中华中医药学刊

基于代谢组学研究经方天麻钩藤饮对抽动障碍模型小鼠的保护作用机制

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陈宏 ¹ , 王文增 ² , 李可心 ² , 吴国航 ² , 张伟 ¹ , 冯大庆 ¹ , 于佳琪 ² , 宋倩男 ² , 齐鑫 ²

中华中医药学刊 | 博士导师新论 2026,44(8): 1-5

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中华中医药学刊 |博士导师新论 2026 , 44 (8) : 1 -5

基于代谢组学研究经方天麻钩藤饮对抽动障碍模型小鼠的保护作用机制

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陈宏¹, 王文增², 李可心², 吴国航², 张伟¹, 冯大庆¹, 于佳琪², 宋倩男², 齐鑫²

作者信息

^1.黑龙江中医药大学附属第一医院，黑龙江　哈尔滨　150040

^2.黑龙江中医药大学，黑龙江　哈尔滨　150040

作者简介:

陈宏（1977-），男，黑龙江哈尔滨人，主任医师，博士研究生导师，博士，研究方向：中医药防治小儿心肝系统疾病。

Analysis on Protective Effect and Mechanism of Tianma Gouteng Decoction (天麻钩藤饮) for Tic Disorder Model Mice Based on Metabolomics

Hong CHEN¹, Wenzeng WANG², Kexin LI², Guohang WU², Wei ZHANG¹, Daqing FENG¹, Jiaqi YU², Qiannan SONG², Xin QI²

Affiliations

^1.The First Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin 150040, Heilongjiang, China

^2.Heilongjiang University of Chinese Medicine, Harbin 150040, Heilongjiang, China

出版时间: 2026-08-10 doi: 10.13193/j.issn.1673-7717.2026.08.001

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摘要

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目的

基于超高效液相色谱四极杆飞行时间质谱（UPLC/Q-TOF MS）研究经方天麻钩藤饮对抽动障碍模型小鼠的干预及脑组织中纹状体代谢物的影响，以探究经方天麻钩藤饮治疗抽动障碍的保护机制。

方法

取60只ICR小鼠（雄性），分为6组，每组10只，分别为对照组、模型组、西药组、天麻钩藤饮高剂量组、天麻钩藤饮中剂量组、天麻钩藤饮低剂量组。通过亚氨基二丙腈（Iminodipropionitrile，IDPN）构建小鼠抽动障碍模型，综合小鼠行为学变化和组织病理，结合小鼠脑组织多巴胺（Dopamine，DA）、5-羟色胺（5-Hydroxytryptamine，5-HT）与去甲肾上腺素（Norepinephrine，NE）的含量，初步评价经方天麻钩藤饮对抽动障碍模型小鼠的治疗效果。利用UPLC/Q-TOF MS技术检测各组小鼠脑组织中的纹状体代谢物，以正交偏最小二判别分析进行多元统计分析模式识别，筛选差异代谢物，并借助MetaboAnalyst5.0数据库富集代谢通路。

结果

经方天麻钩藤饮可以显著改善抽动障碍模型小鼠的刻板行为评分，降低肺指数，5-HT检测量下调，DA、NE检测量上升。相比于对照组，模型组小鼠脑组织5-HT检测量显著升高（P<0.05），DA和NE检测量显著降低（P<0.05）；相较于模型组，天麻钩藤饮高、中、低剂量组小鼠脑组织5-HT均下降，DA、NE均上升（P<0.05）；进一步对比可见，天麻钩藤饮高剂量组小鼠脑组织中5-HT含量较其中、低剂量组更低，DA、NE含量则更高（P<0.05）。代谢组学结果显示，与经方天麻钩藤饮调控相关的差异代谢物共有50余个，包含甘油磷脂、亚油酸、花生四烯酸等物质。

结论

从代谢组学角度分析了经方天麻钩藤饮治疗抽动障碍的作用机制，为经方天麻钩藤饮治疗抽动性障碍症研究及临床应用奠定了基础。

关键词

代谢组学 / 经方天麻钩藤饮 / 抽动障碍 / 小鼠 / 作用机制

Abstract

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Objective

This study aimed to investigate the mechanism of Tianma Gouteng Decoction (天麻钩藤饮) in the treatment of tic disorders by examining its intervention effects on tic disorder model mice and its impact on striatal metabolites in brain tissue using ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF MS).

