Article(id=1317115035848126750, tenantId=1146029695717560320, journalId=1255847919539208197, issueId=1317114999827452826, articleNumber=null, orderNo=null, doi=10.13193/j.issn.1673-7717.2026.06.025, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=null, receivedDateStr=null, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1791954295359, onlineDateStr=2026-10-14, pubDate=1781020800000, pubDateStr=2026-06-10, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1791954295359, onlineIssueDateStr=2026-10-14, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1791954295359, creator=13701087609, updateTime=1791954295359, updator=13701087609, issue=Issue{id=1317114999827452826, tenantId=1146029695717560320, journalId=1255847919539208197, year='2026', volume='44', issue='6', pageStart='1', pageEnd='258', issueExtLink='null', onlineDate='null', pubDate='1781020800000', pubDateStr='2026-06-10', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1791954286772, creator='13701087609', updateTime=1791958677365, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1317133415753831385, tenantId=1146029695717560320, journalId=1255847919539208197, issueId=1317114999827452826, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1317133415753831386, tenantId=1146029695717560320, journalId=1255847919539208197, issueId=1317114999827452826, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=140, endPage=145, ext={EN=ArticleExt(id=1317115036057841951, articleId=1317115035848126750, tenantId=1146029695717560320, journalId=1255847919539208197, language=EN, title=Effect of Modified Guishen Wan (归肾丸化裁) on Improving Inflammatory Reaction in PCOS Rats by Regulating TLR4/NF-κB Signal Pathway, columnId=1256183361521967297, journalTitle=Chinese Archives of Traditional Chinese Medicine, columnName=Target on National Project, runingTitle=null, highlight=null, articleAbstract=
Objective

To research the therapeutic effects of Modified Guishen Wan (归肾丸化裁) on rat model of polycystic ovary syndrome (PCOS) by regulating Toll-like receptor 4 (TLR4) /nuclear factor-κB (NF-κB) signaling pathway, particularly its impact on ovarian function, serum hormone levels and inflammatory factor expression.

Method

Forty 8-week-old SPF female SD rats with normal estrous cycle were randomly divided into four groups (n=10) : normal group, model group, Modified Guishen Wan group and positive drug group (metformin). Except for the normal group, the other groups were administered letrozole via gavage for 21 consecutive days to establish the PCOS model. The Modified Guishen Wan group and the positive drug group received the corresponding treatments for 21 days. After the experiment, the estrous cycle, ovarian index, serum sex hormone levels, serum inflammatory factor levels, the mRNA levels of inflammation-related genes in ovarian tissue and protein expression were detected to evaluate the efficacy of the Modified Guishen Wan.

Result

The estrous cycle of the normal group was 3-5 days/cycle. After 21 days of modeling, the estrous cycles of the other groups became disordered, with an average of 5-8 days/cycle, mostly in the interphase or prophase, and some lacked a complete estrous cycle, indicating ovarian damage. After 3 weeks of gavage treatment, the estrous cycles of the positive group and the Modified Guishen Wan group recovered. Compared with that of the normal group, the ovarian index of the model group significantly increased (P<0.01). Compared with that of the model group, the ovarian index of the rats of the Modified Guishen Wan group and the positive drug group significantly decreased (P<0.01), approaching the normal group levels. The ovarian tissue of the model group showed multiple cystic changes, with reduced or absent corpora lutea. In contrast, the Modified Guishen Wan group and the positive drug group had fewer cystic and atretic follicles, and the granulosa cell layer structure tended to be normal. The levels of testosterone (T) and luteinizing hormone (LH) in the model group were significantly higher (P<0.01, P<0.05), while follicle-stimulating hormone (FSH) levels were significantly lower (P<0.05). The Modified Guishen Wan group and the positive drug group could significantly reduce the levels of T and LH (P<0.05), and the Modified Guishen Wan group and the positive drug group could also significantly increase the FSH levels (P<0.05). Compared with those of the normal group, the serum levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1 β (IL-1β) in the model group were significantly elevated (P<0.001). Compared with the model group, both the Modified Guishen Wan and the positive drug could significantly reduce the serum levels of TNF-α, IL-6 and IL-1β in PCOS rats (P<0.01 and P<0.05). Compared with those of the normal group, the mRNA levels of IL-6, IL-1β, TNF-α and TLR4 in the ovarian tissue of the model group were significantly increased (P<0.05, P<0.01). The Modified Guishen Wan group had significantly lower levels of IL-6, IL-1β and TLR4 mRNA compared to the model group (P<0.05), with a trend toward lower TNF-αmRNA levels (P=0.188). Compared with the normal group, the protein expression of TLR4 in the ovarian tissue of the model group was significantly increased (P<0.05), and the phosphorylation levels of NF-κB p65 and IκBα were significantly elevated (P<0.05). The Modified Guishen Wan group had significantly lower TLR4 protein levels (P<0.05) and significantly reduced phosphorylation levels of NF-κB p65 and IκBα (P<0.05).

