in peanut shell using DPPH-HPLC-DAD-TOF/MSmethods[J]. Food Chem,2012,135(4):2366-2371., articleTitle=Screening natural antioxidants in peanut shell using DPPH-HPLC-DAD-TOF/MSmethods, refAbstract=null), Reference(id=1322922792160723730, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922779296793256, doi=null, pmid=null, pmcid=null, year=2017, volume=12, issue=1, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[34], rfOrder=33, authorNames=QIU JY, CHENX Y, NETRUSOV A I, journalName=PLoS One, refType=null, unstructuredReference=QIU JY,CHENX Y,NETRUSOV A I,et al. Screening and identifying antioxidative components in Ginkgo biloba pollen by DPPHHPLC-PAD coupled with HPLC-ESI-MS2[J]. 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DPPH浓度/(mg·mL-1)3号峰7号峰9号峰10号峰13号峰14号峰17号峰18号峰19号峰20号峰21号峰
0.250.0610.5514.6674.04-0.280.2128.8810.6270.5668.2418.59
0.590.5517.3824.2471.0321.8117.7941.9055.6576.6870.9589.22
1100.0018.9126.557.7212.4511.0734.9558.4778.5158.2190.12
2100.0017.4634.8251.3714.2419.3243.8659.3282.4965.9492.80
), ArticleFig(id=1322922785537917669, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922779296793256, language=CN, label=表1, caption=

不同浓度DPPH对峰面积降低率的影响

, figureFileSmall=null, figureFileBig=null, tableContent=
DPPH浓度/(mg·mL-1)3号峰7号峰9号峰10号峰13号峰14号峰17号峰18号峰19号峰20号峰21号峰
0.250.0610.5514.6674.04-0.280.2128.8810.6270.5668.2418.59
0.590.5517.3824.2471.0321.8117.7941.9055.6576.6870.9589.22
1100.0018.9126.557.7212.4511.0734.9558.4778.5158.2190.12
2100.0017.4634.8251.3714.2419.3243.8659.3282.4965.9492.80
), ArticleFig(id=1322922787186279142, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922779296793256, language=EN, label=null, caption=null, figureFileSmall=null, figureFileBig=null, tableContent=
峰号保留时间/(min)化合物名称分子式相对含量/(%)
14.96正十一烷HendecaneC11H240.86
25.74正十二烷DodecaneC12H260.55
37.942,4-二叔丁基苯酚2,4-Di-tert-butylphenolC14H22O2.67
48.71二苯胺DiphenylamineC12H11N1.63
59.15肉豆蔻酸甲酯Methyl myristateC15H30O20.70
69.35肉豆蔻酸Myristic acidC14H28O20.40
710.14棕榈油酸甲酯Methyl palmitoleateC17H32O27.36
810.24十三烷酸甲酯Methyl tridecanoateC17H34O28.32
910.42棕榈酸Palmitic acidC16H32O25.85
1010.61棕榈酸乙酯Palmitic acid ethyl esterC18H36O24.43
1110.6910-二十一碳烯10-HeneicoseneC12H420.82
1210.81十七烷酸甲酯Methyl margarateC18H36O20.50
1311.27亚油酸甲酯Methyl linoleateC19H34O210.34
1411.31油酸甲酯Methyl oleateC19H36O218.46
1511.3413-十八碳烯酸甲酯13-Octadecylenoic acid methyl esterC19H36O27.37
1611.4616-甲基十七烷酸甲酯16-Methylheptadecanoic acid methyl esterC19H38O23.06
1711.56油酸Oleic acidC18H34O22.80
1811.60亚油酸Linoleic acidC18H32O23.26
1911.74亚油酸乙酯Ethyl linoleateC20H36O23.39
2011.77油酸乙酯Ethyl oleateC20H38O25.14
2111.95硬脂酸乙酯Ethyl stearateC20H40O21.23
2213.1718-甲基十九烷酸甲酯Methyl 18-methylnonade-canoateC21H42O20.53
), ArticleFig(id=1322922787278553831, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922779296793256, language=CN, label=表2, caption=

