中华中医药学刊

中药单体桑白皮总黄酮介导TGF-β1/STAT3/NF-κB信号通路调控中耳炎大鼠炎症反应的影响

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王莉竹 , 杨艳丽 , 李书聆 , 阮标

中华中医药学刊 | 国家项目点击 2026,44(9): 65-68

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中华中医药学刊 |国家项目点击 2026 , 44 (9) : 65 -68

中药单体桑白皮总黄酮介导TGF-β1/STAT3/NF-κB信号通路调控中耳炎大鼠炎症反应的影响

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王莉竹, 杨艳丽, 李书聆, 阮标

作者信息

云南大学附属医院，云南　昆明　650000

通讯作者:

阮标（1964-），男，云南昆明人，主任医师，博士，研究方向：耳疾病。E-mail：983444635@qq.com。

作者简介:

王莉竹（1991-），女，云南保山人，主治医师，硕士，研究方向：耳疾病。

Effect of Total Flavonoids of Sangbaipi (Mori Cortex) on Regulation of Inflammatory Response in Rats with Otitis Media Through TGF-β1/STAT3/NF-κB Signaling Pathway

Lizhu WANG, Yanli YANG, Shuling LI, Biao RUAN

Affiliations

The Affiliated Hospital of Yunnan University, Kunming 650000, Yunnan, China

出版时间: 2026-09-10 doi: 10.13193/j.issn.1673-7717.2026.09.012

文章导航

摘要

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目的

探讨中药单体桑白皮总黄酮介导转化生长因子-β1（transforming growth factor-β1，TGF-β1）/信号转导和转录激活因子3（signal transducer and activator of transcription3，STAT3）/核因子-κB（nuclear factor-κB，NF-κB）信号通路调控中耳炎大鼠炎症反应的影响。

方法

SPF级SD大鼠40只，随机分为正常组、模型组、低剂量组和高剂量组，每组10只。正常组和模型组给予0.9%氯化钠溶液等剂量灌胃；低剂量组给予10 mg/kg桑白皮总黄酮，1次/d；高剂量组给予30 mg/kg桑白皮总黄酮，1次/d。各组大鼠连续灌胃14 d。观察各组大鼠苏木精-伊红染色法（hematoxylin-eosin，HE）染色组织病理变化；比较各组大鼠鼓膜病理学评分、听功能、中耳黏膜厚度、炎症因子及TGF-β1水平、STAT3和NF-κB蛋白表达。

结果

模型组、低剂量组和高剂量组大鼠鼓膜病理学评分、ABR反应阈值、中耳黏膜厚度、血清白细胞介素（interleukin，IL）-1β、IL-6和TGF-β1水平、STAT3和NF-κB蛋白灰度值高于正常组（P<0.05）；低剂量组和高剂量组大鼠鼓膜病理学评分、听觉脑干诱发电位（Auditory Brainstem Response，ABR）反应阈值、中耳黏膜厚度、血清IL-1β、IL-6和TGF-β1水平、STAT3和NF-κB蛋白灰度值低于模型组（P<0.05）；高剂量组大鼠鼓膜病理学评分、ABR反应阈值、中耳黏膜厚度、血清IL-1β、IL-6和TGF-β1水平、STAT3和NF-κB蛋白灰度值低于低剂量组（P<0.05）。

结论

中药单体桑白皮总黄酮可减轻中耳炎大鼠炎症反应，其机制可能与调控TGF-β1/STAT3/NF-κB信号通路表达有关。

关键词

桑白皮总黄酮 / 转化生长因子-β1 / 信号转导和转录激活因子3 / 核因子-κB / 中耳炎 / 炎症反应

Abstract

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Objective

To investigate the effect of total flavonoids of Sangbaipi (Mori Cortex), a traditional Chinese medicine monomer, on the regulation of inflammatory response in the rats with otitis media through the transforming growth factor-β1 (TGF-β1)/signal transducer and activator of transcription3 (STAT3)/nuclear factor-κB (NF-κB) signaling pathway.

