中华中医药学刊

黄芪多糖对肺癌荷瘤小鼠机体免疫功能、肿瘤细胞生长的调节作用

PDF下载

涂建国 ¹ , 曹文淼 ² , 汪步海 ²

中华中医药学刊 | 国家项目点击 2026,44(4): 17-20

收起

中华中医药学刊 |国家项目点击 2026 , 44 (4) : 17 -20

黄芪多糖对肺癌荷瘤小鼠机体免疫功能、肿瘤细胞生长的调节作用

全屏

[Author(id=1319630617629803069, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1317115123811074187, orderNo=0, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1319630617751437887, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1317115123811074187, authorId=1319630617629803069, language=EN, stringName=Jianguo TU, firstName=Jianguo, middleName=null, lastName=TU, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=¹, address=^1.School of Medicine, Yangzhou University, Yangzhou 225009, Jiangsu, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1319630617831129664, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1317115123811074187, authorId=1319630617629803069, language=CN, stringName=涂建国, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=¹, address=^1.扬州大学医学院，江苏扬州 225009, bio={"content":"

涂建国（1974-），男，江苏宝应人，主任医师，学士，研究方向：肺癌。

"}, bioImg=null, bioContent=

涂建国（1974-），男，江苏宝应人，主任医师，学士，研究方向：肺癌。

, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1319630617399116342, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1317115123811074187, xref=1., ext=[AuthorCompanyExt(id=1319630617411699255, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1317115123811074187, companyId=1319630617399116342, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=^1.School of Medicine, Yangzhou University, Yangzhou 225009, Jiangsu, China), AuthorCompanyExt(id=1319630617424282168, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1317115123811074187, companyId=1319630617399116342, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=^1.扬州大学医学院，江苏扬州 225009)])]), Author(id=1319630617923404354, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1317115123811074187, orderNo=1, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1319630618007290436, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1317115123811074187, authorId=1319630617923404354, language=EN, stringName=Wenmiao CAO, firstName=Wenmiao, middleName=null, lastName=CAO, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=², address=^2.Subei People＇s Hospital Affiliated to Yangzhou University, Yangzhou 225001, Jiangsu, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1319630618095370821, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1317115123811074187, authorId=1319630617923404354, language=CN, stringName=曹文淼, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=², address=^2.扬州大学附属苏北人民医院，江苏扬州 225001, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1319630617524945465, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1317115123811074187, xref=2., ext=[AuthorCompanyExt(id=1319630617537528378, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1317115123811074187, companyId=1319630617524945465, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=^2.Subei People＇s Hospital Affiliated to Yangzhou University, Yangzhou 225001, Jiangsu, China), AuthorCompanyExt(id=1319630617550111291, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1317115123811074187, companyId=1319630617524945465, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=^2.扬州大学附属苏北人民医院，江苏扬州 225001)])]), Author(id=1319630618191839815, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1317115123811074187, orderNo=2, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1319630618300891721, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1317115123811074187, authorId=1319630618191839815, language=EN, stringName=Buhai WANG, firstName=Buhai, middleName=null, lastName=WANG, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=², address=^2.Subei People＇s Hospital Affiliated to Yangzhou University, Yangzhou 225001, Jiangsu, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1319630618384777802, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1317115123811074187, authorId=1319630618191839815, language=CN, stringName=汪步海, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=², address=^2.扬州大学附属苏北人民医院，江苏扬州 225001, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1319630617524945465, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1317115123811074187, xref=2., ext=[AuthorCompanyExt(id=1319630617537528378, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1317115123811074187, companyId=1319630617524945465, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=^2.Subei People＇s Hospital Affiliated to Yangzhou University, Yangzhou 225001, Jiangsu, China), AuthorCompanyExt(id=1319630617550111291, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1317115123811074187, companyId=1319630617524945465, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=^2.扬州大学附属苏北人民医院，江苏扬州 225001)])])]

涂建国¹, 曹文淼², 汪步海²

作者信息

^1.扬州大学医学院，江苏扬州 225009

^2.扬州大学附属苏北人民医院，江苏扬州 225001

作者简介:

涂建国（1974-），男，江苏宝应人，主任医师，学士，研究方向：肺癌。

Regulatory Effect of Astragalus Polysaccharide on Immune Function and Tumor Cell Growth of Lung Cancer Mice

Jianguo TU¹, Wenmiao CAO², Buhai WANG²

Affiliations

^1.School of Medicine, Yangzhou University, Yangzhou 225009, Jiangsu, China

^2.Subei People＇s Hospital Affiliated to Yangzhou University, Yangzhou 225001, Jiangsu, China

