Article(id=1317125442767803223, tenantId=1146029695717560320, journalId=1255847919539208197, issueId=1317125400581485316, articleNumber=null, orderNo=null, doi=10.13193/j.issn.1673-7717.2026.03.020, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=null, receivedDateStr=null, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1791956776562, onlineDateStr=2026-10-14, pubDate=1773072000000, pubDateStr=2026-03-10, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1791956776562, onlineIssueDateStr=2026-10-14, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1791956776562, creator=13701087609, updateTime=1791956776562, updator=13701087609, issue=Issue{id=1317125400581485316, tenantId=1146029695717560320, journalId=1255847919539208197, year='2026', volume='44', issue='3', pageStart='1', pageEnd='258', issueExtLink='null', onlineDate='null', pubDate='1773072000000', pubDateStr='2026-03-10', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1791956766504, creator='13701087609', updateTime=1791958763799, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1317133777898418844, tenantId=1146029695717560320, journalId=1255847919539208197, issueId=1317125400581485316, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1317133777898418845, tenantId=1146029695717560320, journalId=1255847919539208197, issueId=1317125400581485316, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=105, endPage=109, ext={EN=ArticleExt(id=1317125442985907032, articleId=1317125442767803223, tenantId=1146029695717560320, journalId=1255847919539208197, language=EN, title=Effects of Warming Yang and Dredging Vessels Moxibustion on Endothelium-Associated Apoptotic Factors in ApoE
-/- Atherosclerotic Mice via Mitochondrial Pathway, columnId=1256183361521967297, journalTitle=Chinese Archives of Traditional Chinese Medicine, columnName=Target on National Project, runingTitle=null, highlight=null, articleAbstract=
Objective To study the effects of moxibustion method of “warming Yang and dredging vessels” on the morphology and function of mitochondria and the level of apoptosis in ApoE-/- atherosclerosis（AS）mice, and to explore the mechanism of prevention and treatment of AS.
Methods Twenty-two 8-week-old SPF-grade male ApoE-/- mice were randomly allocated into the model group and the moxibustion group, each comprising 11 mice, and the mice were placed on a high-fat diet to establish the atherosclerosis model. Eleven C57BL/6J mice formed the control group and were administered the standard chow. The intervention commenced on the initial day of the modeling for each group, with only the control and model groups being restricted, while the mice in the moxibustion group were positioned at “Danzhong（RN17）”, “Shenque（RN8）” and “Neiguan（PC6）”（bilaterally）. The mice in the moxibustion group received moxibustion at “Danzhong（RN17）”, “Shenque（RN8）”, “Neiguan（PC6）”（bilaterally）and “Xuehai（SP10）”（bilaterally）, administered daily for 30 minutes, five times per week. The samples were collected 12 weeks after intervention. The levels of serum triglyceride（TG）and total cholesterol（TC）were quantified by using an enzymatic assay, while the levels of serum low-density lipoprotein cholesterol（LDL-C）and high-density lipoprotein cholesterol（HDL-C）were assessed by a colorimetric test. The utilization of hematoxylin-eosin（HE）staining facilitated the examination of the pathological morphology and the structure of the thoracic aorta in mice. The transmission electron microscopy was utilized to analyze the morphology of mitochondria in the thoracic aorta, and the whilst flow cytometry（JC-1）was used to evaluate the mitochondrial membrane potential in the thoracic aorta. The changes in mitochondrial membrane potential（MMP）were assessed by using JC-1. The cytochrome C（Cyt C）protein expression in the thoracic aorta was examined through immunofluorescence staining and the protein expression levels of Bax, Bcl-2 and caspase-3 in the thoracic aorta were measured by using immunohistochemistry and Western blot analysis.
Results Compared with those of the control group, the levels of serum TC, TG and LDL-C of mice in the model group were significantly higher（P < 0.01）and HDL-C levels were significantly lower（P < 0.01）. The thoracic aortic structures in mice showed intimal thickening, endothelial cell degeneration, swelling and detachment, and the foam cells were seen. The mitochondria were massively swollen and the number of cristae was greatly reduced. Mitochondrial membrane potential levels were significantly reduced（P < 0.01）. The Cyt C protein levels were significantly higher in thoracic aortic tissue（P < 0.01）. The protein expression levels of Bax and Caspase-3 were significantly higher（P < 0.01）, and the protein expression level of Bcl-2 was significantly lower（P < 0.01）.
Conclusion “Warming Yang and dredging vessels” moxibustion prevents and treats AS by improving the morphology and function of mitochondria and reducing the level of apoptosis.