棒毛络新妇油脂的化学成分

, figureFileSmall=null, figureFileBig=null, tableContent=
峰号保留时间/(min)化合物名称分子式相对含量/(%)
14.96正十一烷HendecaneC11H240.86
25.74正十二烷DodecaneC12H260.55
37.942,4-二叔丁基苯酚2,4-Di-tert-butylphenolC14H22O2.67
48.71二苯胺DiphenylamineC12H11N1.63
59.15肉豆蔻酸甲酯Methyl myristateC15H30O20.70
69.35肉豆蔻酸Myristic acidC14H28O20.40
710.14棕榈油酸甲酯Methyl palmitoleateC17H32O27.36
810.24十三烷酸甲酯Methyl tridecanoateC17H34O28.32
910.42棕榈酸Palmitic acidC16H32O25.85
1010.61棕榈酸乙酯Palmitic acid ethyl esterC18H36O24.43
1110.6910-二十一碳烯10-HeneicoseneC12H420.82
1210.81十七烷酸甲酯Methyl margarateC18H36O20.50
1311.27亚油酸甲酯Methyl linoleateC19H34O210.34
1411.31油酸甲酯Methyl oleateC19H36O218.46
1511.3413-十八碳烯酸甲酯13-Octadecylenoic acid methyl esterC19H36O27.37
1611.4616-甲基十七烷酸甲酯16-Methylheptadecanoic acid methyl esterC19H38O23.06
1711.56油酸Oleic acidC18H34O22.80
1811.60亚油酸Linoleic acidC18H32O23.26
1911.74亚油酸乙酯Ethyl linoleateC20H36O23.39
2011.77油酸乙酯Ethyl oleateC20H38O25.14
2111.95硬脂酸乙酯Ethyl stearateC20H40O21.23
2213.1718-甲基十九烷酸甲酯Methyl 18-methylnonade-canoateC21H42O20.53
), ArticleFig(id=1322922787370828520, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922779296793256, language=EN, label=null, caption=null, figureFileSmall=null, figureFileBig=null, tableContent=
峰号保留时间/(min)化合物名称反应前峰面积反应后峰面积峰面积降低率/(%)
14.96正十一烷15 74314 3678.74
25.74正十二烷10 4799 5279.09
37.942,4-二叔丁基苯酚47 251446690.55
59.15肉豆蔻酸甲酯11 38810 3419.19
69.35肉豆蔻酸5 011450910.01
710.14棕榈油酸甲酯147 278121 67717.38
910.42棕榈酸91 16869 06924.24
1010.61棕榈酸乙酯71 21020 63371.03
1110.6910-二十一碳烯10 35593489.73
1311.27亚油酸甲酯279 425218 46921.81
1411.31油酸甲酯359 529295 56217.79
1711.56油酸46 97727 29541.90
1811.60亚油酸54 66424 24655.65
1911.74亚油酸乙酯56 79713 24276.68
2011.77油酸乙酯86 14225 02870.95
2111.95硬脂酸乙酯15 567167889.22
), ArticleFig(id=1322922787454714601, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922779296793256, language=CN, label=表3, caption=

棒毛络新妇油脂中抗氧化成分

, figureFileSmall=null, figureFileBig=null, tableContent=
峰号保留时间/(min)化合物名称反应前峰面积反应后峰面积峰面积降低率/(%)
14.96正十一烷15 74314 3678.74
25.74正十二烷10 4799 5279.09
37.942,4-二叔丁基苯酚47 251446690.55
59.15肉豆蔻酸甲酯11 38810 3419.19
69.35肉豆蔻酸5 011450910.01
710.14棕榈油酸甲酯147 278121 67717.38
910.42棕榈酸91 16869 06924.24
1010.61棕榈酸乙酯71 21020 63371.03
1110.6910-二十一碳烯10 35593489.73
1311.27亚油酸甲酯279 425218 46921.81
1411.31油酸甲酯359 529295 56217.79
1711.56油酸46 97727 29541.90
1811.60亚油酸54 66424 24655.65
1911.74亚油酸乙酯56 79713 24276.68
2011.77油酸乙酯86 14225 02870.95
2111.95硬脂酸乙酯15 567167889.22
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基于DPPH联合GC-MS技术快速筛选棒毛络新妇油脂的抗氧化成分
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王诗雨 , 吕恒 , 高萌 , 陈彤彤 , 赵晨曦 , 罗建蓉
中华中医药学刊 | 国家项目点击 2025,43(2): 108-111
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中华中医药学刊 |国家项目点击 2025 , 43 (2) : 108 -111
基于DPPH联合GC-MS技术快速筛选棒毛络新妇油脂的抗氧化成分
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王诗雨, 吕恒, 高萌, 陈彤彤, 赵晨曦, 罗建蓉
作者信息
  • 大理大学云南省昆虫生物医药研发重点实验室,大理大学药学院,云南 大理 671000
通讯作者:
罗建蓉(1979-),女(傣族),云南玉溪人,副教授,硕士研究生导师,研究方向:药效物质基础及质量标准。E-mail:。
作者简介:

