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Article(id=1322923012546232908, tenantId=1146029695717560320, journalId=1255847919539208197, issueId=1322923011518628427, articleNumber=null, orderNo=null, doi=10.13193/j.issn.1673-7717.2025.03.002, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=null, receivedDateStr=null, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1793339024912, onlineDateStr=2026-10-30, pubDate=1741536000000, pubDateStr=2025-03-10, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1793339024912, onlineIssueDateStr=2026-10-30, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1793339024912, creator=13701087609, updateTime=1793339024912, updator=13701087609, issue=Issue{id=1322923011518628427, tenantId=1146029695717560320, journalId=1255847919539208197, year='2025', volume='43', issue='3', pageStart='1', pageEnd='258', issueExtLink='null', onlineDate='null', pubDate='1741536000000', pubDateStr='2025-03-10', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=0, createTime=1793339024666, creator='13701087609', updateTime=1793340602374, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1322929628976906432, tenantId=1146029695717560320, journalId=1255847919539208197, issueId=1322923011518628427, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1322929628981100737, tenantId=1146029695717560320, journalId=1255847919539208197, issueId=1322923011518628427, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=6, endPage=12, ext={EN=ArticleExt(id=1322923012739170893, articleId=1322923012546232908, tenantId=1146029695717560320, journalId=1255847919539208197, language=EN, title=Study on Fermentation Technology of Shenling Baizhu Powder（参苓白术散）and Its Intervention Effect on Alcohol Injury of GES-1 Cells, columnId=1256183423710970242, journalTitle=Chinese Archives of Traditional Chinese Medicine, columnName=New Opinion of Ph.D. Supervisor, runingTitle=null, highlight=null, articleAbstract=
Objective

To study the optimal fermentation conditions of Enterobacter albieri and Cordyceps pupae for Shenling Baizhu Powder（参苓白术散），and evaluate the safety and efficacy of the fermentation solution on alcoholic gastric injury at cell level.

Methods

In this experiment，the contents of functional components in fermentation broth of the modified formula of Shenling Baizhu Powder after fermentation under different conditions were measured and comprehensively evaluated. The alcoholic gastric injury model of GES-1 cells was established after 24 h of alcohol intervention. The intervention effects of water extract（0.938 mg/g）and fermentation broth of the modified formula of Shenling Baizhu Powder at low，medium and high doses（0.469 mg/g，0.938 mg/g，1.876 mg/g）on basic apoptosis of GES-1 cells were investigated.

Results

The optimal fermentation process of Enterobacter asburiae for Shenling Baizhu Powder was pH value 5，inoculation amount 1% and fermentation time1 day. The optimal fermentation process of Shenling Baizhu Powder was pH 6，inoculation amount 3% and fermentation time 3 days. The best inoculation sequence for multistrain fermentation was to vaccinate Enterobacter asburiae first. The contents of ginsenoside Re，hesperidin，total flavonoids and total polysaccharides were significantly increased under the optimal fermentation process. The fermentation solution of Shenling Baizhu Powder improved GES-1 cells at 48 h，and the fermentation solution of medium and high dose groups could reduce the total apoptosis rate of GES-1 cells.

Conclusion

After fermentation，the composition of the modified formula of Shenling Baizhu Powder had a great influence，the content changed obviously and the fermentation process was feasible. It had a proliferation effect on GES-1 cells，and at the same time it could reduce the overall apoptosis rate of GES-1 cells.

, authors=Hongning LIU, Kaiqi ZHENG, Lihua CHEN, Jiaen JIANG, Quan ZHOU, authorsList=Hongning LIU, Kaiqi ZHENG, Lihua CHEN, Jiaen JIANG, Quan ZHOU, authorCompany=null, correspAuthors=Lihua CHEN, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1322923016753119848, articleId=1322923012546232908, tenantId=1146029695717560320, journalId=1255847919539208197, language=CN, title=参苓白术散改良方发酵工艺及对GES-1细胞酒精性损伤的干预作用研究, columnId=1256183426563096985, journalTitle=中华中医药学刊, columnName=博士导师新论, runingTitle=null, highlight=null, articleAbstract=
目的

研究阿氏肠杆菌和蛹虫草真菌发酵参苓白术散改良方最佳发酵条件，并在细胞层面评价发酵液的安全性和对酒精性胃损伤治疗的有效性。

方法

实验检测经不同条件发酵后的参苓白术散改良方发酵液中功能成分的含量，并进行综合评价，在此基础上利用单因素实验对发酵条件进行优化；以酒精作用24 h建立GES-1细胞酒精性损伤模型，考察参苓白术散改良方水提液（0.938 mg/g）和发酵液在低、中、高剂量（0.469、0.938、1.876 mg/g）对人胃黏膜上皮细胞株GES-1细胞酒精性损伤基础凋亡的干预作用。

结果

阿氏肠杆菌发酵参苓白术散改良方的最佳发酵工艺为pH值5，接种量1%，发酵时间1 d。蛹虫草真菌发酵参苓白术散改良方的最佳发酵工艺为pH值6，接种量3%，发酵时间3 d。多菌种发酵最佳接种顺序为先接种阿氏肠杆菌。最佳发酵工艺下人参皂苷Re、橙皮苷、总黄酮和总多糖含量均得到显著性提高；参苓白术散改良方发酵液作用于GES-1细胞48 h时使细胞增殖活力提高，且中、高剂量组发酵液能降低GES-1细胞酒精性损伤的总凋亡率。

结论

参苓白术散改良方发酵后其成分影响较大，含量有明显变化，发酵工艺可行，且对GES-1细胞有增殖作用，同时能降低GES-1细胞酒精性损伤的总凋亡率。

, authors=刘红宁, 郑凯麒, 陈丽华, 姜佳恩, 周权, authorsList=刘红宁, 郑凯麒, 陈丽华, 姜佳恩, 周权, authorCompany=null, correspAuthors=陈丽华, authorNote=

刘红宁（1957-），男，江西赣县人，教授，博士研究生导师，博士，研究方向：中药药性与配伍机制。

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陈丽华（1972-），女，江西崇仁人，教授，博士研究生导师，博士，研究方向：高品质中药产品开发与评价。E-mail：。
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刘红宁（1957-），男，江西赣县人，教授，博士研究生导师，博士，研究方向：中药药性与配伍机制。

