Article(id=1322922866353779548, tenantId=1146029695717560320, journalId=1255847919539208197, issueId=1322922680609014711, articleNumber=null, orderNo=null, doi=10.13193/j.issn.1673-7717.2025.02.050, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=null, receivedDateStr=null, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1793338990057, onlineDateStr=2026-10-30, pubDate=1739116800000, pubDateStr=2025-02-10, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1793338990057, onlineIssueDateStr=2026-10-30, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1793338990057, creator=13701087609, updateTime=1793338990057, updator=13701087609, issue=Issue{id=1322922680609014711, tenantId=1146029695717560320, journalId=1255847919539208197, year='2025', volume='43', issue='2', pageStart='1', pageEnd='258', issueExtLink='null', onlineDate='null', pubDate='1739116800000', pubDateStr='2025-02-10', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=0, createTime=1793338945772, creator='13701087609', updateTime=1793340618065, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1322929694785536732, tenantId=1146029695717560320, journalId=1255847919539208197, issueId=1322922680609014711, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1322929694785536733, tenantId=1146029695717560320, journalId=1255847919539208197, issueId=1322922680609014711, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=241, endPage=245, ext={EN=ArticleExt(id=1322922866542523229, articleId=1322922866353779548, tenantId=1146029695717560320, journalId=1255847919539208197, language=EN, title=Research Progress on Extraction，Separation，Purification and Pharmacological Activities of Pigments Extracted from Gouqi（Lycii Fructus）, columnId=1256183365644968140, journalTitle=Chinese Archives of Traditional Chinese Medicine, columnName=Platform of Provincial Project, runingTitle=null, highlight=null, articleAbstract=

Gouqi（Lycii Fructus）is a Chinese drug which is homology of medicine and food with the effect of nourishing liver and kidney，nourishing the essence and brightening the eyes. Pigment is one of the main active components of Gouqi（Lycii Fructus），which has pharmacological activities such as antioxidant，anti-tumor，vision protection and liver protection effects. Different extraction and purification methods have varying degrees of impact on the contents and purities of pigments. This paper summarized the extraction methods，separation and purification，chemical composition and main pharmacological activities of pigments from Gouqi（Lycii Fructus）and prospected their research prospects，providing theoretical support for the further development and utilization of pigments from Gouqi（Lycii Fructus）.

, authors=Yuanjin SU¹, Jiayu XI¹, Shuqing DONG², Shijun SHAO², Qing LIANG², Fude YANG¹, authorsList=Yuanjin SU, Jiayu XI, Shuqing DONG, Shijun SHAO, Qing LIANG, Fude YANG, authorCompany=null, correspAuthors=Qing LIANG, Fude YANG, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1322922867276526432, articleId=1322922866353779548, tenantId=1146029695717560320, journalId=1255847919539208197, language=CN, title=枸杞色素提取、分离纯化及其药理作用研究进展, columnId=1256183369033965799, journalTitle=中华中医药学刊, columnName=省级项目平台, runingTitle=null, highlight=null, articleAbstract=

枸杞是具有滋补肝肾、益精明目之效的药食同源中药。枸杞色素是枸杞的主要活性成分之一，有抗氧化、抗肿瘤、保护视力、保护肝脏等药理活性，不同的提取和分离纯化方法对枸杞色素的含量和纯度均有不同程度的影响。对枸杞色素的提取、分离纯化方法、化学组成及主要药理活性进行归纳总结，并对其研究前景进行展望，为枸杞色素进一步开发利用提供理论依据。

