中华中医药学刊

基于蛋白组学探讨三七改善血瘀证阿霉素肾纤维化模型大鼠的作用机制

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吴金玉 ¹ , 陆良喜 ² , 黄志敏 ¹

中华中医药学刊 | 博士导师新论 2026,44(5): 7-12

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中华中医药学刊 |博士导师新论 2026 , 44 (5) : 7 -12

基于蛋白组学探讨三七改善血瘀证阿霉素肾纤维化模型大鼠的作用机制

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吴金玉¹, 陆良喜², 黄志敏¹

作者信息

^1．广西中医药大学第一附属医院，广西中医药防治医学分子生物重点实验室，广西南宁 530023

^2．广西中医药大学，广西南宁 530001

通讯作者:

黄志敏（1988-），女，广西贵港人，副主任医师，博士，研究方向：中医药防治风湿免疫疾病、肾脏病。E-mail：547953387@qq.com。

作者简介:

吴金玉（1965-），女，广东广州人，教授、主任医师，博士研究生导师，博士，研究方向：中医药防治风湿免疫疾病、肾脏病。

Mechanism of Sanqi (Notoginseng Radix et Rhizoma) Improving Blood Stasis Syndrome in Rats with Adriamycin-Induced Nephrotoxic Fibrosis Using Proteomics

Jinyu WU¹, Liangxi LU², Zhimin HUANG¹

Affiliations

^1. The First Affiliated Hospital of Guangxi University of Chinese Medicine, Guangxi Key Laboratory of Molecular Biology of Preventive Medicine of Traditional Chinese Medicine, Nanning 530023, Guangxi, China

^2. Guangxi University of Chinese Medicine, Nanning 530001, Guangxi, China

出版时间: 2026-05-10 doi: 10.13193/j.issn.1673-7717.2026.05.002

文章导航

摘要

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目的

基于蛋白组学探讨三七改善血瘀证阿霉素肾纤维化大鼠的作用机制。

方法

将30只SD大鼠随机分为6组：正常组、模型组、三七低剂量组、三七中剂量组、三七高剂量组、阳性对照组，每组5只。采用LC-MS技术同时结合生物信息学分析等对肾组织蛋白进行鉴定、定量分析，筛选差异蛋白，对差异蛋白进行GO和KEGG富集分析。

结果

通过各实验组动物肾组织蛋白组学检测分析，模型组鉴定出与正常组有统计差异的478个蛋白，其中146个表达上调、332个表达下调。三七中剂量组鉴定出与模型组有统计差异的180个蛋白，包括76个表达上调、104个表达下调的蛋白。通过对三七中剂量组、模型组、正常组进行鉴定分析，三七可能调控的蛋白7个：其中三七可能上调的蛋白为Gtf2f2和LOC681410，可能下调的蛋白为RT1-Ba、Cnpy4、Pdcd11、Gimap4、P3h1。KEGG通路富集结果显示最为显著的是能量代谢通路Metabolic pathways。

结论

三七可能通过调节差异肾组织蛋白Gtf2f2、LOC681410、RT1-Ba、Cnpy4、Pdcd11、Gimap4和P3h1的表达，调控相关能量代谢通路Metabolic pathways，从多靶点、多途径改善血瘀证阿霉素大鼠肾纤维化进程。

关键词

慢性肾脏病 / 肾纤维化 / 蛋白组学 / 三七 / 血瘀证

Abstract

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Objective

To investigate the mechanism of Sanqi (Notoginseng Radix et Rhizoma) improving the renal fibrosis induced by adriamycin in rats with blood stasis syndrome based on proteomics.

Methods

Thirty SD rats were randomly divided into 6 groups: normal group, model group, Sanqi (Notoginseng Radix et Rhizoma) low-dose group, Sanqi (Notoginseng Radix et Rhizoma) medium-dose group, Sanqi (Notoginseng Radix et Rhizoma) high-dose group and positive control group, with 5 rats in each group. Using LC-MS technology in combination with bioinformatics analysis, protein identification, quantification and differential protein screening were performed on renal tissues. Differential proteins were subjected to GO and KEGG enrichment analysis.

Results

There were 478 proteins in the model group compared with those in the normal group, of which 146 proteins were up-regulated and 332 proteins were down-regulated. Compared with those in the model group, there were 180 different proteins in the Sanqi (Notoginseng Radix et Rhizoma) medium-dose group, including 76 up-regulated and 104 down-regulated proteins. Combined with the above experimental results, further analysis of the Sanqi (Notoginseng Radix et Rhizoma) medium-dose group, the model group and the normal group showed that there were 7 differentially expressed proteins regulated by Sanqi (Notoginseng Radix et Rhizoma) . Among them, the proteins that Sanqi (Notoginseng Radix et Rhizoma) may up-regulate were Gtf2f2 and LOC681410. The possible down-regulated proteins were RT1-Ba, Cnpy4, Pdcd11, Gimap4 and P3h1. KEGG pathway enrichment results showed that the most significant energy pathway was metabolic pathways.

Conclusion

Sanqi (Notoginseng Radix et Rhizoma) may play a role in anti-renal fibrosis in adriamycin-induced rat model with blood stasis syndrome through multi-target and multi-pathway by regulating differential expressions of renal tissue proteins Gtf2f2, LOC681410, RT1-Ba, Cnpy4, Pdcd11, Gimap4 and P3h1 and modulating metabolic pathways associated with energy metabolism.

Key words

chronic kidney disease / renal fibrosis / proteomics / Sanqi (Notoginseng Radix et Rhizoma) / blood stasis syndrome

引用本文

吴金玉, 陆良喜, 黄志敏. 基于蛋白组学探讨三七改善血瘀证阿霉素肾纤维化模型大鼠的作用机制. 中华中医药学刊, 2026 , 44 (5) : 7 -12 . DOI: 10.13193/j.issn.1673-7717.2026.05.002

Jinyu WU, Liangxi LU, Zhimin HUANG. Mechanism of Sanqi (Notoginseng Radix et Rhizoma) Improving Blood Stasis Syndrome in Rats with Adriamycin-Induced Nephrotoxic Fibrosis Using Proteomics[J]. Chinese Archives of Traditional Chinese Medicine, 2026 , 44 (5) : 7 -12 . DOI: 10.13193/j.issn.1673-7717.2026.05.002

基金

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国家自然科学基金项目(81960866; 82160878)
中医学广西一流学科项目(桂教科研〔2022〕1号)
广西中医药大学引进博士科研启动基金项目(2023BS040)

参考文献

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2026年第44卷第5期

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文章信息

doi: 10.13193/j.issn.1673-7717.2026.05.002

首发时间：2026-10-14
出版时间：2026-05-10

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基金

国家自然科学基金项目(81960866; 82160878)

中医学广西一流学科项目(桂教科研〔2022〕1号)

广西中医药大学引进博士科研启动基金项目(2023BS040)

作者信息

^1．广西中医药大学第一附属医院，广西中医药防治医学分子生物重点实验室，广西南宁 530023

^2．广西中医药大学，广西南宁 530001

通讯作者:

黄志敏（1988-），女，广西贵港人，副主任医师，博士，研究方向：中医药防治风湿免疫疾病、肾脏病。E-mail：547953387@qq.com。

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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