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Viable Bacteria Content and Gene Safety Analysis of Bifidobacterium Microbial Products
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Xiaoling ZHENG1, Yinhuan WANG1, Junhao CHEN1, Ling QIAN1, Wei CAO1, Xin WU1, Jue LI1, Wanzi GONG2, Jing QIAN3, *
Chinese Pharmaceutical Journal | 2024, 59(13) : 1246 - 1251
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Chinese Pharmaceutical Journal | 2024, 59(13): 1246-1251
Viable Bacteria Content and Gene Safety Analysis of Bifidobacterium Microbial Products
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Xiaoling ZHENG1, Yinhuan WANG1, Junhao CHEN1, Ling QIAN1, Wei CAO1, Xin WU1, Jue LI1, Wanzi GONG2, Jing QIAN3, *
Affiliations
  • 1 NMPA Key Laboratory for Testing and Risk Warning of Pharmaceutical Microbiology, Key Laboratory of Drug Contacting Materials Quality Control of Zhejiang Provincial, Zhejiang Institute for Food and Drug Control, Hangzhou 310004, China
  • 2 China Pharmaceutical University, Nanjing 211198, China
  • 3 Zhejiang Center for Drug and Cosmetic Evaluation, Hangzhou 310012, China
Published: 2024-07-08 doi: 10.11669/cpj.2024.13.010
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OBJECTIVE To investigate the influencing factors and test methods of viable bacterial count in probiotic products of nature Bifidobacterium, and provide reference and suggestions for improving the quality control of such products. METHODS The effects of storage temperature and viable count determination method on viable count were studied, and the pretreatment methods and diluent composition were optimized, and the drug resistance genes and virulence genes of 11 strains contained in four products were analyzed by whole genome sequencing and functional genomics analysis. RESULTS The results showed that temperature had a great influence on the results of viable bacteria count of Bifidobacteria products, and the optimized method of live bacteria count could effectively improve the count results. Several strains of live bacteria products contained drug resistance genes and virulence genes. CONCLUSION It is suggested that we should pay attention to the influence of temperature on products in production storage and circulation. The count method of live bacteria can consider the characteristics of strains and production technology, and optimize the influencing factors such as pre-treatment and diluent. And there is a need for systematic safety assessment of all strains involved in commercially live bacteria product.

Bifidobacterium  /  viable bacterial count  /  drug resistance gene  /  virulence gene  /  live bacteria product
Xiaoling ZHENG, Yinhuan WANG, Junhao CHEN, Ling QIAN, Wei CAO, Xin WU, Jue LI, Wanzi GONG, Jing QIAN. Viable Bacteria Content and Gene Safety Analysis of Bifidobacterium Microbial Products[J]. Chinese Pharmaceutical Journal, 2024 , 59 (13) : 1246 -1251 . DOI: 10.11669/cpj.2024.13.010
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Article Info
doi: 10.11669/cpj.2024.13.010
  • Receive Date：2023-09-25
  • Online Date：2026-01-14
  • Published：2024-07-08
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  • Received：2023-09-25
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Affiliations
    1 NMPA Key Laboratory for Testing and Risk Warning of Pharmaceutical Microbiology, Key Laboratory of Drug Contacting Materials Quality Control of Zhejiang Provincial, Zhejiang Institute for Food and Drug Control, Hangzhou 310004, China
    2 China Pharmaceutical University, Nanjing 211198, China
    3 Zhejiang Center for Drug and Cosmetic Evaluation, Hangzhou 310012, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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