中华中医药学刊

毛蕊花糖苷对帕金森病小鼠多巴胺细胞保护作用的蛋白组学研究

PDF下载

夏欢 ¹ , 杨珊 ¹ , 朱筱潇 ² , 王子豪 ¹ , 李沛珊 ¹ , 蔡倩 ³ , 杨新玲 ⁴

中华中医药学刊 | 国家项目点击 2026,44(6): 126-130

收起

中华中医药学刊 |国家项目点击 2026 , 44 (6) : 126 -130

毛蕊花糖苷对帕金森病小鼠多巴胺细胞保护作用的蛋白组学研究

全屏

夏欢¹, 杨珊¹, 朱筱潇², 王子豪¹, 李沛珊¹, 蔡倩³, 杨新玲⁴

作者信息

^1.新疆医科大学第二附属医院，新疆乌鲁木齐 830063

^2.陕西中医药大学，陕西咸阳 712046

^3.新疆医科大学附属肿瘤医院，新疆乌鲁木齐 830011

^4.新疆医科大学，新疆乌鲁木齐 830017

通讯作者:

杨新玲（1967-），女，新疆乌鲁木齐人，主任医师、教授，博士研究生导师，研究方向：帕金森病发病机制。E-mail：poplar862@163.com。

作者简介:

夏欢（1987-），男，湖北咸宁人，主治医师，博士在读，研究方向：帕金森病的诊断与治疗。

Proteomics Study on Protection of Dopamine Cell in Mice with Parkinson’s Disease

Huan XIA¹, Shan YANG¹, Xiaoxiao ZHU², Zihao WANG¹, Peishan LI¹, Qian CAI³, Xinling YANG⁴

Affiliations

^1.The Second Affiliated Hospital of Xinjiang Medical University, Urumqi 830063, Xinjiang, China

^2.Shaanxi University of Traditional Chinese Medicine, Xianyang 712046, Shaanxi, China

^3.Tumor Hospital Affiliated to Xinjiang Medical University, Urumqi 830011, Xinjiang, China

^4.Xinjiang Medical University, Urumqi 830017, Xinjiang, China

出版时间: 2026-06-10 doi: 10.13193/j.issn.1673-7717.2026.06.022

文章导航

摘要

收起

目的

探索毛蕊花糖苷（Verbascoside，VB）对帕金森病（Parkinson's disease，PD）小鼠多巴胺细胞保护作用的机制。

方法

纳入63只C57/BL雄性小鼠为实验对象，随机分为空白对照组、MPTP组、10 mg/kg组、30 mg/kg组、60 mg/kg组、120 mg/kg组、180 mg/kg组，每组9只。造模成功后采用矿场、爬杆、悬挂实验评估小鼠行为学改变。采用苏木精-伊红（Hematoxylin-Eosin，HE）、尼氏染色评估小鼠黑质区神经元计数。采用免疫组化和免疫荧光染色分别评估黑质区TH阳性、阳性细胞计数。采用Western blotting检测小鼠黑质区酪氨酸羟化酶（Tyrosine hydroxylase，TH）蛋白的表达情况。采用HE染色评估肝脏和肾脏细胞形态和细胞计数。最后筛选的最佳剂量组黑质组织进行蛋白组学分析。

结果

（1）矿场实验中，120 mg/kg组总路程和中央区的路程均高于MPTP组和其他各浓度梯度组（均P<0.05）；爬杆实验中，120 mg/kg组爬杆时间均低于MPTP组和其他各浓度梯度组（均P<0.05）；悬挂实验中，120 mg/kg组悬挂评分均高于MPTP组和10 mg/kg组（均P<0.05）。（2）HE染色、尼氏染色和免疫组化中，120 mg/kg组细胞计数、棕褐色像素占比均高于MPTP组和其他各浓度梯度组（均P<0.05）。（3）免疫荧光中，120 mg/kg组平均荧光强度均高于MPTP组和其他各浓度梯度组（均P<0.05）。（5）Western blotting中，TH在120 mg/kg组小鼠中的相对表达量均高于MPTP组和其他各浓度梯度组（均P<0.05）。（6）蛋白组学分析显示120 mg/kg组和MPTP组比较，具有统计学差异的蛋白质合计181个，其中上调蛋白83个，下调蛋白98个；亚细胞定位于细胞核的差异蛋白最多有85个，且基因本体（Gene Ontology，GO）功能分析显示最多有6个与免疫直接相关的生物过程（Biological Process，BP）；KEGG通路分析显示与精氨酸生物合成的富集度水平最高（均P<0.05）。