, authors=苏圆锦¹, 奚佳玉¹, 董树清², 邵士俊², 梁卿², 杨扶德¹, authorsList=苏圆锦, 奚佳玉, 董树清, 邵士俊, 梁卿, 杨扶德, authorCompany=null, correspAuthors=梁卿, 杨扶德, authorNote=, correspAuthorsNote=, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=vNKvek/10dvQcSm4BhVtYw==, magXml=8eqZ47Q5sOTwsca1+bzOkA==, pdfUrl=null, pdf=v90ujnJ6lgcCUmwqLZTjHA==, pdfFileSize=1776932, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=6efGv2VTXjHGeSOFV21pIg==, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=67SWmq0icHt2rtl1o/7M0A==, mapNumber=null, fund=null)}, authors=[Author(id=1322922867721122664, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922866353779548, orderNo=0, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1322922867834368874, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922866353779548, authorId=1322922867721122664, language=EN, stringName=Yuanjin SU, firstName=Yuanjin, middleName=null, lastName=SU, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=¹, address=^1.College of Pharmacy，Gansu University of Chinese Medicine，Lanzhou 730000，Gansu，China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1322922867905672043, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922866353779548, authorId=1322922867721122664, language=CN, stringName=苏圆锦, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=¹, address=^1.甘肃中医药大学药学院，甘肃 兰州 730000, bio={"content":"

苏圆锦（1999-），女，陕西咸阳人，硕士，研究方向：中药鉴定与品质评价。

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苏圆锦（1999-），女，陕西咸阳人，硕士，研究方向：中药鉴定与品质评价。

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Optom Vis Sci，2011，88（2）：257-262., articleTitle=Goji berry effects on macular characteristics and plasma antioxidant levels, refAbstract=null), Reference(id=1322922881084175312, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922866353779548, doi=null, pmid=null, pmcid=null, year=2002, volume=25, issue=3, pageStart=390, pageEnd=392, url=null, language=null, rfNumber=［55］, rfOrder=54, authorNames=KIM H P, LEE E J, KIM Y C, journalName=Biol Pharm Bull, refType=null, unstructuredReference=KIM H P，LEE E J，KIM Y C，et al. Zeaxanthin dipalmitate from Lycium chinense fruit reduces experimentally induced hepatic fibrosis in rats［J］. Biol Pharm Bull，2002，25（3）：390-392., articleTitle=Zeaxanthin dipalmitate from Lycium chinense fruit reduces experimentally induced hepatic fibrosis in rats, refAbstract=null), Reference(id=1322922881201615825, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922866353779548, doi=null, pmid=null, pmcid=null, year=2014, volume=9, issue=4, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=［56］, rfOrder=55, authorNames=XIAO J, WANG J T, XING F Y, journalName=PLoS One, refType=null, unstructuredReference=XIAO J，WANG J T，XING F Y，et al. Zeaxanthin dipalmitate therapeutically improves hepatic functions in an alcoholic fatty liver disease model through modulating MAPK pathway［J］. PLoS One，2014，9（4）：e95214., articleTitle=Zeaxanthin dipalmitate therapeutically improves hepatic functions in an alcoholic fatty liver disease model through modulating MAPK pathway, refAbstract=null), Reference(id=1322922881436496850, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922866353779548, doi=null, pmid=null, pmcid=null, year=2009, volume=54, issue=7, pageStart=1460, pageEnd=1464, url=null, language=null, rfNumber=［57］, rfOrder=56, authorNames=CHAMBERLAIN S M, HALL J D, PATEL J, journalName=Dig Dis Sci, refType=null, unstructuredReference=CHAMBERLAIN