中华中医药学刊

雷公藤多苷通过TFEB-溶酶体途径诱导骨肉瘤细胞铁死亡的调控机制

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官博雅 ¹^,² , 葛振华 ¹^,² , 张金红 ² , 陈青 ² , 徐亮 ¹ , 冯鑫 ²

中华中医药学刊 | 国家项目点击 2026,44(4): 90-94

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中华中医药学刊 |国家项目点击 2026 , 44 (4) : 90 -94

雷公藤多苷通过TFEB-溶酶体途径诱导骨肉瘤细胞铁死亡的调控机制

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官博雅¹^,², 葛振华¹^,², 张金红², 陈青², 徐亮¹, 冯鑫²

作者信息

^1.天津医科大学药学院，天津 300074

^2.天津市天津医院药学部，天津 300211

通讯作者:

徐亮（1982-），男，内蒙古兴安盟乌兰浩特人，教授，博士研究生导师，博士，研究方向：药物分析。E-mail: xuliang@tmu.edu.cn。

冯鑫（1972-），女，天津人，主任药师，硕士，研究方向：临床药学，药物经济学，药事管理学。E-mail: fx_yutong@126.com。

作者简介:

官博雅（1988-），女，天津人，主管药师，硕士，研究方向：抗肿瘤药物的作用机制及疗效评价。

Mechanism of Tripterygium Glycosides-Induced Ferroptosis in Osteosarcoma Cells via TFEB-Lysosome Pathway

Boya GUAN¹^,², Zhenhua GE¹^,², Jinhong ZHANG², Qing CHEN², Liang XU¹, Xin FENG²

Affiliations

^1.School of Pharmacy, Tianjin Medical University, Tianjin 300074, China

^2.Department of Pharmacy, Tianjin Hospital, Tianjin 300211, China

出版时间: 2026-04-10 doi: 10.13193/j.issn.1673-7717.2026.04.020

文章导航

摘要

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目的

研究雷公藤多苷（TG）通过转录因子EB（TFEB）-溶酶体途径对骨肉瘤细胞铁死亡的调控作用。

方法

培养骨肉瘤U2OS细胞，将其分为对照组、不同浓度TG组（10、20、40、80、160、320 μmol/L TG）、对照（NC）组（转染NC siRNA）、NC + TG组（转染NC siRNA联合160 μmol/L TG）、si-TFEB + TG组（转染TFEB siRNA联合160 μmol/L TG）。CCK-8法检测细胞增殖活力，荧光显微镜检测溶酶体探针LysoTracker的荧光强度，酶标仪检测Fe²⁺及活性氧（ROS）水平，Western blot检测总蛋白及核蛋白中TFEB的表达水平以及溶酶体相关蛋白[溶酶体相关膜蛋白1（LAMP1）、溶酶体相关膜蛋白2（LAMP2）、组织蛋白酶B（CTSB）、组织蛋白酶D（CTSD）]、铁死亡相关蛋白[谷胱甘肽过氧化物酶4（GPX4）、溶质载体家族成员SLC7A11（SLC7A11）]的表达水平。

结果

不同浓度TG组的存活率均低于对照组（P<0.05）且雷公藤多苷浓度越高、存活率越低；低、中、高浓度TG（40、80、160 μmol/L）组的LysoTracker荧光强度及总蛋白中LAMP1、LAMP2、GPX4、SLC7A11的表达水平均低于对照组，Fe²⁺、ROS水平及核蛋白中TFEB的表达水平、总蛋白中CTSB及CTSD的表达水平高于对照组（P<0.05）；si-TFEB质粒TG组的存活率、LysoTracker荧光强度及总蛋白中LAMP1、LAMP2、GPX4、SLC7A11的表达水平均高于NC + TG组，Fe²⁺、ROS水平及核蛋白中TFEB的表达水平、总蛋白中CTSB及CTSD的表达水平低于NC + TG组（P<0.05）。

结论

雷公藤多苷通过促进TFEB入核的方式影响溶酶体功能，进而促进骨肉瘤细胞发生铁死亡。

关键词

骨肉瘤 / 雷公藤多苷 / 铁死亡 / 溶酶体 / 转录因子EB

Abstract

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Objective

To study the regulatory effect of tripterygium glycosides（TG）on ferroptosis of osteosarcoma cells via transcription factor EB（TFEB）-lysosome pathway.