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刘红宁（1957-），男，江西赣县人，教授，博士研究生导师，博士，研究方向：中药药性与配伍机制。

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Carbohydr Polym，2016，147：401-408., articleTitle=Structural characterization and in vitro antitumor activity of an acidic polysaccharide from Angelica sinensis（Oliv. ）Diels, refAbstract=null)], funds=[Fund(id=1322923024214786726, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322923012546232908, awardId=20212AAF01005, language=CN, fundingSource=江西省科技计划“揭榜挂帅”关键技术类项目(20212AAF01005), fundOrder=null, country=null), Fund(id=1322923024290284199, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322923012546232908, awardId=CXTD-22004, language=CN, fundingSource=江西中医药大学校级科技创新团队发展计划项目(CXTD-22004), fundOrder=null, country=null)], companyList=[AuthorCompany(id=1322923016975417961, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322923012546232908, xref=null, ext=[AuthorCompanyExt(id=1322923016983806570, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322923012546232908, companyId=1322923016975417961, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=Jiangxi University of Chinese Medicine，Nanchang 330004，Jiangxi，China), AuthorCompanyExt(id=1322923016992195179, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322923012546232908, companyId=1322923016975417961, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=江西中医药大学，江西 南昌 330004)])], figs=[ArticleFig(id=1322923020590908046, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322923012546232908, language=EN, label=null, caption=null, figureFileSmall=OrbAP9W5PIgVMO6OxIrorw==, figureFileBig=Q3dPo6rb+yVhxuoJQvNkZw==, tableContent=null), ArticleFig(id=1322923020666405519, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322923012546232908, language=CN, label=图1, caption=阿氏肠杆菌发酵时长优化活性成分含量测定结果

注：A.人参皂苷Re含量；B.橙皮苷含量；C.总黄酮含量；D.总多糖含量；不同小写字母表示组间差异显著（P<0.05），相同字母为差异无统计学意义（P>0.05）。

, figureFileSmall=OrbAP9W5PIgVMO6OxIrorw==, figureFileBig=Q3dPo6rb+yVhxuoJQvNkZw==, tableContent=null), ArticleFig(id=1322923020850954896, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322923012546232908, language=EN, label=null, caption=null, figureFileSmall=1cNobGCCY3/Bz5UmwybM0A==, figureFileBig=Mq5cCeDI2gMcsDoL7tGCFQ==, tableContent=null), ArticleFig(id=1322923020926452369, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322923012546232908, language=CN, label=图2, caption=阿氏肠杆菌发酵接种量优化活性成分含量测定结果

注：A.人参皂苷Re含量；B.橙皮苷含量；C.总黄酮含量；D.总多糖含量；不同小写字母表示组间差异显著（P<0.05），相同字母为差异无统计学意义（P>0.05）。

, figureFileSmall=1cNobGCCY3/Bz5UmwybM0A==, figureFileBig=Mq5cCeDI2gMcsDoL7tGCFQ==, tableContent=null), ArticleFig(id=1322923021027115666, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322923012546232908, language=EN, label=null, caption=null, figureFileSmall=KjCDHf44U0t5BFiTsE2Utw==, figureFileBig=zq4upfT0yT8CJYQv6dTQag==, tableContent=null), ArticleFig(id=1322923021098418835, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322923012546232908, language=CN, label=图3, caption=阿氏肠杆菌发酵pH值优化活性成分含量测定结果

注：A.人参皂苷Re含量；B.橙皮苷含量；C.总黄酮含量；D.总多糖含量；不同小写字母表示组间差异显著（P<0.05），相同字母为差异无统计学意义（P>0.05）。

, figureFileSmall=KjCDHf44U0t5BFiTsE2Utw==, figureFileBig=zq4upfT0yT8CJYQv6dTQag==, tableContent=null), ArticleFig(id=1322923021182304916, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322923012546232908, language=EN, label=null, caption=null, figureFileSmall=nWhG1W9zjugHFeMRnDo8Bw==, figureFileBig=R5k5+AN0MJ0obyl4TPV3nA==, tableContent=null), ArticleFig(id=1322923021253608085, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322923012546232908, language=CN, label=图4, caption=蛹虫草真菌发酵时长优化活性成分含量测定结果

注：A.人参皂苷Re含量；B.橙皮苷含量；C.总黄酮含量；D.总多糖含量；不同小写字母表示组间差异显著（P<0.05），相同字母为差异无统计学意义（P>0.05）。

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注：A.人参皂苷Re含量；B.橙皮苷含量；C.总黄酮含量；D.总多糖含量；不同小写字母表示组间差异显著（P<0.05），相同字母为差异无统计学意义（P>0.05）。

, figureFileSmall=BqJBu2H9xq7BSgxvb9UlEQ==, figureFileBig=i2g8yVo87Vgs+cQ3Q1Cn6Q==, tableContent=null), ArticleFig(id=1322923021677232792, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322923012546232908, language=EN, label=null, caption=null, figureFileSmall=i+J2XlokDxxbsrwm/Nfg5w==, figureFileBig=oSLiBCHXI7/y4I8jXncNVg==, tableContent=null), ArticleFig(id=1322923021740147353, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322923012546232908, language=CN, label=图6, caption=蛹虫草真菌发酵pH值优化活性成分含量测定结果

注：A.人参皂苷Re含量；B.橙皮苷含量；C.总黄酮含量；D.总多糖含量；不同小写字母表示组间差异显著（P<0.05），相同字母为差异无统计学意义（P>0.05）。

, figureFileSmall=i+J2XlokDxxbsrwm/Nfg5w==, figureFileBig=oSLiBCHXI7/y4I8jXncNVg==, tableContent=null), ArticleFig(id=1322923021815644826, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322923012546232908, language=EN, label=null, caption=null, figureFileSmall=kmJR6o33dVMzWlu0tVtM2A==, figureFileBig=zjFUoxI6rQN5pDuCKQ7MDQ==, tableContent=null), ArticleFig(id=1322923021886947995, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322923012546232908, language=CN, label=图7, caption=多菌种发酵活性成分含量测定结果

