中华中医药学刊

三七总皂苷对胃癌前病变复合心肌缺血再灌注损伤小鼠的保护作用及机制研究

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王晓燕 , 李浩

中华中医药学刊 | 国家项目点击 2026,44(3): 34-38

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中华中医药学刊 |国家项目点击 2026 , 44 (3) : 34 -38

三七总皂苷对胃癌前病变复合心肌缺血再灌注损伤小鼠的保护作用及机制研究

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王晓燕, 李浩

作者信息

中国中医科学院望京医院，北京 100102

通讯作者:

李浩（1965 -），男，河南开封人，主任医师，博士研究生导师，博士，研究方向：老年心脑血管疾病。E-mail：wjhplihao1965@126.com。

作者简介:

王晓燕（1986 -），女，山东济南人，主任医师，硕士研究生导师，博士，研究方向：中西医结合肿瘤及心血管疾病。

Protective Effect and Mechanism of Sanqi（Panax notoginseng）Saponins on Gastric Precancerous Lesions Combined with Myocardial Ischemia-Reperfusion Injury in Mice

Xiaoyan WANG, Hao LI

Affiliations

Wangjing Hospital, China Academy of Chinese Medical Sciences, Beijing 100102, China

出版时间: 2026-03-10 doi: 10.13193/j.issn.1673-7717.2026.03.006

文章导航

摘要

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目的

探讨三七总皂苷对胃癌前病变复合心肌缺血再灌注损伤小鼠胃黏膜的保护机制。

方法

85只C57BL/6小鼠分为6组，分别为空白对照组（C组），模型组（M组），三七总皂苷高、中、低剂量组（SH、SM、SL组）和阳性对照组（R组）。C组10只小鼠不造模，其余每组15只小鼠均造模。化学诱导法+隔日禁食310 d建立胃癌前病变小鼠模型，于第311 d对造模组小鼠进行心肌缺血再灌注损伤手术，建立胃癌前病变复合心肌缺血再灌注损伤小鼠模型。SH、SM和SL组小鼠分别给予300、150、75 mg/kg的三七总皂苷，R组给予1.5 mg/kg雷帕霉素，C组和M组给予等量的蒸馏水。胃癌前病变造模期间灌胃给药1次/d，心肌缺血再灌注损伤造模结束后继续给药1周。使用苏木精伊红染色（Hematoxylin-eosin Staining，HE）和阿利新蓝-过碘酸-雪夫染色（Alcian blue-Periodic acid-Schiffreagent，AB-PAS）染色观察小鼠胃黏膜病理改变，2，3，5-氯化三苯基四氮唑（TTC）染色观察心脏梗死区域并称重，使用蛋白质免疫印迹（Western blot，WB）分析自噬相关蛋白的表达。

结果

组织病理分析显示M组小鼠胃组织内固有层排列松散，明显可见细胞萎缩空洞出现及微量炎症浸润，局部胞核坏死消融，上皮细胞处紫红色颗粒量明显减少，有明显细胞脱落，而SH和R组小鼠胃组织病理明显改善。TTC染色和组织称重结果显示，与M组比较，SH组、SM组、SL组和R组小鼠心脏梗死和缺血区域及重量明显降低（P < 0.001）。WB结果显示，与M组比较，SH组、SM组和R组小鼠胃组织LC3 Ⅱ/LC3 Ⅰ、Beclin-1和ATG5蛋白表达上调（P < 0.05或P < 0.01），而p62、AKT、p-AKT、mTOR和p-mTOR蛋白表达下调（P < 0.05或P < 0.01）。

结论

三七总皂苷可通过抑制PI3K/AKT/mTOR信号通路激活自噬来预防小鼠胃癌前病变和缓解小鼠心肌缺血再灌注损伤。

关键词

胃癌前病变 / 心肌缺血再灌注 / 三七总皂苷

Abstract

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Objective

To investigate the protective mechanism of Sanqi（Panax notoginseng）saponins on gastric mucosa in mice with gastric precancerous lesions combined with myocardial ischemia-reperfusion injury.

Methods

Eighty-five C57BL/6 mice were divided into 6 groups：blank control group（C group）, model group（M group）, high, medium and low dose groups of Sanqi（Panax notoginseng）saponins（SH, SM, SL groups）, and positive control group（R group）. Except for group C which had 10 animals without modeling, all the other groups had 15 animals in each. A mouse model of gastric precancerous lesions was established by chemical induction and fasting every other day for 310 days. On the 311th day, the model mice underwent the myocardial ischemia-reperfusion injury surgery to establish a mouse model of gastric precancerous lesions combined with myocardial ischemia-reperfusion injury. SH, SM and SL groups were given Sanqi（Panax notoginseng）saponins with the dose of 300, 150, 75 mg/kg, respectively. R group was given rapamycin（1.5 mg/kg）, and C and M groups were given the equal amounts of distilled water. During the modeling of gastric precancerous lesions, the drug was given once a day by intragastric administration. After the completion of myocardial ischemia-reperfusion injury modeling, the drug was continued to administer once a day for 1 week. HE and AB-PAS staining were used to observe the pathological changes of gastric mucosa. TTC staining was used to observe the heart infarction area and weigh it, and Western blot was used to analyze the expressions of autophagy related proteins.