出版时间: 2026-04-10 doi: 10.13193/j.issn.1673-7717.2026.04.004

文章导航

摘要

收起

目的

探讨黄芪多糖对肺癌荷瘤小鼠机体免疫、肿瘤细胞生长的调节作用。

方法

选取无特定病原体（specific pathogen free，SPF）级的C57BL/6J小鼠72只，随机分为模型组（等量生理盐水灌胃）、阳性对照组（顺铂6.0 mg/kg腹腔注射）、低剂量组（黄芪多糖溶液50 mg/kg灌胃，浓度为1.0 mg/mL）、高剂量组（黄芪多糖溶液100 mg/kg灌胃，浓度为1.0 mg/mL）各18只，对比各组小鼠造模后第14天肿瘤体积、肿瘤瘤体质量、抑瘤率、外周血中T淋巴细胞、肿瘤组织中Survivin蛋白表达，采用流式细胞技术检测各组小鼠肿瘤组织中肿瘤细胞的分期分布情况。

结果

阳性对照组、低剂量组、高剂量组的肿瘤体积、肿瘤质量与模型组比较均更低（P<0.05）；阳性对照组的肿瘤体积、肿瘤质量与低剂量组、高剂量组小鼠比较明显更低（P<0.05），抑瘤率相较于低剂量组、高剂量组则明显更高（P<0.05）；高剂量组的肿瘤体积、肿瘤质量均低于低剂量组（P<0.05），抑瘤率高于低剂量组（P<0.05）；低剂量组、高剂量组外周血中CD4⁺T淋巴细胞（cluster of differentiation 4，CD4⁺）、CD4⁺/CD8⁺T淋巴细胞（cluster of differentiation 8，CD8⁺）高于模型组和阳性对照组（P<0.05），CD8⁺水平低于模型组和阳性对照组（P<0.05）；高剂量组外周血中CD4⁺、CD4⁺/CD8⁺高于低剂量组（P<0.05），CD8⁺水平低于低剂量组（P<0.05）；阳性对照组、低剂量组、高剂量组的肿瘤组织中Survivin蛋白评分低于模型组（P<0.05），阳性对照组的Survivin蛋白评分低于低剂量组、高剂量组（P<0.05），高剂量组Survivin蛋白评分低于低剂量组（P<0.05）；阳性对照组、低剂量组、高剂量组的肿瘤组织中S期细胞占比高于模型组（P<0.05），阳性对照组的S期细胞占比高于低剂量组、高剂量组（P<0.05），高剂量组S期细胞占比高于低剂量组（P<0.05）。

结论

黄芪多糖通过调节肺癌荷瘤小鼠免疫功能、降低Survivin蛋白表达，达到抑制肿瘤细胞生长的效果。

关键词

黄芪多糖 / 肺癌 / 荷瘤小鼠 / 机体免疫 / 调节作用

Abstract

收起

Objective

To investigate the regulation effect of astragalus polysaccharide on lung cancer-bearing mice in vivo immunity and tumor cell growth.

Methods

A total of 72 SPF-grade C57BL/6J mice were randomly divided into a model group（treated with an equal volume of normal saline by gavage）, a positive control group（treated with cisplatin 6.0 mg/kg via intraperitoneal injection）, a low-dose group（treated with astragalus polysaccharide solution 50 mg/kg by gavage, at a concentration of 1.0 mg/mL）and a high-dose group（treated with astragalus polysaccharide solution 100 mg/kg by gavage, at a concentration of 1.0 mg/mL）, with 18 mice in each group. The tumor volume, tumor mass, tumor inhibition rate, T lymphocyte count in peripheral blood and Survivin protein expression in tumor tissues were compared among the groups 14 days after modeling. Flow cytometry was used to detect the staging distribution of tumor cells in the tumor tissues of mice in each group.