Conclusion

The Modified Guishen Wan may improve reproductive dysfunction and ovarian inflammation in PCOS rats by inhibiting the activation of the TLR4/NF-κB signaling pathway and reducing the production of inflammatory factors, providing new insights and theoretical basis for the clinical treatment of PCOS.

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目的

探究归肾丸化裁通过调控Toll样受体4(Toll-like receptor 4,TLR4)/核因子κB(nuclear factor kappa-B,NF-κB)信号通路对多囊卵巢综合征(polycystic ovary syndrome,PCOS)大鼠模型的治疗效果,特别是其对卵巢功能、血清激素水平及炎症因子表达的影响。

方法

选取动情周期正常的8周龄SPF级雌性SD大鼠40只,随机分为4组(n=10),分别为正常组、模型组、归肾丸化裁组、阳性药组(二甲双胍)。除正常组外,其余各组通过连续21 d给予来曲唑灌胃建立PCOS模型。归肾丸化裁组与阳性药组分别接受相应药物治疗21 d。实验结束后,通过检测大鼠动情周期、卵巢指数、血清性激素水平、血清炎症因子水平、卵巢组织炎症相关基因mRNA水平及蛋白表达情况,评估归肾丸化裁的疗效。

结果

正常组大鼠动情周期为3~5 d/次;造模21 d后,其余各组动情周期紊乱,平均5~8 d/次,多数处于动情间期或前期,甚至未见完整动情周期,表明大鼠卵巢受损。经过3周的灌胃治疗后,阳性药组及归肾丸化裁组动情周期恢复。与正常组相比,模型组大鼠卵巢指数显著增加(P<0.01),与模型组相比,归肾丸化裁组和阳性药组大鼠卵巢指数显著下降(P<0.01),接近正常组水平。模型组大鼠卵巢组织呈多囊性改变,黄体减少或消失,而归肾丸化裁组和阳性药组大鼠卵巢组织中囊性卵泡和闭锁性卵泡减少,颗粒细胞层结构趋向正常。模型组睾酮(testosterone,T)和促黄体生成素(luteinizing hormone,LH)水平显著升高(P<0.01,P<0.05),促卵泡激素(follicle-stimulating hormone,FSH)水平显著下降(P<0.05),而归肾丸化裁组和阳性药组能显著降低T和LH水平(P<0.05),归肾丸化裁组和阳性药组还能显著升高FSH水平(P<0.05)。与正常组相比,模型组血清肿瘤坏死因子-α(tumor necrosis factor-alpha,TNF-α)、白细胞介素-6(interleukin-6,IL-6)和白细胞介素-1 β(interleukin-1 beta,IL-1β)水平显著升高(P<0.001)。与模型组相比,归肾丸化裁组和阳性药组均能显著降低PCOS大鼠血清中TNF-α、IL-6和IL-1β水平(P<0.01和P<0.05)。与正常组相比,模型组卵巢组织中IL-6、IL-1β、TNF-α和TLR4 mRNA水平显著升高(P<0.05,P<0.01),归肾丸化裁组与模型组相比IL-6、IL-1β和TLR4 mRNA水平显著下降(P<0.05),TNF-αmRNA水平有降低趋势(P=0.188)。与正常组相比,模型组卵巢组织中TLR4蛋白表达显著升高(P<0.05),NF-κB p65和核因子κB抑制蛋白α(Inhibitor of NF-κB alpha,IκBα)磷酸化水平显著升高(P<0.05),与模型组相比,归肾丸化裁组TLR4蛋白水平显著下降(P<0.05),NF-κB p65和IκBα磷酸化水平显著下降(P<0.05)。

