OBJECTIVE To explore the effects of different drying methods on multiple index constituents in Lysimachiae Herba. METHODS A comprehensive analytical method employed with ultra high performance liquid chromatography with triple quadrupole linear ion trap mass spectrometry (UHPLC-QTRAP-MS/MS) was used for simultaneous determination of forty-four constituents including flavonoids, organic acids, amino acids, and nucleosides in Lysimachiae Herba. The hierarchical cluster analysis (HCA), gray relation analysis (GRA), and technique for order preference by similarity to ideal solution(TOPSIS) methods were applied to comprehensively analyze and evaluate the results of multiple index constituents. RESULTS HCA results showed that seven different drying methods were mainly divided into two categories, the first category was sun drying and shady drying, and the second category was drying at different temperatures, microwave drying, and freeze drying. In addition, GRA and TOPSIS results showed that sun drying samples had better quality, and shady drying and freeze drying could be used as alternative drying methods. CONCLUSION This study can provide a basis for revealing the scientific connotation of traditional drying method of Lysimachiae Herba, and provide information for the optimization of drying method during the processing of Lysimachiae Herba.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |