中华中医药学刊

大黄玄明粉冲剂通过PI3K/AKT/NF-κB信号传导和改善肠道微生物群改善急性重症胰腺炎诱导的肠屏障损伤

PDF下载

黄广文 ¹ , 何锦雄 ¹ , 温玉平 ¹ , 黄海阳 ² , 周正 ¹

中华中医药学刊 | 国家项目点击 2026,44(9): 59-64

收起

中华中医药学刊 |国家项目点击 2026 , 44 (9) : 59 -64

大黄玄明粉冲剂通过PI3K/AKT/NF-κB信号传导和改善肠道微生物群改善急性重症胰腺炎诱导的肠屏障损伤

全屏

黄广文¹, 何锦雄¹, 温玉平¹, 黄海阳², 周正¹

作者信息

^1.广州中医药大学东莞医院，广东　东莞　523000

^2.广州中医药大学东莞医院中医药研究所，广东　东莞　523000

通讯作者:

周正（1972-），男，湖北武汉人，主任医师，学士，研究方向：中西医结合治疗脾胃肝胆疾病。E-mail：zhouzhengdg@163.com。

作者简介:

黄广文（1983-），男，广东东莞人，主任医师，学士，研究方向：中西医结合治疗脾胃肝胆疾病。

Dahuang Xuanming Powder Granules (大黄玄明粉) Improves Intestinal Barrier Injury Induced by Severe Acute Pancreatitis Through PI3K/AKT/NF-κB Signal Transduction and Improvement of Intestinal Microflora

Guangwen HUANG¹, Jinxiong HE¹, Yuping WEN¹, Haiyang HUANG², Zheng ZHOU¹

Affiliations

^1.Dongguan Hospital of Guangzhou University of Chinese Medicine, Dongguan 523000, Guangdong, China

^2.Institute of Traditional Chinese Medicine, Dongguan Hospital, Guangzhou University of Chinese Medicine, Dongguan 523000, Guangdong, China

出版时间: 2026-09-10 doi: 10.13193/j.issn.1673-7717.2026.09.011

文章导航

摘要

收起

目的

探讨大黄玄明粉冲剂通过磷脂酰肌醇3-激酶（phosphatidylinositol 3-kinase，PI3K）/蛋白激酶B（protein kinase B，AKT）/核因子κ B（nuclear factor kappa B，NF-κB）对胰腺炎制备的肠屏障损伤的保护作用。

方法

采用5%（m/V，50 mg/mL）牛磺胆酸钠溶液0.2 mL/只，经胆胰总管逆行注射建立Sprague-Dawley（SD）大鼠重症急性胰腺炎（severe acute pancreatitis，SAP）模型。实验分为假手术组、SAP致肠屏障损伤组（模型组）和大黄玄明粉冲剂组。通过扫描电镜、苏木精和伊红染色和病理评分评估肠屏障损伤。测量血清或肠道组织中胰腺炎生物标志物（淀粉酶和脂肪酶）、氧化应激标志物[超氧化物歧化酶（superoxidase dismutase，SOD）、过氧化氢酶（catalase，CAT）、谷胱甘肽过氧化物酶（glutathione peroxidases，GPx）和丙二醛（malondialdehyde，MDA）]，以及炎症标志物[肿瘤坏死因子α（tumor necrosis factorα，TNF-α）、白细胞介素（interleukin，IL）-1β、IL-6和IL-10]的水平。蛋白质免疫印迹法检测PI3K/AKT/NF-κB信号通路相关蛋白的表达。使用16 S rRNA测序来测量粪便中的肠道微生物群组成。

结果

模型组血清淀粉酶和脂肪酶的活性高于假手术组。大黄玄明粉冲剂组血清淀粉酶和脂肪酶活性低于模型组。与假手术组相比，模型组的肠绒毛数量减少并受损，而大黄玄明粉冲剂治疗阻止了肠绒毛数量的减少。此外，大黄玄明粉冲剂治疗提高了血清SOD、CAT和GSPx的活性，降低血清MDA水平。与模型组相比，大黄玄明粉冲剂治疗降低TRL4、p-PI3K、p-AKT和p-NF-κB的蛋白水平。与模型组相比，大黄玄明粉冲剂治疗降低血清TNF-α、IL-1β和IL-6水平，但血清IL-10水平显著提高。此外，大黄玄明粉冲剂治疗增加了模型中乳酸杆菌和普雷沃氏菌的比例，降低幽门螺杆菌和埃希氏菌属-志贺氏菌的比例。

结论

大黄玄明粉冲剂通过失活氧化剂和炎症信号并改善肠道微生物群，最终改善SAP诱导的大鼠肠道屏障损伤。

关键词

大黄玄明粉冲剂 / PI3K/AKT/NF-κB信号通路 / 肠道微生物群 / 急性重症胰腺炎 / 肠屏障损伤

Abstract

收起

Objective

To investigate the protective effect of Dahuang Xuanming Powder Granules (大黄玄明粉) on intestinal barrier injury induced by pancreatitis through phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT)/nuclear factor kappa B (NF-κB).