S M，HALL J D，PATEL J，et al. Protective effects of the carotenoid Zeaxanthin in experimental nonalcoholic steatohepatitis［J］. Dig Dis Sci，2009，54（7）：1460-1464., articleTitle=Protective effects of the carotenoid Zeaxanthin in experimental nonalcoholic steatohepatitis, refAbstract=null), Reference(id=1322922881520382931, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922866353779548, doi=null, pmid=null, pmcid=null, year=2017, volume=19, issue=9, pageStart=910, pageEnd=923, url=null, language=null, rfNumber=［58］, rfOrder=57, authorNames=LI J J, GAO H, LV Y, journalName=J Asian Nat Prod Res, refType=null, unstructuredReference=LI J J，GAO H，LV Y，et al. Zeaxanthin dipalmitate alleviates hepatic injury induced by superimposed chronic hepatitis B and nonalcoholic steatohepatitis in non-obese mice［J］. 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Mol Nutr Food Res，2019，63（11）：e1801339., articleTitle=Wolfberry-derived Zeaxanthin dipalmitate attenuates ethanol-induced hepatic damage, refAbstract=null)], funds=[Fund(id=1322922872439714711, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922866353779548, awardId=21YF5GA134, language=CN, fundingSource=甘肃省科技计划项目重点研发计划项目(21YF5GA134), fundOrder=null, country=null), Fund(id=1322922872519406488, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922866353779548, awardId=2022-RC-29, language=CN, fundingSource=兰州市人才创新创业项目(2022-RC-29), fundOrder=null, country=null)], companyList=[AuthorCompany(id=1322922867507213153, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922866353779548, xref=1., ext=[AuthorCompanyExt(id=1322922867515601762, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922866353779548, companyId=1322922867507213153, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=^1.College of Pharmacy，Gansu University of Chinese Medicine，Lanzhou 730000，Gansu，China), AuthorCompanyExt(id=1322922867523990371, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922866353779548, companyId=1322922867507213153, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=^1.甘肃中医药大学药学院，甘肃 兰州 730000)]), AuthorCompany(id=1322922867599487844, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922866353779548, xref=2., ext=[AuthorCompanyExt(id=1322922867612070757, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922866353779548, companyId=1322922867599487844, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=^2.Lanzhou Institute of Chemical Physics，Chinese Academy of Sciences，Lanzhou 730000，Gansu，China), AuthorCompanyExt(id=1322922867620459366, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922866353779548, companyId=1322922867599487844, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=^2.中国科学院兰州化学物理研究所，甘肃 兰州 730000)])], figs=[ArticleFig(id=1322922870095098769, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922866353779548, language=EN, label=null, caption=null, figureFileSmall=AacfR8s4HkePUL6bMa98sw==, figureFileBig=6efGv2VTXjHGeSOFV21pIg==, tableContent=null), ArticleFig(id=1322922870170596242, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922866353779548, language=CN, label=图1, caption=枸杞中主要色素类成分结构, figureFileSmall=AacfR8s4HkePUL6bMa98sw==, figureFileBig=6efGv2VTXjHGeSOFV21pIg==, tableContent=null), ArticleFig(id=1322922872011895699, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922866353779548, language=EN, label=null, caption=null, figureFileSmall=null, figureFileBig=null, tableContent=