Results

Compared with those in the model group, the tumor volume and tumor mass in the positive control, low-dose and high-dose groups were all significantly lower（P<0.05）. Specifically, the tumor volume and tumor mass in the positive control group were significantly lower than those in the low-dose and high-dose groups（P<0.05）, while the tumor inhibition rate was significantly higher（P<0.05）. Furthermore, the tumor volume and tumor mass in the high-dose group were lower than those in the low-dose group（P<0.05）, and the tumor inhibition rate was higher（P<0.05）. In terms of peripheral blood, the levels of CD4⁺ and CD4⁺/CD8⁺ in the low-dose and high-dose groups were higher than those in the model and positive control groups（P<0.05）, while the CD8⁺ level was lower（P<0.05）. Additionally, the levels of CD4⁺ and CD4⁺/CD8⁺ in the high-dose group were higher than those in the low-dose group（P<0.05）, and the CD8⁺ level was lower（P<0.05）. Regarding Survivin protein expression in tumor tissues, the scores in the positive control, low-dose and high-dose groups were all lower than those in the model group（P<0.05）. Notably, the Survivin protein expression in the positive control group was lower than that in the low-dose and high-dose groups（P<0.05）, and the expression in the high-dose group was lower than that in the low-dose group（P<0.05）. Finally, the proportions of S-phase cells in tumor tissues were higher in the positive control, low-dose and high-dose groups compared to those in the model group（P<0.05）. Specifically, the proportion of S-phase cells in the positive control group was higher than that in the low-dose and high-dose groups（P<0.05）, and the proportion in the high-dose group was higher than that in the low-dose group（P<0.05）.

Conclusion

Astragalus polysaccharide can regulate the immune function and reduce the expression of Survivin protein in the treatment of lung cancer-bearing mice, thereby inhibiting the growth of tumor cells.

Key words

astragalus polysaccharide / lung cancer / tumor-bearing mice / immunity / regulation

引用本文

涂建国, 曹文淼, 汪步海. 黄芪多糖对肺癌荷瘤小鼠机体免疫功能、肿瘤细胞生长的调节作用. 中华中医药学刊, 2026 , 44 (4) : 17 -20 . DOI: 10.13193/j.issn.1673-7717.2026.04.004

Jianguo TU, Wenmiao CAO, Buhai WANG. Regulatory Effect of Astragalus Polysaccharide on Immune Function and Tumor Cell Growth of Lung Cancer Mice[J]. Chinese Archives of Traditional Chinese Medicine, 2026 , 44 (4) : 17 -20 . DOI: 10.13193/j.issn.1673-7717.2026.04.004

基金

收起

国家中医药管理局项目(20182019)
扬州市重点研发项目(YZ2019057)

参考文献

收起

文献

收起

参考文献引证文献

排序方式：

[1]

庄梦婕，刘丹，陈彦文，等.黄芪多糖联合顺铂抑制小鼠Lewis肺癌移植瘤生长及其机制[J].细胞与分子免疫学杂志，2017，33（4）：503-507.

[2]

ZHUANG MJ, LIU D, CHEN Y W, et al. Inhibitory effect and the mechanism of Astragalus polysaccharide combined with cisplatin on growth of inplanted Lewis lung carcinoma in mice[J]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi, 2017, 33（4）: 503-507.

[3]

庄梦婕，刘丹，陈彦文，等.黄芪多糖联合顺铂对小鼠Lewis肺癌移植瘤Caspase-3、Smac/Diablo表达的影响[J].解剖学报，2018，49（1）：63-69.

[4]

WU C Y, KE Y, ZENG Y F, et al. Anticancer activity of Astragalus polysaccharide in human non-small cell lung cancer cells[J]. Cancer Cell Int, 2017, 17: 115.

[5]

程轩，孟璠璐，孙海凤，等.合并肺癌的多原发恶性肿瘤70例临床病理特征分析[J].现代肿瘤医学，2022，30（23）：4365-4369.

[6]

江雨琛，俞浩，罗鹏飞，等.1990—2019年江苏省肺癌疾病负担及其危险因素变化趋势研究[J].中国肿瘤，2022，31（5）：327-334.

[7]

汪蓓，毛艳，杨淼.黄芪多糖对阿霉素治疗小鼠乳腺癌的增效减毒机制研究[J].中国医院药学杂志，2017，37（6）：497-501.

[8]

张欣，贾英杰，杨佩颖.消岩汤对肺腺癌A549实体荷瘤小鼠肿瘤细胞凋亡干预机制的研究[J].中草药，2017，48（11）：2261-2265.

[9]

陈曦，施京红，吴守振.黄芪多糖在支气管哮喘中的免疫调节作用和相关机制的研究进展[J].中国医药导报，2023，20（31）：56-59.

[10]

刘丹，李杨，王雪林，等.黄芪多糖（APS）及联合顺铂（DDP）抑制小鼠Lewis肺癌复发瘤生长的机制[J].细胞与分子免疫学杂志，2018，34（12）：1105-1110.

