中华中医药学刊

基于Notch信号通路探讨金龙定喘合剂调节免疫失衡治疗哮喘的作用机制

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赵姣 ¹ , 尹江涛 ¹ , 董思麟 ¹ , 张月 ² , 崔英海 ¹ , 关玉娟 ¹ , 丁晓欢 ¹

中华中医药学刊 | 国家项目点击 2026,44(8): 36-40

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中华中医药学刊 |国家项目点击 2026 , 44 (8) : 36 -40

基于Notch信号通路探讨金龙定喘合剂调节免疫失衡治疗哮喘的作用机制

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赵姣¹, 尹江涛¹, 董思麟¹, 张月², 崔英海¹, 关玉娟¹, 丁晓欢¹

作者信息

^1.辽宁中医药大学附属第二医院，辽宁　沈阳　110034

^2.辽宁中医药大学研究生院，辽宁　沈阳　110847

通讯作者:

关玉娟（1978-），女（满族），辽宁丹东人，主任中医师，博士，研究方向：中医药治疗肺系疾病。E-mail：snowthaw@163.com。

丁晓欢（1982-），女，辽宁沈阳人，副主任中医师，博士，研究方向：中医肺病、中西医结合基础及临床。E-mail：13840185127@126.com。

作者简介:

赵姣（1987-），女，辽宁葫芦岛人，副主任中医师，硕士，研究方向：中西医结合临床。

Exploring Mechanism of Jinlong Dingchuan Mixture (金龙定喘合剂) Regulating Immune Imbalance in Treating Asthma Based on Notch Signaling Pathway

Jiao ZHAO¹, Jiangtao YIN¹, Siling DONG¹, Yue ZHANG², Yinghai CUI¹, Yujuan GUAN¹, Xiaohuan DING¹

Affiliations

^1.The Second Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang 110034, Liaoning, China

^2.Graduate School of Liaoning University of Traditional Chinese Medicine, Shenyang 110847, Liaoning, China

出版时间: 2026-08-10 doi: 10.13193/j.issn.1673-7717.2026.08.008

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摘要

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目的

基于Notch信号通路探讨金龙定喘合剂调节支气管哮喘大鼠辅助性T细胞17（Th17）/调节性T细胞（Treg）平衡的作用机制。

方法

50只SD大鼠随机分为对照组、模型组、金龙定喘合剂组、Notch激动剂组及金龙定喘合剂+Notch激动剂组，每组10只。注射和吸入卵白蛋白（OVA）方式制备大鼠哮喘模型，各药物组大鼠灌胃/腹腔注射相应药物，1次/d，连续14 d。检测大鼠静息每分钟通气量（VE）、呼气峰流速（PEF），以评价肺功能；HE和Masson染色观察肺组织病理损伤与纤维化情况；检测支气管肺泡灌洗液（BALF）中总细胞计数、嗜酸性粒细胞、中性粒细胞，淋巴细胞数量；流式细胞仪测定外周血中Th17、Treg比例；ELISA测定BALF中白细胞介素（IL）-10、IL-17、转化生长因子-β（TGF-β）水平，Western blot检测肺组织中Notch1及发状分裂相关增强子1（Hes1）蛋白的表达。

结果

金龙定喘合剂组大鼠肺功能显著改善，肺组织病理损伤与纤维化明显减轻；BALF中炎症细胞数量显著减少；Th17/Treg比值显著降低，IL-10、TGF-β水平显著升高，IL-17水平显著降低；Notch1与Hes1蛋白表达水平显著降低；而Notch激动剂Jagged1可部分逆转金龙定喘合剂对哮喘大鼠的上述治疗作用。

结论

金龙定喘合剂能够改善支气管哮喘大鼠Th17/Treg平衡，抑制炎症反应，改善肺功能，其作用机制可能与抑制Notch信号通路有关。

关键词

金龙定喘合剂 / 支气管哮喘 / Notch信号通路 / Th17/Treg平衡 / 炎症反应

Abstract

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Objective

To explore the mechanism of Jinlong Dingchuan Mixture (金龙定喘合剂) in regulating the balance of helper T cell 17 (Th17)/regulatory T cell (Treg) in the bronchial asthma mice based on Notch signaling pathway.

