中华中医药学刊

探明不同浓度淫羊藿对大鼠脂肪干细胞成骨分化的影响

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舒雷 , 廖冰 , 刘海全

中华中医药学刊 | 国家项目点击 2026,44(9): 32-36

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中华中医药学刊 |国家项目点击 2026 , 44 (9) : 32 -36

探明不同浓度淫羊藿对大鼠脂肪干细胞成骨分化的影响

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舒雷, 廖冰, 刘海全

作者信息

广州中医药大学惠州医院（惠州市中医医院），广东　惠州　516001

通讯作者:

刘海全（1977-），男，河北滦县人，主任中医师，博士研究生导师，博士，研究方向：中医药防治骨质疏松。E-mail：liuhaiq1977@163.com。

作者简介:

舒雷（1998-），男，湖北荆州人，硕士在读，研究方向：中医药防治骨质疏松症。

Effects of Different Concentrations of Yinyanghuo (Epimedii Folium) Extract on Osteogenic Differentiation of Rats Adipose-Derived Stem Cells

Lei SHU, Bing LIAO, Haiquan LIU

Affiliations

Huizhou Hospital of Guangzhou University of Chinese Medicine (Huizhou Hospital ofTraditional Chinese Medicine), Huizhou 516001, Guangdong, China

出版时间: 2026-09-10 doi: 10.13193/j.issn.1673-7717.2026.09.006

文章导航

摘要

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目的

探明淫羊藿醇提物对大鼠脂肪干细胞（adipose-derived stem cells，ADSCs）成骨分化的影响。

方法

取5只健康未生育SPF级SD雌性大鼠进行分离、培养脂肪干细胞，获得第3代ADSCs用茜素红和油红O染色，用1、10、50和100 μmol/L淫羊藿醇提物培养，评价其成骨和成脂分化能力。将第3代ADSCs分为空白组、白藜芦醇组（100 μmol/L）、淫羊藿醇提物Ⅰ～Ⅳ组（1、10、50、100 μmol/L），培养7 d后对脂肪干细胞LIM结构域蛋白Ajuba（LIM domain-containing protein ajuba，Ajuba）、Runt相关转录因子2（Runx-related transcription factor 2，Runx2）、碱性磷酸酶（Alkaline phosphatase，ALP）表达水平进行qRT-PCR、Western-blotting检测。

结果

白藜芦醇组和不同浓度（1、10、50、100 μmol/L）淫羊藿醇提物组均能促进Runx2基因和蛋白、ALP的基因表达水平（P<0.01）；且与白藜芦醇组比较，100 μmol/L淫羊藿醇提物组显示出更强的促进作用（P<0.01）。在ALP蛋白的表达水平方面，白藜芦醇组的促进作用优于淫羊藿I～IV组（P<0.01）。白藜芦醇组和不同浓度（1、10、50、100 μmol/L）淫羊藿醇提物组均会抑制Ajuba基因表达（P<0.01，P<0.05），其中白藜芦醇组在相同浓度下表现出较强的基因抑制作用。

结论

淫羊藿醇提物与白藜芦醇均能促进脂肪干细胞成骨分化，但淫羊藿醇提物的促进作用更明显，效果更显著，两者均能抑制成脂分化，而在短期内白藜芦醇抑制成脂分化更优。随着干预的时间延长，随后的趋势有待进一步研究。

关键词

淫羊藿醇提物 / 脂肪干细胞 / 白藜芦醇 / 成骨分化 / 成脂分化

Abstract

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Objective

To investigate the effects of different concentrations of Yinyanghuo (Epimedii Folium) extract on osteogenic differentiation of the rats adipose-derived stem cells (ADSCs).

Methods

Five healthy, nulliparous SPF-grade female SD rats were used to isolate and culture the ADSCs. The obtained third-generation ADSCs were stained with Alizarin Red and Oil Red O to evaluate their osteogenic and adipogenic differentiation abilities after being cultured with 1, 10, 50, 100 μmol/L Yinyanghuo (Epimedii Folium) extract. Then, the third-generation ADSCs were divided into a blank group, a resveratrol group (100 μmol/L) and Yinyanghuo (Epimedii Folium) extract groups. According to the different concentrations of Yinyanghuo (Epimedii Folium) extract, four subgroups were created, namely Ⅰ～Ⅳ groups (1, 10, 50, 100 μmol/L). After 7 days of culture, qRT-PCR and Western blotting were performed to detect the expression levels of LIM domain-containing protein Ajuba (Ajuba), Runx-related transcription factor 2 (Runx2), alkaline phosphatase (ALP) in the ADSCs.