, authors=Bingxin WU
1, Xiaofang YANG
1, Chaobo YAN
2, Xianming WU
3, 4, Jiaojiao XIONG
1, Zhou ZHU
1, Ning ZHANG
1, Zhihong YANG
1, authorsList=Bingxin WU, Xiaofang YANG, Chaobo YAN, Xianming WU, Jiaojiao XIONG, Zhou ZHU, Ning ZHANG, Zhihong YANG, authorCompany=null, correspAuthors=Zhihong YANG, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1317125443363394393, articleId=1317125442767803223, tenantId=1146029695717560320, journalId=1255847919539208197, language=CN, title=温阳通脉灸法通过线粒体途径对ApoE
-/-动脉粥样硬化小鼠内皮相关凋亡因子的影响, columnId=1256183362172142382, journalTitle=中华中医药学刊, columnName=国家项目点击, runingTitle=null, highlight=null, articleAbstract=
目的 研究“温阳通脉”灸法对ApoE-/-动脉粥样硬化（atherosclerosis，AS）小鼠线粒体形态、功能及细胞凋亡水平的影响，探讨其防治AS的作用机制。
方法 8周龄SPF级雄性ApoE-/-小鼠22只，随机分为模型组和艾灸组，每组11只，高脂饲料饲养复制AS模型。11只C57BL/6J小鼠作为对照组，普通饲料饲养。各组小鼠均在造模第1天开始干预，对照组、模型组仅抓取固定，艾灸组小鼠于“膻中”“神阙”“内关”（双侧）及“血海”（双侧）穴处行艾灸治疗，每日1次，每次30 min，每周连续5次，共干预12周后取材。用酶法测定血清甘油三酯（TG）、总胆固醇（TC）含量，比色法测定血清低密度脂蛋白胆固醇（LDL-C）、高密度脂蛋白胆固醇（HDL-C）含量；苏木精-伊红（HE）染色观察小鼠胸主动脉病理形态结构；透射电镜观察小鼠胸主动脉线粒体形态；流式细胞术（JC-1）检测胸主动脉线粒体膜电位（MMP）变化；免疫荧光染色法检测胸主动脉Cytochrome C（Cyt C）蛋白表达；免疫组化和Western blot法检测胸主动脉Bax、Bcl-2、Caspase-3蛋白表达。
结果 与对照组比较，模型组小鼠血清TC、TG和LDL-C含量显著升高（P < 0.01），HDL-C含量降低（P < 0.01）；小鼠的胸主动脉结构呈血管内膜增厚，内皮细胞变性、肿胀和脱落，可见泡沫细胞；线粒体大量肿胀，嵴数量大大减少；线粒体膜电位水平明显下降（P < 0.01）；胸主动脉组织Cyt C蛋白含量明显升高（P < 0.01）；Bax、Caspase-3蛋白表达水平明显升高（P < 0.01），Bcl-2蛋白表达水平明显降低（P < 0.01）。与模型组比较，艾灸组小鼠血清TC、TG、LDL-C含量显著下降（P < 0.05或P < 0.01）；小鼠胸主动脉管腔较为规则，内膜可见少量的内皮细胞变性和肿胀；线粒体轻度肿胀，嵴结构排列较整齐；线粒体膜电位水平明显上升（P < 0.01）；胸主动脉组织Cyt C蛋白表达含量明显降低（P < 0.01），Bax、Caspase-3蛋白表达水平明显降低（P < 0.01），Bcl-2蛋白表达水平明显升高（P < 0.01）。
结论 “温阳通脉”灸法通过改善线粒体形态及功能，降低细胞凋亡水平，从而防治AS。
, authors=吴冰心
1, 杨孝芳
1, 闫朝勃
2, 伍先明
3, 4, 熊娇娇
1, 朱洲
1, 张宁
1, 杨志虹
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吴冰心（1998 -），女（仡佬族），贵州务川人，硕士在读，研究方向：针灸治疗心脑血管系统疾病临床及机制。
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吴冰心（1998 -），女（仡佬族），贵州务川人，硕士在读，研究方向：针灸治疗心脑血管系统疾病临床及机制。
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吴冰心（1998 -），女（仡佬族），贵州务川人，硕士在读，研究方向：针灸治疗心脑血管系统疾病临床及机制。
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2.长沙市中医院（长沙市第八医院），湖南 长沙 410007, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1319724559939514482, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1317125442767803223, xref=2., ext=[AuthorCompanyExt(id=1319724559952097395, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1317125442767803223, companyId=1319724559939514482, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=
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3.Second Clinical School of Medicine, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, Guizhou, China
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3.贵州中医药大学第二临床医学院，贵州 贵阳 550025
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1, address=
1.School of Acupuncture and Tuina, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, Guizhou, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1319724562795835540, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1317125442767803223, authorId=1319724562619674769, language=CN, stringName=熊娇娇, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=
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北京市海淀区学院南路86号
010-62662699（运营及技术支持）
qkjq@cast.org.cn