结论

归肾丸化裁可能通过抑制TLR4/NF-κB信号通路的激活,减少炎症因子的产生,进而改善PCOS大鼠的生殖功能障碍和卵巢炎症状态,为临床治疗PCOS提供了新的思路和理论依据。

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张晓芳(1981-),女,河南平顶山人,副主任中医师,硕士,研究方向:中医药治疗妇科常见病。

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叶利群(1973-),女,浙江宁波人,主任医师,硕士,研究方向:中医药治疗妇科常见病。E-mail:。
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归肾丸化裁通过调控TLR4/NF-κB信号通路改善PCOS大鼠炎症反应的研究
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张晓芳 , 沈颖 , 叶利群
中华中医药学刊 | 国家项目点击 2026,44(6): 140-145
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中华中医药学刊 |国家项目点击 2026 , 44 (6) : 140 -145
归肾丸化裁通过调控TLR4/NF-κB信号通路改善PCOS大鼠炎症反应的研究
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张晓芳, 沈颖, 叶利群
作者信息
  • 浙江中医药大学附属宁波市中医院,浙江 宁波 315010
通讯作者:
叶利群(1973-),女,浙江宁波人,主任医师,硕士,研究方向:中医药治疗妇科常见病。E-mail:。
作者简介:

张晓芳(1981-),女,河南平顶山人,副主任中医师,硕士,研究方向:中医药治疗妇科常见病。

Effect of Modified Guishen Wan (归肾丸化裁) on Improving Inflammatory Reaction in PCOS Rats by Regulating TLR4/NF-κB Signal Pathway
Xiaofang ZHANG, Ying SHEN, Liqun YE
Affiliations
  • Ningbo Traditional Chinese Medicine Hospital Affiliated to Zhejiang Chinese Medical University, Ningbo 315010, Zhejiang, China
出版时间: 2026-06-10 doi: 10.13193/j.issn.1673-7717.2026.06.025
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目的

探究归肾丸化裁通过调控Toll样受体4(Toll-like receptor 4,TLR4)/核因子κB(nuclear factor kappa-B,NF-κB)信号通路对多囊卵巢综合征(polycystic ovary syndrome,PCOS)大鼠模型的治疗效果,特别是其对卵巢功能、血清激素水平及炎症因子表达的影响。

方法

选取动情周期正常的8周龄SPF级雌性SD大鼠40只,随机分为4组(n=10),分别为正常组、模型组、归肾丸化裁组、阳性药组(二甲双胍)。除正常组外,其余各组通过连续21 d给予来曲唑灌胃建立PCOS模型。归肾丸化裁组与阳性药组分别接受相应药物治疗21 d。实验结束后,通过检测大鼠动情周期、卵巢指数、血清性激素水平、血清炎症因子水平、卵巢组织炎症相关基因mRNA水平及蛋白表达情况,评估归肾丸化裁的疗效。