Results

The resveratrol group and Yinyanghuo (Epimedii Folium) extract I to IV groups all upregulated the expression levels of Runx2 gene and protein, as well as ALP gene, with the best effect observed in the 100 μmol/L Yinyanghuo (Epimedii Folium) extract group (P<0.01). As for the expression levels of ALP protein, the resveratrol group exhibited a stronger promoting effect than the Yinyanghuo (Epimedii Folium) extract I to IV groups (P<0.01). The resveratrol group and Yinyanghuo (Epimedii Folium) extract groups I to IV all downregulated the expression levels of Ajuba gene and protein (P<0.01, P<0.05), with the resveratrol group showing a stronger gene inhibitory effect at the same concentration.

Conclusion

Both Yinyanghuo (Epimedii Folium) extract and resveratrol can promote the osteogenic differentiation of adipose-derived stem cells, but the promoting effect of Yinyanghuo (Epimedii Folium) extract is more significant and effective. Both can also inhibit adipogenic differentiation, with Yinyanghuo (Epimedii Folium) extract exhibiting weaker inhibition than resveratrol in the short term. With the extension of intervention time, further research is needed to investigate the trend in the future.

Key words

Yinyanghuo (Epimedii Folium) extract / adipose-derived stem cells / resveratrol / osteogenic differentiation / adipogenic differentiation

引用本文

舒雷, 廖冰, 刘海全. 探明不同浓度淫羊藿对大鼠脂肪干细胞成骨分化的影响. 中华中医药学刊, 2026 , 44 (9) : 32 -36 . DOI: 10.13193/j.issn.1673-7717.2026.09.006

Lei SHU, Bing LIAO, Haiquan LIU. Effects of Different Concentrations of Yinyanghuo (Epimedii Folium) Extract on Osteogenic Differentiation of Rats Adipose-Derived Stem Cells[J]. Chinese Archives of Traditional Chinese Medicine, 2026 , 44 (9) : 32 -36 . DOI: 10.13193/j.issn.1673-7717.2026.09.006

基金

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国家自然科学基金项目(82174147)
广东省基础与应用基础研究基金项目(2021A1515011271)
广东省中医药局科研项目(20213019)

参考文献

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排序方式：

[1]

中国骨质疏松症流行病学调查及“健康骨骼”专项行动结果发布[J]. 中华骨质疏松和骨矿盐疾病杂志, 2019, 12(4): 317-318.

[2]

陈菊芳, 田玉楼, 郝鑫. 脂肪干细胞在颅颌面骨再生中的作用与潜力[J]. 中国组织工程研究, 2021, 25(13): 2087-2096.

[3]

STORTI G, SCIOLI M G, KIM B S, et al. Adipose-derived stem cells in bone tissue engineering: useful tools with new applications[J]. Stem Cells Int, 2019, 2019: 3673857.

[4]

MA H P, HE X R, YANG Y, et al. The genus Epimedium: an ethnopharmacological and phytochemical review[J]. J Ethnopharmacol, 2011, 134(3): 519-541.

[5]

张锦明, 田滢舟, 赵玲, 等. 淫羊藿苷促进骨髓间充质干细胞成骨分化缓解小鼠骨质疏松症的机制[J]. 中国组织工程研究, 2022, 26(19): 2991-2996.

[6]

宁兆荣, 郭延伟, 李松, 等. 脂肪干细胞复合载淫羊藿苷支架材料对兔下颌骨缺损的修复[J]. 实用口腔医学杂志, 2013, 29(5): 611-615.

[7]

刘海全, 秦佳佳. 淫羊藿含药血清对骨质疏松大鼠骨髓基质干细胞成脂分化PPARγ mRNA表达的影响[J]. 北京中医药大学学报, 2012, 35(9): 611-614.

[8]

周爱珍, 王蕾, 程斌. 淫羊藿醇提工艺及醇提物对软骨细胞保护作用的研究[J]. 中国中药杂志, 2020, 45(5): 1097-1104.

[9]

邱俊杰, 王伟舟, 赵泽玉, 等. 白藜芦醇在绝经后骨质疏松症中抗氧化机制的研究进展[J]. 中国骨质疏松杂志, 2020, 26(9): 1370-1374.

[10]

刘洪江, 刘海全, 秦佳佳. 不同浓度淫羊藿含药血清干预骨髓基质干细胞的成脂分化[J]. 中国组织工程研究与临床康复, 2011, 15(14): 2583-2586.

