Article(id=1317125548661388268, tenantId=1146029695717560320, journalId=1255847919539208197, issueId=1317125500041015883, articleNumber=null, orderNo=null, doi=10.13193/j.issn.1673-7717.2026.02.027, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=null, receivedDateStr=null, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1791956801810, onlineDateStr=2026-10-14, pubDate=1770652800000, pubDateStr=2026-02-10, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1791956801810, onlineIssueDateStr=2026-10-14, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1791956801810, creator=13701087609, updateTime=1791956801810, updator=13701087609, issue=Issue{id=1317125500041015883, tenantId=1146029695717560320, journalId=1255847919539208197, year='2026', volume='44', issue='2', pageStart='1', pageEnd='258', issueExtLink='null', onlineDate='null', pubDate='1770652800000', pubDateStr='2026-02-10', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1791956790217, creator='13701087609', updateTime=1791958795145, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1317133909368877755, tenantId=1146029695717560320, journalId=1255847919539208197, issueId=1317125500041015883, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1317133909373072060, tenantId=1146029695717560320, journalId=1255847919539208197, issueId=1317125500041015883, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=148, endPage=155, ext={EN=ArticleExt(id=1317125548858520557, articleId=1317125548661388268, tenantId=1146029695717560320, journalId=1255847919539208197, language=EN, title=Differential Compounds Between Fresh and Dry Donglingcao［Rabdosia rubescens（Hemsl.）Hara］ Based on UHPLC-Q-Exactive HF-X Combined with Multivariate Statistical Analysis, columnId=1256183361521967297, journalTitle=Chinese Archives of Traditional Chinese Medicine, columnName=Target on National Project, runingTitle=null, highlight=null, articleAbstract=
Objective To investigate the composition differences between the fresh Donglingcao［Rabdosia rubescens（Hemsl.）Hara］（FRH）and its two dry products（natural drying and vacuum freeze-drying）.
Method Ultra-High Performance Liquid Chromatography-Quadrupole-Exactive Mass Spectrometry（UHPLC-Q-Exactive HF-X/MS）was used to collect MS data from Donglingcao［Rabdosia rubescens（Hemsl.）Hara］ compounds in positive and negative ion modes. The components were identified and analyzed based on the online database，local standard product database and literature. Then，principal component analysis（PCA）and orthogonal partial least squares-discriminant analysis（OPLS-DA）were performed on the collected data through SIMCA software. The differential components between fresh and dried products were screened out based on variable importance in projection（VIP）and t-test. Finally，the relative quantitative analysis was carried out according to peak areas of differential components.
Result A total of 61 compounds were identified，comprising 31 diterpenoids，12 phenolic acids，9 flavonoids，4 triterpenes and 5 other compounds. PCA intuitively showed that the metabolic profile of FRH and its dry products were remarkably different. Thirty-two differential components with VIP>1 and P<0.05 were screened out through OPLS-DA. The contents of the main active ingredients in the FRH such as oridonin and ponicidin were significantly higher in comparison to those in the dry products.
Conclusion In this study，UHPLC-Q-Exactive HF-X/MS combined with multivariate statistical analysis were employed to elucidate the differential components between FRH and its dry products，providing data reference for the rational development and application of Rabdosia rubescens（Hemsl.）Hara.
, authors=Jinying ZHANG
1, Shuo TIAN
1, 2, Lin GUO
1, Zhiguo MAO
1, Yilong HU
1, Mingsan MIAO
1, 2, authorsList=Jinying ZHANG, Shuo TIAN, Lin GUO, Zhiguo MAO, Yilong HU, Mingsan MIAO, authorCompany=null, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1317125549156316142, articleId=1317125548661388268, tenantId=1146029695717560320, journalId=1255847919539208197, language=CN, title=基于UHPLC-Q-Exactive HF-X结合多元统计分析探究鲜、干冬凌草化学成分差异, columnId=1256183362172142382, journalTitle=中华中医药学刊, columnName=国家项目点击, runingTitle=null, highlight=null, articleAbstract=
目的 探究鲜冬凌草与其干品（自然晒干、真空冷冻干燥）的化学成分差异。
方法 利用超高效液相-四极杆-静电轨道阱高分辨质谱（Ultra-high performance liquid chromatography-quadrupole-exactive mass spectrometry，UHPLC-Q-Exactive HF-X/MS）联用技术在正、负离子模式下采集冬凌草化学成分质谱数据，并基于在线数据库、本地自建标准品数据库及文献报道对其成分进行鉴定分析；通过SIMCA软件对采集的数据进行主成分分析（Principal component analysis，PCA）和正交偏最小二乘法判别分析（Orthogonal partial least squares-discriminant analysis，OPLS-DA），根据获得的变量投影重要性（Variable importance in projection，VIP）和t检验筛选鲜干冬凌草间的差异化学成分；最后，根据差异成分离子峰面积进行相对定量分析。
结果 共鉴定出61个化学成分，包括二萜类31个、酚酸类12个、黄酮类9个、二萜类4个、三萜类4个、其他类5个；PCA图直观地显示冬凌草鲜品化学轮廓与其干品有明显的差异；通过OPLS-DA筛选出VIP>1和P<0.05的差异化学成分32个，其中差异成分冬凌草甲素、冬凌草乙素等二萜类活性成分在鲜品中相对含量显著高于其干品。
结论 UHPLC-Q-Exactive HF-X/MS结合多元统计分析可用于明确鲜干冬凌草的化学成分差异，进而为鲜干冬凌草资源的合理开发应用提供重要的数据参考。
, authors=张金英
1, 田硕
1, 2, 郭琳
1, 茆志国
1, 胡毅龙
1, 苗明三
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