结论

120 mg/kg的VB对PD小鼠的DA神经元具有保护作用，且该保护作用可能与包括精氨酸生物合成在内的多种生物过程有关。

关键词

帕金森病 / 毛蕊花糖苷 / 酪氨酸羟化酶 / 保护作用

Abstract

收起

Objective

To explore the mechanism of verbascoside (VB) on dopamine cell protection in the mice with Parkinson's disease (PD).

Methods

A total of 63 C57/BL male mice were enrolled as the experimental subjects and randomly divided into blank control group, 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP) group, 10 mg/kg group, 30 mg/kg group, 60 mg/kg group, 120 mg/kg group and 180 mg/kg group, with 9 mice in each group. After successful modeling, the behavioral changes of the mice were evaluated by mining, climbing pole and hanging experiments. Hematoxylin-eosin (HE) and Nissl staining were used to evaluate the neuron counts in the substantia nigra of the mice. Immunohistochemistry method and immunofluorescence staining were used to evaluate the tyrosine hydroxylase (TH) -positive and positive cell counts in the substantia nigra, respectively. Western blotting was used to detect the expression of TH protein in the substantia nigra of the mice. HE staining was used to evaluate liver and kidney cell morphology and cell count. Finally, the optimal dose group screened for proteomic analysis was performed.

Results

(1) In the mine experiment, the total distance and the distance of the 120 mg/kg group were higher than those of the MPTP group and other concentration gradient groups (all P<0.05). In the pole climbing experiment, the pole climbing time of the 120 mg/kg group was both higher than that of the MPTP group and other concentration gradient groups (all P<0.05). In the pole climbing experiment, the pole climbing time of the 120 mg/kg group was both higher than that of the pole climbing experiment. The suspension scores of the MPTP group and the other concentration gradient groups (all P<0.05). In the suspension experiment, the suspension scores of the 120 mg/kg group were higher than those of the MPTP group and the 10 mg/kg group (all P<0.05). (2) In HE staining, Niss-type staining and immunohistochemistry method, the cell count and tan pixel proportion of the 120 mg/kg group were higher than those of the MPTP group and other concentration gradient groups (all P<0.05). (3) In immunofluorescence, the average fluorescence intensity of the 120 mg/kg group was higher than that of the MPTP group and other concentration gradient groups (all P<0.05). (5) In Western blotting, the relative expression of TH in the 120 mg/kg group was higher than that in the MPTP group and other concentration gradient groups (all P<0.05). (6) Proteomic analysis showed that compared with the 120 mg/kg group and the MPTP group, there were 181 proteins with statistical differences, including 83 up-regulated proteins and 98 down-regulated proteins. There were up to 85 differential proteins localized to the nucleus of subcellular nucleus, and gene ontology (GO) functional analysis showed that there were up to 6 biological processes directly related to immunity (BP). KEGG pathway analysis showed the highest level of enrichment with arginine biosynthesis (all P<0.05).

Conclusion

VB with the dose of 120 mg/kg has a protective effect on DA neurons in PD mice, and this protective effect may be related to a variety of biological processes including arginine biosynthesis.

Key words

Parkinson's disease / verbascoside / tyrosine hydroxylase / protective function

引用本文

夏欢, 杨珊, 朱筱潇, 王子豪, 李沛珊, 蔡倩, 杨新玲. 毛蕊花糖苷对帕金森病小鼠多巴胺细胞保护作用的蛋白组学研究. 中华中医药学刊, 2026 , 44 (6) : 126 -130 . DOI: 10.13193/j.issn.1673-7717.2026.06.022

Huan XIA, Shan YANG, Xiaoxiao ZHU, Zihao WANG, Peishan LI, Qian CAI, Xinling YANG. Proteomics Study on Protection of Dopamine Cell in Mice with Parkinson’s Disease[J]. Chinese Archives of Traditional Chinese Medicine, 2026 , 44 (6) : 126 -130 . DOI: 10.13193/j.issn.1673-7717.2026.06.022