Method

Forty SPF grade SD rats were randomly divided into a normal group, a model group, a low-dose group of total flavonoids of Sangbaipi (Mori Cortex) and a high-dose group of total flavonoids of Sangbaipi (Mori Cortex), each with 10 rats. Normal group and model group were given the equal doses of 0.9% sodium chloride solution by gavage. The low and high-dose groups of total flavonoids of Sangbaipi (Mori Cortex) were given the corresponding doses (10, 30 mg/kg) once a day. The rats in each group were orally administered continuously for 14 days. The histopathological changes of hematoxylin-eosin (HE) stained tissues in each group were observed. The pathological scores of the tympanic membrane, auditory function, thickness of the middle ear mucosa and the protein expression levels of inflammatory factors and TGF-β1, STAT3 and NF-κB among different groups were compared.

Result

In the model group, low-dose group and high-dose group, the pathological scores of the tympanic membrane, Auditory Brainstem Response (ABR) response threshold, middle ear mucosal thickness, serum levels of interleukin (IL)-1β, IL-6 and TGF-β1, as well as the grayscale values of STAT3 and NF-κB proteins were higher than those in the normal group (P<0.05). The pathological scores of the tympanic membrane, ABR response threshold, middle ear mucosal thickness, serum levels of IL-1β, IL-6 and TGF-β1, as well as the grayscale values of STAT3 and NF-κB proteins in the low-dose and high-dose groups were lower than those of the model group (P<0.05). The pathological score of the tympanic membrane, Auditory Brainstem Response (ABR) response threshold, thickness of the middle ear mucosa, serum levels of IL-1β, IL-6, and TGF-β1, as well as the gray values of STAT3 and NF-κB proteins in the high-dose group lower than those in the low-dose group (P<0.05).

Conclusion

The total flavonoids of Sangbaipi (Mori Cortex), a traditional Chinese medicine monomer, can alleviate the inflammatory response in the rats with otitis media, and its mechanism may be related to the regulation of TGF-β1/STAT3/NF-κB signaling pathway expression.

Key words

total flavonoids of Sangbaipi (Mori Cortex) / transforming growth factor-β1 / signal transduction and transcription activator 3 / nuclear factor-kappa B / tympanitis / inflammatory reaction

引用本文

王莉竹, 杨艳丽, 李书聆, 阮标. 中药单体桑白皮总黄酮介导TGF-β1/STAT3/NF-κB信号通路调控中耳炎大鼠炎症反应的影响. 中华中医药学刊, 2026 , 44 (9) : 65 -68 . DOI: 10.13193/j.issn.1673-7717.2026.09.012

Lizhu WANG, Yanli YANG, Shuling LI, Biao RUAN. Effect of Total Flavonoids of Sangbaipi (Mori Cortex) on Regulation of Inflammatory Response in Rats with Otitis Media Through TGF-β1/STAT3/NF-κB Signaling Pathway[J]. Chinese Archives of Traditional Chinese Medicine, 2026 , 44 (9) : 65 -68 . DOI: 10.13193/j.issn.1673-7717.2026.09.012

基金

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国家重点研发计划中医药现代化研究重点专项(2021YFC1106420)

参考文献

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李海亮, 王海茹, 吴艳艳. 宣肺通窍汤对分泌性中耳炎大鼠的作用及与TLR4/MyD88/NF-κB通路相关机制探讨[J]. 长春中医药大学学报, 2023, 39(4): 395-399.

2026年第44卷第9期

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doi: 10.13193/j.issn.1673-7717.2026.09.012

首发时间：2026-10-20
出版时间：2026-09-10

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国家重点研发计划中医药现代化研究重点专项(2021YFC1106420)

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云南大学附属医院，云南　昆明　650000

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阮标（1964-），男，云南昆明人，主任医师，博士，研究方向：耳疾病。E-mail：983444635@qq.com。

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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