中华中医药学刊

定量蛋白质组学探讨菟丝子黄酮对雷公藤多苷致雄鼠生殖毒性的影响机制

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孙利辉 ¹^,² , 丁樱 ¹^,² , 韩姗姗 ¹^,² , 代彦林 ¹ , 徐闪闪 ¹ , 高敏 ¹

中华中医药学刊 | 国家项目点击 2026,44(7): 117-123

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中华中医药学刊 |国家项目点击 2026 , 44 (7) : 117 -123

定量蛋白质组学探讨菟丝子黄酮对雷公藤多苷致雄鼠生殖毒性的影响机制

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孙利辉¹^,², 丁樱¹^,², 韩姗姗¹^,², 代彦林¹, 徐闪闪¹, 高敏¹

作者信息

^1.河南中医药大学第一附属医院儿科医院，河南　郑州　450000

^2.河南中医药大学儿科医学院，河南　郑州　450046

通讯作者:

丁樱（1951-），女，江苏南京人，国医大师，主任医师、教授，博士研究生导师，学士，研究方向：中医药防治小儿肾病及风湿免疫性疾病。E-mail：dingying3236@sina.com。

作者简介:

孙利辉（1998-），女，河南洛阳人，博士在读，研究方向：中医药防治小儿肾病及风湿免疫性疾病。

Exploring Effect of Tusizi (Cuscuta chinensis) Flavonoids on Reproductive Toxicity of Leigongtong (Tripterygium wilfordii) Glycosides in Male Rats Based on Quantitative Proteomics

Lihui SUN¹^,², Ying Ding¹^,², Shanshan HAN¹^,², Yanlin DAI¹, Shanshan XU¹, Min GAO¹

Affiliations

^1.Pediatric Hospital of The First Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou 450000, Henan, China

^2.Pediatric Medical College of Henan University of Chinese Medicine, Zhengzhou 450046, Henan, China

出版时间: 2026-07-10 doi: 10.13193/j.issn.1673-7717.2026.07.022

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摘要

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目的

基于串联质量标签（Tandem mass tags，TMT）定量蛋白质组学和生物信息学方法探讨菟丝子黄酮（Cuscuta chinensis flavonoids，CCF）改善雷公藤多苷（Tripterygium wilfordii glycosides，GTW）致雄鼠生殖毒性的作用机制。

方法

将21只雄性SD大鼠随机分为空白组、GTW模型组、CCF治疗组，分别灌胃相应药物，连续12周，实验结束后进行苏木精-伊红染色以评价CCF对GTW致生殖毒性的改善作用。利用TMT定量蛋白质组学技术来评估从睾丸组织中提取的蛋白质，通过生物信息学方法对差异表达的蛋白质进行分析，蛋白免疫印迹法验证蛋白质组学筛选出的关键差异表达蛋白。

结果

菟丝子黄酮能显著改善大鼠睾丸组织的病理损伤，干预后共检测到29种差异表达的蛋白质，包括6种上调蛋白质和23种下调蛋白质。京都基因与基因组百科全书（Kyoto Encyclopedia of Genes and Genomes，KEGG）通路富集分析显示，非洲锥虫病、补体与凝血级联系统、生物合成安沙霉素、组氨酸代谢、维生素消化吸收是CCF改善GTW生殖毒性的主要途径。而基因本体（Gene Ontology，GO）分析显示，CCF改善GTW生殖毒性的差异表达蛋白主要与胶原蛋白、血浆脂蛋白、蛋白质氧化、凝血内在途径、肝素结合相关。COG注释分析显示，防御机制、细胞外结构与CCF改善GTW生殖毒性的机制密切联系。菟丝子黄酮能使Apoa1、Tktl2上调，Hba1、Aldh3a1、Pzp、Serpind1下调。

结论

菟丝子黄酮可通过调控非洲锥虫病、补体与凝血级联系统、生物合成安沙霉素、组氨酸代谢、维生素消化吸收通路介导的胶原合成、脂质代谢、细胞凋亡、氧化应激等来减轻雷公藤多苷的睾丸毒性。

关键词

雷公藤多苷 / 菟丝子黄酮 / 生殖毒性 / 定量蛋白质组学

Abstract

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Objective

To explore the mechanism by which Tusizi (Cuscuta chinensis) flavonoids (CCF) improves the reproductive toxicity of Leigongtong (Tripterygium wilfordii) glycosides (GTW) in the male rats using Tandem Mass Tags (TMT) quantitative proteomics and bioinformatics methods.