Methods

By injection of 0.2 mL of a 5% (w/v, 50 mg/mL) sodium taurocholate solution to induce a Sprague-Dawley (SD) rat model with severe acute pancreatitis (SAP). The experiment was divided into sham operation group, SAP-induced intestinal barrier injury group (model group) and Dahuang Xuanming Powder Granules group. Intestinal barrier injury was assessed by scanning electron microscopy, hematoxylin and eosin staining and pathological scoring. The levels of pancreatitis biomarkers (amylase and lipase), oxidative stress markers [superoxide dismutase (SOD), catalase (CAT), glutathione peroxidases (GPx) and malondialdehyde (MDA)] and inflammatory markers [tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-6 and IL-10] in serum or intestinal tissue were measured. The expressions of phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT)/nuclear factor kappa-B (NF-κB) signaling pathway-related proteins were detected by Western blotting. 16 S rRNA sequencing was used to measure the composition of gut microbiota in feces.

Result

The activity of serum amylase and lipase in the model group was higher than that in the control group. The activity of serum amylase and lipase in Dahuang Xuanming Powder Granules group was lower than that in the model group. Compared with that in the control group, the number of intestinal villi in the model group decreased. The activity of serum amylase and lipase in the model group was higher than that in the control group. The activity of serum amylase and lipase in Dahuang Xuanming Powder Granules group was lower than that in the model group. Compared with that of the control group, the number of intestinal villi in the model group decreased and was damaged, while the treatment of Dahuang Xuanming Powder Granules prevented the reduction of the number of intestinal villi. In addition, Dahuang Xuanming Powder Granules increased the activity of the levels of serum SOD, CAT and GSPx, and decreased the serum MDA levels. Compared with the model group, Dahuang Xuanming Powder Granules reduced the protein levels of Toll like receptor 4 (TRL4), p-PI3K, p-AKT and p-NF-κB. Compared with the model group, Dahuang Xuanming Powder Granules reduced the levels of serum TNF-α, IL-1β and IL-6, but significantly increased serum IL-10 levels. In addition, Dahuang Xuanming Powder Granules increased the proportion of Lactobacillus and Prevotella in the model group, and reduced the proportion of Helicobacter pylori and Escherichia-Shigella.

Conclusion

By inactivating oxidants and inflammatory signals and improving intestinal microbiota, Dahuang Xuanming Powder Granules ultimately improve SAP-induced intestinal barrier injury in the rats.

Key words

Dahuang Xuanming Powder Granules (大黄玄明粉冲剂) / PI3K/AKT/NF-κB pathway / gut microbiota / severe acute pancreatiti / intestinal barrier injury

引用本文

黄广文, 何锦雄, 温玉平, 黄海阳, 周正. 大黄玄明粉冲剂通过PI3K/AKT/NF-κB信号传导和改善肠道微生物群改善急性重症胰腺炎诱导的肠屏障损伤. 中华中医药学刊, 2026 , 44 (9) : 59 -64 . DOI: 10.13193/j.issn.1673-7717.2026.09.011

Guangwen HUANG, Jinxiong HE, Yuping WEN, Haiyang HUANG, Zheng ZHOU. Dahuang Xuanming Powder Granules (大黄玄明粉) Improves Intestinal Barrier Injury Induced by Severe Acute Pancreatitis Through PI3K/AKT/NF-κB Signal Transduction and Improvement of Intestinal Microflora[J]. Chinese Archives of Traditional Chinese Medicine, 2026 , 44 (9) : 59 -64 . DOI: 10.13193/j.issn.1673-7717.2026.09.011

基金

收起

国家自然科学基金面上项目(82274381)
广东省中医药局中医药科研项目(20221421)
东莞市卫生健康局“周正东莞市名中医药专家传承工作室”建设项目(东卫函〔2020〕136号)
2022年东莞市社会发展科技项目(20221800900032)

参考文献

收起

文献

收起

参考文献引证文献

排序方式：

[1]

PORTELLI M, JONES C D. Severe acute pancreatitis: pathogenesis, diagnosis and surgical management[J]. Hepatobiliary Pancreat Dis Int, 2017, 16(2): 155-159.