序号	化合物名称	文献
1	β-胡萝卜素	[8]
2	玉米黄素	[8]
3	叶黄素	[8]
4	β-隐黄素	[8]
5	玉米黄素单豆蔻酸酯	[9]
6	玉米黄素单棕榈酸酯	[10]
7	玉米黄素二棕榈酸酯	[10]
8	β-隐黄素单棕榈酸酯	[10]
9	紫黄素二棕榈酸酯	[9]
10	玉米黄素二棕榈酸酯	[9]
11	花药黄素二棕榈酸酯	[9]

), ArticleFig(id=1322922872083198868, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922866353779548, language=CN, label=表1, caption=

枸杞中主要的色素类化合物

, figureFileSmall=null, figureFileBig=null, tableContent=

序号	化合物名称	文献
1	β-胡萝卜素	[8]
2	玉米黄素	[8]
3	叶黄素	[8]
4	β-隐黄素	[8]
5	玉米黄素单豆蔻酸酯	[9]
6	玉米黄素单棕榈酸酯	[10]
7	玉米黄素二棕榈酸酯	[10]
8	β-隐黄素单棕榈酸酯	[10]
9	紫黄素二棕榈酸酯	[9]
10	玉米黄素二棕榈酸酯	[9]
11	花药黄素二棕榈酸酯	[9]

