中华中医药学刊

基于转录组测序探讨眼针治疗急性缺血性脑卒中的机制

PDF下载

杨士成 , 海英

中华中医药学刊 | 国家项目点击 2026,44(9): 129-132

收起

中华中医药学刊 |国家项目点击 2026 , 44 (9) : 129 -132

基于转录组测序探讨眼针治疗急性缺血性脑卒中的机制

全屏

[Author(id=1319291251455799375, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1319291247567679560, orderNo=0, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1319291251543879761, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1319291247567679560, authorId=1319291251455799375, language=EN, stringName=Shicheng YANG, firstName=Shicheng, middleName=null, lastName=YANG, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=Department of Neurology, Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang 110032, Liaoning, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1319291251619377234, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1319291247567679560, authorId=1319291251455799375, language=CN, stringName=杨士成, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=辽宁中医药大学附属医院，辽宁　沈阳　110032, bio={"content":"

杨士成（1993-），男，辽宁沈阳人，主治医师，博士在读，研究方向：针灸治疗神经系统疾病。

"}, bioImg=null, bioContent=

杨士成（1993-），男，辽宁沈阳人，主治医师，博士在读，研究方向：针灸治疗神经系统疾病。

, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1319291251371913291, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1319291247567679560, xref=null, ext=[AuthorCompanyExt(id=1319291251380301900, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1319291247567679560, companyId=1319291251371913291, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=Department of Neurology, Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang 110032, Liaoning, China), AuthorCompanyExt(id=1319291251384496205, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1319291247567679560, companyId=1319291251371913291, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=辽宁中医药大学附属医院，辽宁　沈阳　110032)])]), Author(id=1319291251711651924, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1319291247567679560, orderNo=1, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=1, authorType=1, ext={EN=AuthorExt(id=1319291251791343702, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1319291247567679560, authorId=1319291251711651924, language=EN, stringName=Ying HAI, firstName=Ying, middleName=null, lastName=HAI, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=Department of Neurology, Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang 110032, Liaoning, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1319291251862646871, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1319291247567679560, authorId=1319291251711651924, language=CN, stringName=海英, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=辽宁中医药大学附属医院，辽宁　沈阳　110032, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1319291251371913291, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1319291247567679560, xref=null, ext=[AuthorCompanyExt(id=1319291251380301900, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1319291247567679560, companyId=1319291251371913291, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=Department of Neurology, Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang 110032, Liaoning, China), AuthorCompanyExt(id=1319291251384496205, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1319291247567679560, companyId=1319291251371913291, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=辽宁中医药大学附属医院，辽宁　沈阳　110032)])])]

杨士成, 海英

作者信息

辽宁中医药大学附属医院，辽宁　沈阳　110032

通讯作者:

海英（1969-），女（蒙古族），辽宁阜新人，教授、主任医师，博士研究生导师，博士，研究方向：针灸治疗神经系统疾病。

作者简介:

杨士成（1993-），男，辽宁沈阳人，主治医师，博士在读，研究方向：针灸治疗神经系统疾病。

Exploring Mechanism of Eye Acupuncture in Treating Acute Ischemic Stroke Based on Transcriptome Sequencing

Shicheng YANG, Ying HAI

Affiliations

Department of Neurology, Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang 110032, Liaoning, China

出版时间: 2026-09-10 doi: 10.13193/j.issn.1673-7717.2026.09.024

文章导航

摘要

收起

目的

基于转录组测序探讨眼针治疗急性缺血性脑卒中（AIS）的机制。

方法

纳入32例符合纳排标准的急性缺血性脑卒中患者，分为对照组及眼针组，观察干预前后的神经功能评分，收集患者治疗后的外周静脉血并进行转录组测序，比较得到差异表达基因（DEGs）并进行基因本体论（GO）功能注释和京都基因与基因组百科全书（KEGG）通路富集分析以及蛋白质互作用（PPI）网络分析。

