PDF
Full
Outline
OBJECTIVE To establish a method based on gas chromatography-mass spectrometry (GC-MS) for simultaneous determination of a variety of soluble sugars in Ophiopohon japonicus tuberous roots and fibrous roots and to analyze the differences in the soluble sugars by multivariate statistical analysis. METHODS The optimized two-step derivatization method of methoxylation-trimethylsilylation combined with GC-MS was used to determine the soluble sugar components in 18 batches of Ophiopohon japonicus tuberous roots and fibrous roots. And the differences in the components were analyzed by multivariate statistical analysis, and the sweetness values of the tuberous roots and fibrous roots samples were converted according to the contents. RESULTS Eighteen kinds of soluble sugars were identified and 14 kinds of them were quantitatively analyzed in the tuberous roots and fibrous roots of Ophiopohon japonicus. It was found that the ingredients of the soluble sugar components in the tuberous roots and fibrous roots of Ophiopohon japonicus were basically the same, and the average of the total contents of the 14 kinds of soluble sugar components were also basically the same (88.76 mg·g-1 in tuberous roots vs. 85.66 mg·g-1 in fibrous roots). However, there were some differences in the contents of the components, and the contents of D-(-)-fructose and in the fibrous roots were significantly higher than those in the tuberous roots, while the contents of D-(+)-sucrose in the tuberous roots were significantly higher than those in the fibrous roots. The results of multivariate statistical analysis showed that tuberous roots and fibrous roots could be significantly differentiated by the determination of soluble sugar fractions. The difference in sweetness values between tuberous roots and fibrous roots was small by conversion. CONCLUSION With high sensitivity, good precision and accuracy, GC-MS is able to distinguish a variety of soluble monosaccharides and disaccharides with similar structures, and realize the accurate characterization and quantification of a variety of soluble sugars in Ophiopohon japonicus, and providing a basis for the study of soluble sugar fractions in different parts of Ophiopohon japonicus and its further development and utilization.
PDF
92
43
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62199257
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