王诗雨(1998-),女,黑龙江齐齐哈尔人,硕士在读,研究方向:药效物质基础。

Rapid Screening of Antioxidants of Oil Extracted from Bangmaoluoxinfu(Trichonephila clavata)Based on GC-MS Coupled with DPPH Assay
Shiyu WANG, Heng LYU, Meng GAO, Tongtong CHEN, Chenxi ZHAO, Jianrong LUO
Affiliations
  • Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R & D,College of Pharmacy,Dali University,Dali 671000,Yunnan,China
出版时间: 2025-02-10 doi: 10.13193/j.issn.1673-7717.2025.02.025
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目的

建立1,1-二苯基-2-三硝基苯肼自由基(1,1-Diphenyl-2-picrylhydrazyl,DPPH)联合气相色谱和质谱联用技术(Gas Chromatography-Mass Spectrometry,GC-MS)快速筛选棒毛络新妇油脂中的抗氧化活性成分。

方法

采用DPPH自由基化学反应与GC-MS色谱分析相结合的方法进行研究,通过分析棒毛络新妇油脂与自由基反应前后色谱图的变化来筛选抗氧化成分,并以各化合物的色谱峰面积降低率评价其抗氧化活性。同时进行方法学考察,并采用酶标仪法测定对照品的自由基清除能力,以验证DPPH-GC-MS方法的抗氧化成分筛选结果。

结果

从棒毛络新妇油脂中共鉴定出22个化合物,占总量的89.67%,主要为脂肪酸和脂肪酸酯,其中油酸甲酯和亚油酸甲酯相对含量较高。通过DPPH-GC-MS方法从中快速筛选到16个抗氧化成分,其中2,4-二叔丁基苯酚、硬脂酸乙酯、亚油酸、亚油酸乙酯、油酸乙酯和棕榈酸乙酯的抗氧化能力较强。活性验证结果表明DPPH-GC-MS方法可靠,可对天然产物中的抗氧化活性物质进行快速筛选和鉴定。

结论

DPPH-GC-MS方法可简便高效地筛选出棒毛络新妇油脂中的抗氧化成分,为进一步开发利用棒毛络新妇资源提供实验依据。

棒毛络新妇  /  油脂  /  DPPH-GC-MS  /  抗氧化成分
Objective

To establish a gas chromatography mass spectrometry(GC-MS)technique using 1,1-Diphenyl-2-picrylhydrazyl(DPPH)as a free radical for rapid screening of antioxidant active components in the oil extracted from Bangmaoluoxinfu(Trichonephila clavata).

Methods

The method combining GC-MSchromatographic analysis with DPPH radical chemical reaction was used to screen antioxidant components by analyzing the changes in the chromatographic of the oil extracted from Bangmaoluoxinfu(Trichonephila clavata)before and after reaction with DPPH. Furthermore,the antioxidant activity of each compound was evaluated by the rate of reduction of the peak area of the chromatograms. At the same time,to verify the screening results of antioxidants by the DPPH-GC-MSmethod,a methodological study was carried out,and the free radical scavenging ability of the control product was determined by a microplate reader.

Result

A total of 22 compounds were identified from the oil extracted from Bangmaoluoxinfu(Trichonephila clavata),accounting for 89.67% of the total,mainly consisting of fatty acids and esters,with relatively high levels of methyl oleate and methyl linoleate. Sixteen antioxidants were rapidly screened from them by the DPPH-GC-MSmethod,among which 2,4-Di-tert-butylphenol,ethyl stearate,linoleic acid,ethyl linoleate,ethyl oleate and palmitic acid ethyl ester showed strong antioxidant capacity. The results of activity verification showed that the DPPH-GC-MS method was reliable and can be used for rapid screening and identification of antioxidants in natural products.

Conclusion

The DPPH-GC-MSmethod can simply and efficiently screen out the antioxidants in the oil extracted from Bangmaoluoxinfu(Trichonephila clavata),and provided the experimental basis for further development and utilization of Bangmaoluoxinfu(Trichonephila clavata)resources.

