中华中医药学刊

生地黄中梓醇和地黄苷A通过激活胰岛β细胞自噬抑制凋亡发挥抗2型糖尿病作用

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胡楠 ¹ , 孟祥龙 ¹^,²^,³^,⁴ , 李鸿昌 ² , 张朔生 ¹^,²^,³^,⁴

中华中医药学刊 | 国家项目点击 2026,44(8): 111-115

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中华中医药学刊 |国家项目点击 2026 , 44 (8) : 111 -115

生地黄中梓醇和地黄苷A通过激活胰岛β细胞自噬抑制凋亡发挥抗2型糖尿病作用

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胡楠¹, 孟祥龙¹^,²^,³^,⁴, 李鸿昌², 张朔生¹^,²^,³^,⁴

作者信息

^1.山西中医药大学中药与食品工程学院，山西　晋中　030619

^2.山西中医药大学中药炮制山西省重点实验室，山西　晋中　030619

^3.山西中医药大学中药材加工炮制传承与创新重点研究室，山西　晋中　030619

^4.中药炮制技术传承基地（山西中医药大学），山西　晋中　030619

通讯作者:

孟祥龙（1988-），男，黑龙江铁力人，高级实验师，硕士研究生导师，博士，研究方向：中药炮制现代化。E-mail：sszywzh@126.com。

作者简介:

胡楠（1990-），女，山西临汾人，实验师，硕士，研究方向：中药药效分子机制。

Catalpol and Rehmannioside A from Shengdiihuang (Rehmanniae Radix) Activate Autophagy And Inhibit Apoptosis In Pancreatic β-Cells To exert anti-T2DM Effects

Nan HU¹, Xianglong MENG¹^,²^,³^,⁴, Hongchang LI², Shuosheng ZHANG¹^,²^,³^,⁴

Affiliations

^1.Traditional Chinese Medicine and Food Engineering, Shanxi University of Chinese Medicine, Jinzhong 030619, Shanxi, China

^2.Shanxi Key Laboratory of Chinese Herbal Medicine Processing, Shanxi University of Chinese Medicine, Jinzhong 030619, Shanxi, China

^3.Key Research Laboratory of Processing and Innovation in Traditional Chinese Medicinal Materials, Shanxi University of Chinese Medicine, Jinzhong 030619, Shanxi, China

^4.Traditional Chinese Medicine Processing Technology Inheritance Base (Shanxi University of Chinese Medicine), Jinzhong 030619, Shanxi, China

出版时间: 2026-08-10 doi: 10.13193/j.issn.1673-7717.2026.08.024

文章导航

摘要

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目的

探讨中药地黄的两种环烯醚萜类成分梓醇（Catalpol）及地黄苷A（Rehmannioside A，ReA）对过氧化氢（H₂O₂）诱导胰岛β细胞损伤的保护作用，以及在治疗2型糖尿病（type 2 diabetes mellitus，T2DM）中的分子机制。

方法

通过应用H₂O₂诱导大鼠胰岛细胞瘤细胞（Rat insulinoma cells，INS-1）及小鼠胰岛β细胞（Mouse pancreatic β cells，MIN6）损伤模型，评价梓醇和地黄苷A对细胞活力及增殖的影响，并结合流式细胞术、细胞免疫荧光、蛋白免疫印迹等方法，检测细胞自噬、凋亡及相关蛋白表达。

结果

梓醇和地黄苷A能够明显提升H₂O₂诱导的INS-1及MIN6细胞存活率（P<0.01）；显著提升细胞的增殖活力（P<0.01）；有效降低细胞的凋亡率（P<0.01）；细胞中微管相关蛋白轻链3（Microtubule-associated protein light chain 3，LC3）蛋白相对荧光强度显著增强（P<0.01）；上调细胞Bcl-2同源结构域蛋白1（Beclin1）、LC3B Ⅱ、自噬相关蛋白7（Autophagy related 7，ATG7）、自噬相关蛋白5（Autophagy related 5，ATG5）、B淋巴细胞瘤-2蛋白（B-cell lymphoma-2，Bcl-2）、半胱天冬酶-9（Cysteinyl aspartate specific proteinase-9，Caspase-9）、半胱天冬酶-3（Cysteinyl aspartate specific proteinase-3，Caspase-3）、聚腺苷二磷酸核糖聚合酶1（Poly ADP-ribose polymerase 1，PARP1）蛋白表达（P<0.05，P<0.01）；下调细胞选择性自噬接头蛋白（Sequestosome 1，P62）、Bcl-2相关X蛋白（Bcl-2 associated X protein，Bax）蛋白表达（P<0.05，P<0.01）。

结论

梓醇和地黄苷A通过增强胰岛β细胞自噬活性，减少因氧化损伤导致的细胞凋亡，改善胰岛β细胞的活力与增殖，最终发挥抗T2DM的作用。

关键词

梓醇 / 地黄苷A / 胰岛β细胞 / 细胞自噬 / 细胞凋亡

Abstract

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Objective

Explored the protective effects of two iridoid compounds, catalpol and rehmannioside A (ReA), from the traditional Chinese medicine Shengdiihuang (Rehmanniae Radix), against hydrogen peroxide (H₂O₂)-induced injury of pancreatic β-cells in vitro, as well as their molecular mechanisms in the treatment of type 2 diabetes mellitus (T2DM).