Method

Twenty-one male SD rats were randomly divided into a control group, a GTW model group and a CCF treatment group, and were orally administered with the corresponding drugs for 12 consecutive weeks. After the experiment, hematoxylin eosin staining was performed to evaluate the improvement effect of CCF on GTW-induced reproductive toxicity. TMT quantitative proteomics technology was used to evaluate proteins extracted from testicular tissue, analyzing differentially expressed proteins through bioinformatics methods and verifying key differentially expressed proteins screened by proteomics through western blot.

Result

CCF can significantly improving the pathological damage of the testicular tissues of the rats. After intervention, a total of 29 differentially expressed proteins were detected, including 6 up-regulated proteins and 23 down-regulated proteins. KEGG pathway analysis showed that African trypanosomiasis, complement coagulation cascade system, biosynthesis of amphotericin, histidine metabolism and vitamin digestion and absorption were the main pathways by which CCF improved the reproductive toxicity of GTW. GO analysis showed that the differentially expressed proteins in CCF improving GTW reproductive toxicity were mainly related to collagen, plasma lipoprotein, protein oxidation, intrinsic coagulation pathways and heparin binding. COG annotation analysis showed that defense mechanisms, extracellular structures and the mechanisms by which CCF improved GTW reproductive toxicity were closely related. Dodder seed flavonoids can up-regulate the expressions of Apoa1and Tktl2, and down-regulate the expressions of Hba1, Aldh3a1, Pzp and Serpind1.

Conclusion

CCF can alleviate the testicular toxicity of GTW by regulating collagen synthesis, lipid metabolism, cell apoptosis and oxidative stress mediated by African trypanosomiasis, complement and coagulation cascade system, biosynthesis of ansamycins, histidine metabolism, vitamin digestion and absorption pathway.

Key words

Leigongtong (Tripterygium wilfordii) polyglycosides / Tusizi (Cuscuta chinensis) flavonoids / reproductive toxicity / quantitative proteomics

引用本文

孙利辉, 丁樱, 韩姗姗, 代彦林, 徐闪闪, 高敏. 定量蛋白质组学探讨菟丝子黄酮对雷公藤多苷致雄鼠生殖毒性的影响机制. 中华中医药学刊, 2026 , 44 (7) : 117 -123 . DOI: 10.13193/j.issn.1673-7717.2026.07.022

Lihui SUN, Ying Ding, Shanshan HAN, Yanlin DAI, Shanshan XU, Min GAO. Exploring Effect of Tusizi (Cuscuta chinensis) Flavonoids on Reproductive Toxicity of Leigongtong (Tripterygium wilfordii) Glycosides in Male Rats Based on Quantitative Proteomics[J]. Chinese Archives of Traditional Chinese Medicine, 2026 , 44 (7) : 117 -123 . DOI: 10.13193/j.issn.1673-7717.2026.07.022

基金

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国家自然科学基金青年科学基金项目(82205187)
河南省中医学“双一流”创建科学研究专项(HSRP-DFCTCM-2023-1-01)
河南中医药大学第一附属医院博士科研启动基金项目(2022BSJJ2005)

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2026年第44卷第7期

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doi: 10.13193/j.issn.1673-7717.2026.07.022

首发时间：2026-10-20
出版时间：2026-07-10

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基金

国家自然科学基金青年科学基金项目(82205187)

河南省中医学“双一流”创建科学研究专项(HSRP-DFCTCM-2023-1-01)

河南中医药大学第一附属医院博士科研启动基金项目(2022BSJJ2005)

作者信息

^1.河南中医药大学第一附属医院儿科医院，河南　郑州　450000

^2.河南中医药大学儿科医学院，河南　郑州　450046

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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