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Stem cells from human exfoliated deciduous teeth are widely used in the field of tissue repair and regeneration, but the tissue regeneration effect has limitations in practical applications. Using miRNA to intervene in the directional differentiation of stem cells from human exfoliated deciduous teeth is an important development direction for tissue repair and regeneration in the future.
To investigate the effect of miR-26b on the neurogenic and angiogenic differentiation of stem cells from human exfoliated deciduous teeth.
Dental pulp stem cells were isolated and extracted from human exfoliated deciduous teeth, and induced to differentiate into nerves and blood vessels. The expressions of neurogenic markers Nestin, NSE, βIII-Tubulin, and angiogenic markers CD31, VEGFR2, ANG-1 and miR-26b were detected. Dental pulp stem cells were divided into blank control group, miR-26 overexpression group, and negative control group. RT-qPCR, cell immunofluorescence staining, and western blot assay were used to detect the expression changes of related markers of neurogenic and angiogenic induction of stem cells from human exfoliated deciduous teeth in each group.
(1) Stem cells from human exfoliated deciduous teeth had multi-directional differentiation potential and could differentiate into osteogenesis, adipogenesis, neurogenesis, and vasculogenesis. (2) The expression level of miR-26b increased during the neurogenic and angiogenic differentiation of stem cells from human exfoliated deciduous teeth. (3) Compared with the blank control group and negative control group, the mRNA expression of neuroblast-related genes βIII-Tubulin, Nestin, NSE and angiogenesis-related genes CD31, VEGFR2, and ANG-1 in stem cells from human exfoliated deciduous teeth in the miR-26b overexpression group was significantly increased (P < 0.01), βIII-Tubulin, Nestin, CD31, and VEGFR2 protein expression was significantly increased (P < 0.01). The above results show that overexpression of miR-26b can promote the neurogenic and angiogenic differentiation of stem cells from human exfoliated deciduous teeth.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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