Methods

Fifty C57BL/6 mice were randomly divided into control group (control), model group (model), Jinlong Dingchuan Mixture group (JLDC), Notch agonist group (Jagged1) and Jinlong Dingchuan Mixture + Notch agonist group (JLDC + Jagged1) (n=10). The asthma model was prepared by injection and inhalation of ovalbumin (OVA). The mice in each drug group were given the corresponding drug by gavage/intraperitoneally once a day for 14 consecutive days. Resting minute ventilation (VE) and peak expiratory flow rate (PEF) were measured to evaluate the lung function. HE and Masson staining methods were used to observe the pathological injury and fibrosis of lung tissue. The total cell count, eosinophils, neutrophils and lymphocytes were detected in bronchoalveolar lavage fluid (BALF). Flow cytometry was used to determine the proportion of helper T cells 17 (Th17) and regulatory T cells (Treg) in peripheral blood. The levels of interleukin (IL)-10, IL-17 and transforming growth factor-β (TGF-β) in bronchoalveolar lavage fluid (BALF) were measured, and the expressions of Notch1 and Hes1 protein in lung tissue were detected.

Results

The lung function of JLDC group was significantly improved, and the pathological injury and fibrosis of the lung tissue were significantly alleviated. The number of inflammatory cells in BALF decreased significantly. Th17/Treg ratio was significantly decreased, the levels of IL-10 and TGF-β were significantly increased, and IL-17 levels were significantly decreased. The expression levels of Notch1 and Hes1 were significantly decreased. Notch agonist Jagged1 can partially reverse the above therapeutic effects of Jinlong Dingchuan Mixture on the asthmatic mice.

Conclusion

Jinlong Dingchuan Mixture can improve Th17/Treg balance, inhibit inflammatory response and improve lung function in the mice with tracheal asthma, and its mechanism may be related to inhibiting Notch signaling pathway.

Key words

Jinlong Dingchuan Mixture (金龙定喘合剂) / bronchial asthma / Notch signaling pathway / Th17/Treg balance / inflammatory response

引用本文

赵姣, 尹江涛, 董思麟, 张月, 崔英海, 关玉娟, 丁晓欢. 基于Notch信号通路探讨金龙定喘合剂调节免疫失衡治疗哮喘的作用机制. 中华中医药学刊, 2026 , 44 (8) : 36 -40 . DOI: 10.13193/j.issn.1673-7717.2026.08.008

Jiao ZHAO, Jiangtao YIN, Siling DONG, Yue ZHANG, Yinghai CUI, Yujuan GUAN, Xiaohuan DING. Exploring Mechanism of Jinlong Dingchuan Mixture (金龙定喘合剂) Regulating Immune Imbalance in Treating Asthma Based on Notch Signaling Pathway[J]. Chinese Archives of Traditional Chinese Medicine, 2026 , 44 (8) : 36 -40 . DOI: 10.13193/j.issn.1673-7717.2026.08.008

基金

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国家重点研发计划项目(2018YFC1707410)
辽宁省中医药研究院数字化慢阻肺管理系统中西医综合评价及应用推广示范研究项目(CXZH04)
辽宁中医药大学附属第二医院“育苗”项目(2023-LZYY-1-21; 2023-LZYY-1-29; 2023-LZYY-1-17)

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2026年第44卷第8期

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文章信息

doi: 10.13193/j.issn.1673-7717.2026.08.008

首发时间：2026-10-20
出版时间：2026-08-10

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出版历史

基金

国家重点研发计划项目(2018YFC1707410)

辽宁省中医药研究院数字化慢阻肺管理系统中西医综合评价及应用推广示范研究项目(CXZH04)

辽宁中医药大学附属第二医院“育苗”项目(2023-LZYY-1-21; 2023-LZYY-1-29; 2023-LZYY-1-17)

作者信息

^1.辽宁中医药大学附属第二医院，辽宁　沈阳　110034

^2.辽宁中医药大学研究生院，辽宁　沈阳　110847

通讯作者:

关玉娟（1978-），女（满族），辽宁丹东人，主任中医师，博士，研究方向：中医药治疗肺系疾病。E-mail：snowthaw@163.com。

丁晓欢（1982-），女，辽宁沈阳人，副主任中医师，博士，研究方向：中医肺病、中西医结合基础及临床。E-mail：13840185127@126.com。

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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