Article(id=1317125402397622704, tenantId=1146029695717560320, journalId=1255847919539208197, issueId=1317125400581485316, articleNumber=null, orderNo=null, doi=10.13193/j.issn.1673-7717.2026.03.004, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=null, receivedDateStr=null, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1791956766938, onlineDateStr=2026-10-14, pubDate=1773072000000, pubDateStr=2026-03-10, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1791956766938, onlineIssueDateStr=2026-10-14, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1791956766938, creator=13701087609, updateTime=1791956766938, updator=13701087609, issue=Issue{id=1317125400581485316, tenantId=1146029695717560320, journalId=1255847919539208197, year='2026', volume='44', issue='3', pageStart='1', pageEnd='258', issueExtLink='null', onlineDate='null', pubDate='1773072000000', pubDateStr='2026-03-10', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1791956766504, creator='13701087609', updateTime=1791958763799, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1317133777898418844, tenantId=1146029695717560320, journalId=1255847919539208197, issueId=1317125400581485316, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1317133777898418845, tenantId=1146029695717560320, journalId=1255847919539208197, issueId=1317125400581485316, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=20, endPage=25, ext={EN=ArticleExt(id=1317125402577977777, articleId=1317125402397622704, tenantId=1146029695717560320, journalId=1255847919539208197, language=EN, title=Cloning,Identification and Bioinformatics Analysis of Niubang(Arctium lappa)4CL Gene Families, columnId=1256183361521967297, journalTitle=Chinese Archives of Traditional Chinese Medicine, columnName=Target on National Project, runingTitle=null, highlight=null, articleAbstract=
Objective

4-coumarate CoA ligase(4CL)is a key enzyme in the phenylpropane pathway controlling the biosynthesis of lignan, lignin and other compounds closely related to Niubang(Arctium lappa)microscopic characterization of stone cell development and Niubang(Arctium lappa)glycoside accumulation, the aim of this study was to identify the 4CL genes in Niubang(Arctium lappa)and explore the biological functions of Niubang(Arctium lappa)4CL enzyme gene family's biological functions.

Methods

In this study, it cloned and sequenced 8 predicted 4CL gene coding sequences(CDS)in the burdock genome, performed bioinformatics analysis on the obtained sequences and performed prokaryotic expression of Al4CL3.

Results

The eight members of Niubang(Arctium lappa)4CL gene family were identified and cloned with 100% consistency with the predicted sequences, except for Al4CL5(96%)and Al4CL8, which had five variant sites. Al4CL3, Al4CL5 and Al4CL8 proteins had two transmembrane helical structures; subcellular localization in the cytoplasm, plasma membrane, chloroplast and endoplasmic reticulum and gene structure. There were some differences in the gene structure, unevenly distributed on chromosomes 1, 2, 3, 9, 10 and 14, and there was a 4CL amino acid conserved domain in the amino acid sequence. There were multiple cis-acting elements in the promoter region of the Al4CL gene, which was induced by multiple hormones or stresses. The phylogenetic analysis showed that Al4CL was divided into three subfamilies, and the covariance analysis showed that there was a closer relationship between members of the Al4CL family of genes and the prickly bracteate thistle. There was a closer relationship between members of the Al4CL gene family and the prickly bracteate thistle. Recombinant plasmid pET-28a-chAl4CL3 was constructed, successfully induced the expression of chAl4CL3 protein and purified.

Conclusion

The excavation and characterization of burdock 4CL gene family provides a certain theoretical basis for the biosynthesis of Niubang(Arctium lappa)and its quality improvement, and provides a reference for the in-depth study of Al4CL and its biological functions.

, authors=Yanping XING, Chunyan LI, Xinghua AN, Mingze GAO, Rong ZHAO, Tingguo KANG, Deqiang DOU, Liang XU, authorsList=Yanping XING, Chunyan LI, Xinghua AN, Mingze GAO, Rong ZHAO, Tingguo KANG, Deqiang DOU, Liang XU, authorCompany=null, correspAuthors=Liang XU, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1317125402947076530, articleId=1317125402397622704, tenantId=1146029695717560320, journalId=1255847919539208197, language=CN, title=牛蒡4CL基因家族的克隆鉴定与生物信息学分析, columnId=1256183362172142382, journalTitle=中华中医药学刊, columnName=国家项目点击, runingTitle=null, highlight=null, articleAbstract=
目的

4-香豆酸:辅酶A连接酶(4-coumarate CoA ligase,4CL)是苯丙烷途径中控制木质素、木脂素等化合物生物合成的关键酶与牛蒡子显微特征石细胞发育和牛蒡苷积累密切相关,研究旨在鉴定牛蒡中的4CL基因,探索牛蒡Arctium lappa L.4CL酶基因家族的生物学功能。