基金

收起

国家自然科学基金项目(82371258)
新疆维吾尔自治区重点研发任务专项(2023B03003)
新疆维吾尔自治区天山英才-科技创新领军人才项目(2022TSYCLJ0066)
新疆维吾尔自治区研究生科研创新项目(XJ2024G160)

参考文献

收起

文献

收起

参考文献引证文献

排序方式：

［1］

刘康慧，张淑岩．坏死性凋亡在帕金森病病理机制中的研究进展［J］．中华神经医学杂志，2024，23（7）：729-734.

［2］

金刚，李英真，施维，等．帕金森病在病理生理学中的研究进展［J/OL］．中华脑科疾病与康复杂志（电子版），2023，13（5）：315-319.

［3］

DE PAIVA LOPES K，SNIJDERS G J L，HUMPHREY J，et al. Genetic analysis of the human microglial transcriptome across brain regions，aging and disease pathologies［J］. Nat Genet，2022，54（1）：4-17.

［4］

LI M Q，ZHOU F，XU T，et al. Acteoside protects against 6-OHDA-induced dopaminergic neuron damage via Nrf2-ARE signaling pathway［J］. Food Chem Toxicol，2018，119：6-13.

［5］

ZHOU H W，ZHANG C，HUANG C R. Verbascoside attenuates acute inflammatory injury caused by an intracerebral hemorrhage through the suppression of NLRP3［J］. Neurochem Res，2021，46（4）：770-777.

［6］

GAO L，PENG X M，HUO S X，et al. Memory enhancement of acteoside（verbascoside）in a senescent mice model induced by a combination of D-gal and AlCl₃［J］. Phytother Res，2015，29（8）：1131-1136.

［7］

PENG X M，GAO L，HUO S X，et al. The mechanism of memory enhancement of acteoside（verbascoside）in the senescent mouse model induced by a combination of D-gal and AlCl₃［J］. Phytother Res，2015，29（8）：1137-1144.

［8］

张之菁，梁建庆．中药有效成分治疗帕金森病的实验研究进展［J］．辽宁中医杂志，2024，51（9）：216-220.

［9］

CHAO C L，HUANG H W，HUANG H C，et al. Inhibition of amyloid beta aggregation and deposition of cistanche tubulosa aqueous extract［J］. Molecules，2019，24（4）：687.

［10］

CUI Q L，PAN Y N，BAI X W，et al. Systematic characterization of the metabolites of echinacoside and acteoside from Cistanche tubulosa in rat plasma，bile，urine and feces based on UPLC-ESI-Q-TOF-MS［J］. Biomed Chromatogr，2016，30（9）：1406-1415.

［11］

QIN C，HU S Q，ZHANG S X，et al. Hydroxytyrosol acetate improves the cognitive function of APP/PS1 transgenic mice in ERβ-dependent manner［J］. Mol Nutr Food Res，2021，65（3）：e2000797.

［12］

GUO Z L，QIAN Q Y，XIAOLIN L I，et al. Efficacy of verbascoside，echinacoside，crenatoside on altitude-induced fatigue in rats and possible mechanism［J］. J Tradit Chin Med，2023，43（5）：934-943.

［13］

ROSSI R，MAINARDI E，VIZZARRI F，et al. Verbascoside-rich plant extracts in animal nutrition［J］. Antioxidants，2023，13（1）：39.

［14］

PONGKITWITOON B，PUTALUN W，TRIWITAYAKORN K，et al. Anti-inflammatory activity of verbascoside- and isoverbascoside-rich Lamiales medicinal plants［J］. Heliyon，2023，10（1）：e23644.

［15］

DANESHFOROUZ A，NAZEMI S，GHOLAMI O，et al. The cytotoxicity and apoptotic effects of verbascoside on breast cancer 4T1 cell line［J］. BMC Pharmacol Toxicol，2021，22（1）：72.

［16］

ZHU X F，SUN M，GUO H M，et al. Verbascoside protects from LPS-induced septic cardiomyopathy via alleviating cardiac inflammation，oxidative stress and regulating mitochondrial dynamics［J］. Ecotoxicol Environ Saf，2022，233：113327.