Results

Histopathological analysis showed that the intrinsic layer of the gastric tissue of mice in group M was arranged in a loose manner, the cell atrophy cavity and trace inflammatory infiltration were evident, the local nucleus necrosis was ablated, the amount of purplish red particles in the epithelial cells was significantly reduced, and there was obvious cell shedding, while the gastric histopathology of mice in SH and R groups was significantly improved. The TTC staining and tissue weighing results showed that compared with the M group, the SH group, SM group, SL group and R group had significantly reduced heart infarction and ischemic area and weight（P < 0.001）. The Western blot results showed that compared with those of the M group, the expressions of LC3 Ⅱ/LC3 Ⅰ, Beclin-1 and ATG5 proteins in the gastric tissues of SH group, SM group and R group mice were up-regulated（P < 0.05 or P < 0.01）, while the expressions of p62, AKT, p-AKT, mTOR and p-mTOR proteins were down-regulated（P < 0.05 or P < 0.01）.

Conclusion

Sanqi（Panax notoginseng）saponins can prevent precancerous lesions and alleviate myocardial ischemia-reperfusion injury in mice by inhibiting the PI3K/AKT/mTOR signaling pathway and activating autophagy.

Key words

precancerous lesions of gastric cancer / myocardial ischemia-reperfusion / Sanqi（Panax notoginseng）saponins

引用本文

王晓燕, 李浩. 三七总皂苷对胃癌前病变复合心肌缺血再灌注损伤小鼠的保护作用及机制研究. 中华中医药学刊, 2026 , 44 (3) : 34 -38 . DOI: 10.13193/j.issn.1673-7717.2026.03.006

Xiaoyan WANG, Hao LI. Protective Effect and Mechanism of Sanqi（Panax notoginseng）Saponins on Gastric Precancerous Lesions Combined with Myocardial Ischemia-Reperfusion Injury in Mice[J]. Chinese Archives of Traditional Chinese Medicine, 2026 , 44 (3) : 34 -38 . DOI: 10.13193/j.issn.1673-7717.2026.03.006

基金

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国家自然科学基金项目(82360962; 82004287)
中央级公益性科研院所基本科研业务费专项(ZZ14-YQ-019)
中国中医科学院科技创新工程重大攻关项目(CI2021A01813)
西藏自治区自然科学基金重点项目(XZ202401ZR0074)

参考文献

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文献

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参考文献引证文献

排序方式：

［1］

CHOI Y Y, AN J Y, KIM H I, et al. Current practice of gastric cancer treatment［J］.中华医学杂志（英文版）, 2014, 127（3）：547-553.

［2］

张泰，胡蓝烁，刘炯，等.胃癌前病变动物模型研究现状及其评价［J］.中国中西医结合消化杂志，2024，32（5）：412-419.

［3］

THRIFT A P, EL-SERAG H B. Burden of gastric cancer［J］. Clinical Gastroenterology and Hepatology, 2020, 18（3）：534-542.

［4］

张晴晴，吴迪，王奕晨，等.中医药治疗胃癌前病变近20年疗效评价指标分析［J］.天津中医药大学学报，2024，43（4）：325-332.

［5］

王萍，史彬，温艳东，等.胃癌前病变病证结合风险预测模型的构建研究［J］.中国中西医结合杂志，2018，38（7）：773-778.

［6］

杜姚，李兆栋，赵宝玉，等.胃癌和胃癌前病变的风险预测研究概况［J］.河北中医，2021，43（8）：1392-1396.

［7］

袁虎方，李勇，汪雁博，等.大株红景天对胃癌合并冠心病患者围术期的心脏保护作用［J］.中国普通外科杂志，2019，28（4）：456-460.

［8］

徐思中，刘嘉琪，李韵然，等.近10年中医辨治胃癌前病变的研究进展［J］.广州中医药大学学报，2024，41（8）：2219-2224.

［9］

逯文敏，王曦，王向涛.三七总皂苷生物利用度提升制剂新技术研究进展［J］.天津中医药，2024，41（5）：672-680.