[2]

SHAH A P, MOURAD M M, BRAMHALL S R. Acute pancreatitis: current perspectives on diagnosis and management[J]. J Inflamm Res, 2018, 11: 77-85.

[3]

ZHAI Y Z, GAN L, HUANG S, et al. Therapeutic effect of ultrasound interventional perirenal catheter-assisted early peripancreatic lavage of protease inhibitor on severe acute pancreatitis in miniature pigs[J]. Pancreatology, 2019, 19(1): 158-162.

[4]

向斌, 张国培, 崔华永, 等. 大黄鼻饲对重症胰腺炎腹腔感染的疗效及对血管内皮糖萼的影响[J]. 中国老年学杂志, 2021, 41(22): 4943-4946.

[5]

高翔, 刘梅梅, 姚琪琪, 等. 大承气汤联合奥曲肽治疗急性重症胰腺炎的临床研究[J]. 中华中医药学刊, 2023(2): 238-242.

[6]

李君秋, 戴彦成, 曹红燕, 等. 大承气汤能够通过调节肠道微生物群抑制轻症急性胰腺炎患者炎症反应并促进胃肠功能恢复[J]. 中华危重病急救医学, 2023, 35(2): 170-176.

[7]

MA R, YUAN F, WANG S X, et al. Calycosin alleviates ceruleininduced acute pancreatitis by inhibiting the inflammatory response and oxidative stress via the p38 MAPK and NF-κB signal pathways in mice[J]. Biomed Pharmacother, 2018, 105: 599-605.

[8]

XIE Q, FEI M M, FA Z Z, et al. Methane-rich saline alleviates cerulein-induced acute pancreatitis by inhibiting inflammatory response, oxidative stress and pancreatic apoptosis in mice[J]. Int Immunopharmacol, 2017, 51: 17-24.

[9]

PIAO X H, LIU B H, GUO L Y, et al. Picroside II shows protective functions for severe acute pancreatitis in rats by preventing NF-κB-dependent autophagy[J]. Oxid Med Cell Longev, 2017, 2017: 7085709.

[10]

LI X Y, HE C, ZHU Y, et al. Role of gut microbiota on intestinal barrier function in acute pancreatitis[J]. World J Gastroenterol, 2020, 26(18): 2187-2193.

[11]

ZHAO M Q, CUI M Y, JIANG Q L, et al. Profile of pancreatic and ileal microbiota in experimental acute pancreatitis[J]. Microorganisms, 2023, 11(11): 2707.

[12]

ZHANG D L, ZHU C L, FANG Z Q, et al. Remodeling gut microbiota by Clostridium butyricum (C. butyricum) attenuates intestinal injury in burned mice[J]. Burns, 2020, 46(6): 1373-1380.

[13]

LI C L, AI G X, WANG Y F, et al. Oxyberberine, a novel gut microbiota-mediated metabolite of berberine, possesses superior anticolitis effect: Impact on intestinal epithelial barrier, gut microbiota profile and TLR4-MyD88-NF-κB pathway[J]. Pharmacol Res, 2020, 152: 104603.

[14]

JIANG K F, GUO S, YANG C, et al. Barbaloin protects against lipopolysaccharide (LPS) -induced acute lung injury by inhibiting the ROS-mediated PI3K/AKT/NF-κB pathway[J]. Int Immunopharmacol, 2018, 64: 140-150.

[15]

THUMMAYOT S, TOCHARUS C, JUMNONGPRAKHON P, et al. Cyanidin attenuates Aβ(25-35)-induced neuroinflammation by suppressing NF-κB activity downstream of TLR4/NOX4 in human neuroblastoma cells[J]. Acta Pharmacol Sin, 2018, 39(9): 1439-1452.

[16]

AHO H J, NEVALAINEN T J, LINDBERG R L, et al. Experimental pancreatitis in the rat. The role of phospholipase A in sodium taurocholate-induced acute haemorrhagic pancreatitis[J]. Scand J Gastroenterol, 1980, 15(8): 1027-1031.

[17]

LUAN Z G, ZHANG J, YIN X H, et al. Ethyl pyruvate significantly inhibits tumour necrosis factor-α, interleukin-1β and high mobility group box 1 releasing and attenuates sodium taurocholate-induced severe acute pancreatitis associated with acute lung injury[J]. Clin Exp Immunol, 2013, 172(3): 417-426.

