中华中医药学刊

黄精黄芩通过NF-κB/IκB-α通路提升非小细胞肺癌对顺铂的敏感性

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张嘉铖 , 张雯 , 范理宏

中华中医药学刊 | 国家项目点击 2026,44(6): 104-109

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中华中医药学刊 |国家项目点击 2026 , 44 (6) : 104 -109

黄精黄芩通过NF-κB/IκB-α通路提升非小细胞肺癌对顺铂的敏感性

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张嘉铖, 张雯, 范理宏

作者信息

上海同济大学医学院附属第十人民医院，上海 200072

通讯作者:

范理宏（1966-），女，上海人，教授，博士研究生导师，博士，研究方向：肺结节发病机制和线粒体能量医学。E-mail：15770638955@163.com。

作者简介:

张嘉铖（1999-），男，江西吉安人，博士在读，研究方向：肺癌及线粒体能量医学。

Anti-Tumor Sensitivity of Cisplatin About NSCLC Enhanced by Huangjing (Polygonati Rhizoma) -Huangqin (Scutellariae Radix) During NF-κB/IκB-αPathway

Jiacheng ZHANG, Wen ZHANG, Lihong FAN

Affiliations

Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai 200072, China

出版时间: 2026-06-10 doi: 10.13193/j.issn.1673-7717.2026.06.018

文章导航

摘要

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目的

探究中药复方黄精加黄芩对顺铂抗肿瘤的增敏作用，并进一步研究其潜在机制。

方法

构建小鼠肺癌皮下瘤模型，分别给予黄精黄芩（HH）、顺铂（cisplatin，DDP）、黄精黄芩联用顺铂处理（HH+DDP），观察小鼠肿瘤体积变化。体外培养小鼠肺癌LLC（Lewis Lung Carcinoma）细胞系，施加相应干预后分别检测细胞增殖活力、细胞凋亡和活性氧（ROS）产生情况，以及线粒体膜电位的改变情况。

结果

体内外结果表明，HH+DDP组相较于DDP组和HH组可以显著抑制LLC细胞系生长增殖。HH+DDP组干预后，凋亡蛋白相关蛋白Bax和Bcl-2的比值升高，Caspase-3表达上调，促进了细胞凋亡，抑制了肿瘤细胞增殖。同时，还发现HH+DDP组对NF-κB/IκB-α有显著抑制作用。流式细胞术发现HH+DDP组出现更高程度的细胞凋亡和ROS产生。此外，HH+DDP组还降低了线粒体膜电位，促进了细胞凋亡发生。

结论

黄精加黄芩联用顺铂可以显著提升其抗肿瘤敏感性，并且通过线粒体相关的细胞凋亡途径诱导细胞凋亡，抑制体内外肿瘤细胞增殖。

关键词

黄精 / 黄芩 / 顺铂敏感性 / 细胞凋亡 / 线粒体

Abstract

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Objective

To investigate the sensitization effect of traditional Chinese medicine compound Huangjing (Polygonati Rhizoma) and Huangqin (Scutellariae Radix) on the anti-tumor effect of cisplatin (DDP), and further investigate its potential mechanism.

Method

A subcutaneous tumor model of lung cancer in mice was constructed, and Huangjing (Polygonati Rhizoma) -Huangqin (Scutellariae Radix) (HH), DDP and HH combined DDP (HH+DDP) were administered to observe the changes in tumor volume in mice. Lewis cell lines were cultured in vitro, and the corresponding interventions were applied to detect the cell proliferation activity, apoptosis, reactive oxygen species (ROS) production, as well as the changes in mitochondrial membrane potential.

Result

In vitro and in vivo results showed that the HH+DDP group significantly inhibited the growth and proliferation of LLC cell lines compared to the DDP group and HH group. After the intervention in the HH+DDP group, the ratio of apoptotic protein related proteins Bax and Bcl-2 increased, and the expression of Caspase-3 was up-regulated, promoting the apoptosis and inhibiting the tumor cell proliferation. At the same time, it was also found that the HH+DDP group had a significant inhibitory effect on nuclear factor-κB (NF-κB) /inhibitor of NF-κB (IκB-α). Flow cytometry revealed higher levels of apoptosis and ROS production in the HH+DDP group. In addition, the HH+DDP group also reduced mitochondrial membrane potential and promoted the apoptosis.

Conclusion

HH combined DDP can significantly enhance its anti-tumor sensitivity and induce apoptosis through mitochondrial related apoptosis pathways, inhibiting tumor cell proliferation in vitro and in vivo.

Key words

Huangjing (Polygonati Rhizoma) / Huangqin (Scutellariae Radix) / cisplatin sensitivity / apoptosis / mitochondria

引用本文

张嘉铖, 张雯, 范理宏. 黄精黄芩通过NF-κB/IκB-α通路提升非小细胞肺癌对顺铂的敏感性. 中华中医药学刊, 2026 , 44 (6) : 104 -109 . DOI: 10.13193/j.issn.1673-7717.2026.06.018

Jiacheng ZHANG, Wen ZHANG, Lihong FAN. Anti-Tumor Sensitivity of Cisplatin About NSCLC Enhanced by Huangjing (Polygonati Rhizoma) -Huangqin (Scutellariae Radix) During NF-κB/IκB-αPathway[J]. Chinese Archives of Traditional Chinese Medicine, 2026 , 44 (6) : 104 -109 . DOI: 10.13193/j.issn.1673-7717.2026.06.018

基金

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国家中医药管理局新型冠状病毒感染中医药应急专项(2023ZYLCYJ02-6)
上海市科委国际合作项目(20400750600)
上海市卫生健康委员会中西医结合创新旗舰医院建设项目(ZY〔2021-2023〕-0205-05; ZXXT-202203)

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2026年第44卷第6期

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doi: 10.13193/j.issn.1673-7717.2026.06.018

首发时间：2026-10-14
出版时间：2026-06-10

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基金

国家中医药管理局新型冠状病毒感染中医药应急专项(2023ZYLCYJ02-6)

上海市科委国际合作项目(20400750600)

上海市卫生健康委员会中西医结合创新旗舰医院建设项目(ZY〔2021-2023〕-0205-05; ZXXT-202203)

作者信息

上海同济大学医学院附属第十人民医院，上海 200072

通讯作者:

范理宏（1966-），女，上海人，教授，博士研究生导师，博士，研究方向：肺结节发病机制和线粒体能量医学。E-mail：15770638955@163.com。

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https://castjournals.cast.org.cn/joweb/zhzyyxk/CN/10.13193/j.issn.1673-7717.2026.06.018

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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