中华中医药学刊

左归丸减轻雷公藤多苷卵巢毒性的蛋白质组学研究

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韩姗姗 ¹ , 任晓丹 ¹^,² , 丁樱 ¹^,² , 任献青 ¹^,² , 张霞 ¹ , 孙昭恒 ¹^,² , 代彦林 ¹

中华中医药学刊 | 国家项目点击 2026,44(6): 99-104

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中华中医药学刊 |国家项目点击 2026 , 44 (6) : 99 -104

左归丸减轻雷公藤多苷卵巢毒性的蛋白质组学研究

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韩姗姗¹, 任晓丹¹^,², 丁樱¹^,², 任献青¹^,², 张霞¹, 孙昭恒¹^,², 代彦林¹

作者信息

^1.河南中医药大学儿科医学院，河南郑州 450000

^2.河南中医药大学第一附属医院儿科医院，河南郑州 450001

通讯作者:

丁樱（1951-），女，江苏南京人，教授、主任医师，博士研究生导师，研究方向：中医药防治小儿风湿免疫及肾脏疾病。E-mail：dingying3236@sina.com。

作者简介:

韩姗姗（1988-），女，河南郑州人，副主任医师，硕士研究生导师，博士，研究方向：中医药防治小儿肾脏疾病。

Proteomic Study of Zuogui Wan (左归丸) for Reducing Ovarian Toxicity of Tripterygium Glycosides

Shanshan HAN¹, Xiaodan REN¹^,², Ying DING¹^,², Xianqing REN¹^,², Xia ZHANG¹, Zhaoheng SUN¹^,², Yanlin DAI¹

Affiliations

^1.College of Pediatrics, Henan University of Chinese Medicine, Zhengzhou 450000, Henan, China

^2.Pediatrics Hospital, The First Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou 450001, Henan, China

出版时间: 2026-06-10 doi: 10.13193/j.issn.1673-7717.2026.06.017

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摘要

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目的

评估左归丸（ZGW）减轻雷公藤多苷（TG）卵巢毒性的作用并利用蛋白质组学方法探讨其减毒机制。

方法

选取32只SPF级2月龄雌性SD大鼠随机分为空白（CON）组（10只）和模型组（22只），对模型组大鼠连续灌胃2周TG（50 mg·kg^-1·d^-1）构建卵巢损伤模型，将造模成功的大鼠（20只）随机分为TG组、左归丸（ZGW）组（1.875 g·kg^-1·d^-1），每组10只，连续灌胃4周，灌胃期间统计大鼠动情周期，灌胃结束后测量大鼠体质量、卵巢及子宫指数，酶联免疫吸附测定法测定血清性激素水平，苏木精-伊红（HE）染色观察卵巢组织病理损伤，Label Free定量蛋白质组学技术进行分析，并通过Western Blot验证富集通路的蛋白表达情况。

结果

ZGW能有效调节TG诱导的雌性大鼠动情周期及性激素水平变化，显著减少闭锁卵泡产生（P<0.000 1）。蛋白质组学表明，与CON组相比，TG组有203个差异蛋白，经过TG治疗后，有28个差异蛋白显著回调，KEGG富集分析涉及鞘糖脂生物合成、糖胺聚糖降解、其他聚糖降解、细胞凋亡等信号通路。Western Blot结果表明，ZGW可以下调细胞凋亡信号通路上的细胞色素C（Cycs）、聚ADP核糖聚合酶家族成员3（Parp3）、细胞凋亡调节因子Bax（Bax）、半胱氨酸蛋白酶-3（Caspase-3）和上调B淋巴细胞瘤-2基因（Bcl-2）蛋白表达。

结论

ZGW可以调节由Cycs、Parp3、Bax、Caspase-3、Bcl-2等介导的细胞凋亡信号通路，发挥减轻TG对卵巢组织损伤的作用。

关键词

左归丸 / 雷公藤多苷片 / 卵巢毒性 / 蛋白质组学

Abstract

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Objective

To evaluate the effect of Zuogui Wan (左归丸, ZGW) on reducing ovarian toxicity of Tripterygium glycosides (TG) and to explore its attenuated mechanism by proteomic methods.

Methods

Thirty-two 2-month-old female SD rats with SPF grade were randomly divided into control (CON) group (10 rats) and model group (22 rats). The model group rats were given continuous intragastatic administration of TG (50 mg·kg^-1·d^-1) for 2 weeks to construct the ovarian injury model. The rats with successful model (20) were randomly divided into TG group and ZGW group (1.875 g·kg^-1·d^-1) with 10 rats in each group. The estrus cycle of the rats was measured during continuous gavage for 4 weeks. After gavage, the body weight, ovarian and uterine indexes were measured, and serum sex hormone levels were determined by enzyme-linked immunosorbent assay (ELISA). Hematoxylin-eosin (HE) staining was used to observe the pathological damage of ovarian tissue, and label free quantitative proteomic analysis was performed. Western Blot was used to verify the protein expression of the enrichment pathway.

Results

ZGW could effectively regulate the estrous cycle and sex hormone level of TG-induced female rats, and significantly reduce the production of atretic follicles (P<0.000 1). Proteomics showed that compared with those in the CON group, there were 203 differential proteins in the TG group, and 28 differential proteins were significantly reduced after TG treatment. KEGG enrichment analysis involved signaling pathways such as sphingolipid biosynthesis, glycosaminoglycan degradation, degradation of other glycans and cell apoptosis. Western Blot results showed that ZGW could down-regulate the expressions of Cycs, Parp3, Bax and Caspase-3 and up-regulate the expression of Bcl-2 protein in apoptosis signaling pathway.

Conclusion

ZGW can regulate the apoptosis signaling pathway mediated by Cycs, Parp3, Bax, Caspase-3 and Bcl-2 and plays a role in alleviating the damage of ovarian tissue caused by TG.

Key words

Zuogui Wan (左归丸) / Tripterygium Glycosides Tablets / ovarian toxicity / proteomics

引用本文

韩姗姗, 任晓丹, 丁樱, 任献青, 张霞, 孙昭恒, 代彦林. 左归丸减轻雷公藤多苷卵巢毒性的蛋白质组学研究. 中华中医药学刊, 2026 , 44 (6) : 99 -104 . DOI: 10.13193/j.issn.1673-7717.2026.06.017

Shanshan HAN, Xiaodan REN, Ying DING, Xianqing REN, Xia ZHANG, Zhaoheng SUN, Yanlin DAI. Proteomic Study of Zuogui Wan (左归丸) for Reducing Ovarian Toxicity of Tripterygium Glycosides[J]. Chinese Archives of Traditional Chinese Medicine, 2026 , 44 (6) : 99 -104 . DOI: 10.13193/j.issn.1673-7717.2026.06.017

基金

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国家自然科学基金项目(82205187)

参考文献

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2026年第44卷第6期

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文章信息

doi: 10.13193/j.issn.1673-7717.2026.06.017

首发时间：2026-10-14
出版时间：2026-06-10

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出版历史

基金

国家自然科学基金项目(82205187)

作者信息

^1.河南中医药大学儿科医学院，河南郑州 450000

^2.河南中医药大学第一附属医院儿科医院，河南郑州 450001

通讯作者:

丁樱（1951-），女，江苏南京人，教授、主任医师，博士研究生导师，研究方向：中医药防治小儿风湿免疫及肾脏疾病。E-mail：dingying3236@sina.com。

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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