方法

研究对牛蒡基因组中预测的8个4CL基因编码序列(Coding sequence,CDS)进行克隆测序,对获得序列进行生物信息学分析,并对Al4CL3进行原核表达。

结果

鉴定得到牛蒡4CL基因家族的8个成员克隆后除Al4CL5(96%)、Al4CL8存在5个变异位点之外,其他序列与预测序列一致性均为100%;预测Al4CL3、Al4CL5、Al4CL8蛋白具有2个跨膜螺旋结构;亚细胞定位于细胞质、质膜、叶绿体和内质网;基因序列结构存在一定差异,不均匀地分布于牛蒡1、2、3、9、10和14号染色体上;Al4CL1、Al4CL6、Al4CL8氨基酸序列中存在4CL氨基酸保守域;Al4CL基因启动子区域存在多个顺式作用元件,受多种激素或胁迫诱导;系统进化分析显示Al4CL分为3个亚族,共线性分析表明Al4CL基因家族成员与刺苞菜蓟之间存在更近的亲缘关系;构建重组质粒pET-28a-chAl4CL3,成功诱导表达出chAl4CL3蛋白,并完成纯化。

结论

对牛蒡4CL基因家族的挖掘与鉴定,为牛蒡木脂素的生物合成及其品质的提高提供了一定的理论依据,为深入研究Al4CL及其生物学功能提供参考。

, authors=邢艳萍, 李春妍, 安星华, 高洺泽, 赵容, 康廷国, 窦德强, 许亮, authorsList=邢艳萍, 李春妍, 安星华, 高洺泽, 赵容, 康廷国, 窦德强, 许亮, authorCompany=null, correspAuthors=许亮, authorNote=

邢艳萍(1986 -),女,辽宁北票人,高级实验师,博士,研究方向:中药资源评价与鉴定。

, correspAuthorsNote=
许亮(1978 -),男,辽宁沈阳人,教授,博士研究生导师,研究方向:中药资源评价鉴定与分子生药。E-mail:。
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邢艳萍(1986 -),女,辽宁北票人,高级实验师,博士,研究方向:中药资源评价与鉴定。

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邢艳萍(1986 -),女,辽宁北票人,高级实验师,博士,研究方向:中药资源评价与鉴定。

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牛蒡4CL基因家族的克隆鉴定与生物信息学分析
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邢艳萍 , 李春妍 , 安星华 , 高洺泽 , 赵容 , 康廷国 , 窦德强 , 许亮
中华中医药学刊 | 国家项目点击 2026,44(3): 20-25
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中华中医药学刊 |国家项目点击 2026 , 44 (3) : 20 -25
牛蒡4CL基因家族的克隆鉴定与生物信息学分析
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邢艳萍, 李春妍, 安星华, 高洺泽, 赵容, 康廷国, 窦德强, 许亮
作者信息
  • 辽宁中医药大学药学院,辽宁 大连 116600
通讯作者:
许亮(1978 -),男,辽宁沈阳人,教授,博士研究生导师,研究方向:中药资源评价鉴定与分子生药。E-mail:。
作者简介:

邢艳萍(1986 -),女,辽宁北票人,高级实验师,博士,研究方向:中药资源评价与鉴定。

Cloning,Identification and Bioinformatics Analysis of Niubang(Arctium lappa)4CL Gene Families
Yanping XING, Chunyan LI, Xinghua AN, Mingze GAO, Rong ZHAO, Tingguo KANG, Deqiang DOU, Liang XU
Affiliations
  • College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, Liaoning, China
出版时间: 2026-03-10 doi: 10.13193/j.issn.1673-7717.2026.03.004
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目的

4-香豆酸:辅酶A连接酶(4-coumarate CoA ligase,4CL)是苯丙烷途径中控制木质素、木脂素等化合物生物合成的关键酶与牛蒡子显微特征石细胞发育和牛蒡苷积累密切相关,研究旨在鉴定牛蒡中的4CL基因,探索牛蒡Arctium lappa L.4CL酶基因家族的生物学功能。

方法

研究对牛蒡基因组中预测的8个4CL基因编码序列(Coding sequence,CDS)进行克隆测序,对获得序列进行生物信息学分析,并对Al4CL3进行原核表达。

结果

鉴定得到牛蒡4CL基因家族的8个成员克隆后除Al4CL5(96%)、Al4CL8存在5个变异位点之外,其他序列与预测序列一致性均为100%;预测Al4CL3、Al4CL5、Al4CL8蛋白具有2个跨膜螺旋结构;亚细胞定位于细胞质、质膜、叶绿体和内质网;基因序列结构存在一定差异,不均匀地分布于牛蒡1、2、3、9、10和14号染色体上;Al4CL1、Al4CL6、Al4CL8氨基酸序列中存在4CL氨基酸保守域;Al4CL基因启动子区域存在多个顺式作用元件,受多种激素或胁迫诱导;系统进化分析显示Al4CL分为3个亚族,共线性分析表明Al4CL基因家族成员与刺苞菜蓟之间存在更近的亲缘关系;构建重组质粒pET-28a-chAl4CL3,成功诱导表达出chAl4CL3蛋白,并完成纯化。