注：A.人参皂苷Re含量；B.橙皮苷含量；C.总黄酮含量；D.总多糖含量；不同小写字母表示组间差异显著（P<0.05），相同字母为差异无统计学意义（P>0.05）。

, figureFileSmall=kmJR6o33dVMzWlu0tVtM2A==, figureFileBig=zjFUoxI6rQN5pDuCKQ7MDQ==, tableContent=null), ArticleFig(id=1322923021966639772, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322923012546232908, language=EN, label=null, caption=null, figureFileSmall=GzrWlAM+yUnEAXgsTMtz5A==, figureFileBig=9ULdfPifQEi19Yk0PtlQQQ==, tableContent=null), ArticleFig(id=1322923022029554333, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322923012546232908, language=CN, label=图8, caption=参苓白术散改良方水提液及发酵液含药血清对GES-1细胞增殖活力的影响

注：*与对照组相比，P<0.05。

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样品编号发酵菌种接种量/（%）接种pH值发酵时间/（d）样品编号发酵菌种接种量/（%）接种pH值发酵时间/（d）
1阿氏肠杆菌36115蛹虫草真菌363
2阿氏肠杆菌36216蛹虫草真菌364
3阿氏肠杆菌36317蛹虫草真菌365
4阿氏肠杆菌36418蛹虫草真菌366
5阿氏肠杆菌36519蛹虫草真菌163
6阿氏肠杆菌16120蛹虫草真菌563
7阿氏肠杆菌56121蛹虫草真菌763
8阿氏肠杆菌76122蛹虫草真菌353
9阿氏肠杆菌15123蛹虫草真菌373
10阿氏肠杆菌17124蛹虫草真菌383
11阿氏肠杆菌18125蛹虫草真菌393
12阿氏肠杆菌19126接种阿氏肠杆菌，24h后接种蛹虫草真菌
13蛹虫草真菌36127接种蛹虫草真菌，72h后接种阿氏肠杆菌
14蛹虫草真菌362
), ArticleFig(id=1322923022310572706, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322923012546232908, language=CN, label=表1, caption=

发酵条件单因素优化实验设计

, figureFileSmall=null, figureFileBig=null, tableContent=
样品编号发酵菌种接种量/（%）接种pH值发酵时间/（d）样品编号发酵菌种接种量/（%）接种pH值发酵时间/（d）
1阿氏肠杆菌36115蛹虫草真菌363
2阿氏肠杆菌36216蛹虫草真菌364
3阿氏肠杆菌36317蛹虫草真菌365
4阿氏肠杆菌36418蛹虫草真菌366
5阿氏肠杆菌36519蛹虫草真菌163
6阿氏肠杆菌16120蛹虫草真菌563
7阿氏肠杆菌56121蛹虫草真菌763
8阿氏肠杆菌76122蛹虫草真菌353
9阿氏肠杆菌15123蛹虫草真菌373
10阿氏肠杆菌17124蛹虫草真菌383
11阿氏肠杆菌18125蛹虫草真菌393
12阿氏肠杆菌19126接种阿氏肠杆菌，24h后接种蛹虫草真菌
13蛹虫草真菌36127接种蛹虫草真菌，72h后接种阿氏肠杆菌
14蛹虫草真菌362
), ArticleFig(id=1322923024000877220, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322923012546232908, language=EN, label=null, caption=null, figureFileSmall=null, figureFileBig=null, tableContent=
样品编号人参皂苷Re含量（）/（mg·g原药材-1）橙皮苷含量（）/（mg·g原药材-1）总黄酮含量（）/（mg·g原药材-1）总多糖含量/（mg·g原药材-1）综合评分/（分）
15.261±0.1241.592±0.0042.399±0.08681.543±1.08194.09
26.410±0.6271.454±0.0242.346±0.00551.053±2.08986.34
34.569±0.5641.307±0.2062.351±0.01142.434±1.11874.14
43.874±0.2031.299±0.0272.545±0.03538.295±1.46171.84
52.201±0.1381.207±0.0292.351±0.03537.058±0.81861.46
610.152±0.2320.658±0.0112.823±0.13590.737±2.59884.94
74.879±0.2311.599±0.0232.394±0.06587.961±0.60982.12
85.531±0.1331.580±0.0182.326±0.04092.001±2.00083.92
96.184±0.0841.640±0.0372.465±0.031131.180±0.16087.06
104.257±0.1011.438±0.1061.851±0.01876.379±1.13363.34
11--1.939±0.01870.585±0.21830.62
12--2.379±0.01773.138±0.27235.01
136.206±0.0161.663±0.0113.798±0.042126.243±1.07390.37
146.494±0.3151.706±0.0363.653±0.056137.466±4.04193.06
156.536±0.2161.697±0.0213.823±0.067146.813±1.04795.73
165.864±0.0441.737±0.0303.812±0.039123.138±1.79089.58
176.534±0.3201.816±0.0094.068±0.091126.584±0.39895.60
185.787±0.0301.908±0.0934.045±0.056125.078±0.53493.29
196.034±0.1651.716±0.0173.414±0.039145.046±1.32593.40
205.614±0.1071.608±0.0233.669±0.113141.826±0.43491.21
214.582±0.1261.600±0.0244.139±0.218135.860±3.19688.97
225.443±0.0051.848±0.0443.423±0.069116.806±1.23386.81
235.804±0.0831.613±0.0263.128±0.024125.168±0.40884.42
246.345±0.0241.414±0.0093.178±0.035112.911±2.74981.92
256.966±0.2060.632±0.0273.377±0.072113.101±1.57874.89
266.632±0.0651.816±0.0534.276±0.068146.910±1.64596.98
277.512±0.2861.824±0.0083.952±0.128143.809±3.30097.60
水提组2.590±0.0241.520±0.0082.801±0.010122.782±1.239-
), ArticleFig(id=1322923024093151909, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322923012546232908, language=CN, label=表2, caption=