结果

在规范化内科治疗的基础上给予眼针治疗可显著改善患者神经功能缺损症状。转录组测序结果对比发现差异表达基因501个，其中在眼针组中上调差异基因175个，下调差异基因326个。GO分析发现差异表达基因的功能主要富集在生产分子介质以介导免疫反应、细胞对白细胞介素-1的响应、发热的产生、对趋化因子的响应、细胞对趋化因子的响应等方面。KEGG分析表明差异表达基因主要富集在涉及炎症免疫及细胞凋亡的传导途径中。PPI分析揭示了肿瘤坏死因子（TNF）、白细胞介素1A（IL1A）、趋化因子2（CCL2）可作为关键基因。

结论

对炎症免疫反应及细胞凋亡的调控可能是眼针治疗急性缺血性脑卒中较为关键的机制。

关键词

眼针 / 缺血性脑卒中 / 转录组测序 / 炎症免疫反应 / 细胞凋亡

Abstract

收起

Objective

To investigate the mechanism of eye acupuncture in acute ischemic stroke based on transcriptome sequencing.

Methods

Thirty-two patients with acute ischemic stroke who met the inclusion and exclusion criteria were enrolled and divided into a control group and an eye acupuncture group. The neurological function scores before and after the intervention were observed. Peripheral venous blood of the patients after treatment was collected and subjected to transcriptome sequencing. Differentially expressed genes (DEGs) were obtained through comparison, and Gene Ontology (GO) functional annotation, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis and protein-protein interaction (PPI) network analysis were carried out.

Results

Based on standardized internal medical treatment, the administration of eye acupuncture treatment could significantly improve the neurological deficit symptoms of the patients. Comparison of the transcriptome sequencing results revealed 501 differentially expressed genes, among which 175 genes were upregulated and 326 genes were downregulated in the eye acupuncture group. GO analysis showed that the functions of the differentially expressed genes were mainly enriched in aspects such as the production of molecular mediators to mediate the immune response, the cellular response to interleukin-1, the generation of fever, the response to chemokines and the cellular response to chemokines. KEGG analysis indicated that the differentially expressed genes were mainly enriched in the signaling pathways involving inflammation, immunity and apoptosis. PPI analysis revealed that tumor necrosis factor (TNF), interleukin 1A (IL1A) and chemokine 2 (CCL2) could serve as key genes.

Conclusion

The regulation of the inflammatory immune response and apoptosis may be a crucial mechanism for the treatment of acute ischemic stroke by eye acupuncture.

Key words

eye acupuncture / acute ischemic stroke / transcriptome sequencing / inflammatory immune responses / apoptosis

引用本文

杨士成, 海英. 基于转录组测序探讨眼针治疗急性缺血性脑卒中的机制. 中华中医药学刊, 2026 , 44 (9) : 129 -132 . DOI: 10.13193/j.issn.1673-7717.2026.09.024

Shicheng YANG, Ying HAI. Exploring Mechanism of Eye Acupuncture in Treating Acute Ischemic Stroke Based on Transcriptome Sequencing[J]. Chinese Archives of Traditional Chinese Medicine, 2026 , 44 (9) : 129 -132 . DOI: 10.13193/j.issn.1673-7717.2026.09.024

基金

收起

国家中医药管理局田维柱全国名中医传承工作室项目(国中医药办人教函〔2022〕245号)
海英辽宁省名中医传承工作室(辽卫发〔2021〕7号)
辽宁省“兴辽英才计划”项目(XLYC2002104)

参考文献

收起

文献

收起

参考文献引证文献

排序方式：

[1]

WALTER K. What is acute ischemic stroke?[J]. Jama, 2022, 327(9): 885.

[2]

ZHU Y H, SUN Y L, HU J C, et al. Insight into the mechanism of exercise preconditioning in ischemic stroke[J]. Front Pharmacol, 2022, 13: 866360.

[3]

CAMPBELL B C V, KHATRI P. Stroke[J]. Lancet, 2020, 396(10244): 129-142.

[4]

车戬, 海英. 眼针实践录—田维柱临证经验集[M]. 北京: 人民卫生出版社, 2013: 15-16.