Bangmaoluoxinfu(Trichonephila clavata)  /  oil  /  DPPH-GC-MS  /  antioxidants
王诗雨, 吕恒, 高萌, 陈彤彤, 赵晨曦, 罗建蓉. 基于DPPH联合GC-MS技术快速筛选棒毛络新妇油脂的抗氧化成分. 中华中医药学刊, 2025 , 43 (2) : 108 -111 . DOI: 10.13193/j.issn.1673-7717.2025.02.025
Shiyu WANG, Heng LYU, Meng GAO, Tongtong CHEN, Chenxi ZHAO, Jianrong LUO. Rapid Screening of Antioxidants of Oil Extracted from Bangmaoluoxinfu(Trichonephila clavata)Based on GC-MS Coupled with DPPH Assay[J]. Chinese Archives of Traditional Chinese Medicine, 2025 , 43 (2) : 108 -111 . DOI: 10.13193/j.issn.1673-7717.2025.02.025
棒毛络新妇(Trichonephila clavata)为节肢动物门蛛形纲蜘蛛目圆蛛科毛络新妇属蜘蛛[1],民间多称其为花蜘蛛、金丝蜘蛛[2],广泛分布于云贵高原,为一年生节肢动物[3]。蜘蛛具有悠久的药用历史,早在唐代《本草拾遗》中便记载蜘蛛“主蛇毒,温疟,霍乱,止呕逆”。《本草纲目》记载蜘蛛“微寒、有小毒,主治婴儿口噤、泄痛脱肛、结核、蛇虫叮咬等”。明代陈文治编撰的《疡科选粹》记载棒毛络新妇的临床验方“花蛛散”,用于治疗内外痔。《中国药用动物志》中记载棒毛络新妇以全体入药,微苦、凉,有小毒,具有解热消肿、截疟杀虫、退黄生肌的功效,主治温证、瘰疬、疮肿、毒蛇咬伤等[4]。现代研究发现棒毛络新妇提取物对人口腔上皮癌KB细胞株、人肺癌A549细胞和白血病HL-60细胞具有显著抑制作用,具有抑制金黄色葡萄球菌、链球菌和大肠杆菌生长的作用,具有抑制单纯疱疹病毒HSV-1生长的作用[5-8]。棒毛络新妇毒液中含有的N-酰基氨基酸JSTX-3具有抗癫痫作用[9-10]。蛛丝中的主要成分黄尿酸可抑制广谱细菌生长[11]。
1,1-二苯基-2-三硝基苯肼(1,1-Diphenyl-2-picrylhydrazyl,DPPH)是一种稳定存在的自由基,当抗氧剂与DPPH自由基反应时抗氧剂把电子和质子传递给DPPH自由基,抗氧剂被氧化结构改变[12-13]。基于此原理,可用高效液相色谱法(High Performance Liquid Chromatography,HPLC)或气相色谱(Gas Chromatography,GC)分析天然产物与DPPH自由基反应前后色谱峰面积的变化,从复杂成分中快速筛选出抗氧化活性成分[14]。对于挥发性天然产物更适于用DPPH自由基结合GC进行检测,WANG X等[15]运用DPPH-GC-质谱(Mass Spectrometry,MS)方法在温郁金挥发油中筛选到5种抗氧化活性成分。NIE JY等[16]运用DPPH、ABTS和羟基等自由基联合GC电子鼻法筛选八角茴香精油中的抗氧化成分。目前棒毛络新妇的研究主要集中于蛛毒生物活性的研究[9,17],对于棒毛络新妇全虫化学成分的研究较少。课题组前期采用GCMS分析不同产地的棒毛络新妇油脂,发现其主要成分类型为脂肪酸[18]。鉴于此,本研究通过测定棒毛络新妇油脂的DPPH自由基清除率来评估其抗氧化能力,并进一步建立DPPHGC-MS方法,通过比较与DPPH自由基反应前后色谱图的变化,从而快速筛选和鉴定棒毛络新妇油脂中的抗氧化活性物质。以期综合评价棒毛络新妇油脂的抗氧化活性,并明确其抗氧化物质基础,丰富棒毛络新妇药效物质基础数据,为棒毛络新妇资源的开发利用提供科学依据。
7890A/5975C型气相色谱-质谱联用分析仪(美国Agilent公司);Varioskan LUX多功能酶标仪(美国Thermo Fisher Scientific公司);SB25-12D型超声波清洗机(宁波新芝生物科技股份有限公司);R-210型旋转蒸发仪(瑞士BUCHI公司);FA2104型电子天平(上海舜宇恒平科学仪器有限公司)。
棒毛络新妇样品于2022年10月份采集于云南大理,经大理大学杨自忠教授鉴定为毛络新妇属蜘蛛棒毛络新妇(Trichonephila clavata)。DPPH自由基(日本东京化成工业株式会社,批号:KN54G-AN);维生素C(日本东京化成工业株式会社,批号:8ABZI-JE);BHT(日本东京化成工业株式会社,批号:KSSZD-DB);油酸(上海源叶生物科技有限公司,批号:M21GB149066);肉豆蔻酸(上海源叶生物科技有限公司,批号:J051IS219065);甲醇(分析纯,西陇科学股份有限公司,批号:190222)。
棒毛络新妇鲜品去足后,称取95.8 g研碎,用80%乙醇超声提取6次,每次30 min,提取液减压浓缩至小体积,用石油醚萃取3次,萃取液经回收溶剂后冻干,制得棒毛络新妇油脂,得率为6.5%。