菌种发酵条件单因素优化实验活性成分含量测定结果

, figureFileSmall=null, figureFileBig=null, tableContent=
样品编号人参皂苷Re含量（）/（mg·g原药材-1）橙皮苷含量（）/（mg·g原药材-1）总黄酮含量（）/（mg·g原药材-1）总多糖含量/（mg·g原药材-1）综合评分/（分）
15.261±0.1241.592±0.0042.399±0.08681.543±1.08194.09
26.410±0.6271.454±0.0242.346±0.00551.053±2.08986.34
34.569±0.5641.307±0.2062.351±0.01142.434±1.11874.14
43.874±0.2031.299±0.0272.545±0.03538.295±1.46171.84
52.201±0.1381.207±0.0292.351±0.03537.058±0.81861.46
610.152±0.2320.658±0.0112.823±0.13590.737±2.59884.94
74.879±0.2311.599±0.0232.394±0.06587.961±0.60982.12
85.531±0.1331.580±0.0182.326±0.04092.001±2.00083.92
96.184±0.0841.640±0.0372.465±0.031131.180±0.16087.06
104.257±0.1011.438±0.1061.851±0.01876.379±1.13363.34
11--1.939±0.01870.585±0.21830.62
12--2.379±0.01773.138±0.27235.01
136.206±0.0161.663±0.0113.798±0.042126.243±1.07390.37
146.494±0.3151.706±0.0363.653±0.056137.466±4.04193.06
156.536±0.2161.697±0.0213.823±0.067146.813±1.04795.73
165.864±0.0441.737±0.0303.812±0.039123.138±1.79089.58
176.534±0.3201.816±0.0094.068±0.091126.584±0.39895.60
185.787±0.0301.908±0.0934.045±0.056125.078±0.53493.29
196.034±0.1651.716±0.0173.414±0.039145.046±1.32593.40
205.614±0.1071.608±0.0233.669±0.113141.826±0.43491.21
214.582±0.1261.600±0.0244.139±0.218135.860±3.19688.97
225.443±0.0051.848±0.0443.423±0.069116.806±1.23386.81
235.804±0.0831.613±0.0263.128±0.024125.168±0.40884.42
246.345±0.0241.414±0.0093.178±0.035112.911±2.74981.92
256.966±0.2060.632±0.0273.377±0.072113.101±1.57874.89
266.632±0.0651.816±0.0534.276±0.068146.910±1.64596.98
277.512±0.2861.824±0.0083.952±0.128143.809±3.30097.60
水提组2.590±0.0241.520±0.0082.801±0.010122.782±1.239-
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刘红宁 , 郑凯麒 , 陈丽华 , 姜佳恩 , 周权
中华中医药学刊 | 博士导师新论 2025,43(3): 6-12
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中华中医药学刊 |博士导师新论 2025 , 43 (3) : 6 -12
参苓白术散改良方发酵工艺及对GES-1细胞酒精性损伤的干预作用研究
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刘红宁, 郑凯麒, 陈丽华 , 姜佳恩, 周权
作者信息
  • 江西中医药大学，江西 南昌 330004
通讯作者:
陈丽华（1972-），女，江西崇仁人，教授，博士研究生导师，博士，研究方向：高品质中药产品开发与评价。E-mail：。
作者简介:

刘红宁（1957-），男，江西赣县人，教授，博士研究生导师，博士，研究方向：中药药性与配伍机制。

Study on Fermentation Technology of Shenling Baizhu Powder（参苓白术散）and Its Intervention Effect on Alcohol Injury of GES-1 Cells
Hongning LIU, Kaiqi ZHENG, Lihua CHEN , Jiaen JIANG, Quan ZHOU
Affiliations
  • Jiangxi University of Chinese Medicine，Nanchang 330004，Jiangxi，China
出版时间: 2025-03-10 doi: 10.13193/j.issn.1673-7717.2025.03.002
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摘要
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目的

研究阿氏肠杆菌和蛹虫草真菌发酵参苓白术散改良方最佳发酵条件，并在细胞层面评价发酵液的安全性和对酒精性胃损伤治疗的有效性。

方法

实验检测经不同条件发酵后的参苓白术散改良方发酵液中功能成分的含量，并进行综合评价，在此基础上利用单因素实验对发酵条件进行优化；以酒精作用24 h建立GES-1细胞酒精性损伤模型，考察参苓白术散改良方水提液（0.938 mg/g）和发酵液在低、中、高剂量（0.469、0.938、1.876 mg/g）对人胃黏膜上皮细胞株GES-1细胞酒精性损伤基础凋亡的干预作用。

结果

阿氏肠杆菌发酵参苓白术散改良方的最佳发酵工艺为pH值5，接种量1%，发酵时间1 d。蛹虫草真菌发酵参苓白术散改良方的最佳发酵工艺为pH值6，接种量3%，发酵时间3 d。多菌种发酵最佳接种顺序为先接种阿氏肠杆菌。最佳发酵工艺下人参皂苷Re、橙皮苷、总黄酮和总多糖含量均得到显著性提高；参苓白术散改良方发酵液作用于GES-1细胞48 h时使细胞增殖活力提高，且中、高剂量组发酵液能降低GES-1细胞酒精性损伤的总凋亡率。

结论

参苓白术散改良方发酵后其成分影响较大，含量有明显变化，发酵工艺可行，且对GES-1细胞有增殖作用，同时能降低GES-1细胞酒精性损伤的总凋亡率。

微生物  /  发酵  /  GES-1细胞  /  酒精性胃损伤
Objective

To study the optimal fermentation conditions of Enterobacter albieri and Cordyceps pupae for Shenling Baizhu Powder（参苓白术散），and evaluate the safety and efficacy of the fermentation solution on alcoholic gastric injury at cell level.

Methods

In this experiment，the contents of functional components in fermentation broth of the modified formula of Shenling Baizhu Powder after fermentation under different conditions were measured and comprehensively evaluated. The alcoholic gastric injury model of GES-1 cells was established after 24 h of alcohol intervention. The intervention effects of water extract（0.938 mg/g）and fermentation broth of the modified formula of Shenling Baizhu Powder at low，medium and high doses（0.469 mg/g，0.938 mg/g，1.876 mg/g）on basic apoptosis of GES-1 cells were investigated.