［17］

GOMES D B，SERPA P Z，MIORANDO D，et al. Involvement of anti-inflammatory and stress oxidative markers in the antidepressant-like activity of aloysia citriodora and verbascoside on mice with bacterial lipopolysaccharide-（LPS-）induced depression［J］. Evid Based Complement Alternat Med，2022，2022：1041656.

［18］

SMEJKAL K，BABULA P，SLAPETOVÁ T，et al. Cytotoxic activity of C-geranyl compounds from Paulownia tomentosa fruits［J］. Planta Med，2008，74（12）：1488-1491.

［19］

BUDZIANOWSKA A，TOTOŃ E，ROMANIUK-DRAPAŁA A，et al. Cytotoxic effect of phenylethanoid glycosides isolated from plantago lanceolata L［J］. Life，2023，13（2）：556.

［20］

ŞENOL H，TULAY P，ERGÖREN M Ç，et al. Cytotoxic effects of verbascoside on MCF-7 and MDA-MB-231［J］. Turk J Pharm Sci，2021，18（5）：637-644.

［21］

HOSSEINZAD H，ETEMAD L，ZAFARI R，et al. Acute，sub-acute and cell toxicity of verbascoside［J］. Res J Med Plant，2015，9（7）：354-360.

［22］

GUTIÉRREZ-REBOLLEDO G A，GARDUÑO-SICILIANO L，GARCÍA-RODRÍGUEZ R V，et al. Anti-inflammatory and toxicological evaluation of Moussonia deppeana（Schldl. & Cham）Hanst and Verbascoside as a main active metabolite［J］. J Ethnopharmacol，2016，187：269-280.

［23］

CHEN S S，LIU H H，WANG S M，et al. The neuroprotection of verbascoside in Alzheimer’s disease mediated through mitigation of neuroinflammation via blocking NF-κB-p65 signaling［J］. Nutrients，2022，14（7）：1417.

［24］

谢良骐，柴智，弓强，等．毛蕊花糖苷对中枢神经系统的作用及其机制研究进展［J］．中华中医药学刊，2024，42（7）：179-182.

［25］

QU Y J，DING M R，GU C，et al. Acteoside and ursolic acid synergistically protects H₂O₂-induced neurotrosis by regulation of AKT/mTOR signalling：from network pharmacology to experimental validation［J］. Pharm Biol，2022，60（1）：1751-1761.

［26］

AIMAITI M，WUMAIER A，AISA Y，et al. Acteoside exerts neuroprotection effects in the model of Parkinson’s disease via inducing autophagy：Network pharmacology and experimental study［J］. Eur J Pharmacol，2021，903：174136.

［27］

黄晨蓉，张强，曾芳月．益肾除颤汤改善帕金森病患者认知功能的研究［J］．辽宁中医杂志，2025，52（2）：89-93.

2026年第44卷第6期

PDF下载

引用本文

BibTeX

文章信息

doi: 10.13193/j.issn.1673-7717.2026.06.022

首发时间：2026-10-14
出版时间：2026-06-10

补充材料

相关文章

文章信息

作者

出版历史

基金

国家自然科学基金项目(82371258)

新疆维吾尔自治区重点研发任务专项(2023B03003)

新疆维吾尔自治区天山英才-科技创新领军人才项目(2022TSYCLJ0066)

新疆维吾尔自治区研究生科研创新项目(XJ2024G160)

作者信息

^1.新疆医科大学第二附属医院，新疆乌鲁木齐 830063

^2.陕西中医药大学，陕西咸阳 712046

^3.新疆医科大学附属肿瘤医院，新疆乌鲁木齐 830011

^4.新疆医科大学，新疆乌鲁木齐 830017

通讯作者:

杨新玲（1967-），女，新疆乌鲁木齐人，主任医师、教授，博士研究生导师，研究方向：帕金森病发病机制。E-mail：poplar862@163.com。

参考文献

分享链接

https://castjournals.cast.org.cn/joweb/zhzyyxk/CN/10.13193/j.issn.1673-7717.2026.06.022

分享至

全文二维码

扫描看全文

引用本文

BibTeX

本文的引用情况

2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

关闭全屏

BibTeX
EndNote
RefWorks
TxT