PDF
Full
Outline
OBJECTIVE To screen and study the effects of small activating RNA (saRNA) enhancing the expression of ABCG2 gene on renal and intestinal cell excretion of uric acid (UA) to explore a new type of therapeutic mechanism drugs to promote UA excretion. METHODS saRNAs targeting mice and human ABCG2 genes were designed and screened by real-time quantitative reverse transcription PCR(RT-qPCR) and Western blot in mice TCMK-1 and human HK-2 cells. The effect of the selected saRNA on the excretion of UA was examined in TCMK-1 and HK-2 cells. The mice saRNA was injected into the tail vein of hyperuricemia (HUA) model mice. The levels of serum uric acid (SUA), urinary uric acid (UUA), intestinal uric acid, blood urea nitrogen (BUN) and serum creatinine (SCr), and the activity of liver xanthine oxidase (XOD) in the mice were detected to verify the ability of selected saRNA to promote UA excretion. The pathological changes of kidney and small intestine were analyzed through H&E. The expression of ABCG2 in kidney and small intestine was analyzed by RT-qPCR, Western blot and immunohistochemistry. RESULTS Four saRNAs targeting ABCG2 were selected, 2 for mice and 2 for human, named saRNA-1/2 and hsaRNA-1/2 respectively, which increased the expression of ABCG2 in TCMK-1 and HK-2 cells (P<0.05), and promoted the extracellular excretion of UA (P<0.05). In HUA model mice, saRNA-1/2 increased the levels of UUA and intestinal UA (P<0.05), reduced the levels of SUA, BUN, and SCr (P<0.05), decreased the damage of HUA to kidney and small intestine, and enhanced the expression of ABCG2 in kidney and small intestine, but did not show significant effect on the activity of liver XOD (P>0.05). CONCLUSION The selected saRNAs could enhance the expression of ABCG2 and promote the excretion of UA in cells and mice, reducing SUA level.
PDF
78
42
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62199257
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