结果

正常组大鼠动情周期为3~5 d/次;造模21 d后,其余各组动情周期紊乱,平均5~8 d/次,多数处于动情间期或前期,甚至未见完整动情周期,表明大鼠卵巢受损。经过3周的灌胃治疗后,阳性药组及归肾丸化裁组动情周期恢复。与正常组相比,模型组大鼠卵巢指数显著增加(P<0.01),与模型组相比,归肾丸化裁组和阳性药组大鼠卵巢指数显著下降(P<0.01),接近正常组水平。模型组大鼠卵巢组织呈多囊性改变,黄体减少或消失,而归肾丸化裁组和阳性药组大鼠卵巢组织中囊性卵泡和闭锁性卵泡减少,颗粒细胞层结构趋向正常。模型组睾酮(testosterone,T)和促黄体生成素(luteinizing hormone,LH)水平显著升高(P<0.01,P<0.05),促卵泡激素(follicle-stimulating hormone,FSH)水平显著下降(P<0.05),而归肾丸化裁组和阳性药组能显著降低T和LH水平(P<0.05),归肾丸化裁组和阳性药组还能显著升高FSH水平(P<0.05)。与正常组相比,模型组血清肿瘤坏死因子-α(tumor necrosis factor-alpha,TNF-α)、白细胞介素-6(interleukin-6,IL-6)和白细胞介素-1 β(interleukin-1 beta,IL-1β)水平显著升高(P<0.001)。与模型组相比,归肾丸化裁组和阳性药组均能显著降低PCOS大鼠血清中TNF-α、IL-6和IL-1β水平(P<0.01和P<0.05)。与正常组相比,模型组卵巢组织中IL-6、IL-1β、TNF-α和TLR4 mRNA水平显著升高(P<0.05,P<0.01),归肾丸化裁组与模型组相比IL-6、IL-1β和TLR4 mRNA水平显著下降(P<0.05),TNF-αmRNA水平有降低趋势(P=0.188)。与正常组相比,模型组卵巢组织中TLR4蛋白表达显著升高(P<0.05),NF-κB p65和核因子κB抑制蛋白α(Inhibitor of NF-κB alpha,IκBα)磷酸化水平显著升高(P<0.05),与模型组相比,归肾丸化裁组TLR4蛋白水平显著下降(P<0.05),NF-κB p65和IκBα磷酸化水平显著下降(P<0.05)。

结论

归肾丸化裁可能通过抑制TLR4/NF-κB信号通路的激活,减少炎症因子的产生,进而改善PCOS大鼠的生殖功能障碍和卵巢炎症状态,为临床治疗PCOS提供了新的思路和理论依据。

归肾丸化裁  /  多囊卵巢综合征  /  大鼠模型  /  TLR4/NF-κB信号通路  /  血清激素  /  炎症因子
Objective

To research the therapeutic effects of Modified Guishen Wan (归肾丸化裁) on rat model of polycystic ovary syndrome (PCOS) by regulating Toll-like receptor 4 (TLR4) /nuclear factor-κB (NF-κB) signaling pathway, particularly its impact on ovarian function, serum hormone levels and inflammatory factor expression.

Method

Forty 8-week-old SPF female SD rats with normal estrous cycle were randomly divided into four groups (n=10) : normal group, model group, Modified Guishen Wan group and positive drug group (metformin). Except for the normal group, the other groups were administered letrozole via gavage for 21 consecutive days to establish the PCOS model. The Modified Guishen Wan group and the positive drug group received the corresponding treatments for 21 days. After the experiment, the estrous cycle, ovarian index, serum sex hormone levels, serum inflammatory factor levels, the mRNA levels of inflammation-related genes in ovarian tissue and protein expression were detected to evaluate the efficacy of the Modified Guishen Wan.