采用倍比稀释法配制浓度为4、2、1、0.5、0.25 mg·mL-1的棒毛络新妇油脂甲醇溶液。参考文献[19-20]方法,分别取棒毛络新妇油脂溶液50μL和0.2 mmol·L-1的DPPH甲醇溶液50μL于96孔板中混匀,室温下避光反应30 min,517 nm处测吸光度(A),Vc为阳性对照,设3个重复,取平均值,按公式计算清除率。DPPH自由基清除率=[1-(A1-A2)/A0]×100%,式中A1为供试品+DPPH,A2为供试品+甲醇,A0为DPPH+甲醇。
检测结果由图1可知,棒毛络新妇油脂具有一定抗氧化能力,其DPPH自由基清除能力与浓度呈明显的正相关。当油脂浓度为4.0 mg·mL-1时,DPPH清除率达91.22%,其清除DPPH的IC50值为1.45 mg·mL-1。
GC条件:Agilent HP-5MS石英弹性毛细管柱(30 m×250μm,0.25μm),载气为高纯氦,进样量为1.0μL,进样口温度230℃,分流比10:1,柱流量1.0 mL·min-1;升温程序:起始柱温60℃,保持1 min,以20℃·min-1升温至240℃,后以1℃·min-1升温至250℃,再按5℃·min-1升温至260℃。
MS条件:EI离子源,离子源温度230℃,电离能量为70 eV;四极杆温度150℃;质量扫描范围为全扫描;溶剂延迟3 min。质谱采用NIST11. L标准谱库进行检索。
DPPH自由基浓度是影响筛选抗氧化成分的关键因素,浓度过低会造成低活性或低含量的抗氧化成分无法有效筛选;浓度过高会使其分解产物增多,增加色谱峰分离难度,干扰抗氧化成分筛选[14],故而本文对DPPH自由基浓度进行考察[15,21]。配制2、1、0.5、0.25 mg·mL-1的DPPH甲醇溶液,分别取各浓度的DPPH溶液0.5 mL与1 mg·mL-1的棒毛络新妇油脂溶液0.5 mL混匀,于37℃下避光反应30 min,将反应液按“2.3.1”项下条件进行检测,考察不同浓度DPPH自由基对色谱峰峰面积降低率的影响。
结果(插页ⅩⅣ图1、表1)显示,不同浓度的DPPH自由基对样品中的抗氧化活性色谱峰峰面积的降低程度有明显的影响;随DPPH浓度增大,峰面积降低的色谱峰数目增多,同时主要活性色谱峰的峰面积降低率也逐渐增大。当DPPH自由基浓度为0.5 mg·mL-1时,峰面积降低的变化最明显;而浓度继续增大至≥1 mg·mL-1时,峰面积降低率无更明显增加,故后续实验以0.5 mg·mL-1为最佳的DPPH自由基浓度。
取1 mg·mL-1的棒毛络新妇油脂甲醇溶液与0.5 mg·mL-1的DPPH自由基甲醇溶液等体积混匀,37℃下避光反应30 min,制得反应后样品;加等体积甲醇代替自由基试剂,制得反应前样品,将各样品按“2.3.1”项下条件进行检测,通过计算分析反应前后各色谱峰的峰面积降低率,来筛选抗氧化活性成分。
棒毛络新妇油脂经GC-MS分析,从中共鉴定22个化学成分(见表2和插页ⅩⅣ图2),相对含量占总量89.67%,主要成分类型为脂肪酸和脂肪酸酯,另外检出少量烷烃和酚类。检出脂肪酸酯的数目最多为13个,其中油酸甲酯和亚油酸甲酯的含量最高,其相对含量均大于10%。
与DPPH自由基反应后,棒毛络新妇油脂的色谱图中共16个色谱峰的峰面积有不同程度减少。其中3、10、18、19、20和21号峰的峰面积减少明显,峰面积降低率超过50%,提示其对应的化合物2,4-二叔丁基苯酚、棕榈酸乙酯、亚油酸、亚油酸乙酯、油酸乙酯和硬脂酸乙酯具有较强的DPPH自由基清除能力,为棒毛络新妇油脂中的强抗氧化成分,其中以油酸乙酯、亚油酸乙酯和亚油酸为丰量抗氧化成分。另外,1、2、5、6、7、9、11、13、14和17号峰的峰面积呈现一定程度减少,峰面积降低率为8.74%~41.90%,提示正十一烷、正十二烷、肉豆蔻酸甲酯、肉豆蔻酸、棕榈油酸甲酯、棕榈酸、10-二十一碳烯、亚油酸甲酯、油酸甲酯和油酸等成分在棒毛络新妇油脂中发挥一定程度的抗氧化作用。见表3。
将2,6-二叔丁基对甲酚(BHT)、肉豆蔻酸和油酸对照品配制成0.2、0.1、0.05、0.025和0.012 5 mg·mL-1系列浓度的混合对照品溶液,按“2.3.1”项下色谱条件检测。以浓度为横坐标,对照品色谱峰面积为纵坐标,进行线性回归,得到3个对照品的线性回归方程(见表4)。结果表明,在上述色谱条件下,3个对照品在0.012 5~0.2 mg·mL-1范围内呈良好的线性关系。
取混合对照品溶液,按“2.3.1”项下条件连续进样6次,记录峰面积,计算RSD值。结果得BHT、肉豆蔻酸和油酸对照品峰面积的RSD值分别为1.64%、0.99%和1.47%,表明仪器精密度良好。
取同一批棒毛络新妇样品,按“2.1”方法平行制备6份供试品溶液,按“2.3.1”项下条件进行分析,记录保留时间及峰面积,计算RSD值。所鉴定出的22种成分保留时间的RSD值在0~0.01%之间,峰面积的RSD值在0.19%~5.55%之间,表明在该色谱条件下方法重复性良好。
取棒毛络新妇油脂甲醇溶液分别于0、2、4、8、12、24 h按“2.3.1”项下条件进行检测,计算保留时间及峰面积的RSD值。所鉴定出的22种成分保留时间RSD值在0~0.14%之间,峰面积RSD值在2.58%~7.86%之间,表明供试品溶液24 h内稳定。
