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Establishment and Application of TaqMan Real-Time PCR Method for Periplaneta americana
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Li LIU1, Yingying XIE2, Qian SUN2, Lin LIN2, Lejun TAN2, Juan CHEN1, Shuxiu ZHAO1, Yongqiang LIN2, *, Bing WANG2, *
Chinese Pharmaceutical Journal | 2024, 59(5) : 409 - 415
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Chinese Pharmaceutical Journal | 2024, 59(5): 409-415
Establishment and Application of TaqMan Real-Time PCR Method for Periplaneta americana
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Li LIU1, Yingying XIE2, Qian SUN2, Lin LIN2, Lejun TAN2, Juan CHEN1, Shuxiu ZHAO1, Yongqiang LIN2, *, Bing WANG2, *
Affiliations
  • 1 Shandong University of Traditional Chinese Medicine, Jinan 250355, China
  • 2 NMPA Key Laboratory for Quality Evaluation of Gelatin Products, Shandong Engineering Laboratory for Standard Innovation and Quality Evaluation of TCM, Shandong Institute for Food and Drug Control, Jinan 250101, China
Published: 2024-03-08
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OBJECTIVE To establish a highly specific TaqMan real-time PCR method for the identification of Periplaneta americana medicinal materials and Periplaneta americana powder.METHODS According to the characteristics of genome sequence of Periplaneta americana, specific primers and probes suitable for real-time PCR detection were designed, and the probe concentration, cycle times and annealing temperature were optimized to establish the best TaqMan real-time PCR method of Periplaneta americana. RESULTS The specific primers and probes were only specifically amplified from Periplaneta americana medicinal materials and Periplaneta americana powder, and no fluorescence curves were amplified from Blaptica dubia, Eupolyphaga Sinensis W. Blattella germanica and Aspongopus chinensis Dallas. CONCLUSION The established TaqMan real-time PCR method can accurately and rapidly identify Periplaneta americana and common counterfeit products, providing a more specific technical reference for the identification of Periplaneta americana.

Periplaneta americana  /  real-time PCR  /  TaqMan probe  /  herbal decoction piece
Li LIU, Yingying XIE, Qian SUN, Lin LIN, Lejun TAN, Juan CHEN, Shuxiu ZHAO, Yongqiang LIN, Bing WANG. Establishment and Application of TaqMan Real-Time PCR Method for Periplaneta americana[J]. Chinese Pharmaceutical Journal, 2024 , 59 (5) : 409 -415 .
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Article Info
  • Receive Date：2023-06-12
  • Online Date：2026-04-08
  • Published：2024-03-08
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  • Received：2023-06-12
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Affiliations
    1 Shandong University of Traditional Chinese Medicine, Jinan 250355, China
    2 NMPA Key Laboratory for Quality Evaluation of Gelatin Products, Shandong Engineering Laboratory for Standard Innovation and Quality Evaluation of TCM, Shandong Institute for Food and Drug Control, Jinan 250101, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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