Methods

By utilizing an H₂O₂-induced injury model of rat insulinoma cells (INS-1) and mouse pancreatic β-cells (MIN6), the effects of catalpol and ReA on cell viability and proliferation were evaluated. Additionally, flow cytometry, cellular immunofluorescence and protein immunoblotting were employed to detect autophagy, apoptosis and the expressions of related proteins.

Results

Catalpol and ReA can significantly increase the survival rate of H₂O₂-induced INS-1 and MIN6 cells (P<0.01), markedly enhance the cell proliferation vitality (P<0.01), effectively reduce the apoptosis rate of cells (P<0.01), significantly increase the relative fluorescence intensity of Microtubule-associated protein light chain 3 (LC3) protein in cells (P<0.01), up-regulate the expressions of Beclin 1, LC3B Ⅱ, Autophagy related 7 (ATG7), Autophagy related 5 (ATG5), B-cell lymphoma-2 (Bcl-2), Cysteinyl aspartate specific proteinase-9 (Caspase-9), Cysteinyl aspartate specific proteinase-3 (Caspase-3), Poly ADP-ribose polymerase 1 (PARP1) proteins in cells (P<0.05, P<0.01) and down-regulate the expressions of Sequestosome 1 (P62) and Bcl-2 associated X protein (Bax) proteins in cells (P<0.05, P<0.01).

Conclusion

Catalpol and ReA exert their anti-T2DM effects by enhancing autophagic activity, reducing cell apoptosis caused by oxidative damage, and improving the viability and proliferation of pancreatic β-cells.

Key words

catalpol / rehmannioside A / pancreatic β-cells / autophagy / apoptosis

引用本文

胡楠, 孟祥龙, 李鸿昌, 张朔生. 生地黄中梓醇和地黄苷A通过激活胰岛β细胞自噬抑制凋亡发挥抗2型糖尿病作用. 中华中医药学刊, 2026 , 44 (8) : 111 -115 . DOI: 10.13193/j.issn.1673-7717.2026.08.024

Nan HU, Xianglong MENG, Hongchang LI, Shuosheng ZHANG. Catalpol and Rehmannioside A from Shengdiihuang (Rehmanniae Radix) Activate Autophagy And Inhibit Apoptosis In Pancreatic β-Cells To exert anti-T2DM Effects[J]. Chinese Archives of Traditional Chinese Medicine, 2026 , 44 (8) : 111 -115 . DOI: 10.13193/j.issn.1673-7717.2026.08.024

基金

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国家自然科学基金面上项目(81673601)
国家中医药管理局中药炮制技术传承基地项目（2022年）
山西省应用基础研究计划项目(20210302124694)
山西省教育厅项目(2021L364; 2023L189)
山西省中医药管理局科研项目(2023ZYYA2012)
山西省留学人员科技活动择优项目（重点项目）(20230034)
山西省筹资金资助归国留学人员科研项目(2023-156)
山西中医药大学博士科研启动基金项目(2023BK38)
山西中医药大学科技创新能力培育计划（太行本草专项）项目(2022PY-TH-02)
山西中医药大学基础与临床合作专项(2024PY-JL-7-02)

参考文献

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2026年第44卷第8期

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文章信息

doi: 10.13193/j.issn.1673-7717.2026.08.024

首发时间：2026-10-20
出版时间：2026-08-10

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基金

国家自然科学基金面上项目(81673601)

国家中医药管理局中药炮制技术传承基地项目（2022年）

山西省应用基础研究计划项目(20210302124694)

山西省教育厅项目(2021L364; 2023L189)

山西省中医药管理局科研项目(2023ZYYA2012)

山西省留学人员科技活动择优项目（重点项目）(20230034)

山西省筹资金资助归国留学人员科研项目(2023-156)

山西中医药大学博士科研启动基金项目(2023BK38)

山西中医药大学科技创新能力培育计划（太行本草专项）项目(2022PY-TH-02)

山西中医药大学基础与临床合作专项(2024PY-JL-7-02)

作者信息

^1.山西中医药大学中药与食品工程学院，山西　晋中　030619

^2.山西中医药大学中药炮制山西省重点实验室，山西　晋中　030619

^3.山西中医药大学中药材加工炮制传承与创新重点研究室，山西　晋中　030619

^4.中药炮制技术传承基地（山西中医药大学），山西　晋中　030619

通讯作者:

孟祥龙（1988-），男，黑龙江铁力人，高级实验师，硕士研究生导师，博士，研究方向：中药炮制现代化。E-mail：sszywzh@126.com。

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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