油酸和肉豆蔻酸为棒毛络新妇油脂中的化学成分,油酸具有DPPH自由基清除能力[22-23];肉豆蔻酸表现出微弱的DPPH自由基清除能力[24];BHT为人工合成抗氧剂,具有很强的抗氧化活性,常用作阳性对照[25],故而选择BHT、油酸和肉豆蔻酸为对照品进行验证。取“2.4.1”项下1 mg·mL-1的混合对照品溶液,采用DPPHGC-MS方法按“2.3.3”项下操作,来研究混合对照品中的抗氧化活性成分;并采用酶标仪法按“2.2”项检测对照品的自由基清除率,加以验证其抗氧化活性。
DPPH-GC-MS法检测结果(插页ⅩⅣ图3)显示混合对照品中BHT的峰面积降低率最高为93.18%,其次为油酸其峰面积降低率为35.32%,而肉豆蔻酸仅降低6.31%,3种对照品的抗氧化能力强弱为BHT>油酸>肉豆蔻酸。酶标仪法检测结果(插页ⅩⅣ图4)显示,3个对照品中BHT的抗氧化能力最强,油酸次之,肉豆蔻酸最弱。由此可见,DPPH-GC-MS方法的筛选结果与酶标仪法的检测结果一致,表明DPPH-GC-MS方法的研究结果可靠。
棒毛络新妇是一种广泛分布于云贵高原的药用蜘蛛,目前未见有关棒毛络新妇抗氧化活性及其成分的相关报道。本研究利用DPPH自由基与棒毛络新妇油脂反应,通过GC-MS分析反应前后色谱峰的变化,快速发现棒毛络新妇油脂中所含有的16种抗氧化活性成分。含量较高的抗氧化活性成分为不饱和脂肪酸及其酯类成分,主要有亚油酸、油酸、亚油酸甲酯、油酸甲酯、亚油酸乙酯和油酸乙酯。其中,亚油酸是一种人体正常代谢不可缺少的但自身又无法合成的必需脂肪酸[26],可抑制动脉血栓形成、降低人体血清中胆固醇水平,具有防治高血压、预防癌症、调节人体免疫的功能[27];油酸是人体最易消化吸收的脂肪酸,具有保护心脑血管健康、调节血脂水平的作用[28]。抗氧化活性最强的成分2,4-二叔丁基苯酚对过氧化氢诱导的PC12细胞和小鼠的氧化应激起保护作用[29-30],对人胃腺癌AGS细胞具有抑制作用[31],并具有良好的抗结核活性[32]。
在抗氧化成分筛选实验中,观察到与DPPH自由基反应后,色谱图中新增加3个色谱峰(Ⅰ、Ⅱ和Ⅲ号),其峰面积随DPPH自由基浓度增大而增加,结合质谱信息判断为DPPH自由基及其分解产物的色谱峰。在QIU J Y等[33-34]运用DPPH-HPLC-DAD-TOF/MS方法筛选花生壳中的天然抗氧化剂及运用DPPH-HPLC-PAD筛选银杏花粉中的抗氧化成分时也出现过类似情况。因此本实验中需要考察DPPH自由基浓度,以避免浓度过高导致分解产物峰面积过大,增加色谱峰分离难度,干扰抗氧化成分筛选。
从混合物中快速筛选抗氧化活性成分是具有挑战的,传统的自由基清除率测定方法主要用于评价总提物的抗氧化活性,而针对其中单体成分的抗氧化研究需要先分离得到单体化合物再进行活性测定。DPPH-GC-MS方法采用自由基化学反应与色谱法分析相结合,可快速从混合物中筛选出抗氧化活性成分,无须进行单体成分的分离,相较于传统模式具有操作简便、耗时短等优势。本研究建立DPPH-GC-MS方法简便高效地对棒毛络新妇油脂中抗氧化活性成分进行筛选,为药用蜘蛛棒毛络新妇的进一步开发利用提供实验基础。
  • 国家自然科学基金项目(32160113)
  • 云南省地方本科高校基础研究联合专项(202101AO070274)
  • 云南省昆虫生物医药研发重点实验室开放项目(AP2022004)
  • 云南省大学生创新创业训练计划项目(202210679010)
  • 云南省“兴滇英才支持计划”团队专项(202305AS350001)
参考文献 引证文献
排序方式:
[1]
KUNTNER M,HAMILTON C A,CHENG R C,et al. Golden orbweavers ignore biological rules:phylogenomic and comparative analyses unravel a complex evolution of sexual size dimorphism[J]. Syst Biol,2019,68(4):555-572.
[2]
YONG H S,SONGSL,CHUA K O,et al. Complete mitochondrial genomes and phylogenetic relationships of the Genera Nephila and Trichonephila(Araneae,Araneoidea)[J]. Sci Rep,2021,11(1):10680.
[3]
万晓瑜,廖绍波. 云南民间食用蜘蛛初报[J]. 楚雄师范学院学报,2013,28(3):56-59.
[4]
李军德,黄璐琦,曲晓波. 中国药用动物志[M]. 2版. 福州:福建科学技术出版社,2013:432-433.
[5]
高鹏飞,刘正文,郑园园,等. 络新妇属蜘蛛提取物抗肿瘤部位的制备方法及医药用途:CN103638057A[P]. 2014-03-19.
[6]
巫秀美,郭华,冯萍,等. 抑制真菌生长的络新妇属蜘蛛有效部位的制备及其用途:CN103655631A[P]. 2014-03-26.
[7]
陈俊雅,李启艳,方春生,等. 一种络新妇属蜘蛛有效部位的制备及抗革兰氏菌医药用途:CN103638058A[P]. 2014-03-19.