Results

The optimal fermentation process of Enterobacter asburiae for Shenling Baizhu Powder was pH value 5，inoculation amount 1% and fermentation time1 day. The optimal fermentation process of Shenling Baizhu Powder was pH 6，inoculation amount 3% and fermentation time 3 days. The best inoculation sequence for multistrain fermentation was to vaccinate Enterobacter asburiae first. The contents of ginsenoside Re，hesperidin，total flavonoids and total polysaccharides were significantly increased under the optimal fermentation process. The fermentation solution of Shenling Baizhu Powder improved GES-1 cells at 48 h，and the fermentation solution of medium and high dose groups could reduce the total apoptosis rate of GES-1 cells.

Conclusion

After fermentation，the composition of the modified formula of Shenling Baizhu Powder had a great influence，the content changed obviously and the fermentation process was feasible. It had a proliferation effect on GES-1 cells，and at the same time it could reduce the overall apoptosis rate of GES-1 cells.

microorganism  /  fermentation  /  GES-1 cells  /  alcoholic stomach injury
刘红宁, 郑凯麒, 陈丽华, 姜佳恩, 周权. 参苓白术散改良方发酵工艺及对GES-1细胞酒精性损伤的干预作用研究. 中华中医药学刊, 2025 , 43 (3) : 6 -12 . DOI: 10.13193/j.issn.1673-7717.2025.03.002
Hongning LIU, Kaiqi ZHENG, Lihua CHEN, Jiaen JIANG, Quan ZHOU. Study on Fermentation Technology of Shenling Baizhu Powder（参苓白术散）and Its Intervention Effect on Alcohol Injury of GES-1 Cells[J]. Chinese Archives of Traditional Chinese Medicine, 2025 , 43 (3) : 6 -12 . DOI: 10.13193/j.issn.1673-7717.2025.03.002
正文
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目前临床上用于治疗胃黏膜损伤的药物主要有铝制剂以及铋剂等，但存在着不良反应大、易复发和需长期使用等缺点[1]。随着健康中国2030启动，研究开发具备养胃、护胃功效的药食同源产品，对于降低饮酒导致的胃部相关疾病的发病率具有重要意义。活性氧是引起胃黏膜酒精性损伤的重要因素之一[2-4]。中药复方通过抗氧化应激、控制炎症因子等多重作用协同发挥药效，体现了中草药治疗疾病多靶点、整体调节的优势[5]。参苓白术散改良方是由人参、茯苓、陈皮、白扁豆、山药、甘草、莲子、砂仁、薏苡仁和桔梗十味药食同源中药组成，含有黄酮、多糖和皂苷类成分，有研究发现[6-7]橙皮苷不仅能直接地抑制氧化酶活性，还可以通过减少中性粒细胞的迁移，增强胃黏膜旁黏液的保护屏障；人参皂苷Re对钙离子稳态、线粒体氧化应激等具有保护作用[8]，同时还有一定的抗胃癌作用[9]；多糖类、黄酮类、萜类和生物碱类等物质是具有抗酒精性胃损伤功能中草药的活性成分[10]。黄酮类物质可通过影响胃组织中生物酶的表达，抑制炎症反应，同时减轻乙醇和脂质过氧化引起的自由基损伤，共同起到保护胃黏膜的作用，且对酒精性胃损伤的预防具有积极作用[11]。同时多糖类成分能多方位地调节抗氧化剂和炎症因子的水平、促进相关防御因子的释放和降低血清中组胺水平等方式有效地保护酒精性胃损伤[12]。中药材经微生物发酵后能提高多糖类、黄酮类、皂苷类等有效成分含量并改善肠道四大屏障，调节菌群及代谢，维持肠道稳态[13-14]，且发酵后中药的抗氧化、抗炎、抑菌、降血脂等作用显著增强[15]，同时补益类中药经微生物发酵后，更容易被人体吸收，营养价值和药效更高[16]。有研究表明中药复方同样也可以利用微生物进行发酵，且发酵后的中药复方疗效显著性提高[17-18]。
基于此，采用液态发酵参苓白术散改良方，以人参皂苷Re、橙皮苷、总黄酮和总多糖含量为指标成分，比较发酵前后参苓白术散改良方中指标成分的变化，并在细胞层面评价发酵液的安全性和有效性。
1　材料与仪器
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1.1　主要材料与试剂