[18]

SHI Q, LIAO K S, ZHAO K L, et al. Hydrogen-rich saline attenuates acute renal injury in sodium taurocholate-induced severe acute pancreatitis by inhibiting ROS and NF-κB pathway[J]. Mediators Inflamm, 2015, 2015: 685043.

[19]

李志文. 中西医结合治疗急性胰腺炎[J]. 吉林中医药, 2004, 24(8): 39.

[20]

王子孺, 李贺. 大黄素通过NF-κB-iNOS/COX-2信号通路治疗急性胰腺炎的作用机制研究[J]. 肝胆外科杂志, 2023, 31(3): 224-228, 240.

[21]

RAO C Y, FU L Y, HU C L, et al. H₂S mitigates severe acute pancreatitis through the PI3K/AKT-NF-κB pathway in vivo[J]. World J Gastroenterol, 2015, 21(15): 4555-4563.

[22]

XIA S L, NI Y J, ZHOU Q, et al. Emodin attenuates severe acute pancreatitis via antioxidant and anti-inflammatory activity[J]. Inflammation, 2019, 42(6): 2129-2138.

[23]

XIONG J, WANG K Z, YUAN C X, et al. Luteolin protects mice from severe acute pancreatitis by exerting HO-1-mediated anti-inflammatory and antioxidant effects[J]. Int J Mol Med, 2017, 39(1): 113-125.

[24]

XU T Y, CHEN Y, YU L F, et al. Effects of Lactobacillus plantarum on intestinal integrity and immune responses of egg-laying chickens infected with Clostridium perfringens under the free-range or the specific pathogen free environment[J]. BMC Vet Res, 2020, 16(1): 47.

[25]

CHEN Y J, GUO K M, NAGY T, et al. Chronic oral exposure to glycated whey proteins increases survival of aged male NOD mice with autoimmune prostatitis by regulating the gut microbiome and antiinflammatory responses[J]. Food Funct, 2020, 11(1): 153-162.

[26]

PERNA A, HAY E, CONTIERI M, et al. Adherent-invasive Escherichia coli (AIEC): Cause or consequence of inflammation, dysbiosis, and rupture of cellular joints in patients with IBD?[J]. J Cell Physiol, 2020, 235(6): 5041-5049.

[27]

FACHI J L, DE SOUZA FELIPE J, PRAL L P, et al. Butyrate protects mice from clostridium difficile-induced colitis through an HIF-1-dependent mechanism[J]. Cell Rep, 2019, 27(3): 750-761. e7.

[28]

LIU L, ZHANG T, SUI Y H, et al. Gut microbiota affects pancreatic fibrotic progression through immune modulation in chronic pancreatitis[J]. Microb Pathog, 2023, 177: 106035.

[29]

SHAIKH S D, SUN N, CANAKIS A, et al. Irritable bowel syndrome and the gut microbiome: a comprehensive review[J]. J Clin Med, 2023, 12(7): 2558.

[30]

吴萌萌. 重症急性胰腺炎大鼠结肠粘液层微生物群变化及大黄附子汤干预作用[D]. 大连: 大连大学, 2021.

[31]

宋轶, 唐远莉, 路晓光, 等. 大黄附子汤对重症急性胰腺炎早期大鼠结肠黏液层作用的研究[J]. 中华实验外科杂志, 2023, 40(1): 35-39.

2026年第44卷第9期

PDF下载

引用本文

BibTeX

文章信息

doi: 10.13193/j.issn.1673-7717.2026.09.011

首发时间：2026-10-20
出版时间：2026-09-10

补充材料

相关文章

文章信息

作者

出版历史

基金

国家自然科学基金面上项目(82274381)

广东省中医药局中医药科研项目(20221421)

东莞市卫生健康局“周正东莞市名中医药专家传承工作室”建设项目(东卫函〔2020〕136号)

2022年东莞市社会发展科技项目(20221800900032)

作者信息

^1.广州中医药大学东莞医院，广东　东莞　523000

^2.广州中医药大学东莞医院中医药研究所，广东　东莞　523000

通讯作者:

周正（1972-），男，湖北武汉人，主任医师，学士，研究方向：中西医结合治疗脾胃肝胆疾病。E-mail：zhouzhengdg@163.com。

参考文献

分享链接

https://castjournals.cast.org.cn/joweb/zhzyyxk/CN/10.13193/j.issn.1673-7717.2026.09.011

分享至

全文二维码

扫描看全文

引用本文

BibTeX

本文的引用情况

2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

关闭全屏

BibTeX
EndNote
RefWorks
TxT