中华中医药学刊

γ-环糊精金属有机骨架负载异甘草素复合物的制备及其抗炎活性研究

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樊翠萍 ¹ , 尚鑫 ² , 刘旭洋 ¹ , 刘萌芽 ¹ , 鲁妍妍 ¹ , 蔡邦荣 ¹ , 刘改枝 ¹

中华中医药学刊 | 国家项目点击 2026,44(7): 204-209

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中华中医药学刊 |国家项目点击 2026 , 44 (7) : 204 -209

γ-环糊精金属有机骨架负载异甘草素复合物的制备及其抗炎活性研究

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樊翠萍¹, 尚鑫², 刘旭洋¹, 刘萌芽¹, 鲁妍妍¹, 蔡邦荣¹, 刘改枝¹

作者信息

^1.河南中医药大学药学院，河南　郑州　450046

^2.长春健康职业学院，吉林　长春　130012

通讯作者:

刘改枝（1981-），女，河南新乡人，教授，硕士研究生导师，博士，研究方向：中药活性成分与新药研发、药物新制剂与新技术。E-mail：liugaizhi@126.com。

作者简介:

樊翠萍（2002-），女，河南南阳人，硕士在读，研究方向：中药活性成分及天然产物修饰、新剂型研发。

Preparation and Biological Evaluation of Isoliquiritigenin-Loadedγ-Cyclodextrin Metal-Organic Framework Complex

Cuiping FAN¹, Xin SHANG², Xuyang LIU¹, Mengya LIU¹, Yanyan LU¹, Bangrong CAI¹, Gaizhi LIU¹

Affiliations

^1.School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, Henan, China

^2.Changchun Vocational College of Health, Changchun 130012, Jilin, China

出版时间: 2026-07-10 doi: 10.13193/j.issn.1673-7717.2026.07.035

文章导航

摘要

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目的

γ-环糊精金属有机骨架（γ-CD-MOF）为载体负载异甘草素（ISO）形成γ-环糊精金属有机骨架复合物（γ-CD-MOF/ISO）以改善ISO的物理化学性质及其抗炎活性。

方法

①运用水热合成法制备γ-CD-MOF，通过加热搅拌法负载ISO形成γ-CD-MOF/ISO。利用扫描电镜和红外光谱分析对其形态结构进行表征，并对其载药量进行评价。②以脂多糖（LPS）诱导的小鼠单核巨噬细胞（RAW 264.7）为炎症模型，考察γ-CD-MOF/ISO的体外抗炎活性。采用CCK-8法检测γ-CD-MOF/ISO的细胞毒性；Griess法检测细胞上清液一氧化氮（NO）水平；酶联免疫吸附测定（ELISA）法检测细胞上清液白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）水平；蛋白免疫印迹技术（WB）检测环氧化酶（COX-2）、诱导型一氧化氮合酶（iNOS）蛋白表达量。

结果

γ-CD-MOF/ISO为立方晶体，形状规则，负载ISO前后其形态和粒径无明显变化。γ-CD-MOF/ISO的载药量为5.01%±0.21%。2.5 μg/mL的γ-CD-MOF/ISO对RAW 264.7细胞活力无影响。γ-CD-MOF/ISO（0.1、0.5、2.5 μg/mL）呈浓度依赖性抑制RAW 264.7细胞NO的生成、促炎因子IL-6、TNF-α的分泌，降低COX-2和iNOS蛋白表达水平。

结论

γ-CD-MOF/ISO生物安全性良好，具有较好的抗炎活性，为治疗急性肺损伤提供新剂型。

关键词

异甘草素 / 环糊精金属有机骨架 / 急性肺损伤 / 抗炎活性

Abstract

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Objective

In order to improve the physicochemical properties of isoliquiritigenin (ISO) and its anti-inflammatory activity, ISO-loaded γ-cyclodextrin metal-organic framework complex (γ-CD-MOF/ISO) was prepared.

Methods

①γ-CD-MOF was synthesized by hydrothermal synthesis method, and ISO was loaded to form γ-CD-MOF/ISO by agitating heater.γ-CD-MOF/ISO was characterized by scanning electron microscopy (SEM) and Fourier transform infrared spectrometer (FR-IR). The drug loading of γ-CD-MOF/ISO was evaluated.②The anti-inflammatory activity of γ-CD-MOF/ISO was investigated in LPS-induced RAW 264.7 cell model in vitro. The effect of γ-CD-MOF/ISO on cell viability was detected by CCK-8 assay. NO content in the supernatant was detected by Griess assay and the levels of pro-inflammatory cytokines IL-6 and TNF-α were measured by ELISA kit. The expressions of COX-2 and iNOS in protein level were detected by Western blot (WB).

Results

The morphology of γ-CD-MOF/ISO was cubic crystals with uniform size. Drug loading efficiency ofγ-CD-MOF/ISO was 5.01%±0.21%.γ-CD-MOF/ISO at 2.5 μg/mL had no toxin in RAW 264.7 cells.γ-CD-MOF/ISO (0.1, 0.5, 2.5 μg/mL) concentration-dependently inhibited the NO production, the secretion of pro-inflammatory cytokines IL-6 and TNF-α, and COX-2 and iNOS in LPS-induced RAW 264.7 cells.

Conclusion

γ-CD-MOF/ISO exhibits high biocompability and improves the anti-inflammatory activity, which provides a new formulation in the treatment of acute lung injury.

Key words

isoliquiritigenin / cyclodextrin metal-organic framework / acute lung injury / anti-inflammatory activity

引用本文

樊翠萍, 尚鑫, 刘旭洋, 刘萌芽, 鲁妍妍, 蔡邦荣, 刘改枝. γ-环糊精金属有机骨架负载异甘草素复合物的制备及其抗炎活性研究. 中华中医药学刊, 2026 , 44 (7) : 204 -209 . DOI: 10.13193/j.issn.1673-7717.2026.07.035

Cuiping FAN, Xin SHANG, Xuyang LIU, Mengya LIU, Yanyan LU, Bangrong CAI, Gaizhi LIU. Preparation and Biological Evaluation of Isoliquiritigenin-Loadedγ-Cyclodextrin Metal-Organic Framework Complex[J]. Chinese Archives of Traditional Chinese Medicine, 2026 , 44 (7) : 204 -209 . DOI: 10.13193/j.issn.1673-7717.2026.07.035

基金

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国家自然科学基金青年科学基金项目(82104561)
中国博士后科学基金面上项目(2020M682311)
河南省高等学校重点科研项目(25A350007)

参考文献

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2026年第44卷第7期

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文章信息

doi: 10.13193/j.issn.1673-7717.2026.07.035

首发时间：2026-10-20
出版时间：2026-07-10

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基金

国家自然科学基金青年科学基金项目(82104561)

中国博士后科学基金面上项目(2020M682311)

河南省高等学校重点科研项目(25A350007)

作者信息

^1.河南中医药大学药学院，河南　郑州　450046

^2.长春健康职业学院，吉林　长春　130012

通讯作者:

刘改枝（1981-），女，河南新乡人，教授，硕士研究生导师，博士，研究方向：中药活性成分与新药研发、药物新制剂与新技术。E-mail：liugaizhi@126.com。

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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