［10］

蔡甜甜，林琳，潘华峰，等.三七总皂苷激活JNK信号通路对胃癌前病变大鼠胃黏膜组织的保护作用［J］.中华中医药杂志，2019，34（12）：5877-5880.

［11］

路丽娟，陈晓丹，周雪菲.三七总皂苷对冠心病心绞痛患者血浆内皮素-1和血小板功能的影响价值［J］.内蒙古中医药，2024，43（3）：65-66.

［12］

YAN D, LIAN D, CHEN H W, et al. Network pharmacology-based prediction and verification of the targets and mechanism for panax notoginseng saponins against coronary heart disease［J］. Evidence-Based Complementary and Alternative Medicine, 2019, 2019：6503752.

［13］

XU L, XIAO S N, YUAN W H, et al. Synthesis and anticancer activity evaluation of hydrolyzed derivatives of panaxnotoginseng saponins［J］. Molecules, 2018, 23（11）：3021.

［14］

WANG W T, YANG L, SONG L, et al. Combination of Panax notoginseng saponins and aspirin potentiates platelet inhibition with alleviated gastric injury via modulating arachidonic acid metabolism［J］. Biomedicine & Pharmacotherapy, 2021（1）：134.

［15］

ZHANG M F, ZHONG J L, SONG Z Y, et al. Regulatory mechanisms and potential therapeutic targets in precancerous lesions of gastric cancer：A comprehensive review［J］. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2024, 177：117068.

［16］

ZHANG J X, BAO S C, CHEN J, et al. Xiaojianzhong decoction prevents gastric precancerous lesions in rats by inhibiting autophagy and glycolysis in gastric mucosal cells［J］. World journal of gastrointestinal oncology, 2023, 15（3）：464-489.

［17］

ZHANG J X, WANG W B, YAN S G, et al. CagA and VacA inhibit gastric mucosal epithelial cell autophagy and promote the progression of gastric precancerous lesions［J］. Zhong nan da xue xue bao, 2022, 47（7）：942-951.

［18］

QIN G W, LU P, PENG L, et al. Ginsenoside rb1 inhibits cardiomyocyte autophagy via pi3k/akt/mtor signaling pathway and reduces myocardial ischemia/reperfusion injury［J］. The American journal of Chinese medicine, 2021, 49（8）：11-15.

［19］

XU H, CHENG J, HE F. Cordycepin alleviates myocardial ischemia/reperfusion injury by enhancing autophagy via AMPK-mTOR pathway［J］. Journal of Physiology and Biochemistry, 2022, 78（2）：1-13.

［20］

ZHANG J, HU S Y, GAO Y, et al. Galangin alleviated myocardial ischemia-reperfusion injury by enhancing autophagic flux and inhibiting inflammation［J］. European journal of pharmacology, 2023, 945：175621.

［21］

MA S, WANG Y B, CHEN Y D, et al. The role of the autophagy in myocardial ischemia/reperfusion injury［J］. BBA-Molecular Basis of Disease, 2015, 1852（2）：271-276.

［22］

陶爱琳，乔新月，冯晓玲.中药单体调控PI3K/AKT/mTOR通路介导自噬干预妇科常见恶性肿瘤的研究进展［J］.中药药理与临床，2025，41（2）：121-128.

［23］

CAI T T, ZHANG C Z, ZHAO Z M, et al. The gastric mucosal protective effects of astragaloside IV in mnng-induced GPL rats［J］. Biomedicine & Pharmacotherapy, 2018, 104：291-299.

［24］

孙春斌，应艺，侯迥，等.三七总皂苷抑制PI3K/AKT/mTOR信号通路激活自噬缓解小鼠肺纤维化的实验研究［J］.时珍国医国药，2020，31（12）：2872-2876.

［25］

王华，谭超，王子焱，等.三七总皂苷基于PI3K/Akt/mTOR通路调控自噬和泛素蛋白积累对大鼠肾缺血再灌注损伤的影响［J］.现代中西医结合杂志，2023，32（11）：1461-1467，1472.

2026年第44卷第3期

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doi: 10.13193/j.issn.1673-7717.2026.03.006

首发时间：2026-10-14
出版时间：2026-03-10

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出版历史

基金

国家自然科学基金项目(82360962; 82004287)

中央级公益性科研院所基本科研业务费专项(ZZ14-YQ-019)

中国中医科学院科技创新工程重大攻关项目(CI2021A01813)

西藏自治区自然科学基金重点项目(XZ202401ZR0074)

作者信息

中国中医科学院望京医院，北京 100102

通讯作者:

李浩（1965 -），男，河南开封人，主任医师，博士研究生导师，博士，研究方向：老年心脑血管疾病。E-mail：wjhplihao1965@126.com。

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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