人参、砂仁、薏苡仁、桔梗、白扁豆、茯苓、山药、陈皮、甘草、莲子（购自江中饮片厂）；阿氏肠杆菌（分离自江西中医药大学）；蛹虫草真菌（购自北纳创联生物技术研究院）；橙皮苷对照品（购自成都普菲德生物技术有限公司）；人参皂苷Re对照品（购自维克奇生物科技有限公司）；乙腈、甲醇（购自美国sigma公司）；人胃黏膜上皮细胞株GES-1细胞（购自中国上海细胞库）；Cell Counting Kit-8、Annexin V-AbFluorTM488/PI（购自亚科因生物技术有限公司）；DMEO高糖培养基、胰蛋白酶-EDTA消化液、PBS缓冲液、胰蛋白酶-消化液（无EDTA）、芦丁对照品、D（+）-无水葡萄糖对照品（购自北京索莱宝科技有限公司）；胎牛血清（购自上海逍鹏生物科技有限公司）；细胞冻存液（购自苏州海星生物科技有限公司）；氢氧化钠；亚硝酸钠；三氯化铝；无水乙醇；浓硫酸；苯酚；马铃薯葡萄糖肉汤；马铃薯葡萄糖琼脂；MRS肉汤；MRS琼脂；营养琼脂；营养肉汤；沙氏葡萄糖液体培养基；沙氏葡萄糖琼脂。
1.2　主要仪器
CJ-SL20I超纯水仪（四川纯洁科技有限公司）；DZTW电热套（永光明医疗仪器有限公司）；KQ-500VDE双频数控超声清洗器（昆山超声仪器有限公司）；OSB-2200油浴锅（上海爱朗仪器有限公司）；CCP5-Mini低温冷却循环机（大龙兴创实验仪器公司）；NVP-1000隔膜真空泵（上海爱朗仪器有限公司）；C-15R冷冻高速离心机（杰懋万得福生物科技有限公司）；DZKW-S-8水浴锅（永光明医疗仪器有限公司）；T6新世纪紫外-可见分光光度计（北京普析通用仪器有限责任公司）；SCI-VS可调式混匀仪（美国scilogex公司）；THZ-300C恒温培养摇床（上海一恒科学仪器有限公司）；SW-CJ-2F双人超净工作台（苏州安泰空气技术有限公司）；GI54DS高压蒸汽灭菌器（致徽厦门仪器有限公司）；BT25S电子天平（赛多利斯科学仪器北京有限公司）；ICES/NMB-001高效液相色谱仪（美国安捷伦公司）；TSZ4-WS低速离心机（湖南湘仪实验室仪器开发有限公司）；Forma Series Ⅱ CO2细胞培养箱（美国Thermo Fisher公司）；SPARK 10M多功能酶标仪（瑞士Tecan公司）；CKX41倒置相差显微镜（日本Olympus公司）；DHG-9246A电热恒温鼓风干燥箱（上海精宏实验设备公司）；Attune NxT流式细胞仪（美国Thermo Fisher公司）。
2　实验方法
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2.1　参苓白术散改良方的发酵工艺
工艺流程：中药饮片→纯水提取→灭菌→接菌→发酵→灭菌→检测。
2.1.1　发酵步骤
分别称取人参（人工种植3年）、茯苓、白扁豆、橘皮、山药、甘草、莲子、砂仁、薏苡仁、桔梗（比例关系：5∶20∶5∶10∶20∶10∶20∶1∶5∶5），加入10倍体积水中，煎煮2 h冷却后经纱布过滤上清液，将残渣加入8倍体积水中，继续煎煮1.5 h冷却后经纱布过滤上清液，合并两次上清液，浓缩至0.5 g/mL，制成参苓白术散改良方提取液。取20 mL提取液于50 mL锥形瓶中，调节pH，121℃高压蒸汽灭菌30 min，冷却至室温后，按一定接种量接入单一菌种菌悬液，将锥形瓶置于恒温培养箱或恒温摇床中进行发酵。
2.1.2　菌种发酵条件
（1）阿氏肠杆菌发酵条件：固定发酵温度为37℃。
（2）蛹虫草真菌发酵条件：固定发酵温度为28℃，转速125 r/min。
2.2　发酵条件单因素优化实验
考察发酵时长、接种量和pH值对阿氏肠杆菌发酵参苓白术散改良方的影响。考察发酵时长、接种量和pH值对蛹虫草真菌发酵参苓白术散改良方的影响。考察接种顺序对多菌种发酵参苓白术散改良方的影响。先后接入单一菌种（阿氏肠杆菌或蛹虫草真菌）菌悬液，按其最佳发酵条件进行发酵，待发酵完成后，再接种另一种菌种按其最佳发酵条件进行发酵。实验设计见表1。
2.3　含量测定
2.3.1　供试品溶液的制备
待发酵完成后，121℃高压蒸汽灭菌30 min，待锥形瓶冷却至室温后，以5000 r/min，4℃，离心10 min，吸取上层清液，再以相同条件离心10 min，两次离心后合并上清液，于4℃储存备用。
2.3.2　发酵前后人参皂苷Re和橙皮苷的含量测定
（1）液相条件：色谱柱：菲罗门Titank C18柱（250 mm×4.6 mm，5 μm），流动相：乙腈（A）-水（B），洗脱梯度（0～12 min，19%A；12～25 min，19%～23%A；25～26 min，23%～29%A；26～55 min，29%～32%A），流速：1 mL/min，柱温：35℃，检测波长：203 nm，进样量10μL。
（2）人参皂苷Re标准曲线的制定：取浓度为2.012、1.006、0.503、0.252、0.126 mg·mL-1的人参皂苷Re对照品溶液，根据“2.3.2”项的液相条件进样，计算峰面积，以峰面积为纵坐标，人参皂苷Re浓度为横坐标，建立标准曲线，回归方程：y＝2 515.6x+44.35，r＝0.999 4。
（3）橙皮苷标准曲线的制定：取浓度为0.506、0.253、0.127、0.064、0.032 mg·mL-1的橙皮苷对照品溶液，根“2.3.2”项的液相条件进样，计算峰面积，以峰面积为纵坐标，橙皮苷浓度为横坐标，建立标准曲线，回归方程：y＝41 494x-114.52，r＝0.999 6。
（4）含量测定：将供试品用超纯水稀释5倍，用0.22μm微孔滤膜过滤后按“2.3.2”项下液相条件进样，测定其峰面积，根据回归方程计算样品中人参皂苷Re、橙皮苷的含量，乘以稀释倍数，结果表示为mg·g原药材-1。人参皂苷Re和橙皮苷含量按公式计算：
X1=C1×n1/A
X1：发酵液中人参皂苷Re或橙皮苷含量（mg·g原药材-1）；n1：试样溶液的稀释倍数；C1：由标准曲线计算得出的待测样品溶液的样品浓度（mg/mL）；A：提取液浓度（0.5 g/mL）。
2.3.3　发酵前后总多糖的含量测定