[11]

迟利业, 原超, 卢义, 等. 淫羊藿含药血清对BMSCs中Sirt1mRNA的表达的影响[J]. 时珍国医国药, 2018, 29(11): 2575-2577.

[12]

吴启弘, 张永祥, 余江, 等. 淫羊藿苷对骨折大鼠骨髓基质干细胞增殖及成骨分化的影响[J]. 中国医药导报, 2024, 21(7): 23-26.

[13]

胡少华, 赵振理, 万智盛, 等. 淫羊藿总黄酮对兔脂肪干细胞向尿路上皮细胞增殖分化的影响[J]. 临床医药实践, 2023, 32(11): 828-833.

[14]

蔡叶, 王明飞, 张磊, 等. 淫羊藿苷对衰老骨髓间充质干细胞成骨的影响[J]. 中国骨质疏松杂志, 2023, 29(2): 162-167.

[15]

吴梅剑宗, 秦佳佳, 刘海全. 不同浓度淫羊藿苷对大鼠脂肪干细胞成骨分化相关因子的体外研究[J]. 北京中医药大学学报, 2022, 45(12): 1249-1256.

[16]

DONG Y M, KANG H L, PENG R P, et al. Global, regional, and national burden of low bone mineral density from 1990 to 2019: results from the global burden of disease study 2019[J]. Front Endocrinol, 2022, 13: 870905.

[17]

JEREMIAH M P, UNWIN B K, GREENAWALD M H, et al. Diagnosis and management of osteoporosis[J]. Am Fam Physician, 2015, 92(4): 261-268.

[18]

ZHU J, MARCH L. Treating osteoporosis: risks and management[J]. Aust Prescr, 2022, 45(5): 150-157.

[19]

PENG Z C, XU R H, YOU Q J. Role of traditional Chinese medicine in bone regeneration and osteoporosis[J]. Front Bioeng Biotechnol, 2022, 10: 911326.

[20]

ZHAO H Y, ZHAO N, ZHENG P, et al. Prevention and treatment of osteoporosis using Chinese medicinal plants: special emphasis on mechanisms of immune modulation[J]. J Immunol Res, 2018, 2018: 6345857.

[21]

ZHANG N D, HAN T, HUANG B K, et al. Traditional Chinese medicine formulas for the treatment of osteoporosis: Implication for antiosteoporotic drug discovery[J]. J Ethnopharmacol, 2016, 189: 61-80.

[22]

马震东, 黄淑芸, 黄一兴, 等. 淫羊藿治疗骨质疏松的网络药理学及分子对接技术研究[J]. 牡丹江医学院学报, 2022, 43(5): 35-40, 59.

[23]

YANG A F, YU C C, LU Q L, et al. Mechanism of action of icariin in bone marrow mesenchymal stem cells[J]. Stem Cells Int, 2019, 2019: 5747298.

[24]

LEE S, CHAE D S, SONG B W, et al. ADSC-based cell therapies for musculoskeletal disorders: a review of recent clinical trials[J]. Int J Mol Sci, 2021, 22(19): 253-256.

[25]

LI J, XIN Z X, CAI M J. The role of resveratrol in bone marrow-derived mesenchymal stem cells from patients with osteoporosis[J]. J Cell Biochem, 2019, 120(10): 16634-16642.

[26]

唐晓悦, 朱浩, 曹灿. 白藜芦醇通过沉默信息调节因子/叉头转录因子通路对高糖诱导成骨细胞增殖、分化的影响[J]. 口腔医学研究, 2020, 36(1): 79-84.

[27]

张浩, 郑玉玲, 丁辉. 淫羊藿苷治疗骨质疏松的相关通路研究进展[J]. 天津中医药大学学报, 2022, 41(4): 531-538.

[28]

LI Q, PENG H, FAN H, et al. The LIM protein Ajuba promotes adipogenesis by enhancing PPARγ and p300/CBP interaction[J]. Cell Death Differ, 2016, 23(1): 158-168.

2026年第44卷第9期

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文章信息

doi: 10.13193/j.issn.1673-7717.2026.09.006

首发时间：2026-10-20
出版时间：2026-09-10

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基金

国家自然科学基金项目(82174147)

广东省基础与应用基础研究基金项目(2021A1515011271)

广东省中医药局科研项目(20213019)

作者信息

广州中医药大学惠州医院（惠州市中医医院），广东　惠州　516001

通讯作者:

刘海全（1977-），男，河北滦县人，主任中医师，博士研究生导师，博士，研究方向：中医药防治骨质疏松。E-mail：liuhaiq1977@163.com。

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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