[8]
杨自忠,朱振东,马艳滟,等. 一种具抗病毒功效的络新妇属蜘蛛有效部位及其制备方法:CN104042632B[P]. 2018-05-01.
[9]
SALAMONI SD,COSTA DA COSTA J,PALMA M S,et al. Antiepileptic effect of acylpolyaminetoxin JSTX-3 on rat hippocampal CA1 neurons in vitro[J]. Brain Res,2005,1048(1-2):170-176.
[10]
JIN L,FANG M Q,CHEN M R,et al. An insecticidal toxin from Nephila clavata spider venom[J]. Amino Acids,2017,49(7):1237-1245.
[11]
FUJIWARA M,KONO N,HIRAYAMA A,et al. Xanthurenic acid is the main pigment of Trichonephila clavata gold dragline silk[J]. Biomolecules,2021,11(4):563.
[12]
施树云,郭柯柯,彭胜,等. DPPH-HPLC-QTOF-MS/MS快速筛选和鉴定杜仲黑茶中抗氧化活性成分[J]. 天然产物研究与开发,2018,30(11):1913-1917.
[13]
袁永雷,王飞飞,曲丽萍. HPLC-DPPH快速发现重楼果壳总黄酮中抗氧化成分及其活性初步验证[J]. 中国药师,2021,24(8):550-552,557.
[14]
钱金珠,梁明,余意,等. HPLC-DPPH在线联用技术评价制首乌的抗氧化活性[J]. 中国药科大学学报,2022,53(2):185-191.
[15]
WANG X,ZUO G L,WANG C Y,et al. An off-line DPPHGC-MScoupling countercurrent chromatography method for screening,identification,and separation of antioxidant compounds in essential oil[J]. Antioxidants,2020,9(8):702.
[16]
NIE JY,LIR,JIANGZ T,et al. Screening and evaluation of radical scavenging active compounds in the essential oil from Magnolia biondii Pamp by electronic nose coupled with chemical methodology[J]. Ind Crops Prod,2020,144:112060.
[17]
DING L J,WU X M,ZHANG C G,et al. Toxin diversity revealed by de novo transcriptome assembly for venom gland in two species of spiders(Trichonephila clavata and Sinopoda pengi)[J]. Comp Biochem Physiol Part D Genomics Proteomics,2022,42:100984.
[18]
顾小亮,杨月娥,杨志斌,等. 不同产地棒毛络新妇蜘蛛的脂溶性成分GC-MS分析[J]. 中华中医药学刊,2023,41(4):239-243,后插41-后插42.
[19]
赵彤,邵瑾,杨颖,等. 藏紫菀不同溶剂提取物的体外抗氧化活性[J]. 中成药,2021,43(4):1103-1107.
[20]
王立抗,陈鸿庚,黄智霖,等. 牛大力不同部位总黄酮、多酚含量及其抗氧化活性研究[J]. 中华中医药学刊,2022,40(3):139-142.
[21]
SHI Q Y,CHEN JL,ZHOU Q F,et al. Indirect identification of antioxidants in Polygalae Radix through their reaction with 2,2-diphenyl-1-picrylhydrazyl and subsequent HPLC-ESI-QTOF-MS/MS[J]. Talanta,2015,144:830-835.
[22]
赵育卉. 盐生海芦笋及其内生真菌Salicorn-5次级代谢产物研究[D]. 南京:南京农业大学,2013.
[23]
任永娜. SLPE的抗氧化、抗肿瘤成分分析及活性检测[D]. 石家庄:河北师范大学,2011.
[24]
袁子民,刘欢,贾天柱,等. 肉豆蔻及炮制品醇提取物的抗氧化作用[J]. 长春中医药大学学报,2015,31(5):930-932.
[25]
周军伟,陈建发,万代红,等. 酚类抗氧剂BHT的应用和降解路径的研究进展[J]. 应用化工,2022,51(4):1077-1082.
[26]