Methods

Sixty male ICR mice were chosen and randomly allocated into 6 groups with 10 mice in each, specifically the control group, the model group, the western medicine group, as well as the Tianma Gouteng Decoction high, medium and low dose groups. A tic disorder model in mice was constructed via intraperitoneal injection of iminodipropionitrile (IDPN). The therapeutic effect of Tianma Gouteng Decoction on tic disorder model mice was preliminarily evaluated by comprehensively considering changes in mouse behavior, histopathology, as well as the levels of dopamine (DA), 5-hydroxytryptamine (5-HT) and norepinephrine (NE) in the brain tissue of mice. UPLC/Q-TOF MS was used to detect striatal metabolites in the brain tissue of the mice in the control group, the model group, the western medicine group and Tianma Gouteng Decoction high, medium and low dose groups. Orthogonal partial least squares-discriminant analysis was adopted for multivariate statistical analysis and pattern recognition, aiming to screen out differential metabolites. Metabolic pathway enrichment was performed using the MetaboAnalyst 5.0 database.

Results

Tianma Gouteng Decoction could significantly improve the stereotyped behavior scores of tic disorder model mice, reduce the lung index, lower the level of 5-HT and raise the levels of DA and NE. In particular, when compared with those in the control group, the level of 5-HT in the brain tissue of the mice in the model group was significantly higher (P<0.05), whereas the levels of DA and NE were significantly reduced (P<0.05). In comparison with the model group, the 5-HT levels in the brain tissue of the mice in the Tianma Gouteng Decoction high, medium and low dose groups were significantly lowered, while the levels of DA and NE were significantly elevated (P<0.05). Further comparison showed that the content of 5-HT in the brain tissue of the mice in the Tianma Gouteng Decoction high-dose group was lower than that in the Tianma Gouteng Decoction medium and low dose groups, while the contents of DA and NE were higher (P<0.05). Metabolomics results revealed that there were more than 50 differential metabolites related to the regulation of Tianma Gouteng Decoction, including glycerophospholipids, linoleic acid and arachidonic acid.

Conclusion

From a metabolomics standpoint, this research explored the therapeutic mechanism of Tianma Gouteng Decoction in addressing tic disorders, laying a foundation for research and clinical application of Tianma Gouteng Decoction in the treatment of tic disorders.

Key words

metabolomics / Jingfang Tianma Gouteng Decoction (天麻钩藤饮) / tic disorder model / mice / action mechanism

引用本文

陈宏, 王文增, 李可心, 吴国航, 张伟, 冯大庆, 于佳琪, 宋倩男, 齐鑫. 基于代谢组学研究经方天麻钩藤饮对抽动障碍模型小鼠的保护作用机制. 中华中医药学刊, 2026 , 44 (8) : 1 -5 . DOI: 10.13193/j.issn.1673-7717.2026.08.001

Hong CHEN, Wenzeng WANG, Kexin LI, Guohang WU, Wei ZHANG, Daqing FENG, Jiaqi YU, Qiannan SONG, Xin QI. Analysis on Protective Effect and Mechanism of Tianma Gouteng Decoction (天麻钩藤饮) for Tic Disorder Model Mice Based on Metabolomics[J]. Chinese Archives of Traditional Chinese Medicine, 2026 , 44 (8) : 1 -5 . DOI: 10.13193/j.issn.1673-7717.2026.08.001

基金

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中国博士后科学基金第13批特别资助项目(2020T130178)
黑龙江省自然科学基金项目(LH2023H074)
黑龙江省卫生健康委员会项目(20230202040115)
黑龙江省中医药科研项目(ZHY2024-047)

参考文献

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2026年第44卷第8期

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doi: 10.13193/j.issn.1673-7717.2026.08.001

首发时间：2026-10-20
出版时间：2026-08-10

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基金

中国博士后科学基金第13批特别资助项目(2020T130178)

黑龙江省自然科学基金项目(LH2023H074)

黑龙江省卫生健康委员会项目(20230202040115)

黑龙江省中医药科研项目(ZHY2024-047)

作者信息

^1.黑龙江中医药大学附属第一医院，黑龙江　哈尔滨　150040

^2.黑龙江中医药大学，黑龙江　哈尔滨　150040

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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