Article(id=1317125501030875341, tenantId=1146029695717560320, journalId=1255847919539208197, issueId=1317125400581485316, articleNumber=null, orderNo=null, doi=10.13193/j.issn.1673-7717.2026.03.039, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=null, receivedDateStr=null, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1791956790454, onlineDateStr=2026-10-14, pubDate=1773072000000, pubDateStr=2026-03-10, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1791956790454, onlineIssueDateStr=2026-10-14, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1791956790454, creator=13701087609, updateTime=1791956790454, updator=13701087609, issue=Issue{id=1317125400581485316, tenantId=1146029695717560320, journalId=1255847919539208197, year='2026', volume='44', issue='3', pageStart='1', pageEnd='258', issueExtLink='null', onlineDate='null', pubDate='1773072000000', pubDateStr='2026-03-10', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1791956766504, creator='13701087609', updateTime=1791958763799, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1317133777898418844, tenantId=1146029695717560320, journalId=1255847919539208197, issueId=1317125400581485316, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1317133777898418845, tenantId=1146029695717560320, journalId=1255847919539208197, issueId=1317125400581485316, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=210, endPage=213, ext={EN=ArticleExt(id=1317125501215424718, articleId=1317125501030875341, tenantId=1146029695717560320, journalId=1255847919539208197, language=EN, title=Effects of Electroacupuncture on Myocardial Energy Metabolism in Spleen Qi Deficiency Rats Based on PI3K/AKT/GLUT4 Pathway, columnId=1256183365644968140, journalTitle=Chinese Archives of Traditional Chinese Medicine, columnName=Platform of Provincial Project, runingTitle=null, highlight=null, articleAbstract=

The effects of electroacupuncture at “Zulanli（ST36）” on the energy metabolism of myocardial tissue in rats with spleen Qi deficiency were observed, and the intervention mechanism was investigated. Sixty SD rats were randomly divided into blank control group, model group, acupuncture group and non-meridian and non-acupuncture group. The rat model of spleen Qi deficiency was established by the composite factor of “excessive labour and dietary disorders”, the blank control group was raised normally without intervention, the acupuncture group was given electroacupuncture intervention at bilateral “Zulanli（ST36）”, and the non-meridian non-acupuncture group was given non-meridian non-acupuncture acupoints to perform the electroacupuncture control, once a day for 14 d. The general state, body mass and grip strength of the rats were observed at the end of the intervention. The content of adenosine triphosphate（ATP）in myocardial tissue was measured by enzyme-linked immunosorbent assay（ELISA）. The expression levels of glucose transportor（GLUT4）and glucose transportor（GLUT1）in myocardial tissues were detected by immunofluorescence（IF）method. The expression levels of insulin receptor substrate-1（IRS1）, phosphoinosmode-3-kinase（PI3K）, protein kinase B（AKT）and GLUT4 were detected by immunohistochemistry（IHC）method and quantitative real-time fluorescence PCR（RT-qPCR）. Western blot（WB）method was used to detect the protein expression levels of PI3K, AKT and GLUT4 in myocardial tissues. The results showed that compared with those in the blank control group, the rats in the model group and the non-meridian and non-acupoint group were in poorer general condition, in the same trend as the decrease in body weight and grip strength. The ATP production in myocardial tissue was significantly reduced（P < 0.05）, and the protein expression levels and mRNA levels of IRS1, PI3K, AKT, GLUT4 and GLUT1 significantly decreased（P < 0.05）. The rats in the acupuncture group had good general condition, body mass and grip strength, and significantly decreased ATP production in myocardial tissues（P < 0.05）, compared with those in the model group. Compared with those in the model group, the rats in the acupuncture group were in good condition, body mass and grip strength increased, ATP production in myocardial tissues increased significantly（P < 0.05）, and protein expression levels and mRNA levels of IRS1, PI3K, AKT, GLUT4 and GLUT1 increased significantly（P < 0.05）. The mechanism by which electroacupuncture at “Zulanli（ST36）” improved the cardiac energy metabolism in rats with spleen Qi deficiency may be related to the activation of PI3K/AKT/GLUT4 signalling pathway and the enhancement of glucose transport function in myocardial tissues.

, authors=Jidong LIU¹, Yi QU², Lu JIN¹, Jianbo WANG², Yanan XUE¹, Xihan WANG¹, authorsList=Jidong LIU, Yi QU, Lu JIN, Jianbo WANG, Yanan XUE, Xihan WANG, authorCompany=null, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1317125501567746255, articleId=1317125501030875341, tenantId=1146029695717560320, journalId=1255847919539208197, language=CN, title=基于PI3K/AKT/GLUT4通路探讨电针对脾气虚大鼠心肌能量代谢的影响, columnId=1256183369033965799, journalTitle=中华中医药学刊, columnName=省级项目平台, runingTitle=null, highlight=null, articleAbstract=

观察电针“足三里”对脾气虚大鼠心肌组织能量代谢的影响，并探讨其干预机制。60只SD大鼠随机分为空白对照组、模型组、针刺组和非经非穴组。采用“劳倦过度、饮食不节”的复合因素建立脾气虚大鼠模型，空白对照组正常饲养，不予干预，针刺组给予“足三里”穴位电针干预，非经非穴组给予非经非穴点进行电针对照，每日1次，干预14 d。干预结束后观察大鼠的一般状态、体质量和抓力情况；酶联免疫吸附实验（ELISA）法测定心肌组织中三磷酸腺苷（adenosine triphosphate，ATP）含量；免疫荧光（immunofluorescence，IF）法检测心肌组织葡萄糖转运蛋白4（glucose transportor，GLUT4）、葡萄糖转运蛋白1（glucose transportor，GLUT1）表达水平；免疫组化（immunohistochemistry，IHC）法、实时荧光定量（Quantitative Real-time PCR，RT-qPCR）法检测心肌组织胰岛素受体底物1（insulin receptor substrate-1，IRS1）、磷酸酰基醇3-激酶（phosphoinosmde-3-kinase，PI3K）、蛋白激酶B（protein kinase，AKT）、GLUT4表达水平；免疫印迹（Western blot，WB）法检测心肌组织PI3K、AKT、GLUT4蛋白表达水平。结果表明，与空白对照组比较，模型组和非经非穴组大鼠一般状态较差，体质量、抓力下降，心肌组织ATP生成量显著降低（P < 0.05），IRS1、PI3K、AKT、GLUT4和GLUT1蛋白表达水平和mRNA水平显著降低（P < 0.05）；与模型组和非经非穴组相比，针刺组大鼠一般状态良好，体质量、抓力上升，心肌组织ATP生成量显著升高（P < 0.05），IRS1、PI3K、AKT、GLUT4和GLUT1蛋白表达水平和mRNA水平显著升高（P < 0.05）。电针“足三里”能改善脾气虚状态下大鼠的心脏能量代谢的机制，可能与激活PI3K/AKT/GLUT4信号通路，提高心肌组织葡萄糖转运功能相关。

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刘继东（1976 -），男（锡伯族），辽宁沈阳人，教授，博士，研究方向：针灸作用机制。

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刘继东（1976 -），男（锡伯族），辽宁沈阳人，教授，博士，研究方向：针灸作用机制。

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