（1）标准曲线的制定：精密称定无水葡萄糖对照品2.5 mg，加水于5 mL容量瓶定容于刻度线，配制成0.5 mg·mL-1的葡萄糖标准溶液。精密量取对照品溶液0、0.2、0.4、0.6、0.8、1 mL，加水补充体积至1 mL，精密加入5%苯酚溶液1 mL（临用配制），摇匀，再精密加入浓硫酸5 mL，摇匀，置沸水浴中加热20 min，取出后置冰浴中冷却5 min，置于紫外-可见分光光度计中，在488 nm的波长处测定吸光度，以吸光度为纵坐标，浓度为横坐标，建立标准曲线，回归方程：y＝57.714x+0.046 6，r＝0.999 1。
（2）含量测定：将供试液稀释400倍，取稀释后的样品1 mL，置于10 mL具塞试管中，同“2.3.3”项下方法，自“精密加入5%苯酚溶液1 mL”起，依法测定吸光度，带入标准曲线得待测样品溶液的多糖浓度。
总多糖含量按公式计算：
X2=C2×n2×400/A
X2：发酵液中总多糖含量（mg·g原药材-1）；n2：试样溶液的稀释倍数；A：提取液浓度（0.5g/mL）；C2：由标准曲线计算得出的待测样品溶液的多糖浓度（mg/mL）；400：待测液稀释倍数。
2.3.4　发酵前后总黄酮的含量测定
（1）标准曲线的制定：吸取浓度为1 mg·mL-1芦丁标准溶液0、0.4、1、0.8、1.6、2.0 mL分别置于10 mL的棕色容量瓶中，加60%乙醇溶液至5.0 mL，加0.3 mL 5%亚硝酸钠溶液，混匀后静置6 min；再加入0.3 mL 10%的三氯化铝溶液，摇匀后静置8 min；加4 mL 4%的氢氧化钠溶液，用60%乙醇溶液定容至刻度，摇匀后静置8 min。在510 nm波长处测定吸光度值，以未加芦丁标准液的溶液为空白，横坐标为芦丁标准液浓度，纵坐标为吸光度值，绘制标准曲线。回归方程：y＝9.780 5x-0.027 9，r＝0.999 1。
（2）含量测定：将供试液稀释5倍，取稀释后的样品1 mL，置于10 mL具塞试管中，同“2.3.4”项下方法，加入试剂同上。以未加氢氧化钠试剂的溶液为空白，在510 nm波长处测定吸光度值，带入标准曲线得待测样品溶液的黄酮浓度。
总黄酮含量按公式计算：
X3=C3×n3×5/A
X3：发酵液中总黄酮含量（mg·g原药材-1）；n3：试样溶液的稀释倍数；A：提取液浓度（0.5 g/mL）；C3：由标准曲线计算得出的待测样品溶液的黄酮浓度（mg/mL）；5：待测液稀释倍数。
2.4　发酵液评分标准建立
本实验分别对参苓白术散改良方发酵液的人参皂苷Re、橙皮苷、总黄酮和总多糖含量4个指标进行测定。按照综合评分的方法确定参苓白术散改良方适宜发酵菌种以及发酵条件优化，评分方案如下。
人参皂苷Re得分=人参皂苷Re含量/表中人参皂苷Re最高量×0.25×100
橙皮苷得分=橙皮苷含量/表中橙皮苷含量最高量×0.25×100
总黄酮得分=总黄酮含量/表中总黄酮含量最高量×0.25×100
总多糖得分=总多糖含量/表中总多糖含量最高量×0.25×100
综合评分=人参皂苷Re得分+橙皮苷得分+总黄酮得分+总多糖得分
2.5　细胞实验
2.5.1　CCK-8法检测不同浓度参苓白术散改良方发酵液对GES-1细胞增殖情况的影响
取生长良好的GES-1，PBS清洗2次，消化计数，按每孔8×103接种至96孔板，分组设为空白组、对照组、水提组、低剂量组、中剂量组和高剂量组，每组设6个复孔，每孔液体体积为100μL；将接种好的96孔板分别培养24、48、72、96 h，按照CCK-8检测试剂盒说明书提示操作，检测波长设为450 nm，用多功能酶标仪检测空白孔、对照孔和实验孔吸光度，重复3次。
2.5.2　流式细胞术检测不同浓度参苓白术散改良方发酵液对酒精干预后GES-1细胞基础凋亡状况的影响
取生长良好的GES-1细胞，PBS清洗2次，消化计数，按每孔2×105接种至6孔板中，先用含3%酒精的完全培养基处理24 h，加药干预，设置正常对照组、模型组、水提组、低剂量组、中剂量组和高剂量组，每组设3个复孔，每孔液体体积为2 mL，将6孔板培养48 h，按双染凋亡检测试剂盒操作，流式细胞仪检测，FlowJo_V10软件分析，重复3次
3　实验结果
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3.1　菌种发酵条件单因素优化实验结果
各指标含量测定结果以及评价得分见表2。
3.1.1　阿氏肠杆菌发酵时长优化结果分析
如表2和图1所示，与水提组相比，经阿氏肠杆菌发酵1～4 d时人参皂苷Re含量增多，在发酵2 d时增幅最大；橙皮苷含量在发酵1 d后增加，发酵2～5 d后随发酵时间增长不断减少；总黄酮含量经发酵后减少；总多糖含量随发酵时长增加而减少。根据评分标准得出阿氏肠杆菌发酵组中第1组得分最高，所以认定发酵1 d为阿氏肠杆菌最佳发酵时间。
3.1.2　阿氏肠杆菌发酵接种量优化结果分析
如表2和图2所示，在接种量优化实验中，与水提组相比，在1%接种量时人参皂苷含量、总黄酮含量增多；总多糖含量较水提液均减少；接种量仅在1%时橙皮苷含量减少。根据阿氏肠杆菌接种量优化组中总评分可看出第6组得分最高，故阿氏肠杆菌接种量为1%时为其最适接种量。
3.1.3　阿氏肠杆菌发酵pH值优化结果分析
如表2和图3所示，在pH值优化实验中，pH值在5～7时人参皂苷Re含量增多，且pH值在6时增加最多；pH值在5时橙皮苷含量不变，pH值在6～9时减少；pH值在5～7时，总黄酮含量降低；pH值在5时总多糖含量增多。根据阿氏肠杆菌pH值优化组中总评分可看出第9组得分最高，阿氏肠杆菌的最适发酵条件为pH值为5的酸性环境。
因此，阿氏肠杆菌的发酵参苓白术散改良方最适条件为在pH为5的条件下，以1%接种量接种后发酵1 d。
3.1.4　蛹虫草真菌发酵时长优化结果分析
如表2和图4所示，与水提组相比，经蛹虫草真菌发酵1～6 d后使人参皂苷Re含量增多；橙皮苷含量在发酵2～6d时增多，且随发酵时长增大而增多；总黄酮含量在经发酵后增多，随发酵时长增大而增多；发酵2～3 d时总多糖含量提升，在发酵3 d时增幅最大。根据评分标准得出蛹虫草真菌发酵组中第15组得分最高，因此认定发酵3 d为蛹虫草真菌最佳发酵时间。
3.1.5　蛹虫草真菌发酵接种量优化结果分析