), ArticleFig(id=1322922872171279253, tenantId=1146029695717560320, journalId=1255847919539208197, articleId=1322922866353779548, language=EN, label=null, caption=null, figureFileSmall=null, figureFileBig=null, tableContent=

提取方法	考察因素	考察方法	影响因子程度大小	最佳工艺	最大色素提取率/（%）	色素（类胡萝卜素）含量	文献
溶剂提取法	提取温度（A）、提取时间（B）、液料比（C）、石油醚？丙酮（D）	二次正交旋转实验设计	B>C>A>D	A（30.9℃）、B（2.2 h）、C（7.16∶1）、D（5.43∶1）	—	—	[17]
溶剂提取法	提取温度（A）、提取时间（B）、乙醇浓度（C）、提取次数（D）	单因素实验+正交实验	C>A>D>B	A（80℃）、B（2 h）、C（60%）、D（3次）	49.1	—	[11]
溶剂提取法	提取温度（A）、提取时间（B）、提取次数（C）、液料比（D）	单因素实验+正交实验	A>C>B>D	A（50℃）、B（1.5 h）、C（4次）、D（3∶1）	91.6	—	[18]
溶剂提取法	提取温度（A）、提取时间（B）、乙醇浓度（C）、料液比（D）	单因素实验+正交实验	C>A>D>B	A（50℃）、B（1 h）、C（无水乙醇）、D（1∶12）	8.1	—	[19]
溶剂提取法	提取温度（A）、提取时间（B）、pH值（C）、提取溶剂（D）	正交实验	D>A>C>B	A（50℃）、B（4 h）、C（3）、D（异丙醇）	60	—	[20]
溶剂提取法	提取温度（A）、提取时间（B）、料液比（C）、甲醇∶无水乙醇（D）	正交试验	D>A>B>C	A（60℃）、B（60 min）、C（1∶10）、D（1∶3）	—	69.58 mg/100 g	[21]
	提取温度（A）、提取时间（B）、料液比（C）、无水乙醇∶石油醚（D）	正交试验	C>A>B>D	A（40℃）、B（50 min）、C（1∶10）、D（1∶2）	—	69.58 mg/100 g
	提取温度（A）、提取时间（B）、料液比（C）、无水乙醇∶乙酸乙酯（D）	正交试验	C>A>B>D	A（40℃）、B（60 min）、C（1∶10）、D（1∶2）	—	286.75 mg/100 g
	提取温度（A）、提取时间（B）、料液比（C）、无水乙醇∶石油醚（D）	响应面分析	A>B>C>D	A（45℃）、B（50 min）、C（1∶12.5）、D（1∶1）	—	327.57 mg/100 g
超声波辅助提取法	无水乙醇∶石油醚（A）、料液比（B）、提取时间（C）	单因素实验+响应面分析	A>C>B	A（2.6∶1）、B（1∶60）、C（41 min）	—	321.52 mg/100 g	[22]
超声波辅助提取法	提取温度（A）、提取时间（B）、料液比（C）、超声功率（D）	单因素实验+正交实验	A>C>D>B	A（40℃）、B（25 min）、C（1∶6）、D（80 W）	97.68	—	[13]
超声波辅助提取法	提取温度（A）、提取时间（B）、料液比（C）、超声功率（D）	单因素实验+正交实验	A>C>B>D	A（40℃）、B（50 min）、C（1∶30）、D（200 W）	9.93	—	[23]
超声波辅助提取法	提取时间（A）、料液比（B）、粒度（C）	正交试验	C>A>B	A（30 min）、B（1∶15）、C（>40目）	92.3	—	[24]
超声波辅助提取法	提取时间（A）、超声功率（B）、料液比（C）、超声频率（D）	单因素实验+正交实验	B>A>C>D	A（50 min）、B（300W）、C（1∶15）、D（60 Hz）	—	18.16 mg/100 g	[25]
微波辅助法	提取温度（A）、提取时间（B）、料液比（C）、提取次数（D）	单因素实验+正交实验	C>D>A>B	A（45℃）、B（20 min）、C（1∶30）、D（2次）	17.14	—	[14]
微波辅助法	微波火力（A）、提取时间（B）、料液比（C）、提取次数（D）	单因素实验+正交实验	D>C>B>A	A（中火）、B（20 S）、C（1∶4）、D（3次）	—	14.23 mg/L	[26]
微波辅助法	微波功率（A）、提取时间（B）、料液比（C）、0.1 mol/L HCl∶95%乙醇（D）	单因素实验+正交实验	D>B>A>C	A（400 W）、B（30S）、C（1∶10）、D（2∶3）	16.86	—	[27]
皂化提取法	皂化温度（A）、皂化时间（B）、NaOH质量分数（C）、液料比（D）	单因素实验+正交实验	A>C>D>B	A（40℃）、B（40 min）、C（1.0%）、D（4∶1）	—	—	[12]
皂化提取法	提取温度（A）、提取时间（B）、Na2CO3浓度（C）、起泡pH值（D）	单因素实验+正交实验	A>C>B>D	A（40℃）、B（3 h）、C（0.7 mol/L）、D（7.5）	—	—	[28]
超声-微波协同提取法	料液比（A）、微波功率（B）、提取温度（C）、提取时间（D）	单因素实验+响应面分析	B>A>C>D	A（1∶16.2）、B（612.7 W）、C（55.39℃）、D（31.76 min）	—	—	[29]
超声波辅助皂化法	皂化温度（A）、皂化时间（B）、KOH浓度（C）、超声功率（D）	单因素实验+响应面分析	B>A>C>D	A（40.7℃）、B（1 h）、C（1.8 mol/L）、D（205 W）	—	128.099 mg/100 g	[30]
酶协同超声波法	酶解温度（A）、酶解pH（B）、液料比（C）	单因素实验+响应面分析	B>C>A	A（35.5℃）、B（5.5）、C（15.5∶1）	—	—	[16]