[5]

LOCKHART D J, WINZELER E A. Genomics, gene expression and DNA arrays[J]. Nature, 2000, 405(6788): 827-836.

[6]

HRDLICKOVA R, TOLOUE M, TIAN B. RNA-Seq methods for transcriptome analysis[J]. Wiley Interdiscip Rev RNA, 2017, 8(1): 102-105.

[7]

SEGUNDO-VAL I S, SANZ-LOZANO C S. Introduction to the gene expression analysis[J]. Methods Mol Biol, 2016, 1434: 29-43.

[8]

李艳荣, 李瑞雪, 樊慧杰, 等. 转录组学技术在中医药领域的应用研究进展[J]. 时珍国医国药, 2022, 33(4): 943-947.

[9]

霍宏, 董旭, 崔一之, 等. 转录组学应用于针灸作用机制研究的探讨[J]. 针灸临床杂志, 2022, 38(1): 1-7.

[10]

QIN S R, ZHANG Z C, ZHAO Y D, et al. The impact of acupuncture on neuroplasticity after ischemic stroke: a literature review and perspectives[J]. Front Cell Neurosci, 2022, 16: 817732.

[11]

WANG L D, CHI X S, LYU J, et al. An overview of the evidence to guide decision-making in acupuncture therapies for early recovery after acute ischemic stroke[J]. Front Neurol, 2022, 13: 1005819.

[12]

曹久冬, 吕学玉, 霍然. 眼针治疗脑卒中及其并发症研究进展[J]. 陕西中医, 2021, 42(12): 1819-1821.

[13]

钟迪, 张舒婷, 吴波. 《中国急性缺血性脑卒中诊治指南2018》解读[J]. 中国现代神经疾病杂志, 2019, 19(11): 897-901.

[14]

海英. 眼针治疗中风病疗效的中医学机理探析[J]. 中华中医药学刊, 2014, 32(10): 2453-2456.

[15]

国家市场监督管理总局, 国家标准化管理委员会. 针灸技术操作规范第15部分: 眼针: GB/T 21709.15—2021[S]. 北京: 中国标准出版社, 2021.

[16]

KANEHISA M, FURUMICHI M, TANABE M, et al. KEGG: new perspectives on genomes, pathways, diseases and drugs[J]. Nucleic Acids Res, 2017, 45(D1): D353-D361.

[17]

GABALDÓN T, KOONIN E V. Functional and evolutionary implications of gene orthology[J]. Nat Rev Genet, 2013, 14(5): 360-366.

[18]

SNEHA P, THIRUMAL KUMAR D, LIJO J, et al. Probing the protein-protein interaction network of proteins causing maturity onset diabetes of the young[J]. Adv Protein Chem Struct Biol, 2018, 110: 167-202.

[19]

YU G C, WANG L G, HAN Y Y, et al. clusterProfiler: an R package for comparing biological themes among gene clusters[J]. OMICS, 2012, 16(5): 284-287.

[20]

QUICKE D, BUTCHER B A, WELTON R K. Principal components analysis[M]. Practical R for biologists: an introduction. UK: CA-BI, 2021: 194-199.

[21]

刘昱麟, 腾飞, 陈宇, 等. 眼针对CIRI大鼠的抗炎及促进血管新生作用机制的实验研究[J]. 时珍国医国药, 2021, 32(11): 2784-2788.

[22]

徐畅, 马贤德, 刘昱麟, 等. 眼针对急性脑缺血再灌注损伤模型大鼠CREB和BDNF蛋白表达的影响[J]. 中华中医药学刊, 2021, 39(2): 13-16.

[23]

何树诺, 韩晓伟, 潘茜, 等. 眼针调控转录激活因子6通路改善急性脑缺血再灌注损伤大鼠自噬的机制研究[J]. 针刺研究, 2022, 47(10): 859-865.

[24]

徐畅, 邰东梅, 马贤德, 等. 眼针和体针对24h脑缺血再灌注损伤模型大鼠脑血流和脑源性神经营养因子表达的影响[J]. 中华中医药学刊, 2019, 37(9): 2178-2181.