SINCLAIR A J. 世界食物供应中亚油酸含量高对人体健康的影响[J]. 粮油食品科技,2022,30(3):23-32.
[27]
徐水宇,庄怡雪,詹志来,等. 鸦胆子的化学成分、药理作用研究进展及其质量标志物的预测分析[J]. 中国中药杂志,2022,47(19):5158-5170.
[28]
金青哲,王月,方靖凯,等. 油酸和高油酸油脂对人体健康的影响研究进展[J]. 粮食与油脂,2023,36(3):1-3,8.
[29]
ZHAO F Q,WANG P,LUCARDI R D,et al. Natural sources and bioactivities of 2,4-di-tert-butylphenol and its analogs[J]. Toxins,2020,12(1):35.
[30]
CHOI SJ,KIM J K,KIM H K,et al. 2,4-Di-tert-butylphenol from sweet potato protects against oxidative stress in PC12 cells and in mice[J]. JMed Food,2013,16(11):977-983.
[31]
SONG Y W,LIM Y,CHO S K. 2,4-Di-tert-butylphenol,a potential HDAC6 inhibitor,induces senescence and mitotic catastrophe in human gastric adenocarcinoma AGS cells[J]. Biochim Biophys Acta Mol Cell Res,2018,1865(5):675-683.
[32]
KAARI M,JOSEPH J,MANIKKAM R,et al. A novel finding:2,4-Di-tert-butylphenol from Streptomyces bacillaris ans2 effective against Mycobacterium tuberculosis and cancer cell lines[J]. Appl Biochem Biotechnol,2023,195(11):6572-6585.
[33]
QIU J Y,CHEN L L,ZHU QJ,et al. Screening natural antioxidants in peanut shell using DPPH-HPLC-DAD-TOF/MSmethods[J]. Food Chem,2012,135(4):2366-2371.
[34]
QIU JY,CHENX Y,NETRUSOV A I,et al. Screening and identifying antioxidative components in Ginkgo biloba pollen by DPPHHPLC-PAD coupled with HPLC-ESI-MS2[J]. PLoS One,2017,12(1):e0170141.
2025年第43卷第2期
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doi: 10.13193/j.issn.1673-7717.2025.02.025
  • 首发时间:2026-10-30
  • 出版时间:2025-02-10
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国家自然科学基金项目(32160113)
云南省地方本科高校基础研究联合专项(202101AO070274)
云南省昆虫生物医药研发重点实验室开放项目(AP2022004)
云南省大学生创新创业训练计划项目(202210679010)
云南省“兴滇英才支持计划”团队专项(202305AS350001)
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    大理大学云南省昆虫生物医药研发重点实验室,大理大学药学院,云南 大理 671000

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罗建蓉(1979-),女(傣族),云南玉溪人,副教授,硕士研究生导师,研究方向:药效物质基础及质量标准。E-mail:。
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2种不同金属材料的力学参数
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genus
种数
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species
占总种数比例
Percentage of
total species (%)
属
Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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