如表2和图5所示，在接种量优化实验中，与水提组相比，人参皂苷Re含量均增加；在3%接种蛹虫草真菌时人参皂苷Re含量上升最多；橙皮苷含量1%和3%接种量有显著性提高；总黄酮含量均增加，且随接种量加大而增多；总多糖含量亦增多，接种量在1%和3%时组间显著性增多。根据蛹虫草真菌接种量优化组中总评分可看出第15组得分最高，蛹虫草真菌接种量为3%时为其最适接种量。
3.1.6　蛹虫草真菌发酵pH值优化结果分析
如表2和图6所示，在pH优化实验中，与水提组相比，人参皂苷Re含量增多，且随pH值增大而不断增加；橙皮苷含量在pH值为5～7时增多，随pH值增大而减少；总黄酮含量增多，pH值在6时增幅最大；总多糖含量仅在pH值为6时增多。根据蛹虫草真菌pH值优化组中总评分可看出仍是第15组得分最高，故蛹虫草真菌的最适发酵条件为pH值为6的弱酸性环境。因此，蛹虫草真菌的发酵参苓白术散改良方最适条件为在pH为6的条件下，以3%接种量接种后发酵3 d。
3.1.7　多菌种发酵菌种接种顺序结果分析
如表2和图7所示，在多菌种发酵实验中，与水提组相比，多菌种发酵各项指标显著提升。先接种蛹虫草真菌的多菌种发酵组较其他组人参皂苷Re含量有显著性提升；橙皮苷含量较阿氏肠杆菌发酵组均有显著性提升，较蛹虫草发酵组无显著性提升；先接种阿氏肠杆菌的多菌种发酵组总黄酮含量增幅最大；多菌种发酵组和蛹虫草发酵组总多糖含量均高于阿氏肠杆菌发酵组，且多菌种发酵组的主要活性成分含量增强率优于单菌种发酵组。根据评分标准得出第26组得分最高，先接种阿氏肠杆菌为多菌种发酵的接种顺序。
3.2　细胞实验结果
3.2.1　不同浓度参苓白术散改良方发酵液对GES-1细胞增殖情况的影响
与对照组相比较，水提组和低、中、高剂量组参苓白术散改良方发酵液含药血清在孵育48 h均能促进GES-1细胞的增殖活力，但在低、高剂量组效果显著，其中低剂量组发酵液在孵育48 h时对细胞增殖活力具有明显的促进作用，且对细胞增殖活力效果最好。水提组和低、高剂量组发酵液在孵育72 h能促进GES-1细胞的增殖活力，但是相比于48 h促进细胞增殖效果随作用时间增长而衰退，其结果说明参苓白术散改良方及其发酵液的安全性和有效性，但值得注意的是中剂量在作用72 h后细胞增殖活力相比于对照组低，如插页Ⅰ图8所示。
3.2.2　不同浓度参苓白术散改良方发酵液对酒精干预后GES-1细胞基础凋亡状况的影响
经FlowJo-V10分析得到的流式细胞术检测GES-1细胞基础凋亡状况的结果表明，模型组凋亡率比正常组增加（P<0.05）；与模型组相比参苓白术散改良方水提组早期凋亡率有降低（P<0.05），但晚期凋亡率增加（P<0.05）；中、高剂量组参苓白术散改良方发酵液早期凋亡率显著性降低（P<0.05），高剂量组晚期凋亡率几乎不变，其余组晚期凋亡率有显著性增加，中、高剂量总凋亡率在13.63%～17.43%，差异有统计学意义（P<0.05），表明中、高剂量的参苓白术散改良方发酵液能够有效降低GES-1细胞酒精性损伤的总凋亡率，如插页Ⅰ图9所示。
4　结论与讨论
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经蛹虫草真菌发酵后黄酮含量增加，其原因可能是蛹虫草这类真菌自身含有一定量的黄酮类物质，在发酵的过程中不断积累，高压灭菌处理后使子实体内黄酮类物质溢出，从而提高了总黄酮含量。阿氏肠杆菌使总多糖含量减少，多糖被消耗可能是因为随着发酵的进行，微生物不断生长利用并分解糖类物质[19]。蛹虫草中菌丝体中较其他部位多糖含量最大[20]，而在液体发酵中蛹虫草真菌产生大量菌丝体，从而仅蛹虫草真菌使总多糖含量增多，同时发酵过程中还可能产生具有还原力且能显著提升自由基清除能力的氨基酸和抗氧化维生素等物质[21]。
参苓白术散改良方发酵液对酒精干预后GES-1细胞基础凋亡状况影响结果显示，参苓白术散改良方发酵液的中、高剂量组对GES-1细胞酒精性损伤的凋亡率有显著提高，且高剂量组对凋亡率改善最大。这可能是由于参苓白术散改良方发酵后主要活性物质提升带来的影响，且中、高剂量组降低GES-1细胞酒精性损失主要是降低其早期凋亡率，有研究表明黄酮类可减少早期细胞凋亡，减少细胞内活性氧、丙二醛含量，且能够降低GES-1细胞的氧化应激[22]，且大量的实验研究证明多糖有助于增强细胞膜的紧密性，抑制氧化应激，修复细胞损伤[23-25]。通过微生物转化制备了一种活性指标含量更高且抗酒精性胃损伤功能更有效的发酵液，并从细胞层面验证了其安全性和有效性，给这类健康产品研发带来新思路。
基金
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  • 江西省科技计划“揭榜挂帅”关键技术类项目(20212AAF01005)
  • 江西中医药大学校级科技创新团队发展计划项目(CXTD-22004)
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2025年第43卷第3期
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doi: 10.13193/j.issn.1673-7717.2025.03.002
  • 首发时间：2026-10-30
  • 出版时间：2025-03-10
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基金
江西省科技计划“揭榜挂帅”关键技术类项目(20212AAF01005)
江西中医药大学校级科技创新团队发展计划项目(CXTD-22004)
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    江西中医药大学，江西 南昌 330004

通讯作者:

陈丽华（1972-），女，江西崇仁人，教授，博士研究生导师，博士，研究方向：高品质中药产品开发与评价。E-mail：。
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2种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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