结论

对牛蒡4CL基因家族的挖掘与鉴定,为牛蒡木脂素的生物合成及其品质的提高提供了一定的理论依据,为深入研究Al4CL及其生物学功能提供参考。

4-香豆酸  /  辅酶A连接酶  /  基因克隆  /  生物信息学分析  /  原核表达
Objective

4-coumarate CoA ligase(4CL)is a key enzyme in the phenylpropane pathway controlling the biosynthesis of lignan, lignin and other compounds closely related to Niubang(Arctium lappa)microscopic characterization of stone cell development and Niubang(Arctium lappa)glycoside accumulation, the aim of this study was to identify the 4CL genes in Niubang(Arctium lappa)and explore the biological functions of Niubang(Arctium lappa)4CL enzyme gene family's biological functions.

Methods

In this study, it cloned and sequenced 8 predicted 4CL gene coding sequences(CDS)in the burdock genome, performed bioinformatics analysis on the obtained sequences and performed prokaryotic expression of Al4CL3.

Results

The eight members of Niubang(Arctium lappa)4CL gene family were identified and cloned with 100% consistency with the predicted sequences, except for Al4CL5(96%)and Al4CL8, which had five variant sites. Al4CL3, Al4CL5 and Al4CL8 proteins had two transmembrane helical structures; subcellular localization in the cytoplasm, plasma membrane, chloroplast and endoplasmic reticulum and gene structure. There were some differences in the gene structure, unevenly distributed on chromosomes 1, 2, 3, 9, 10 and 14, and there was a 4CL amino acid conserved domain in the amino acid sequence. There were multiple cis-acting elements in the promoter region of the Al4CL gene, which was induced by multiple hormones or stresses. The phylogenetic analysis showed that Al4CL was divided into three subfamilies, and the covariance analysis showed that there was a closer relationship between members of the Al4CL family of genes and the prickly bracteate thistle. There was a closer relationship between members of the Al4CL gene family and the prickly bracteate thistle. Recombinant plasmid pET-28a-chAl4CL3 was constructed, successfully induced the expression of chAl4CL3 protein and purified.

Conclusion

The excavation and characterization of burdock 4CL gene family provides a certain theoretical basis for the biosynthesis of Niubang(Arctium lappa)and its quality improvement, and provides a reference for the in-depth study of Al4CL and its biological functions.

4-coumarate  /  CoA ligase  /  gene cloning  /  bioinformatics analysis  /  prokaryotic expression
邢艳萍, 李春妍, 安星华, 高洺泽, 赵容, 康廷国, 窦德强, 许亮. 牛蒡4CL基因家族的克隆鉴定与生物信息学分析. 中华中医药学刊, 2026 , 44 (3) : 20 -25 . DOI: 10.13193/j.issn.1673-7717.2026.03.004
Yanping XING, Chunyan LI, Xinghua AN, Mingze GAO, Rong ZHAO, Tingguo KANG, Deqiang DOU, Liang XU. Cloning,Identification and Bioinformatics Analysis of Niubang(Arctium lappa)4CL Gene Families[J]. Chinese Archives of Traditional Chinese Medicine, 2026 , 44 (3) : 20 -25 . DOI: 10.13193/j.issn.1673-7717.2026.03.004
  • 国家自然科学基金面上项目(81773852; 81874338)
  • 辽宁省联合基金项目(2023-MSLH-180)
  • 辽宁省教育厅高校基本科研项目储备项目(2024-JYTCB-022)
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文章信息
doi: 10.13193/j.issn.1673-7717.2026.03.004
  • 首发时间:2026-10-14
  • 出版时间:2026-03-10
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基金
国家自然科学基金面上项目(81773852; 81874338)
辽宁省联合基金项目(2023-MSLH-180)
辽宁省教育厅高校基本科研项目储备项目(2024-JYTCB-022)
作者信息
    辽宁中医药大学药学院,辽宁 大连 116600

通讯作者:

许亮(1978 -),男,辽宁沈阳人,教授,博士研究生导师,研究方向:中药资源评价鉴定与分子生药。E-mail:。
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https://castjournals.cast.org.cn/joweb/zhzyyxk/CN/10.13193/j.issn.1673-7717.2026.03.004
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2种不同金属材料的力学参数
科
Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)
属
Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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