[25]

魏伟. 炎症免疫反应软调节[J]. 中国药理学通报, 2016, 32(3): 297-303.

[26]

李雪丽, 刘钊, 于博文, 等. 缺血性脑卒中免疫炎症反应机制的研究进展[J]. 中华疾病控制杂志, 2021, 25(3): 352-358.

[27]

IADECOLA C, BUCKWALTER M S, ANRATHER J. Immune responses to stroke: mechanisms, modulation, and therapeutic potential[J]. J Clin Invest, 2020, 130(6): 2777-2788.

[28]

XU S B, LU J N, SHAO A W, et al. Glial cells: role of the immune response in ischemic stroke[J]. Front Immunol, 2020, 11: 294.

[29]

NIKOLIC D, JANKOVIC M, PETROVIC B, et al. Genetic aspects of inflammation and immune response in stroke[J]. Int J Mol Sci, 2020, 21(19): 7409.

[30]

WU F, LIU Z C, ZHOU L H, et al. Systemic immune responses after ischemic stroke: From the center to the periphery[J]. Front Immunol, 2022, 13: 911661.

[31]

WATTERS O, O'CONNOR J J. A role for tumor necrosis factor-α in ischemia and ischemic preconditioning[J]. J Neuroinflammation, 2011, 8: 87.

[32]

LIU T, CLARK R K, MCDONNELL P C, et al. Tumor necrosis factor-alpha expression in ischemic neurons[J]. Stroke, 1994, 25(7): 1481-1488.

[33]

朱芸燕, 张传武, 陈靖, 等. 远端缺血预处理对急性缺血性脑卒中炎症反应的作用及机制研究进展[J]. 中风与神经疾病杂志, 2024, 41(1): 35-40.

[34]

杨士成, 海英. 不同针刺手法对脑缺血再灌注大鼠眼针穴区肥大细胞活化作用的研究[J]. 中华中医药学刊, 2024, 42(12): 99-103, 后插40.

[35]

MALIK A, KANNEGANTI T D. Function and regulation of IL-1α in inflammatory diseases and cancer[J]. Immunol Rev, 2018, 281(1): 124-137.

[36]

VAN DEN EECKHOUT B, TAVERNIER J, GERLO S. Interleukin-1 as innate mediator of T cell immunity[J]. Front Immunol, 2021, 11: 621931.

[37]

贺树成, 李运曼, 方伟蓉. CCR2/CCL2对脑缺血再灌注损伤影响的研究进展[J]. 药学与临床研究, 2018, 26(3): 196-201.

[38]

ROBERTS T K, EUGENIN E A, LOPEZ L, et al. CCL2 disrupts the adherens junction: implications for neuroinflammation[J]. Lab Invest, 2012, 92(8): 1213-1233.

[39]

HE M F, DONG H Q, HUANG Y H, et al. Astrocyte-derived CCL2 is associated with M1 activation and recruitment of cultured microglial cells[J]. Cell Physiol Biochem, 2016, 38(3): 859-870.

2026年第44卷第9期

PDF下载

引用本文

BibTeX

文章信息

doi: 10.13193/j.issn.1673-7717.2026.09.024

首发时间：2026-10-20
出版时间：2026-09-10

补充材料

相关文章

文章信息

作者

出版历史

基金

国家中医药管理局田维柱全国名中医传承工作室项目(国中医药办人教函〔2022〕245号)

海英辽宁省名中医传承工作室(辽卫发〔2021〕7号)

辽宁省“兴辽英才计划”项目(XLYC2002104)

作者信息

辽宁中医药大学附属医院，辽宁　沈阳　110032

通讯作者:

海英（1969-），女（蒙古族），辽宁阜新人，教授、主任医师，博士研究生导师，博士，研究方向：针灸治疗神经系统疾病。

参考文献

分享链接

https://castjournals.cast.org.cn/joweb/zhzyyxk/CN/10.13193/j.issn.1673-7717.2026.09.024

分享至

全文二维码

扫描看全文

引用本文

BibTeX

本文的引用情况

2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

关闭全屏

BibTeX
EndNote
RefWorks
TxT