Result

The estrous cycle of the normal group was 3-5 days/cycle. After 21 days of modeling, the estrous cycles of the other groups became disordered, with an average of 5-8 days/cycle, mostly in the interphase or prophase, and some lacked a complete estrous cycle, indicating ovarian damage. After 3 weeks of gavage treatment, the estrous cycles of the positive group and the Modified Guishen Wan group recovered. Compared with that of the normal group, the ovarian index of the model group significantly increased (P<0.01). Compared with that of the model group, the ovarian index of the rats of the Modified Guishen Wan group and the positive drug group significantly decreased (P<0.01), approaching the normal group levels. The ovarian tissue of the model group showed multiple cystic changes, with reduced or absent corpora lutea. In contrast, the Modified Guishen Wan group and the positive drug group had fewer cystic and atretic follicles, and the granulosa cell layer structure tended to be normal. The levels of testosterone (T) and luteinizing hormone (LH) in the model group were significantly higher (P<0.01, P<0.05), while follicle-stimulating hormone (FSH) levels were significantly lower (P<0.05). The Modified Guishen Wan group and the positive drug group could significantly reduce the levels of T and LH (P<0.05), and the Modified Guishen Wan group and the positive drug group could also significantly increase the FSH levels (P<0.05). Compared with those of the normal group, the serum levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1 β (IL-1β) in the model group were significantly elevated (P<0.001). Compared with the model group, both the Modified Guishen Wan and the positive drug could significantly reduce the serum levels of TNF-α, IL-6 and IL-1β in PCOS rats (P<0.01 and P<0.05). Compared with those of the normal group, the mRNA levels of IL-6, IL-1β, TNF-α and TLR4 in the ovarian tissue of the model group were significantly increased (P<0.05, P<0.01). The Modified Guishen Wan group had significantly lower levels of IL-6, IL-1β and TLR4 mRNA compared to the model group (P<0.05), with a trend toward lower TNF-αmRNA levels (P=0.188). Compared with the normal group, the protein expression of TLR4 in the ovarian tissue of the model group was significantly increased (P<0.05), and the phosphorylation levels of NF-κB p65 and IκBα were significantly elevated (P<0.05). The Modified Guishen Wan group had significantly lower TLR4 protein levels (P<0.05) and significantly reduced phosphorylation levels of NF-κB p65 and IκBα (P<0.05).

Conclusion

The Modified Guishen Wan may improve reproductive dysfunction and ovarian inflammation in PCOS rats by inhibiting the activation of the TLR4/NF-κB signaling pathway and reducing the production of inflammatory factors, providing new insights and theoretical basis for the clinical treatment of PCOS.

Modified Guishen Wan (归肾丸化裁)  /  polycystic ovary syndrome  /  rat model  /  TLR4/NF-κB signal path  /  serum hormones  /  inflammatory factors
张晓芳, 沈颖, 叶利群. 归肾丸化裁通过调控TLR4/NF-κB信号通路改善PCOS大鼠炎症反应的研究. 中华中医药学刊, 2026 , 44 (6) : 140 -145 . DOI: 10.13193/j.issn.1673-7717.2026.06.025
Xiaofang ZHANG, Ying SHEN, Liqun YE. Effect of Modified Guishen Wan (归肾丸化裁) on Improving Inflammatory Reaction in PCOS Rats by Regulating TLR4/NF-κB Signal Pathway[J]. Chinese Archives of Traditional Chinese Medicine, 2026 , 44 (6) : 140 -145 . DOI: 10.13193/j.issn.1673-7717.2026.06.025
  • 国家中医药管理局科技司-浙江省中医药管理局共建科技计划项目(GZY-ZJ-KJ-23090)
  • 浙江省中医药科技计划项目(2016ZB112)
  • 宁波市科技重点研发计划“揭榜挂帅”项目(2023Z206)
  • 宁波市自然科学基金项目(2023J240)
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2026年第44卷第6期
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doi: 10.13193/j.issn.1673-7717.2026.06.025
  • 首发时间:2026-10-14
  • 出版时间:2026-06-10
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国家中医药管理局科技司-浙江省中医药管理局共建科技计划项目(GZY-ZJ-KJ-23090)
浙江省中医药科技计划项目(2016ZB112)
宁波市科技重点研发计划“揭榜挂帅”项目(2023Z206)
宁波市自然科学基金项目(2023J240)
作者信息
    浙江中医药大学附属宁波市中医院,浙江 宁波 315010

通讯作者:

叶利群(1973-),女,浙江宁波人,主任医师,硕士,研究方向:中医药治疗妇科常见病。E-mail:。
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