[11]

张莹，贾英杰，李小江，等.注射用黄芪多糖联合CIK细胞治疗中晚期气虚型非小细胞肺癌的临床观察[J].中草药，2018，49（7）：1647-1651.

[12]

HONG J N, LI WW, WANG L L, et al. Jiangtang decoction ameliorate diabetic nephropathy through the regulation of PI3K/Aktmediated NF-κB pathways in KK-Ay mice [J]. Chin Med, 2017, 12: 13.

[13]

朱鸿宇，陈鹏，董国璋，等.肺腺癌中免疫微环境特征与EGFR突变状态的相关性研究[J].中国肺癌杂志，2023，26（3）：204-216.

[14]

CUI Y Z, WANG Q J, SUN R, et al. Astragalus membranaceus（Fisch.）Bunge repairs intestinal mucosal injury induced by LPS in mice[J]. BMC Complement Altern Med, 2018, 18（1）: 230.

[15]

周双，朱正秋，倪子龙.外周血淋巴细胞亚群及肿瘤标志物变化对晚期非小细胞肺癌免疫治疗疗效的预测价值[J].医学研究杂志，2023，52（7）：165-170.

[16]

任莉莉，汪文娟，姑丽娜扎尔·艾比布拉，等.支气管哮喘不同炎症表型患者的T淋巴细胞亚群及相关细胞因子的表达水平及意义[J].实用临床医药杂志，2022，26（22）：33-37.

[17]

刘云，卢和丽，邹卿，等.新发HIV/AIDS患者抑郁心理干预效果及其与CD4^T₊♂细胞计数的关系研究[J].中国全科医学，2024，27（32）：4009-4014.

[18]

LU J, SONG H P, TUO Q H, et al. Astragalus polysaccharide relieves reproductive toxicity in phenobarbital-treated epileptic rats[J]. Trop J Pharm Res, 2018, 17（5）: 809.

[19]

苏丽，周敦荣，谭茜茜，等.基于“肺-肠”轴理论分析微生物调节剂对新型冠状病毒肺炎患者肺部菌群、肠道菌群及免疫功能的影响[J].广西医学，2022，44（20）：2407-2412.

[20]

ZHANG M, LIU Y, LI J W, et al. A polysaccharide component from Strongylocentrotus nudus eggs inhibited hepatocellular carcinoma in mice by activating T lymphocytes[J]. Oncol Lett, 2017, 13（3）: 1847-1855.

[21]

张卫平，冉冉，高聚伟，等.黄芪多糖对肺癌外周循环MDSC的疗效影响[J].2017（1）：97-100.

[22]

程艳婷，朱涛，马艳竹，等.麦粒灸通过调控凋亡因子半胱氨酸家族增强环磷酰胺对Hepa1-6肝癌荷瘤小鼠的抗肿瘤作用[J].针刺研究，2023，48（9）：914-922.

[23]

LIU A J, YU J, JI H Y, et al. Extraction of a novel cold-water-soluble polysaccharide from Astragalus membranaceus and its antitumor and immunological activities [J]. Molecules, 2017, 23（1）: 62.

[24]

曹茵，郑锐年，黄珂铭，等.circRNA PRMT5对乳腺癌MCF-7细胞增殖、凋亡和侵袭的影响[J].临床误诊误治，2023，36（4）：142-148.

[25]

ZHAO B J, ZHANG X F, HAN W, et al. Wound healing effect of an Astragalus membranaceus polysaccharide and its mechanism[J]. Mol Med Rep, 2017, 15（6）: 4077-4083.

[26]

张明明，高伟，崔佳，等.黄芪多糖联合洛铂对胃癌细胞的作用效果及分子机制[J].医学动物防制，2019，35（12）：1150-1154.

2026年第44卷第4期

PDF下载

引用本文

BibTeX

文章信息

doi: 10.13193/j.issn.1673-7717.2026.04.004

首发时间：2026-10-14
出版时间：2026-04-10

补充材料

相关文章

文章信息

作者

出版历史

基金

国家中医药管理局项目(20182019)

扬州市重点研发项目(YZ2019057)

作者信息

^1.扬州大学医学院，江苏扬州 225009

^2.扬州大学附属苏北人民医院，江苏扬州 225001

参考文献

分享链接

https://castjournals.cast.org.cn/joweb/zhzyyxk/CN/10.13193/j.issn.1673-7717.2026.04.004

分享至

全文二维码

扫描看全文

引用本文

BibTeX

本文的引用情况

2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

关闭全屏

BibTeX
EndNote
RefWorks
TxT