Method

Osteosarcoma U2OS cells were cultured and divided into control group, TG groups with different concentrations（10, 20, 40, 80, 160, 320 μmol/L）, negative control（NC）group（transfected with NC siRNA）, and NC + TG group（transfected with NC siRNA combined with 160 μmol/L TG）, si-TFEB + TG（transfected with TFEB siRNA combined with 160 μmol/L TG）. Cell proliferation activity was detected by CCK-8 assay, lysosome probe Lyso-Tracker activity was detected by flow cytometry, Fe²⁺ and reactive oxygen species（ROS）levels were detected by enzymolysis assay. Western blot analysis was performed to detect the expression levels of TFEB in total protein and nuclear protein and the expression levels of lysosome related proteins（LAMP1, LAMP2, CTSB, CTSD）and ferroptosis related proteins（GPX4, SLC7A11）in total protein.

Results

The survival rate of TG groups with different concentrations was lower than that of the control group（P<0.05）, and the higher the concentration of TG, the lower the survival rate. The LysoTracker activity and the expression levels of LAMP1, LAMP2, GPX4 and SLC7A11 in total protein of low, medium and high concentration TG groups（40, 80, 160 μmol/L） were lower than those in the control group, the expression levels of Fe²⁺, ROS and TFEB in nuclear protein, the expressions of CTSB and CTSD in total protein were higher than those in the control group（P<0.05）. The survival rate, LysoTracker activity and the expression levels of LAMP1, LAMP2, GPX4 and SLC7A11 in total protein of si-TFEB + TG group were higher than those in the NC + TG group, the expression levels of Fe²⁺, ROS and TFEB in nuclear protein, the expression of CTSB and CTSD in total protein were lower than those in the NC + TG group（P<0.05）.

Conclusion

TG affects lysosomal function by promating TFEB nuclear translocation, and then promotes ferroptosis in osteosarcoma cells.

Key words

osteosarcoma / tripterygium glycosides / ferroptosis / lysosome / transcription factor EB

引用本文

官博雅, 葛振华, 张金红, 陈青, 徐亮, 冯鑫. 雷公藤多苷通过TFEB-溶酶体途径诱导骨肉瘤细胞铁死亡的调控机制. 中华中医药学刊, 2026 , 44 (4) : 90 -94 . DOI: 10.13193/j.issn.1673-7717.2026.04.020

Boya GUAN, Zhenhua GE, Jinhong ZHANG, Qing CHEN, Liang XU, Xin FENG. Mechanism of Tripterygium Glycosides-Induced Ferroptosis in Osteosarcoma Cells via TFEB-Lysosome Pathway[J]. Chinese Archives of Traditional Chinese Medicine, 2026 , 44 (4) : 90 -94 . DOI: 10.13193/j.issn.1673-7717.2026.04.020

基金

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国家自然科学基金项目(81402889)
天津市卫生健康委员会中医中西医结合科研项目(2023176)

参考文献

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2026年第44卷第4期

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doi: 10.13193/j.issn.1673-7717.2026.04.020

首发时间：2026-10-14
出版时间：2026-04-10

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基金

国家自然科学基金项目(81402889)

天津市卫生健康委员会中医中西医结合科研项目(2023176)

作者信息

^1.天津医科大学药学院，天津 300074

^2.天津市天津医院药学部，天津 300211

通讯作者:

徐亮（1982-），男，内蒙古兴安盟乌兰浩特人，教授，博士研究生导师，博士，研究方向：药物分析。E-mail: xuliang@tmu.edu.cn。

冯鑫（1972-），女，天津人，主任药师，硕士，研究方向：临床药学，药物经济学，药事管理学。E-mail: fx_yutong@126.com。

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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