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枸杞色素的提取方法及结果

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提取方法	考察因素	考察方法	影响因子程度大小	最佳工艺	最大色素提取率/（%）	色素（类胡萝卜素）含量	文献
溶剂提取法	提取温度（A）、提取时间（B）、液料比（C）、石油醚？丙酮（D）	二次正交旋转实验设计	B>C>A>D	A（30.9℃）、B（2.2 h）、C（7.16∶1）、D（5.43∶1）	—	—	[17]
溶剂提取法	提取温度（A）、提取时间（B）、乙醇浓度（C）、提取次数（D）	单因素实验+正交实验	C>A>D>B	A（80℃）、B（2 h）、C（60%）、D（3次）	49.1	—	[11]
溶剂提取法	提取温度（A）、提取时间（B）、提取次数（C）、液料比（D）	单因素实验+正交实验	A>C>B>D	A（50℃）、B（1.5 h）、C（4次）、D（3∶1）	91.6	—	[18]
溶剂提取法	提取温度（A）、提取时间（B）、乙醇浓度（C）、料液比（D）	单因素实验+正交实验	C>A>D>B	A（50℃）、B（1 h）、C（无水乙醇）、D（1∶12）	8.1	—	[19]
溶剂提取法	提取温度（A）、提取时间（B）、pH值（C）、提取溶剂（D）	正交实验	D>A>C>B	A（50℃）、B（4 h）、C（3）、D（异丙醇）	60	—	[20]
溶剂提取法	提取温度（A）、提取时间（B）、料液比（C）、甲醇∶无水乙醇（D）	正交试验	D>A>B>C	A（60℃）、B（60 min）、C（1∶10）、D（1∶3）	—	69.58 mg/100 g	[21]
	提取温度（A）、提取时间（B）、料液比（C）、无水乙醇∶石油醚（D）	正交试验	C>A>B>D	A（40℃）、B（50 min）、C（1∶10）、D（1∶2）	—	69.58 mg/100 g
	提取温度（A）、提取时间（B）、料液比（C）、无水乙醇∶乙酸乙酯（D）	正交试验	C>A>B>D	A（40℃）、B（60 min）、C（1∶10）、D（1∶2）	—	286.75 mg/100 g
	提取温度（A）、提取时间（B）、料液比（C）、无水乙醇∶石油醚（D）	响应面分析	A>B>C>D	A（45℃）、B（50 min）、C（1∶12.5）、D（1∶1）	—	327.57 mg/100 g
超声波辅助提取法	无水乙醇∶石油醚（A）、料液比（B）、提取时间（C）	单因素实验+响应面分析	A>C>B	A（2.6∶1）、B（1∶60）、C（41 min）	—	321.52 mg/100 g	[22]
超声波辅助提取法	提取温度（A）、提取时间（B）、料液比（C）、超声功率（D）	单因素实验+正交实验	A>C>D>B	A（40℃）、B（25 min）、C（1∶6）、D（80 W）	97.68	—	[13]
超声波辅助提取法	提取温度（A）、提取时间（B）、料液比（C）、超声功率（D）	单因素实验+正交实验	A>C>B>D	A（40℃）、B（50 min）、C（1∶30）、D（200 W）	9.93	—	[23]
超声波辅助提取法	提取时间（A）、料液比（B）、粒度（C）	正交试验	C>A>B	A（30 min）、B（1∶15）、C（>40目）	92.3	—	[24]
超声波辅助提取法	提取时间（A）、超声功率（B）、料液比（C）、超声频率（D）	单因素实验+正交实验	B>A>C>D	A（50 min）、B（300W）、C（1∶15）、D（60 Hz）	—	18.16 mg/100 g	[25]
微波辅助法	提取温度（A）、提取时间（B）、料液比（C）、提取次数（D）	单因素实验+正交实验	C>D>A>B	A（45℃）、B（20 min）、C（1∶30）、D（2次）	17.14	—	[14]
微波辅助法	微波火力（A）、提取时间（B）、料液比（C）、提取次数（D）	单因素实验+正交实验	D>C>B>A	A（中火）、B（20 S）、C（1∶4）、D（3次）	—	14.23 mg/L	[26]
微波辅助法	微波功率（A）、提取时间（B）、料液比（C）、0.1 mol/L HCl∶95%乙醇（D）	单因素实验+正交实验	D>B>A>C	A（400 W）、B（30S）、C（1∶10）、D（2∶3）	16.86	—	[27]
皂化提取法	皂化温度（A）、皂化时间（B）、NaOH质量分数（C）、液料比（D）	单因素实验+正交实验	A>C>D>B	A（40℃）、B（40 min）、C（1.0%）、D（4∶1）	—	—	[12]
皂化提取法	提取温度（A）、提取时间（B）、Na2CO3浓度（C）、起泡pH值（D）	单因素实验+正交实验	A>C>B>D	A（40℃）、B（3 h）、C（0.7 mol/L）、D（7.5）	—	—	[28]
超声-微波协同提取法	料液比（A）、微波功率（B）、提取温度（C）、提取时间（D）	单因素实验+响应面分析	B>A>C>D	A（1∶16.2）、B（612.7 W）、C（55.39℃）、D（31.76 min）	—	—	[29]
超声波辅助皂化法	皂化温度（A）、皂化时间（B）、KOH浓度（C）、超声功率（D）	单因素实验+响应面分析	B>A>C>D	A（40.7℃）、B（1 h）、C（1.8 mol/L）、D（205 W）	—	128.099 mg/100 g	[30]
酶协同超声波法	酶解温度（A）、酶解pH（B）、液料比（C）	单因素实验+响应面分析	B>C>A	A（35.5℃）、B（5.5）、C（15.5∶1）	—	—	[16]

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